Gene:
Gene Symbol:
Epha6
Gene Name:
Eph receptor A6
Original Clone ID:
FLH481658.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295411
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3393nts
Open reading frame: 35 to 3427
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCAATTCCCCTCGCCTCCAGCCGC
CAGGAGCTCCCCGGCGGCGCAGGCAGCATCGTCCCCGCAAGCAGCTGCTCCAGCACCTGG
GCAGCCTGGACCCTCGTGCCCTGCGCACCGGGCCTCGCGCGGGGGGCGCCCCGGGACTTC
CCCTGCGGACCGGGTGGAGGAGGAAGAGGAGGAGGAGGAAGAAGAAGAGAGCTTGGTTCA
GGACCCCCACGCTACTAGGAACACCTGGCTGCGCTGCTGCCACTTCTTTTTAAGGAGGAG
AAGAGAACCAACCAGAGCCATGGGGGGCTGCGAAGTCCGGGAATTTCTTTTGCAATTTGG
TTTCTTCTTGCCCCTGCTAACGGCTTGGACCGGCGACTGCAGTCACGTCTCCAACCAAGT
TGTGTTGCTTGATACAACCACAGTGATGGGAGAACTAGGATGGAAAACATATCCATTAAA
TGGGTGGGATGCCATTACTGAAATGGATGAACACAATAGGCCCATACATACATACCAGGT
ATGCAATGTCATGGAACCAAACCAGAACAACTGGCTTCGTACTAACTGGATCTCTCGTGA
TGCTGCACAGAAAATTTATGTGGAAATGAAGTTCACACTGAGGGATTGTAACAGCATCCC
ATGGGTCTTGGGGACTTGTAAAGAAACCTTTAACCTGTATTATATAGAATCCGATGAATC
CCATGGGACAAAATTCAAGCCAAGCCAATATATAAAGATCGACACAATTGCTGCTGATGA
GAGTTTTACTCAGATGGATTTGGGTGACCGCATCCTTAAACTCAACACTGAAATCCGTGA
GGTGGGGCCTATAGAAAGGAAAGGATTTTATTTGGCTTTTCAAGATATTGGAGCATGCAT
CGCTCTGGTCTCAGTCCGAGTTTTCTACAAAAAATGCCCCTTCACCGTGCGGAACTTGGC
TATGTTTCCTGATACCATCCCAAGGGTTGATTCTTCCTCTTTGGTTGAAGTGCGGGGCTC
ATGTGTAAAGAGTGCTGAGGAGCGAGATACTCCTAAACTCTACTGTGGAGCTGATGGGGA
TTGGCTTGTTCCTCTTGGAAGGTGTATCTGCAGTACAGGGTATGAAGAAATCGAGGGTTC
TTGCCATGCTTGCAGACCAGGATTCTACAAAGCATTTGCTGGGAACACAAAATGTTCCAA
ATGCCCTCCACACAGCTCAACCTACGTGGAAGCAACGTCAGTCTGTCATTGTGAAAAGGG
TTACTTCCGGGCAGAAAAAGACCCACCTTCTATGGCATGCACTAGACCACCTTCAGCTCC
TAGAAATGTGGCTTTTAACATCAATGAAACAGCCCTTATTTTGGAATGGAGCCCACCCAG
TGACACAGGAGGGAGAAAAGATCTCACATACAGTGTAATCTGTAAGAAATGTGGTTTAGA
CACTACCCAGTGTGAGGACTGTGGTGGAGGACTCCGCTTCATCCCAAGACACACTGGACT
GATCAACAATTCTGTGGTAGTACTGGACTTTGTGTCTCACGTCAATTATACCTTTGAAAT
AGAAGCCATGAATGGAGTTTCTGAGTTGAGCATCTCTCCCAAGCCATTCACAGCTATTAC
AGTGACTACAGATCACGATGCACCTTCTCTGATTGGTATGATGAGAAAGGACTGGGCATC
TCAGAACAGCCTTGCTCTATCGTGGCAAGCACCTGCATTTTCCAATGGAGCTATTCTGGA
CTATGAGATCAAGTACTACGAGAAAGAGCATGAGCAGCTCACCTATTCCTCCACGAGGTC
CAAGGCCCCAAGCGTCATCGTCACGGGCCTCAAGCCCGCCACCACGTACATATTTCACAT
CCGAGTGAGGACGGCGACAGGCTACAGTGGCTACAGTCAGAAGTTTGAATTTGAAACAGG
AGATGAAACTTCTGACATGGCGGCAGAACAAGGGCAGATTCTGGTCATAGCCACTGCAGC
CGTCGGGGGATTCACTCTCTTAGTCATCCTCACCCTGTTCTTCCTCATCACTGGGAGGTG
TCAATGGTACATAAAGGCCAAAATGAAGTCAGAAGAGAAGAGAAGAACTCACTTACAGAA
CGGCCACCTGCGCTTCCCGGGAATTAAAACATACATTGATCCAGACACCTATGAAGACCC
ATCCCTAGCAGTCCACGAATTTGCAAAAGAGATTGATCCTTCAAGAATTCGCATTGAGAG
AGTGATTGGAGCAGGTGAATTTGGAGAAGTCTGCAGTGGGCGTTTGAAGACACCAGGGAA
AAGGGAGATCCCAGTTGCAATTAAAACATTGAAAGGTGGCCACATGGACCGACAAAGAAG
AGATTTTCTAAGAGAAGCTAGCATCATGGGTCAGTTTGACCACCCAAACATCATTCGCCT
AGAAGGTGTTGTCACTAAAAGATCCTTCCCGGCGATTGGGGTGGAAGCCTTCTGCCCCAG
CTTCCTAAGGGCTGGGTTTTTAAATGGCATCCAAGCGCCACATCCAGTGACTGCAGGAGG
CTCTCTGCCCCCCAGGATTCCTGCAGGTCGGCCAGTAATGATCGTAGTAGAGTATATGGA
GAATGGATCACTGGACTCCTTTTTGCGGAAGCATGATGGCCACTTCACCGTCATCCAGTT
GGTCGGCATGCTGAGGGGCATCGCGTCAGGCATGAAGTATCTTTCTGACATGGGATATGT
TCATCGAGATCTTGCCGCGAGGAACATACTGGTGAACAGCAACTTAGTATGCAAGGTCTC
TGATTTTGGTCTCTCCCGAGTGCTGGAAGATGATCCAGAAGCAGCTTATACAACAACGGG
AGGGAAAATCCCTATACGGTGGACAGCCCCAGAAGCTATTGCTTACAGAAAGTTCTCCTC
AGCCAGTGACGCGTGGAGCTACGGGATTGTCATGTGGGAGGTGATGTCCTATGGAGAGAG
ACCGTACTGGGAAATGTCCAACCAGGATGTTATCTTGTCCATTGAAGAAGGTTACCGACT
TCCTGCTCCGATGGGCTGCCCACCGTCTCTGCACCAGCTGATGCTCCACTGCTGGCAGAA
GGAGAGAAACCACAGGCCAAAATTCACTGACATCGTCAGCTTCCTTGACAAACTGATCCG
CAACCCCAGTGCCCTTCACACGCTGGTGGAGGACATCCTTGTAATGCCAGAATCCCCTGG
TGATGTTCCTGAATATCCATTGTTTGTCACAGTAGGTGACTGGCTGGATTCTATAAAGAT
GGGGCAATACAAGAGTAACTTCATGGCAGCGGGTTTTACAACGTTTGATCTGATTTCACG
AATGAGCATCGATGATATTAGGCGAATTGGAGTCATTCTCATTGGACATCAGAGACGAAT
AGTCAGCAGCATACAAACTTTACGTTTACATATGATGCACATACAGGAAAAAGGATTTCA
TGTATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MQFPSPPAARSSPAAQAASSPQAAAPAPGQPGPSCPAHRASRGGRPGTSPADRVEEEEEE EEEEESLVQDPHATRNTWLRCCHFFLRRRREPTRAMGGCEVREFLLQFGFFLPLLTAWTG DCSHVSNQVVLLDTTTVMGELGWKTYPLNGWDAITEMDEHNRPIHTYQVCNVMEPNQNNW LRTNWISRDAAQKIYVEMKFTLRDCNSIPWVLGTCKETFNLYYIESDESHGTKFKPSQYI KIDTIAADESFTQMDLGDRILKLNTEIREVGPIERKGFYLAFQDIGACIALVSVRVFYKK CPFTVRNLAMFPDTIPRVDSSSLVEVRGSCVKSAEERDTPKLYCGADGDWLVPLGRCICS TGYEEIEGSCHACRPGFYKAFAGNTKCSKCPPHSSTYVEATSVCHCEKGYFRAEKDPPSM ACTRPPSAPRNVAFNINETALILEWSPPSDTGGRKDLTYSVICKKCGLDTTQCEDCGGGL RFIPRHTGLINNSVVVLDFVSHVNYTFEIEAMNGVSELSISPKPFTAITVTTDHDAPSLI GMMRKDWASQNSLALSWQAPAFSNGAILDYEIKYYEKEHEQLTYSSTRSKAPSVIVTGLK PATTYIFHIRVRTATGYSGYSQKFEFETGDETSDMAAEQGQILVIATAAVGGFTLLVILT LFFLITGRCQWYIKAKMKSEEKRRTHLQNGHLRFPGIKTYIDPDTYEDPSLAVHEFAKEI DPSRIRIERVIGAGEFGEVCSGRLKTPGKREIPVAIKTLKGGHMDRQRRDFLREASIMGQ FDHPNIIRLEGVVTKRSFPAIGVEAFCPSFLRAGFLNGIQAPHPVTAGGSLPPRIPAGRP VMIVVEYMENGSLDSFLRKHDGHFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILV NSNLVCKVSDFGLSRVLEDDPEAAYTTTGGKIPIRWTAPEAIAYRKFSSASDAWSYGIVM WEVMSYGERPYWEMSNQDVILSIEEGYRLPAPMGCPPSLHQLMLHCWQKERNHRPKFTDI VSFLDKLIRNPSALHTLVEDILVMPESPGDVPEYPLFVTVGDWLDSIKMGQYKSNFMAAG FTTFDLISRMSIDDIRRIGVILIGHQRRIVSSIQTLRLHMMHIQEKGFHV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
284
End on reference sequence
3676
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
