Gene:
Gene Symbol:
Mib2
Gene Name:
mindbomb homolog 2 (Drosophila)
Original Clone ID:
FLH481625.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295378
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2766nts
Open reading frame: 35 to 2800
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACCTGGACCCCCATGCAGGTGT
GCAGGTGGGCATGCGTGTGGTACGCGGAATGGACTGGAAATGGGGCCAGCAGGATGGAGG
TGAGGGCGGTGTGGGCACCGTGGTGGAGCTTGGCCGCCATGGCAGCCCCTCGACCCCCGA
CCGTACAGTTGTCGTTCAGTGGGACCAGGGCACACGTACCAACTATCGAGCTGGCTACCA
AGGTGCCCATGACCTGTTGCTCTATGACAACGCCCAAATCGGTATCCGCCACCCCAATAT
CATCTGTGACTGCTGCAAGAAACATGGGCTTCGGGGCATGCGTTGGAAGTGCCGCGTCTG
CTTTGACTATGACCTCTGCACACAGTGCTACATGCACAACAAGCATGACCTGACCCATGC
CTTCGAGCGCTATGAGACATCTCACTCACGCCCGGTTACGCTGAGCCCCCGACAGGGCCT
CCCTCGGATCCCACTGAGGGGCATCTTTCAAGGAGCTAAGGTGGTACGAGGCCCTGACTG
GGAATGGGGCTCACAAGATGGAGGGGAAGGCAAGACAGGTCGTGTGGTGGACATCCGTGG
CTGGGATGTGGAGACAGGCCGAAGTGTGGCCAGTGTGACGTGGGCAGATGGAACCACGAA
CGTGTACCGAGTGGGCCACAAGGGCAAGGTGGATCTCAGATGTGTTGGTGAGGCAGCTGG
CGGCTTTTACTACAAGGAGCACCTCCCAAAGCTTGGCAAGCCAGCAGAGCTGCAGCGCAG
GGTGAGTGCTGATGGCCAGCCCTTCCAGCGTGGGGACAAGGTCAAGTGTCTGCTGGACAC
AGATGTCCTAAGGGATATGCAAGAAGGCCATGGTGGCTGGAACCCTAGGATGGCAGAGCA
CAACAGCTTCTGGGTGGGTGATGTGGTCCGGGTCATCGGTGACCTTGACACTGTGAAGCG
ACTTCAGGCCGGACATGGTGAATGGACCGACGACATGGCTCCCGCCCTGGGCCGAGTGGG
GAAAGTGGTGAAGGTGTTTGGAGATGGAAACTTGCGTGTGGCGGTTGGCGGTCAGCGGTG
GACCTTCAGCCCCTCTTGCCTAGTGGCCTACCGGCCTGAGGAAGACGCCAACCTGGATGT
GGCTGAGCGTGCCAGAGAGAACAAAAGCTCACTGAGTGTGGCCCTGGACAAGCTTCGGAC
CCAGAAGAGTGACCCAGAGCACCCGGGGAGGCTGGTAGTAGAGGCCGCACTAGGAAATGT
AGCCCGGGCTCTAGATCTTCTGCGGAGGCATCCAGAGCAGGTGGACACCAAGAACCAGGG
TAGAACTGCCCTGCAGGTGGCTGCTTACCTGGGCCAGGTAGAGCTGGTGCGGCTGCTGCT
GCAGGCAAGAGCAAGTATGGACCTGCCGGATGATGAGGGCAACACTGTGCTGCACTACAC
AGCCATGGGGAACCAGCCTGAGGCCACAAGGGTGCTCCTGAGTGCAGGGTGTGCAGTGGA
TGCTCGGAATGGCACACGCAGCACAGCCCTACATGTGGCTGTACAGAGGGGCTTCCTAGA
GGTGGTAAAGATCCTGTGTGAACGTGGTTGTGATGTCAACTTGCCGGATGCGCATGCAGA
CACACCCCTGCATTCTGCCATCTCTGCTGGTGCTGGTGCCAGCAGCATTGTTGAAGTCCT
CACCGAGGTCCCTGGCATCGATGTCACTGCTACCAATAGCCAGGGCTTCACACTGCTACA
CCATGCATCCCTCAAGGGCCATGTGCTAGCAGTCAGGAAGATTCTGGCAAGGGCCCGGCA
GCTGGTGGATGCCAAGAAGGAGGATGGCTTTACCGCACTGCATCTGGCAGCCCTCAACAA
CCACCGGGAGGTGGCCCAGGTCCTAATCCGAGAGGGTCGCTGTGATGTGAATGTGCGGAA
CCGGAAGCTTCAGTCTCCACTTCATCTGGCTGTACAGCAGGCCCACCTGGGGCTGGTACC
GCTGCTAGTGGACGCAGGCTGCAGTGTCAACACGGAGGACGAGGAGGGTGACACGGCCCT
GCATGTGGCACTACAGCGTCACCAGCTGTTGCCCCTTGTGGCTGACAGGGCTGGGGGAGA
CCCAGGGCCCTTGCAGCTGCTGTCAAGGCTACAGGCCTCAGGCCTCCCAGGCTGCACAGA
GTTGACTGTAGGCGCTGCAGTGGCCTGCTTCCTGGCGTTGGAGGGTGCTGATGTGAGCTA
CGCAAACCACCGCGGCCGGAGCCCACTGGACCTGGCCACAGAGGGCCGAGTGCTCAAGGC
CTTGCAGGGCTGTGCCCAGCGCTTCAGGGAGCGACAGGCAGGTGGCGGTGGGGGTGTGCC
CCCGGGCCCCCGGCATGTGCTGAGTACTCCTAACACCGTGACGAACCTGCACGTGTCTGG
CACGGCAGGGCCAGAAGCTGCCGAGTGCCTGGTGTGCTCGGAGCTGGCATTGCTAATTCT
GTTCTCACCGTGTCAGCACCGCACAGTGTGTGAGGAGTGCGCTCGAAGGATGAAGAAGTG
TATCAGGTGCCAGGTGGTCATCAGCAAGAAGCTCCGCCCAGACGGCTCAGAGGTGGTAAA
TGCCATCCAGGTGCCCGGCCCGCCTCGGCAACTGGTGGAGGAGCTACAGAGCCGCTACAG
GCAGATGGAGGAGCGCATCACCTGCCCCATCTGCATCGACAGCCACATCCGCCTGGTGTT
CCAGTGCGGCCATGGAGCGTGCGCGCCCTGTGGCGCCGCGCTTAACGCCTGCCCCATCTG
CCGCCAGCCCATCCGCGACCGCATTCAGATCTTCGTGTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGT RTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYM HNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGK TGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKL GKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRV IGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYR PEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHP EQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRV LLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGA GASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFT ALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNT EDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFL ALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPN TVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKL RPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCG AALNACPICRQPIRDRIQIFV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3598
Start on reference sequence
833
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
