Gene:
Gene Symbol:
Zc3h7b
Gene Name:
zinc finger CCCH type containing 7B
Original Clone ID:
FLH481628.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295381
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2949nts
Open reading frame: 35 to 2983
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGAGGCAGAAACGGAAGGCGGA
CATCGAGAAGGGGCTGCAGTTCATCCAGTCAACACTACCCCTGAAGCAAGAGGAGTATGA
GGCTTTCCTGCTCAAGCTTGTGCAGAACCTGTTTGCCGAGGGCAACGACCTGTTCCGGGA
GAAGGACTACAAGCAGGCTCTGGTGCAGTACATGGAAGGGCTGAACGTGGCTGACTACGC
CGCCTCTGACCAGGTGGCTCTACCCCGGGAGCTGCTGTGCAAACTGCATGTCAACAGGGC
AGCCTGCTACTTCACCATGGGCCTGTATGAGAAGGCGCTGGAGGACAGTGAGAAGGCTTT
GGGCCTGGATGGCGAGAGTATCCGGGCACTGTTCCGCAAGGCGCGCGCCCTCAACGAGCT
GGGGCGCCACAAAGAGGCCTATGAATGCAGCAGCCGGTGCTCCCTCGCGCTTCCCCACGA
TGAAAATGTCACCCAGCTGGGCCAGGAGCTGGCTCAGAAGCTTGGACTGCGGGTACGGAA
AGCCTATAAGAGACCCCAGGAATTGGAAACGTTTTCTCTGCTTAGTAATGGCGCTCCAGC
TGGCTTAACAGATCAGGGAACTTCTAACGGATTGGGATCTATAGATGACATCGAAACAGA
CTGCTACGTGGATCTCCGAGGCTCCCCAGCCCTGCTCCCCTCCACTCCCACGATGCCCCT
GTTCCCTCATGTTCTGGACCTCCTGGCCCCACTGGATGGCAGCAGCAGGACACTCCCCGG
CACCGAGGGCCTAGATGACTTCTCAGATGGGGATGTGTTTGGTCCAGAACTGGACACCCT
CCTGGACTCTCTGTCTCTGGTCCAGGGTGGCCTGCCTGGCAGTGGTATGCCTAGTGAGCT
GCCTCAGCTGATCCCCGTGTTTCCTGGTGGGACCCCACTCTTGCCACCTGTGGTGGGTGG
CTCCATCCCCGTGTCCAGCCCACTGCCTCCTGCCTCCTTTGGCCTTGTCATGGACCCCTC
CAGGAAGCTGGCTGCTTCTGTGCTGGATGCCCTTGACTCTCCAGGGCCCACGCTGGACCC
CCTGGATCTGTTACCATACTCTGAGGCTCGGCTGGGTGCCCTTGACAGCTTTGGGTCAGC
CCGAGGCTCCCTGGACAAGCCGGACTCCTTCCTAGAAGAGACCAGCTCGCAGGACCACCG
ACCCCCAAGCAGTACTCAGAAGCCAGCCCCCTCGCCGGAGCCCTCAATGCCCAACACCAC
CCTGCTCATCAAGAACCCTCTGGCTGCCACCCACGAGTTCAAGCAGGCCTGCCAGTTGTG
CTACCCCAAAACAGGCCCCAGGGCTGGCGACTATACCTACCGTGAGGGCCTCGAGCACAA
GTGCAGAAGGGACGTCCTCCTCGGCCGCCTCCGGAGTTCCGAGAACCAGATCTGGAAGCG
CATCCGGCCGCGGCCCACCAAAACCAGCTTTGTGGGCTCCTACTACCTGTGCAAAGACAT
GATTAACAAGCAAGACTGTAAGTACGGGGATAACTGCACCTTCGCCTACCATCAGGAGGA
GATCGATGTGTGGACAGAGGAGCGGAAGGGCACACTCAACCGGGACCTGCTCTTTGACCC
TCTAGGGGGTGTCAAGCGTGGCAGCCTCACCATTGCCAAGCTCCTGAAGGAGCACCAGGG
CATCTTCACCTTCCTCTGTGAGATCTGCTTCGACAGCAAGCCCCGGATCATCAGCAAAGG
GACCAAGGACTCTCCATCTGTCTGCTCCAACCTGGCTGCCAAGCACAGCTTTTACAACAA
CAAGTGCCTGGTGCACATCGTCCGCTCCACCTCTCTCAAGTACTCCAAGATCCGTCAGTT
CCAGGAGCACTTCCAGTTTGACGTGTGCCGCCACGAGGTGCGCTATGGTTGCCTGCGCGA
GGACAGCTGCCACTTCGCCCATAGTTTCATTGAGCTCAAGGTCTGGTTGCTGCAGCAGTA
CTCAGGAATGACCCATGAAGACATCGTTCAGGAATCCAAGAAATACTGGCAACAGATGGA
AGCCCACGCAGGGAAGGCCAGCAGCGGCTTGGGTGCCCCAAGGACACACGGACCCAGCAC
TTTTGACCTGCAGATGAAGTTTGTGTGCGGTCAGTGTTGGAGGAATGGGCAGGTGGTAGA
GCCGGACAAGGACCTCAAGTACTGCAGTGCCAAGGCCCGGCATTGCTGGACCAAGGAGAG
GCGGGTCCTTCTGGTGATGTCCAAGGCCAAGAGGAAATGGGTGTCCGTGAGGCCGCTGCC
ATCCATTCGAAACTTCCCACAGCAATATGACCTATGTATTCATGCCCAGAACGGCCGCAA
ATGCCAGTATGTGGGGAACTGCTCCTTCGCACACAGCCCTGAGGAGAGGGACATGTGGAC
CTTCATGAAGGAGAACAAGATCCTGGATATGCAACAGACCTATGACATGTGGCTGAAGAA
ACACAACCCAGGGAAGCCGGGAGAAGGGACCCCCATCAGCTCTCGGGAAGGAGAGAAGCA
GATCCAGATGCCCACAGACTATGCTGACATCATGATGGGCTACCACTGCTGGCTCTGCGG
CAAGAACAGCAACAGCAAGAAACAGTGGCAGCAGCACATTCAGTCTGAGAAGCACAAGGA
GAAGGTCTTCACTTCTGACAGCGACGCCAGCGGCTGGGCCTACCGCTTCCCCATGGGCGA
GTTCCGACTCTGTGACAGGCTCCAGAAGGGCAAGGCCTGCCCAGACGGAGACAAGTGCCG
TTGTGCCCATGGACAAGAGGAACTCAACGAGTGGCTGGACCGGCGTGAGGTGCTGAAGCA
AAAGCTTGCCAAGGCCCGCAAGGACATGCTTCTGTGTCCACGGGATGATGACTTCGGCAA
ATACAACTTCCTGCTGCAAGAGGACGGGGACACCACTGGTGCCACCCCAGATGCCCCTGT
TGCTGCAACTGCTACTGCTGCCACCACCACCACTGGGGAGTAGGGCCTCATGGGCCCAGC
TTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MERQKRKADIEKGLQFIQSTLPLKQEEYEAFLLKLVQNLFAEGNDLFREKDYKQALVQYM EGLNVADYAASDQVALPRELLCKLHVNRAACYFTMGLYEKALEDSEKALGLDGESIRALF RKARALNELGRHKEAYECSSRCSLALPHDENVTQLGQELAQKLGLRVRKAYKRPQELETF SLLSNGAPAGLTDQGTSNGLGSIDDIETDCYVDLRGSPALLPSTPTMPLFPHVLDLLAPL DGSSRTLPGTEGLDDFSDGDVFGPELDTLLDSLSLVQGGLPGSGMPSELPQLIPVFPGGT PLLPPVVGGSIPVSSPLPPASFGLVMDPSRKLAASVLDALDSPGPTLDPLDLLPYSEARL GALDSFGSARGSLDKPDSFLEETSSQDHRPPSSTQKPAPSPEPSMPNTTLLIKNPLAATH EFKQACQLCYPKTGPRAGDYTYREGLEHKCRRDVLLGRLRSSENQIWKRIRPRPTKTSFV GSYYLCKDMINKQDCKYGDNCTFAYHQEEIDVWTEERKGTLNRDLLFDPLGGVKRGSLTI AKLLKEHQGIFTFLCEICFDSKPRIISKGTKDSPSVCSNLAAKHSFYNNKCLVHIVRSTS LKYSKIRQFQEHFQFDVCRHEVRYGCLREDSCHFAHSFIELKVWLLQQYSGMTHEDIVQE SKKYWQQMEAHAGKASSGLGAPRTHGPSTFDLQMKFVCGQCWRNGQVVEPDKDLKYCSAK ARHCWTKERRVLLVMSKAKRKWVSVRPLPSIRNFPQQYDLCIHAQNGRKCQYVGNCSFAH SPEERDMWTFMKENKILDMQQTYDMWLKKHNPGKPGEGTPISSREGEKQIQMPTDYADIM MGYHCWLCGKNSNSKKQWQQHIQSEKHKEKVFTSDSDASGWAYRFPMGEFRLCDRLQKGK ACPDGDKCRCAHGQEELNEWLDRREVLKQKLAKARKDMLLCPRDDDFGKYNFLLQEDGDT TGATPDAPVAATATAATTTTGE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3454
Start on reference sequence
506
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
