Gene:
Gene Symbol:
Adnp
Gene Name:
activity-dependent neuroprotective protein
Original Clone ID:
FLH481639.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295392
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3327nts
Open reading frame: 35 to 3361
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTTCCAACTTCCTGTCAACAATCT
TGGCAGTTTAAGAAAAGCCCGGAAAACTGTGAAAAAAATACTTAGTGACATTGGGTTGGA
ATACTGTAAAGAACATATAGAAGATTTTAAACAGTTTGAACCTAATGACTTTTATTTGAA
AAACACTACATGGGAGGATGTAGGACTGTGGGACCCTTCTCTTACGAAAAATCAGGACTA
TCGGACAAAACCTTTTTGCTGCAGTGCTTGTCCGTTTTCCTCAAAATTCTTCTCTGCCTA
CAAAAGTCATTTCCGGAATGTCCATAGTGAAGACTTTGAAAATAGGATTCTCCTTAACTG
CCCTTACTGTACCTTCAATGCAGATAAAAAGACTTTGGAAACACACATTAAAATATTTCA
TGCTCCAAACTCCAGCGCACCAAGTAGCAGCCTCAGCACTTTCAAAGATAAAAACAAAAA
CGATGGCCTTAAACCTAAGCAGGCTGACAATGTAGAGCAAGCCGTGTATTACTGCAAGAA
GTGCACTTACCGAGACCCTCTCTACGAGATCGTCAGGAAGCACATCTACAGGGAACATTT
TCAACACGTGGCAGCACCCTACATAGCAAAAGCAGGAGAAAAATCACTCAATGGTGCAGT
CTCCCTGGGCACAAATGCCCGAGAGGAGTGTAACATCCACTGCAAGCGATGCCTTTTCAT
GCCCAAGTCCTATGAAGCTTTGGTACAGCATGTCATTGAGGACCATGAACGGATAGGCTA
TCAGGTCACTGCCATGATCGGACACACAAATGTTGTAGTTCCCCGAGCCAAGCCCTTGAT
GCTGATAGCTCCCAAACCTCAAGACAAAAAGGGCATGGGACTCCCACCACGAATCAGCTC
CCTTGCTTCTGGAAATGTCCGGTCGTTGCCATCACAGCAGATGGTAAACCGATTGTCAAT
ACCAAAGCCCAACTTAAATTCAACGGGAGTCAACATGATGTCCAATGTTCACCTGCAGCA
AAACAACTATGGAGTCAAATCTGTGGGCCAGAGCTATGGTGTTGGCCAGTCAGTGAGGCT
GGGACTAGGTGGCAATGCTCCAGTTTCCATCCCTCAACAGTCTCAGTCCGTGAAACAGTT
ACTTCCAAGTGGGAATGGGAGGTCTTTTGGGCTAGGTGCTGAGCAGAGGCCCCCAGCAGC
AGCCAGGTACTCCCTGCAGACTGCCAACACCTCTCTACCCCCAGGCCAAGTGAAGTCTCC
CTCTGTGTCTCAGTCACAGGCATCTAGAGTATTAGGTCAGTCCAGTTCTAAACCTCCACC
AGCCGCCACAGGCCCTCCTCCAAGCAACCACTGTGCCACTCAGAAGTGGAAAATCTGTAC
AATCTGTAACGAGCTTTTCCCTGAGAATGTCTATAGCGTTCACTTCGAAAAGGAGCATAA
AGCTGAGAAAGTCCCAGCCGTAGCTAACTACATTATGAAAATACACAATTTTACTAGCAA
ATGCCTCTACTGTAATCGCTATTTGCCTACAGATACCCTACTCAACCATATGTTAATTCA
TGGTCTGTCTTGTCCGTATTGCCGTTCCACCTTCAATGATGTAGAGAAGATGGCAGCACA
CATGCGAATGGTTCATATTGATGAAGAGATGGGGCCTAAAACGGATTCTACTTTGAGCTT
TGATTTGACATTGCAACAGGGCAGTCACACCAACATTCATCTCCTGGTGACCACATACAA
CCTGAGGGATGCCCCGGCTGAATCAGTTGCTTACCATGCCCAAAATAATGCCCCAGTTCC
TCCAAAGCCACAACCAAAAGTTCAGGAAAAAGCAGATGTCCCGGTTAAAAGTTCACCTCA
AGCTGCAGTGCCCTATAAAAAAGATGTTGGGAAGACCCTTTGCCCTCTTTGCTTTTCAAT
ACTAAAAGGACCCATATCTGATGCACTTGCACATCATTTACGAGAAAGACACCAAGTTAT
TCAGACAGTTCATCCGGTTGAGAAAAAGCTAACTTACAAATGTATCCATTGCCTTGGTGT
GTATACTAGCAACATGACAGCCTCAACCATCACTCTGCATCTAGTCCACTGCAGGGGTGT
TGGAAAAACCCAGAATGGCCAGGACAAGACAAACGCACCTTCTCGGCTCAATCAGTCTCC
AGGCCTGGCCCCTGTGAAGCGCACGTATGAGCAGATGGAGTTTCCACTGCTAAAAAAGCG
GAAGCTGGAGGAGGATGCTGATTCCCCTAGCTGCTTTGAAGAGAAGCCAGAAGAGCCTGT
TGTTTTAGCTTTAGACCCCAAGGGTCATGAAGATGATTCTTATGAGGCTAGGAAAAGCTT
TCTCACAAAGTACTTCAACAAACAGCCCTATCCCACCAGGAGAGAAATTGAGAAGTTAGC
TGCCAGTCTATGGCTATGGAAGAGTGACATTGCCTCCCATTTCAGTAACAAGAGGAAGAA
GTGTGTCCGCGACTGTGAAAAGTACAAGCCTGGTGTGCTGCTAGGTTTTAACATGAAAGA
ATTAAATAAAGTCAAACACGAGATGGATTTTGATGCTGAGTGGCTGTTTGAAAATCACGA
TGAGAAAGACTCAAGAGTCAATGCTAGCAAGACTGTTGACAAAAAGCATAACCTTGGGAA
AGAAGATGATAGCTTCTCAGATAGTTTTGAACATTTGGAAGAAGAATCCAATGGAAGCGG
GAGTCCTTTTGACCCTGTCTTTGAAGTTGAGCCTAAAATTCCCAGTGATAATTTAGAGGA
GCCTGTACCGAAGGTTATTCCGGAAGGTGCTTTGGAATCTGAGAAGCTAGACCAAAAAGA
GGAGGAGGAGGAGGAGGAGGAGGAGGATGGTTCAAAATATGAAACTATCCATTTGACTGA
GGAACCAGCCAAATTAATGCATGATGCCTCTGATAGTGAGGTAGACCAAGATGATGTAGT
TGAGTGGAAAGATGGTGCTTCACCATCTGAGAGTGGGCCTGGTTCCCAACAAATCTCAGA
CTTTGAGGATAATACATGTGAAATGAAACCAGGAACCTGGTCTGATGAGTCTTCCCAGAG
TGAAGATGCAAGGAGCAGTAAGCCAGCTGCCAAAAAAAAGGCTACAGTGCAAGATGACAC
AGAGCAGTTAAAATGGAAGAATAGTTCCTATGGAAAAGTTGAAGGGTTTTGGTCCAAGGA
CCAGTCACAGTGGGAAAATGCATCTGAGAATGCAGAGCGCTTACCAAACCCACAGATTGA
GTGGCAGAATAGCACAATTGACAGTGAGGACGGGGAGCAGTTTGACAGCATGACTGACGG
AGTTGCTGATCCCATGCATGGCAGCTTAACTGGAGTGAAGCTGAGCAGCCAGCAAGCCTA
GGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MFQLPVNNLGSLRKARKTVKKILSDIGLEYCKEHIEDFKQFEPNDFYLKNTTWEDVGLWD PSLTKNQDYRTKPFCCSACPFSSKFFSAYKSHFRNVHSEDFENRILLNCPYCTFNADKKT LETHIKIFHAPNSSAPSSSLSTFKDKNKNDGLKPKQADNVEQAVYYCKKCTYRDPLYEIV RKHIYREHFQHVAAPYIAKAGEKSLNGAVSLGTNAREECNIHCKRCLFMPKSYEALVQHV IEDHERIGYQVTAMIGHTNVVVPRAKPLMLIAPKPQDKKGMGLPPRISSLASGNVRSLPS QQMVNRLSIPKPNLNSTGVNMMSNVHLQQNNYGVKSVGQSYGVGQSVRLGLGGNAPVSIP QQSQSVKQLLPSGNGRSFGLGAEQRPPAAARYSLQTANTSLPPGQVKSPSVSQSQASRVL GQSSSKPPPAATGPPPSNHCATQKWKICTICNELFPENVYSVHFEKEHKAEKVPAVANYI MKIHNFTSKCLYCNRYLPTDTLLNHMLIHGLSCPYCRSTFNDVEKMAAHMRMVHIDEEMG PKTDSTLSFDLTLQQGSHTNIHLLVTTYNLRDAPAESVAYHAQNNAPVPPKPQPKVQEKA DVPVKSSPQAAVPYKKDVGKTLCPLCFSILKGPISDALAHHLRERHQVIQTVHPVEKKLT YKCIHCLGVYTSNMTASTITLHLVHCRGVGKTQNGQDKTNAPSRLNQSPGLAPVKRTYEQ MEFPLLKKRKLEEDADSPSCFEEKPEEPVVLALDPKGHEDDSYEARKSFLTKYFNKQPYP TRREIEKLAASLWLWKSDIASHFSNKRKKCVRDCEKYKPGVLLGFNMKELNKVKHEMDFD AEWLFENHDEKDSRVNASKTVDKKHNLGKEDDSFSDSFEHLEEESNGSGSPFDPVFEVEP KIPSDNLEEPVPKVIPEGALESEKLDQKEEEEEEEEEDGSKYETIHLTEEPAKLMHDASD SEVDQDDVVEWKDGASPSESGPGSQQISDFEDNTCEMKPGTWSDESSQSEDARSSKPAAK KKATVQDDTEQLKWKNSSYGKVEGFWSKDQSQWENASENAERLPNPQIEWQNSTIDSEDG EQFDSMTDGVADPMHGSLTGVKLSSQQA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3821
Start on reference sequence
495
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
