Srgap3 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Srgap3
Gene Name:  SLIT-ROBO Rho GTPase activating protein 3
Original Clone ID:
FLH481655.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295408
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3300nts
Open reading frame: 35 to 3334

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCATCTCAAACTAAGTTCAAGAA
AGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGATCCGCACGCAGCTGGT
GGAGCAGTTCAAATGCCTGGAGCAGCAGTCCGAGTCGCGGCTGCAGCTGCTGCAAGACTT
GCAGGAGTTCTTCCGCAGGAAAGCTGAGATTGAGCTGGAGTATTCCCGAAGCCTGGAGAA
GCTGGCTGAGCGCTTCTCCTCCAAGATTCGCAGCTCCCGGGAGCACCAGTTCAAGAAGGA
TCAATACCTCCTCTCGCCCGTGAACTGTTGGTATCTGGTACTGCATCAGACTCGGCGGGA
GAGCCGAGACCATGCCACCCTCAACGACATCTTCATGAACAATGTCATCGTCCGCCTCTC
CCAGATCAGTGAAGATGTCATCAGACTCTTCAAAAAGAGCAAAGAGATTGGCCTGCAGAT
GCATGAGGAGCTGCTGAAGGTCACCAACGAGCTCTACACAGTCATGAAAACCTACCACAT
GTACCATGCAGAGAGCATCAGTGCAGAAAGCAAGCTGAAGGAGGCTGAGAAGCAGGAGGA
GAAACAGTTTAACAAGTCAGGAGAGCTAAGCATGAACCTGCTCCGGCACGAGGACAGACC
CCAGCGCCGCAGCTCCGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTA
TTCTGAGAACAAGCTCAAGTGCACGAAGGCCCGGAATGATTACCTGCTGAATCTGGCAGC
CACCAACGCAGCTATCAGCAAATACTACATCCACGATGTCTCTGATCTCATCGATTGCTG
TGATTTGGGCTTTCACGCCAGCCTGGCCCGCACTTTTCGGACCTACCTCTCAGCAGAATA
TAACCTGGAGACCTCTCGCCATGAGGGTCTGGACGTCATAGAGAATGCAGTGGACAACCT
GGACTCCCGGAGTGACAAGCACACAGTCATGGATATGTGCAGCCAGGTCTTTTGCCCTCC
ACTCAAGTTTGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGTGCACAGCA
GCCTGTCCAGACAGAGCTGCTCATGCGGTACCACCAGCTGCAGTCCAGGCTGGCCACTCT
CAAGATTGAGAATGAAGAGGTTCGGAAAACCCTGGATGCCACCATGCAGACCTTACAGGA
CATGCTGACCGTGGAAGACTTCGATGTCTCTGACGCCTTCCAACACAGCCGATCTACAGA
GTCCATAAAGTCAGCTGCTTCTGAGACCTACATGAGCAAAATCAACATCGCCAAAAGGAG
AGCCAACCAGCAGGAAACAGAAATGTTTTATTTTACAAAATTTAAAGAATATGTGAATGG
CAGTAACCTCATCACCAAGCTGCAAGCCAAGCATGACTTACTCAAGCAGACCCTGGGTGA
AGGGGAGAGAGCAGAGTGCGGCACGACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAA
AATGAGGAGACCTAGGCCTCTTTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGA
AGCGTTCATTAAGGATTCAGGACAAGCTATACCACTTGTAGTTGAGAGCTGTATTCGATT
CATCAATTTATATGGACTCCAGCAGCAGGGGATCTTCAGAGTTCCAGGGTCTCAGGTTGA
AGTCAATGACATCAAGAATTCCTTTGAGAGAGGTGAAGACCCCCTCGTGGATGACCAAAA
TGAGCGAGATATCAATTCAGTTGCTGGTGTTTTAAAACTGTATTTCCGAGGACTGGAAAA
CCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGAGAACCC
AGCTGACAGGGTGCACCCAATCCAGCAGATCCTCATCACCCTGCCCCGCGTGGTCATTGT
GGTCATGAGATACCTCTTTGCTTTCCTCAATCACCTTTCCCAGTACAGTGATGAGAACAT
GATGGATCCCTACAACTTGGCCATCTGCTTCGGGCCCACCCTCATGCACATCCCCGATGG
GCAGGACCCTGTATCCTGCCAGGCGCATGTCAATGAAGTCATCAAAACTATCATCATCCA
CCATGAAGCCATTTTCCCCAGCCCTCGGGAGCTAGAGGGACCCGTGTACGAAAAATGCAT
GGCTGGAGGGGAAGAGTACTGTGACAGCCCACACAGTGAGCCGGGGACCATTGATGAAGT
TGACCATGACAATGGCACGGAGCCTCACACCAGTGACGAAGAAGTGGAGCAGATTGAGGC
CATCGCCAAGTTTGACTATGTGGGGCGGTCCCCTCGTGAACTATCCTTCAAAAAAGGGGC
CTCACTACTCCTGTATCACCGTGCCTCAGAGGACTGGTGGGAGGGCCGCCACAACGGTGT
AGATGGACTCATCCCTCATCAGTACATAGTTGTACAGGACATGGACGATGCCTTCTCCGA
TAGCCTGAGCCAAAAGGCTGACAGCGAGGCCAGCAGTGGGCCACTGCTGGATGACAAGGC
CTCTTCCAAGAATGACCTCCAGTCCCCCACAGAGCACATCTCTGATTACGGCTTTGGGGG
GGTGATGGGCCGAGTGCGACTACGGTCCGACGGAGCAGCCATCCCCAGGCGCCGAAGTGG
GGGCGACACACACAGCCCACCCCGGGGCTTGGGTCCCAGCATAGACACGCCACCCCGAGC
TGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCCCTCAGCCGGGGACGGATCGAGAGCCC
TGAGAAGAGAAGGATGGCGACTTTCGGGAGCGCTGGCAGTATCAACTACCCTGACAAGAA
GGCGCTGACAGAAGGGCTCTCCATGAGGTCGACTTGCGGTTCCACGCGACACAGCAGCCT
AGGGGACCACAAGTCCCTGGAGGCTGAGGCCCTGGCAGAAGACATCGAGAAGACCATGAG
CACCGCTCTGCATGAGTTGCGGGAACTTGAGAGACAGAACACTGTCAAGCAGGCACCAGA
TGTGGTGCTGGACACCCTGGAGCCCCTGAAGAATCCACCAGGCCCCATCAGCTCAGAGCC
CGCCAGCCCCCTGCACACCATTGTCATCCGAGACCCTGATGCTGCTATGCGCCGAAGCAG
CAGCTCATCGACGGAGATGATGACCACCTTCAAGCCAGCCCTGTCAGCTCGCCTGGCTGG
TGCCCAGCTGCGTCCGCCCCCCATGCGTCCGGTCAGACCTGTGGTTCAGCACCGATCCAG
CAGCAGCAGCAGCTCAGGTGTGGGCAGCCCGGCAGTGACACCCACGGAGAAGATGTTCCC
TAATAGCTCCTCGGACAAGTCTGGCACCATGTAGGGCCTCATGGGCCCAGCTTTCTTGTA
C

Protein sequence predicted based on nucleotide sequence

MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIE
LEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIF
MNNVIVRLSQISEDVIRLFKKSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESK
LKEAEKQEEKQFNKSGELSMNLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKAR
NDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLD
VIENAVDNLDSRSDKHTVMDMCSQVFCPPLKFEFQPHMGDEVCQVSAQQPVQTELLMRYH
QLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYM
SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRP
PCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRFINLYGLQQQGI
FRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDL
ISTIKLENPADRVHPIQQILITLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYNLAICFG
PTLMHIPDGQDPVSCQAHVNEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPH
SEPGTIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYVGRSPRELSFKKGASLLLYHRASED
WWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE
HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIP
LSRGRIESPEKRRMATFGSAGSINYPDKKALTEGLSMRSTCGSTRHSSLGDHKSLEAEAL
AEDIEKTMSTALHELRELERQNTVKQAPDVVLDTLEPLKNPPGPISSEPASPLHTIVIRD
PDAAMRRSSSSSTEMMTTFKPALSARLAGAQLRPPPMRPVRPVVQHRSSSSSSSGVGSPA
VTPTEKMFPNSSSDKSGTM*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4070
Start on reference sequence 771
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable