Gene:
Gene Symbol:
Adamts6
Gene Name:
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 6
Original Clone ID:
FLH481650.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295403
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3354nts
Open reading frame: 35 to 3388
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAAATTTCGTGGAAAACGTTGAC
TTGGATTCTGAGCCTCATCATGGCCTCATCGGAATTTTATAGTGACCACAGACTTTCATA
TAGTTCTCAAGAGGAATTCCTGACTTATCTTGAACACTACCAGCTAACTATTCCAATAAG
AGTTGACCAAAATGGAGCTTTTCTCAGCTTTACTGTGAAAAATGATAAACACTCAAGAAG
AAGACGGAGTATGGACCTTCTTGATCCACAGCAGGCAGTGTCTAAATTATTTTTTAAACT
TTCAGCCTATGGCAAGCACTTTCACCTAAACTTGACTCTCAATACAAATTTTGTGTCCAA
ACATTTTACAGTAGAGTATTGGGGGAAAGATGGACCCCAGTGGAAACATGATTTTTTAGA
CAACTGTCACTACACAGGATACTTGCAAGATCAACATAGTACAACTAAAGTGGCTTTAAG
CAACTGTGTTGGATTGCATGGTATTATTGCTACAGAAGATGAAGAATATTTTATTGAGCC
TCTAAAGAATACTACAGAGGATTCCAAACATTTTAGTTATGAAAATGGCCATCCTCATGT
TATTTACAAAAAGTCTACTCTTCAGCAACAACATATCTATGATCATTCTCATTGTGGGGT
TTCAGATCTCACAGGAAGTGGCAAACCTTGGTGGCTGAATAAGACATCATCTTTTCCTTC
TTTACCACCAATTAATGACACACATATTCATCACAGAAAGAGAAGATCTGTGAGCACTGA
GAGATTTGTGGAGACTTTAGTAGTGGCAGACAAAATGATGGTGGGCTACCATGGCCGTAA
AGACATTGAACATTATATTTTAAGTGTGATGAATATTGTTGCCAAACTTTATCGTGACTC
CAGTCTAGGAAACGTTGTGAATATTATAGTGGCTCGCTTAATTGTTCTCACTGAAGATCA
GCCAAACTTGGAGATAAACCATCATGCAGACAAGTCCCTCGATAGCTTCTGTAAATGGCA
GAAATCCATTCTCTCCCACCAAAGTGATGGAAACACCATTCCAGAAAATGGGATTGCCCA
CCACGATAATGCAGTTCTTATTACTAGATATGATATCTGCACGTATAAAAACAAGCCCTG
TGGAACACTGGGCTTAGCCTCTGTGGCTGGAATGTGTGAGCCTGAAAGGAGCTGCAGCAT
TAATGAAGACATTGGCCTAGGCTCGGCTTTTACCATCGCACATGAGATTGGTCACAATTT
TGGTATGAACCATGATGGAATTGGAAATTCTTGTGGGACGAAAGGTCATGAGGCAGCAAA
ACTTATGGCAGCTCATATTACTGCAAATACCAATCCCTTCTCCTGGTCTGCCTGCAGCAG
AGACTACATCACCAGCTTTCTAGATTCAGGCCGTGGTACTTGCCTTGATAATGAGCCTCC
AAAGCGTGACTTTCTTTATCCAGCTGTGGCCCCAGGTCAGGTGTATGATGCTGATGAACA
ATGTCGTTTCCAGTATGGAGCAACATCCCGCCAATGCAAATATGGGGAAGTGTGTAGAGA
GCTCTGGTGCCTCAGCAAAAGCAACCGCTGTGTCACCAACAGTATTCCAGCTGCTGAGGG
GACACTGTGTCAGACTGGGAATATTGAGAAAGGGTGGTGTTATCAGGGAGATTGTGTTCC
TTTTGGCACATGGCCCCAGAGTATTGATGGGGGCTGGGGTTCCTGGTCACTGTGGGGCGA
GTGCAGTAGGACCTGCGGAGGAGGTGTCTCCTCGTCTCTAAGACACTGTGACAGTCCAGC
ACCTTCAGGAGGTGGGAAATATTGCCTTGGTGAAAGGAAACGCTACCGCTCTTGCAACAC
AGATCCATGCCCTTTGGGTTCCCGAGATTTTCGAGAAAAACAGTGTGCAGACTTTGACAG
TATGCCTTTCCGGGGAAAGCATTATAACTGGAAACCCTATACTGGAGGTGGGGTAAAACC
TTGTGCATTAAATTGCCTGGCTGAAGGCTATAATTTCTACACAGAGCGAGCTCCTGCTGT
GATAGATGGGACACAGTGCAATGCAGACTCACTGGATATCTGTATCAATGGAGAATGCAA
GCACGTAGGTTGTGATAATATTTTGGGATCTGATGCTAGGGAAGATAGGTGCCGAGTCTG
TGGTGGGGATGGAAGCACCTGTGATGCCATTGAAGGGTTCTTCAATGACTCATTGCCCAG
AGGAGGTTATATGGAAGTGGTTCAGATACCAAGAGGCTCTGTTCATATTGAAGTAAGAGA
AGTTTCCATGTCAAAAAATTACATTGCTTTAAAATCTGAAGGGGATGATTACTACATTAA
TGGTGCCTGGACTATTGACTGGCCTAGGAAATTTGATGTTTCGGGGACAGCTTTTCACTA
CAAGAGACCGACAGATGAACCAGAATCCTTGGAAGCTCTAGGACCCACCTCTGAGAACCT
CATTGTAATGGTTCTACTCCAAGAACAGAATTTGGGAATTAGGTACAAGTTCAATGTTCC
CATCGTTCGTACTGGCAGTGGAGATAATGAAGTTGGCTTCATGTGGACTTATCAGCCCTG
GGCGGAGTGCTCTGCTACTTGTGCTGGAGGTGTCCAAAGACAAGAAGTGGTCTGTAAAAG
ATTGGATGACAACTCCATTGTCCAGAACAATTTCTGTGATCCTGACAGTAAGCCACCTGA
AAATCAAAGAGCCTGCAACACTGAGCCTTGCCCACCTGAATGGTTCATTGGAGACTGGTT
GGAGTGCAGCAAGACTTGTGATGGTGGGATGCGCACAAGGGCCGTGCTCTGCATCAGGAA
AGTTGGACCTTCTGAAGAGGAAACACTGGACTACGGCGACTGCTTAACACACCGGCCTGT
GGAGAAAGAGTCCTGCAACAACCAGTCATGTCCACCTCAGTGGGTAGCTTTGGACTGGTC
CGAATGTACTCCTAAATGTGGTTCAGGATTCAAGCATCGGATTGTTCTGTGCAAGAGCAG
TGACCTTTCTAAAACATTCCCAGCTGCACAATGTCCAGAGGAGAGCAAGCCTCCTGCTCG
CATCCGCTGCAGTCTGGGCCGCTGCCCTCCTCCCCGCTGGGTGACAGGAGACTGGGGCCA
GTGCTCTGCTCAGTGTGGTCTTGGACAACAAATGAGAACTGTCCAGTGCCTTTCCTATAC
TGGACAGGCGTCTGTTGACTGTCCAGAAACTGTCCGGCCTCCATCCATGCAGCAGTGTGA
CAGCAAATGTGATAGCACCCCTCTCTCCAGTACTGAAGAGTGCAAAGATGTGAACAAAGT
GGCTTATTGCCCACTGGTGCTGAAGTTCAAGTTCTGCAGTCGAGCATACTTCAGACAGAT
GTGTTGTAAGACCTGCCAAGGACACTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MEISWKTLTWILSLIMASSEFYSDHRLSYSSQEEFLTYLEHYQLTIPIRVDQNGAFLSFT VKNDKHSRRRRSMDLLDPQQAVSKLFFKLSAYGKHFHLNLTLNTNFVSKHFTVEYWGKDG PQWKHDFLDNCHYTGYLQDQHSTTKVALSNCVGLHGIIATEDEEYFIEPLKNTTEDSKHF SYENGHPHVIYKKSTLQQQHIYDHSHCGVSDLTGSGKPWWLNKTSSFPSLPPINDTHIHH RKRRSVSTERFVETLVVADKMMVGYHGRKDIEHYILSVMNIVAKLYRDSSLGNVVNIIVA RLIVLTEDQPNLEINHHADKSLDSFCKWQKSILSHQSDGNTIPENGIAHHDNAVLITRYD ICTYKNKPCGTLGLASVAGMCEPERSCSINEDIGLGSAFTIAHEIGHNFGMNHDGIGNSC GTKGHEAAKLMAAHITANTNPFSWSACSRDYITSFLDSGRGTCLDNEPPKRDFLYPAVAP GQVYDADEQCRFQYGATSRQCKYGEVCRELWCLSKSNRCVTNSIPAAEGTLCQTGNIEKG WCYQGDCVPFGTWPQSIDGGWGSWSLWGECSRTCGGGVSSSLRHCDSPAPSGGGKYCLGE RKRYRSCNTDPCPLGSRDFREKQCADFDSMPFRGKHYNWKPYTGGGVKPCALNCLAEGYN FYTERAPAVIDGTQCNADSLDICINGECKHVGCDNILGSDAREDRCRVCGGDGSTCDAIE GFFNDSLPRGGYMEVVQIPRGSVHIEVREVSMSKNYIALKSEGDDYYINGAWTIDWPRKF DVSGTAFHYKRPTDEPESLEALGPTSENLIVMVLLQEQNLGIRYKFNVPIVRTGSGDNEV GFMWTYQPWAECSATCAGGVQRQEVVCKRLDDNSIVQNNFCDPDSKPPENQRACNTEPCP PEWFIGDWLECSKTCDGGMRTRAVLCIRKVGPSEEETLDYGDCLTHRPVEKESCNNQSCP PQWVALDWSECTPKCGSGFKHRIVLCKSSDLSKTFPAAQCPEESKPPARIRCSLGRCPPP RWVTGDWGQCSAQCGLGQQMRTVQCLSYTGQASVDCPETVRPPSMQQCDSKCDSTPLSST EECKDVNKVAYCPLVLKFKFCSRAYFRQMCCKTCQGH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3763
Start on reference sequence
410
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
