Gene:
Gene Symbol:
Zfp369
Gene Name:
zinc finger protein 369
Original Clone ID:
FLH481648.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295401
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2538nts
Open reading frame: 35 to 2572
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACCCCGGAGAAGGACGATCAGC
ATTCTGCCTCCTACCGCTAGAGAACACTGAGGACAGTATGGCTTCCACGCTTCCAACAAC
CTGGCCCCATGAATCTGTGAAGTTTGAAGATGTATCACTGAGATTTACCGAGGAAGAGTG
GGCACTGCTAGATCGCCAACAGAAGTGCCTGTACAGGGAAATCATGATGGAGAACCTCAA
TAACATGATTTCAGTAGAGCATCACTTTTCCAAGGCAAATGTGATGCCTCAGTTAGAAGA
AGTAGAAGACTGCTGGCCAATGCAGAGAGAAATTCCTCAAGATACTCTTCCAGAATGCTC
TGGGCATTCTCTGGACCCTGAAATGAATTCCTTTCCTGCTGAGAGTCCCCTGATGAAGAT
TAAGGTTGTTGAGGTCCTTACATTGAACAAGGATATGGCTGGTCCCCGGAATGCCCTGAT
TCAATCCCTCTATCCAGAAAGTGGGGAAGACTTGAACCCAGGTAACCTCAAGCCAGCTCA
ACAGCCAAGCAAGCATTTGACTGACACTGAAGCTTCGCGACAGAAGTTCCGACATTTCCA
GTATGAAGAGTCAGCTGGTCCCCAGAAGTCTGTGTCCCAGCTTCGTAAGCTGTGTCACCA
GTGGCTGCAGCCCAGTACACGTTCAAAGAAGCAGATCCTAGAGTTGCTGGTGCTCGAGCA
GTTCCTGAATGCATTGCCTGAGAAGCTTCGGGTATGGGTAGAGTCGCAGCACCCAGAAGA
CTGCAAAGCAGTGGTGGCTTTGTTGGAGAATATGACCTCAGTGTCTAAGGACGATGCTTG
GCTTGCCTGTTCTAGTGAAGCCACTGATGAACTAAAGGAGAAAAGGAAGGATGTAGCCAC
CTTACCAGTTACTGTACCACCTGAGGCGCCGGTGACTTTCCAGGATGTGGCTGTAGACTT
CAGTCAGGAGGAGTGGCAGCTACTAGGTCCAATGCAAAGGACCAAGTATCATGATGTGAT
GCTAGAGACCTTGGGCAACTTGGTGTCTGTGGGGTGGGAGCCTACGTTGGGAAACAGAGA
GTTAACTCCAGACTCTCCCATACCTGTGGTAAAGCCAATTCATGACCCAAACCCAAAAGA
TCTCTCGAGGAATCGTACCCAGTCTACCCTCTTTGAAAGTATCTCACCAGATGAGGTCCA
GGGAAGGCACACCATAGAATCAAATCCAGTGGGGCTTCTACAGGAAAAAGGCCACCCTCA
GCAGAAGCTCTCTGAAAGCTCCAAGTCTCAGAACCAAACTTGTATTGACAAAAGCCAGTG
CTCACTCAATGAAGTTCTTCCTAGAAATCATGTAAAAGTTAAGCAGAAGGGCACGGGAAA
GGGGAAAGGCAGGACAAACACCATTTCCATGACAGGAGGTTTACGCATAAGAAATCAGCA
GAAGGACTCCGTGGAGTGGCGAGACAGGAGTGGCAGCACTCCTGTGACACATGGCTCCTC
TATAAAAAACCAACAATTGGGTTCTGAGCAGGGAAAAACTCAGACCAGCAGAGATCCCAT
TACACTTGCTGTGCCTGCAGAATTTTACCAGGAAGCCACTGGCTCTGAAGAGGGTAGGTT
TAGGAACAGTAGCAATGCCATGGCAGCTGATGCACCTCCAAAGATTCACCAGAAAGGCCC
TGAATGGCATAAAGCTGGGAAAAGTAACAATTCCATGTTACAAGGTTCATCTGCACAAAA
TCACCAGATGGGGTCTAGGGCAGGAAGAGCCAGGGACAACAGCATCTTAACACATGTAAA
AATTCACCAGAAAGGCTATAAAGAAGGCAAAATCCAAGGAAACAATAATTACTTGAAACA
TGTAAAACCTCACCAGAAGGGTTCTAAAGAGGAAAGACTCAGGGAACTTAGCACTTGTCA
AAAACATGTCCCCTATGTAAAACAACACCTAAAGACCTCAGGAAGAGGAAAAGGCAGGAA
AATCAATGCTTCCATAAAATGTGGTCCCTATATAAAAACTTACTATAGAGGTTCTGATGT
GGGAAGGCTCAGGAGAGCCAACAATTGTAGGAAAGCCATCAGCCGGCATGCTCAGCAGAT
ATCCTTTATAAAAATTCACAAGGGGAGCCAAATTTGCCAATGCAGTGAATGTGGTAAAAT
GTTCCGGAATGCTAGGTATTTCTCTGTCCATAAGAAGATCCACACAGGAGAGCGGCCTTA
CATGTGTATGTCCTGTGGGAAAGCATTTGTTCAGAGCTCCTCCCTCACACAGCATCTTAG
AATTCACAGTGGAGAGAGACCCTTTGAGTGTTCAGAGTGTGGGAGAACTTTCAGTGACCG
CTCAGCTGCCTCTCAGCACCTGAGAACTCATACTGGAGCTAAGCCCTACCAATGTCAGCA
CTGTGGGAAAGCTTTCCGCCAGAGTTCCCACCTCACCAGGCATGTGAGAATTCATACTGG
GGAGCGACCTTATGTGTGTACCAAGTGTGGGAAGGCATTCACACAGAGCTCAAACCTTAT
TGGGCATCAGAAAACACACAGAAAGAAGTTCAAGAAGCAGCCTAAATTGTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDPGEGRSAFCLLPLENTEDSMASTLPTTWPHESVKFEDVSLRFTEEEWALLDRQQKCLY REIMMENLNNMISVEHHFSKANVMPQLEEVEDCWPMQREIPQDTLPECSGHSLDPEMNSF PAESPLMKIKVVEVLTLNKDMAGPRNALIQSLYPESGEDLNPGNLKPAQQPSKHLTDTEA SRQKFRHFQYEESAGPQKSVSQLRKLCHQWLQPSTRSKKQILELLVLEQFLNALPEKLRV WVESQHPEDCKAVVALLENMTSVSKDDAWLACSSEATDELKEKRKDVATLPVTVPPEAPV TFQDVAVDFSQEEWQLLGPMQRTKYHDVMLETLGNLVSVGWEPTLGNRELTPDSPIPVVK PIHDPNPKDLSRNRTQSTLFESISPDEVQGRHTIESNPVGLLQEKGHPQQKLSESSKSQN QTCIDKSQCSLNEVLPRNHVKVKQKGTGKGKGRTNTISMTGGLRIRNQQKDSVEWRDRSG STPVTHGSSIKNQQLGSEQGKTQTSRDPITLAVPAEFYQEATGSEEGRFRNSSNAMAADA PPKIHQKGPEWHKAGKSNNSMLQGSSAQNHQMGSRAGRARDNSILTHVKIHQKGYKEGKI QGNNNYLKHVKPHQKGSKEERLRELSTCQKHVPYVKQHLKTSGRGKGRKINASIKCGPYI KTYYRGSDVGRLRRANNCRKAISRHAQQISFIKIHKGSQICQCSECGKMFRNARYFSVHK KIHTGERPYMCMSCGKAFVQSSSLTQHLRIHSGERPFECSECGRTFSDRSAASQHLRTHT GAKPYQCQHCGKAFRQSSHLTRHVRIHTGERPYVCTKCGKAFTQSSNLIGHQKTHRKKFK KQPKL*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
119
End on reference sequence
2656
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
