Gene:
Gene Symbol:
Pard3b
Gene Name:
par-3 family cell polarity regulator beta
Original Clone ID:
FLH481620.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295373
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3429nts
Open reading frame: 35 to 3463
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAAGTGACAGTGTGCTTCGGGAG
GACGGGCATCGTGGTGCCCTGCAAGGACGGCCAGCTCCGGGTCCGCGAGCTCACCCAGCA
GGCGCTGCAGCGGTACCTGAAGACCCGGGACCAGGATCCTGGATACTGGGTGAAGATTCA
TCACTTAGAATACACAGATGGAGGAATCCTGGATCCGGATGATGTCTTAGCAGATGTTGT
TGAAGACAAAGATAAGGTACTGATTGCTGTGTTTGACGAACAAGAGCCACTCCAAAAGAC
TGAGAGCCCCGGTGGAAACCCTGCGGATCGGCAGAGCCCAGATGCTTTCGAGACAGAAGT
GGCTGCCCAACTGGCTGCATTTAAACCTGTCGGTGGGGAAATCGTAGTAACCCCTTCTGC
TCTGAAGTTAGGCACTCCACTGCTGGTTAGGAGAAGCAGTGACCCAGCACCAGGGCCCCA
TGCTGATGCCCAGCCAAGCACTGCAAGCCTGAGTGGCCAGAGTTTGAAACCTGTTGTTCT
GGATTCCACCCAGAACGTGGAAAACAAAGAAGCTATGAATGGTGAACAGGCAGGGCTGCT
TTCACTGCACAGACCGAAGGATGAGCTGAGTGATATGACGAGAGCTGTGGAGATTTCTGG
GGAAGGAGACCCCCTGGGGATCCACGTGGTGCCCTTCTTTTCATCCCTGAGTGGCCGGAT
CCTAGGACTCTTCATCCGAGGCATTGAAGAAAACAGCAGGTGCAAGCAGGAGGGACTATT
TCAGGAGAATGAGTGCATTGTAAAAATCAACAATGTGGAACTCCTGGACAAAACTTTTGC
TCAGGCCCAAGATGTCTTCCGCCAGGCAATGAAATCTCCCAGTGTGATTCTCCATGTGCT
TCTGCCTCAAAACCGTGAACAATATGAAAAATCAGTCATTGGACCACTCAACATTTTTGG
CAACAATGATGGGGCTTCGAGAACAAAGGCAGCACCTCCTGCCCGTGGGAAACCAGGTCT
GAAGGCAGTACATCTAACAAGAGCGAGCAGCCCTGAAGGAGAAGAGCCAGCCTCCCCGCA
GCAAAGCAAGAGTCCCCGGGTTCCAAGACTAGGAAGAAAACCTTCCTCTCCCTCACTCTC
TCCTCTCATGGGGTTTGGCAGCAAGAAAAATGCAAAAAAAATTAAGATTGACCTAAAGAA
AGGCCCTGAGGGACTTGGTTTCACTGTGGTAACCAGAGATTCTTCTATACATGGTCCTGG
TCCCATTTTTGTAAAAAACATCTTACCAAAAGGAGCAGCAGTAAAGGATGGCCGCCTACA
ATCAGGGGACAGAATTTTGGAGGTAAATGGCAGAGATGTTACAGGAAGAACCCAAGAAGA
ACTCGTGGCCATGTTAAGGAGCACCAAGCAGGGAGAGACAGTGTCATTGGTCATTGCCCG
CCAGGAAGGGAGCTTCCTGCCCCGAGAGCTGGATGGCCGTCTCCGAATGAATGACCAACT
GATTGCTGTTAACGGGGAAACTCTTCTGGGAAAGTCCAACCACGAAGCCATGGAAACACT
CAGGCGATCCATGTCCATGGAGGGAAACATCCGGGGCATGATCCAGTTGGTGATTCTGAG
GAGACCTGAGAGACCACTGGAGGAGCTTTCAGAATGTGGAGCACTTTCCAGACCAGGCTT
TGAGAACTGTCAAGAAGCTCTGAGCACCTCCAGGCGAAATGATAGTAGTATATTATATCC
GTTTGGCACCTATAGTCCACAAGATAAAAGGAAAGACCTATTGCTTCCCAGTGATGGGTG
GGCTGAGAATGAAGTACCACCGTCCCCGCCACCACATCCTGCTCTGGAATGGGGCCTTGA
AGATTTCAGTCACAGCTCTGGAGTGGATTCCACAGGATATTTTCCAGATCAACATGTCAA
CTTCAGAACGGTGACACCAGTCAGGCAGCCCGAATTGATTAACCTTAAAGCCTCCAAGAG
CATGGACCTTGTGCCAGATGAAGGCAAAGTTCAGTCGCTGGCGGATCGCAGATCAGACTC
TCCAGGCAAAGATTTTGGTCCAACTTTGGGTCTGAAAAAGTCCAGTTCCTTGGAGAGCCT
ACAGACGGCTGTGGCTGAAGTCAGGAAGAATGATCTGCCCTTCCACAGGCCCAGACCACA
CATGGTGCGGGGCCGAGGCTGCAACGAGAGCTTCCGAGCAGCCATCGACAAATCGTACGA
TGGGCCAGAAGAGGCAGACGCGGATGGTCTGTCTGATAAGAGCTCTCGCTCAGGCCACAC
AGCTCTGAATTGTGAGTCTGCCCCTCAGGGAAACCCCGAGCTAGACAATGTGGAAAATAA
AGCCAAAAACATCAAAAAAACAAAAGAGAAGGAGAAGAAAAAAGGAAAGGGCAAGCTGAA
AGTCAAGGAAAAAAAGCTGAAGGAGGAACATGAGGACGCAGAGAGGAAAATGAAGAGGAA
GGGCTTCGGTGCCATGCTGAGATTTGGAAAGAAGAAAGATGATAAGGTCGGAAAGGCTGA
GCAGAAAGGTGCGCAGAAGTCCGGCCACCCGGAGGAAGAGGAGCTGGAAAGAATGAAAGA
AGAGAGAGAGAGGATTGGAGCAAAACATCAGGAGCTGAGAGAAAAGCAGGCAAGAGGCCT
TGTTGATTATGCCACGGCAGTGACTGGACCAGTGCACGATATGGACGATGACGAAATGGA
CCCCAATTATGCCAGAGTGAACCACTTTCGGGAACCATGTGCATCAGCAAATGTCTTTAG
ATCTCCATCTCCCCTTCGAGCTGGACCACTTGCTTACCCTCGGGATGGGCGTCCACTGTC
TCCAGACCACTTAGAGGGTCTCTATGCCAAGGTCAACAAGCCATATCATCCACCAGCCCT
AGCTGACAGTGGCCGACCCATGGCTGGAACCACGGACCGCATCCAGAAGCTTCGGAAGGA
GTATTACCAGGCTCGGAGAGAAGGGTTCCTTTTGTATGAAGATGAAAACACAAGAGCACG
GCCATCTGACCATGACCTCCGCTGGGTGTCTGGAAAGGGTCCAGATGGGAGCACACACAA
CCTCCGCTTTGAGGGGATGGAGCGGCAGTACGCATCCTTACCCAGGGGAGGCTCAGCAGA
TCCCGTGGACTACCTGACAGCTTCACCCCGAGGGCGCTACAATGACAGAGAGCTTCCATA
CTACCCAGGGCCTCATCCTGTACATGCCCCAAGGGGGAGCTATCCCCGCCCCCCAGATCT
GAGGGCAACAGATCTACGATACCCACAGTACTACCCACCCCCACCAGCCCATCAGCACAA
AGGGCCCTTCAGACAAGATGTTCCTCCTTCACCACCTCAGCACCAGCGAGTACCGGTCTA
CCAGGAGATGGGCAGAGCAGGGCCCCGAGGGAGCAGCCCTGACCAATACCCCTATCGTAA
CCAGGATCCCAGGCAGAAGAATCCCATGACTGCAGCGGTATAGGGCCTCATGGGCCCAGC
TTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MKVTVCFGRTGIVVPCKDGQLRVRELTQQALQRYLKTRDQDPGYWVKIHHLEYTDGGILD PDDVLADVVEDKDKVLIAVFDEQEPLQKTESPGGNPADRQSPDAFETEVAAQLAAFKPVG GEIVVTPSALKLGTPLLVRRSSDPAPGPHADAQPSTASLSGQSLKPVVLDSTQNVENKEA MNGEQAGLLSLHRPKDELSDMTRAVEISGEGDPLGIHVVPFFSSLSGRILGLFIRGIEEN SRCKQEGLFQENECIVKINNVELLDKTFAQAQDVFRQAMKSPSVILHVLLPQNREQYEKS VIGPLNIFGNNDGASRTKAAPPARGKPGLKAVHLTRASSPEGEEPASPQQSKSPRVPRLG RKPSSPSLSPLMGFGSKKNAKKIKIDLKKGPEGLGFTVVTRDSSIHGPGPIFVKNILPKG AAVKDGRLQSGDRILEVNGRDVTGRTQEELVAMLRSTKQGETVSLVIARQEGSFLPRELD GRLRMNDQLIAVNGETLLGKSNHEAMETLRRSMSMEGNIRGMIQLVILRRPERPLEELSE CGALSRPGFENCQEALSTSRRNDSSILYPFGTYSPQDKRKDLLLPSDGWAENEVPPSPPP HPALEWGLEDFSHSSGVDSTGYFPDQHVNFRTVTPVRQPELINLKASKSMDLVPDEGKVQ SLADRRSDSPGKDFGPTLGLKKSSSLESLQTAVAEVRKNDLPFHRPRPHMVRGRGCNESF RAAIDKSYDGPEEADADGLSDKSSRSGHTALNCESAPQGNPELDNVENKAKNIKKTKEKE KKKGKGKLKVKEKKLKEEHEDAERKMKRKGFGAMLRFGKKKDDKVGKAEQKGAQKSGHPE EEELERMKEERERIGAKHQELREKQARGLVDYATAVTGPVHDMDDDEMDPNYARVNHFRE PCASANVFRSPSPLRAGPLAYPRDGRPLSPDHLEGLYAKVNKPYHPPALADSGRPMAGTT DRIQKLRKEYYQARREGFLLYEDENTRARPSDHDLRWVSGKGPDGSTHNLRFEGMERQYA SLPRGGSADPVDYLTASPRGRYNDRELPYYPGPHPVHAPRGSYPRPPDLRATDLRYPQYY PPPPAHQHKGPFRQDVPPSPPQHQRVPVYQEMGRAGPRGSSPDQYPYRNQDPRQKNPMTA AV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3429
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
