Gene:
Gene Symbol:
Abi3bp
Gene Name:
ABI gene family, member 3 (NESH) binding protein
Original Clone ID:
FLH481629.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295382
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3240nts
Open reading frame: 35 to 3274
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCTCTCCAGCCTGGGGTGTCTACT
TCTCTGTGGAAGTATTGCACTTGCTCTGGGAAATGCACAGAAATTGCCAAAAGGTAAAAA
GCCAAGCTTGAAAGTGCACATCAATACCACCAGCGACTCCATCCTCTTGAAGTTCCTGCG
TCCAAATGCAAACGTAAAGCTGGAAGGTTTTCTCCTGGGATATGGCAGCAATGTGTCTCC
AAACCAGTACTTCCCTCTTCCCACAGAAGGGAAATTCACAGAGGCTGTAGTTGATGCAGA
GCCGAAGTATCTGATAGTCGTACGACCTGCTCCTCCTCCCAGCCAGAAGAAGTCATGTTC
AGGGAAATCTCGCCCTCGCAAACCCCTCCAGCTGGTGGTGGGTACGTTGACACCGAGTTC
TGTCTTCCTGTCCTGGGGATTCCTCATCAACCCACACCATGACTGGACATTGCCAAGCCA
CTGTCCCAGTGACAGATTTTATACAATTCGATATAGAGAAAAAGATAAAGAAAAGAAATG
GATTTTTCAACTCTGCCCAGCTACTGAAACAATTGTGGAAAATCTAAAGCCCAACACAGT
TTATGAATTTGGTGTGAAAGACAATATAGAAGGTGGAATTTGGAGTAAGATTTTCAATCA
TAAGACTATTGTTGGAAGTAAAAACAAAGTAAACGGGAAAATCCAAAGCACTTATGACCA
GGTCCACTCAGTGCCAGTCCCGAGGAAGCTAATCCCACTAACGATCATTAAGCAAGTGAT
TCAGAATGTTACTCACAGGGCTTCAACCAAATCCCCAGACAAGACTCCTTTTGGGGGAAC
AATATTAGTTCATCTGATTATTCCGGGTCTTAATGAATCCACTGTAAAACTTCCCACATC
CATAATGCTTGAGATTTCCGATGCTCTCAAGGCACAATTAGCTAAGAATGAAACATTGGC
TTTACCAGCAGAATCTAAAACACCAGAGGTCGAAAAATTGGCAGGGCAGCCAGTAACAGT
GACTCCGGAATCAGTTTCAAGAAGCACTAAGCCCACCCTGTCTAGTGCTTTAGACACTGC
AGAGACAGCACTGGTCCTCAGTGAGAAGACGTCAGAAACAGCCCGCTCTGTTCTAATACC
TGAGTTTGAATTGCCCTTGAGCACTTTAGCTCCAAAAAGGTTCCCAGAGTTTCCTGAGGC
AAAAACAGCCTTCCCATTGGAGAAACCTAGGGGTTCCTGGGCTTCAAGTGAGGAGCCATG
GGTGGTACCTGGAGCTAAAACATCTGAAGATTCCAGAGTCGTACAGCCTCAAACTGCAAC
TTATGATGTTATCTCAAGCTCAACAACTTCTGATGAGACTGAAATAGAGATTCACACAGC
AACAAGAGATCCTATTCTGGACTCTGTCCCACCTAAAACTTCTAGAACTGCTGAACAGCC
AAGGGCAACACTGGCTCCAATCGAAGCGTTATTTGAATCCCGAAATGTGGAAATCTTCAC
CAGTCCTGAAGTGCGACCTACAACGGCTGCTCCTCAGCAAACTACATCTATCCCTTCCAC
ACCCAAACGACAATCAACACCCAAACCGCCAAGAGTCAAGCCTGCACCCAGGCAGACAAC
CAGCATGCCTCCCAAGCTCAAAACACCACACTCAAGGATGCCAGCAAAAGAGCCAGTTCC
TAAGGAACCCCTGCATACGACTTCAAAGCCAAAAATGCCACCAAGTCCTGAAGTGGCAGA
CACTACATCTGCACCTTTAGAGACACGGGGCATTCCTCTCATACCTGTGATTTCACCAAG
ACCTAGTCAAGAGGAACTACAAACCGCCATGGAAGAAACAGACCAGTCTACCCAAGAGCT
CTTCACGACTAAGATTCCACGAACAACTGAATTGGCAAAGACAACTCAGGCACCACACAG
ATTGCATACAGCTCCTGTGAGGCCCAGAATACCTGGCAGGCCACATGGCAGGCCTGCTCT
GAACAAAACAACTACAAGGCCTGATAAAACCAAACCCAGAGGGACGTCCCATAAAAACGG
AGTAGGAACAGGAACCAAGCAAGCACCTAAGCCACCAAGTCCTGGGAGAAATGCGTCAGT
GGATTCACATGCCACAAGAAAACCAGGCTCAGTCTCAGGGACGCGTCGTCCACCCATACC
ACATAGGCATTCGTCCACTAGACCTGTGTCTCCAGAGAGAAGGCCTTTACCCCCGAATAA
TGTCACCGGGAAGCCAGGACGTGCGGGAATTGTTTCCTCCAGCCGGGTAACGTCCCCACC
CCTGAAGGCAACACTCCACCCTATCGGAACAGCCACAGCGAGACCAGGGGCAGAACAAAA
GGAACCAACTGCTCCTGCTTCAGAAGAAGAGTTTGGTACTACAACTGACTTCAGTTCAAG
TCCCACCAAAGAAACTGATCCCCTTGGGAAGCCCAGGTTCATAGGTCCCCATGTGCGATA
TATCCCAAAGCCTGAAAACAAGCCCTGTTCCATCACTGACTCAGTCAGACGGTTCCCTAC
AGAGGAAGCCACGGAGGGGAATGCCACCAGCCCACCACAGAACCCACCCACCAACCTCAC
TGTGGTCACTGTCGAAGGCTGCCCCTCATTTGTCATCTTGGACTGGGAAAAACCTCTAAA
TGACACTGTCACTGAATATGAAGTCATATCCAGAGAAAATGGGTCATTCAGTGGGAAGAA
CAAGTCCATTCAAATTACCAATCAAACGTTCTCTACAGTAGAAAACCTGAAACCAGATAC
AAGCTATGAATTCCAGGTGAAACCCAAAAATCCACTTGGTGAGGGCCCCGCCAGCAACAC
GGTAGCGTTCAGTACTGAATCAGCTGACCCAAGAGTGAGTGAGCCAATTTCTGCAGGAAG
AGATGCCATCTGGACTGAAAGACCTTTTAACTCAGACTCTTACTCAGAATGCAAAGGCAA
ACAGTATGTTAAAAGGACGTGGTATAAAAAATTTGTCGGAGTGCAGCTATGTAACTCTCT
CAGATACAAGATTTATTTGAGCGATTCCCTCACAGGCAAGTTTTACAACATAGGAGATCA
GAGGGGCCACGGAGAAGATCACTGCCAGTTTGTGGATTCATTTTTAGATGGTCGCACAGG
ACAGCAACTCACCTCTGAACAGTTACCCACCAAAGAAGGCTATTTCAGAGCTGTTCGTCA
GGAACCTGTCCAATTTGGAGAAATAGGTGGTCATACCCAAATCAATTATGTCCAGTGGTA
TGAATGTGGGACCACAATACCTGGAAAATGGTAGGGCCTCATGGGCCCAGCTTTCTTGTA
C
Protein sequence predicted based on nucleotide sequence
MLSSLGCLLLCGSIALALGNAQKLPKGKKPSLKVHINTTSDSILLKFLRPNANVKLEGFL LGYGSNVSPNQYFPLPTEGKFTEAVVDAEPKYLIVVRPAPPPSQKKSCSGKSRPRKPLQL VVGTLTPSSVFLSWGFLINPHHDWTLPSHCPSDRFYTIRYREKDKEKKWIFQLCPATETI VENLKPNTVYEFGVKDNIEGGIWSKIFNHKTIVGSKNKVNGKIQSTYDQVHSVPVPRKLI PLTIIKQVIQNVTHRASTKSPDKTPFGGTILVHLIIPGLNESTVKLPTSIMLEISDALKA QLAKNETLALPAESKTPEVEKLAGQPVTVTPESVSRSTKPTLSSALDTAETALVLSEKTS ETARSVLIPEFELPLSTLAPKRFPEFPEAKTAFPLEKPRGSWASSEEPWVVPGAKTSEDS RVVQPQTATYDVISSSTTSDETEIEIHTATRDPILDSVPPKTSRTAEQPRATLAPIEALF ESRNVEIFTSPEVRPTTAAPQQTTSIPSTPKRQSTPKPPRVKPAPRQTTSMPPKLKTPHS RMPAKEPVPKEPLHTTSKPKMPPSPEVADTTSAPLETRGIPLIPVISPRPSQEELQTAME ETDQSTQELFTTKIPRTTELAKTTQAPHRLHTAPVRPRIPGRPHGRPALNKTTTRPDKTK PRGTSHKNGVGTGTKQAPKPPSPGRNASVDSHATRKPGSVSGTRRPPIPHRHSSTRPVSP ERRPLPPNNVTGKPGRAGIVSSSRVTSPPLKATLHPIGTATARPGAEQKEPTAPASEEEF GTTTDFSSSPTKETDPLGKPRFIGPHVRYIPKPENKPCSITDSVRRFPTEEATEGNATSP PQNPPTNLTVVTVEGCPSFVILDWEKPLNDTVTEYEVISRENGSFSGKNKSIQITNQTFS TVENLKPDTSYEFQVKPKNPLGEGPASNTVAFSTESADPRVSEPISAGRDAIWTERPFNS DSYSECKGKQYVKRTWYKKFVGVQLCNSLRYKIYLSDSLTGKFYNIGDQRGHGEDHCQFV DSFLDGRTGQQLTSEQLPTKEGYFRAVRQEPVQFGEIGGHTQINYVQWYECGTTIPGKW*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
15
End on reference sequence
3254
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
