Gene:
Gene Symbol:
Pcdhga1
Gene Name:
protocadherin gamma subfamily A, 1
Original Clone ID:
FLH481632.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295385
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2796nts
Open reading frame: 35 to 2830
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCGATTCCAGAGAAGTTAACCGG
CTGGAGCCTGCTGGTGCTGGTGTGTCTTTCTCTGGAGCTACTGCTGGAAGCTGGGGCTGG
AAACATCCGATACTCTGTGCCAGAAGAAACCGACAAAGGCTCCTTCGTGGGCAGCATCGC
CAAGGACCTGGGACTGGAAACCCGGGAGCTGATGGAGCGCGGGATCCGCATCGTTTCCAG
AGGTAGGTCGCAGCTTTTCTCCCTGAACCCGCGAAGCGGCAGCTTGGTCACCGCAGGCAG
GATAGACCGGGAAGAGCTGTGCGCTCAGAGCACGCCATGTGTCGTCAGCTTTAACATCCT
TATGGAGGATGAAATGAAGCTTCTCCCTATCGAAGTAGAGATAATTGATATTAATGACAA
CACTCCCCAATTCCAGTTAGAAGAGCTAGAATTAAAAATGAGTGAAATAACGACTCCAGG
TACCAGGATCCCTCTGCCTCTCGGGCAAGACCTTGATGTGGGGATAAACTCACTTCAGAG
CTACCAGCTAAGCGCCAACCCTCACTTCTCCCTGGATGTGCAACAGGGACCTGAAGGACC
ACAACAGCCAGAGATGGTACTGCAGAGACCTCTAGACAGAGAAAAAGATGCTGTCCACTA
CCTTGTCCTCACTGCCTCGGATGGCGGCAGCCCAATACATTCAGGGACTCTGCAAATTCA
TGTGCAGGTGGTAGATGTGAACGACAACCCACCAGCATTTACTAAGGCAGAGTATCACGT
GAGCGTCCCTGAGAATGTCCCTTTAGGCACTCGGCTACTCAAAGTGAATGCAACTGACCC
GGATGAGGGAGCTAATGGCAGAGTAACCTACTCCTTTCACAAGGTAGACCACAGTGTGGT
CCGGAAATTTCAGTTGGATGCTTACACGGGAGAACTATCAAATAAAGAACCACTAGATTT
TGAAGAATACAAAGTTTATCCAATGGAAATTCAAGCTCAGGATGGTGCTGGACTCATGGC
CAGAGCTAAGGTGCTGGTCACTGTTTTGGATGTTAATGATAATGCCCCGGAAGTTGGAAT
CACGTCTGTCACTAACACAGTACCAGAAAACTTTCCTCCTGGGACCACAATTGCTCTTAT
CAGTGTGCATGATCAAGACGCTGATAACAACGGTCACATCACATGTTCCATCCCTGGCAA
CCTACCCTTTAAACTGGAAAAGCTAGTCGACAATTATTACCGCTTAGTAACAGAAAGAAC
ACTGGACAGAGAACAAAGTTCTAGGCACAACATCACAATAACAGCAACAGATCAGGGAAC
TCCGCCCCTATCTACCCAAGCTCACATCTCACTGCTAGTGACAGATATCAATGACAACCC
TCCAGTTTTTGAACAGGACTCCTATTCTGTCTACATTCCTGAAAACAACCCCAGAGGGGC
TTCCATCTTCTCAGTAAAGGCCCAGGATGCTGACCACAATGAGAATGCACTAGTGACTTA
CGCCCTGGTGGAAGACACTATTCAGGGAGTACCTTTGTCTTCTTACTTCTCCATCAATTC
TGACACTGGAGTTGTCTATGCATTGCAATCCTTTGACTATGAGCAGTTTCGAGATTTGCA
GCTAAGAGTGATGGCCCATGATAGTGGGAACCCACAACTCAGCAGCAATGTATCTCTGAG
TCTGTTCATGATAGATCAGAATGACAATGCCCCAGAGATCCTGTACCCTGTCCTCCCTAC
TGATGGCTCCACTGGTGTGGAGCTAGCACCTCGATCTGCTGAGCCCGGTTACCTAGTGAC
CAAAGTGGTAGCAGTGGACAAAGACTCAGGACAAAACTCTTGGCTGTCCTACCGTCTGCT
CAAGGCCAGTGAACCAGGGCTCTTCTCGGTAGGACTACACACTGGTGAGGTACGCACTGC
TCGGGCCCTGCTGGAGAGAGATGCTCTCAAGCAGAGCCTGGTGGTGGCTGTGCAGGACCA
CGGCCAGCCTCCTCTGTCCACCACAGTCACATTTACCATAGCAGTAGCTGACAGCATCCC
TGACCTCCTGGCTGACCTGGGCAGCATGGGGACCCCTGCCGATCCCCAGGATTCAGACCT
GACACTCTACCTGGTGGTGGCAGTGGCTGTAGTCTCCTGCATCTTCCTAGCCTTTGTAAT
AGTGCTCTTGGTGCTCAAGCTGCGACATTGGTACTCCTCACGCTTGCTCCAGACTGAAGC
AAATGGACTGACTGGCCTGCCTGCCTCCAACTTTGTAGGTGTTGATGGGGTGCATGCATT
TCTACAGACCTATTCCCGTGAGATCTCACTCACTGCTGACTCTCGAAAGAGCCACTTGAT
CTTCCCGCAACCCAACTATGCAGACACACTCATCAGCCACGAGAGCTGTGAGAAACAGGA
ATTTTTGTCAGCACCCCAGTCTCTGCTTGAAGATAAAGAGGAAATATTTTCTCAGCAAGC
CCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCGGCACGAGCGGATCCCA
AAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCTGCAAGC
CATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGC
TGGCACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGA
CTACCGCCAGAACGTGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAA
ACGAGATGGCAAGGCTCCAGCAGGTGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGA
GAAGAAGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MAIPEKLTGWSLLVLVCLSLELLLEAGAGNIRYSVPEETDKGSFVGSIAKDLGLETRELM ERGIRIVSRGRSQLFSLNPRSGSLVTAGRIDREELCAQSTPCVVSFNILMEDEMKLLPIE VEIIDINDNTPQFQLEELELKMSEITTPGTRIPLPLGQDLDVGINSLQSYQLSANPHFSL DVQQGPEGPQQPEMVLQRPLDREKDAVHYLVLTASDGGSPIHSGTLQIHVQVVDVNDNPP AFTKAEYHVSVPENVPLGTRLLKVNATDPDEGANGRVTYSFHKVDHSVVRKFQLDAYTGE LSNKEPLDFEEYKVYPMEIQAQDGAGLMARAKVLVTVLDVNDNAPEVGITSVTNTVPENF PPGTTIALISVHDQDADNNGHITCSIPGNLPFKLEKLVDNYYRLVTERTLDREQSSRHNI TITATDQGTPPLSTQAHISLLVTDINDNPPVFEQDSYSVYIPENNPRGASIFSVKAQDAD HNENALVTYALVEDTIQGVPLSSYFSINSDTGVVYALQSFDYEQFRDLQLRVMAHDSGNP QLSSNVSLSLFMIDQNDNAPEILYPVLPTDGSTGVELAPRSAEPGYLVTKVVAVDKDSGQ NSWLSYRLLKASEPGLFSVGLHTGEVRTARALLERDALKQSLVVAVQDHGQPPLSTTVTF TIAVADSIPDLLADLGSMGTPADPQDSDLTLYLVVAVAVVSCIFLAFVIVLLVLKLRHWY SSRLLQTEANGLTGLPASNFVGVDGVHAFLQTYSREISLTADSRKSHLIFPQPNYADTLI SHESCEKQEFLSAPQSLLEDKEEIFSQQAPPNTDWRFSQAQRPGTSGSQNGDETGTWPNN QFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSN ATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2796
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
