Gene:
Gene Symbol:
Recql4
Gene Name:
RecQ protein-like 4
Original Clone ID:
FLH481659.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295412
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3651nts
Open reading frame: 35 to 3685
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGCGGCTCGCGACCGTTCGCGC
GCGGCTACAGGAGTGGGAACGCGCTTTTGCACGGCTGCACGGGCGACGGCCAGCGAAGGG
GGATGTGGAGGCGGCACCTGAAGAGACCCGCGCGCTCTACCGTGAGTACCGTAACCTAAA
GCAGGCGGTGCGTCAGGCTGACGACAGACATCGTGTCCTAGAGCAATCACTTGCCGAGGC
AGCTGAGGAGGCACAGGAGCCAAGCTGCTGGGGTCCCCACCTGAGTCGAGCTGCAACGCA
GAATACGCAGTCTATGCCAAAACAGAGCCTACTGAGTTCTGTACAAGACTATGGGAAGAG
GCTCAAAGCCAATCTGAAAAACACAACACAGACTGGACCAACCCAGAGCAGAAAACTCCA
GCTTCAGAAGAGATCCTTGTCCACAGTTCCTGCCCCAAGGCCACCAGGCTCAAAGACTGA
ATCCCCCTGTCCAGACGAAGCTGACGATGCACTTCCTCGGGTTCCTGAGCCCCGGCCGAG
GCTGGGCCAGCTCCAGCAGCTCCGATCATCCCTCAGCCGGAGGTTGACTTCCCTAGACCC
TGGTTGGTTAGAGAGGTGTCACAACAGAGTTTCAGATCTTCTAGAGGTTCCGGGTGCTTG
TGGGCTTGACCTGAGTGCAGAGGAGTCACAGCCTCAGATGTCAGGCAAGGTGAACATCGC
TGATCCTGACATCCAGTCAGAAGTATCTGTACAGAGCCCAGAGGCCATAGCCCAACAGCC
AGCCCAGGTTTTGTCACAGAGCCCCAAATCCATCAACAGTAAAGGCAGGAAGCGGAAGTG
GAATGAGAAGGGGGAGGACTTTGCACAAGACCAGCCCAGCAGCGGAGCAGGACCCCTGTC
TGAGGGAGCCAGGGCTACAGTACATGGGCAAGACCCTCCAGGAGAACCCACACAAGTGAA
TGTCCCTCAGCCATGCAATTCCTCAAACCAGGCCAGGACAGAGAAGGCTAAGGGCACAAC
CCACCTCCATGCCTCTCCTCGACCAGCTTCCCTAGACAGAGGGAACTATATTCGACTCAA
CATGAAAAACAAACGCTTTGTACGAGTTGGGGCCAATCGGGGCAGGCTTCTCCGTAAGCA
GGTATGGAAGCAAAAGTGGAAGAAGAAACAAGCTGCGTTTGGGGGAAGTGGACCCAGGGC
CACAGACAAGGACACTTGTTTCCGGTGTGGGCAGTTTGGTCACTGGGCATCCCAGTGTTC
CCAACCAGGCCCCACCCTGACCGTCCAAGAGGAAGGTGACAGGGATGACAAACAGCCCAT
TTCCACCTTGGAAGAAGTAGCACAGAGGACAGGCACTGCTTCCTGTCACCACTCTGGTGA
GGAAACACAGCCTGCTGCGCCAGAGCTACAGGTGCCTCATTGCCCCACCCCAATGTCACC
CCTCTACCCACCGGGACCTTTGGGACAAGTAGCAGAAACCCCTGCTGAAGTATTCCAGGC
CCTAGAGCGGCTAGGGTACCGAGCCTTCCGCCCTGGGCAAGAGCGTGCAATCATGCGGAT
TCTTTCTGGCATCTCTACTCTGTTAGTGTTGCCCACGGGTGCTGGAAAGTCTCTGTGCTA
CCAGCTTCCTGCACTGCTCTATGCCCAGCGAAGCCCCTGCCTCACACTCGTGGTCTCGCC
TCTCCTGTCACTCATGGATGACCAGGTGTCCGATCTGCCTTCATGTCTGAAGGCAGCCTG
CCTCCACTCAGGAATGACCAAGAAACAACGAGAGTCTGTCTTGAAGAAGGTACGGGCAGC
CCAGGTGCACGTGCTGATCGTGTCCCCAGAGGCCTTGGTGGGGTGCGGGGCTAGGGGTCC
CGGCAGCCTCCCCCAGGCCGCTCAGCTGCCTCCAATTGCCTTCGCCTGCATTGATGAGGT
CCACTGCCTCTCTCAGTGGTCACATAACTTCCGGCCCTGCTACCTACGTGTTTGCAAAGT
TCTCCGGGAGCATATGGGGGTGCGCTGCTTCTTGGGTCTCACAGCCACAGCCACACGAAG
CACTGCTCGAGATGTGGCTCAGCACCTTGGCATAGCTGGCGAGTTTGAGCTCAGCGGGTC
AGCCAACATCCCTGCCAATCTGCACCTCTCCGTGTCCATGGATAGAGACTCAGACCAGGC
TCTGGTGACATTGCTGCAAGGGGACCGTTTTCGTACCCTGGATTCAGTTATCATTTACTG
CACTCGGCGAAAGGATACAGAACGGGTGGCTGCACTCCTCCGCACCTGCCTGTCCATGGT
GGGCGACTCAAGGCCAAGAGGCTGTGGCCCCGAGGCTATAGCTGAAGCCTACCATGCTGG
CATGAGCAGCCAGGAACGGCGACGAGTACAACAGGCCTTCATGCGGGGCCACCTGCGCAT
GGTAGTGGCCACGGTAGCATTTGGGATGGGACTGGACCGTCCAGATGTTCGGGCTGTGCT
GCACCTGGGACTGCCTCCAAGCTTCGAGAGCTACGTGCAAGCTATCGGCCGTGCAGGGCG
TGATGGGAAGCCTGCCCATTGCCACCTATTCATGCACCCCCAGGGTGAAGACCTTTGGGA
ACTGCGCAGACATGCCCACGCTGACAGCACTGACTTCCTAGCTGTGAAGAGGCTGGTGCA
GCGTGTGTTCCCACCCTGCACCTGCAGCCAGAGACCTGTTTCCAAGTCCTCACCTGAGGA
AGTCAAAGAGCACAGTGGCCAACAAACATACCCTGTACTGGGCCAGGCCTGCCTGGGCCA
TGAGCGGGCACTCCCAGTGCAGTCTACAGTACAGGCTCTGGACATGACAGAGGAGGCTAT
TGAGACTCTGCTGTGCTATTTGGAACTACACCCTCGGCACTGGTTGGAGCTGCTGCCCTG
GACCTACGCCCAGTGCCATCTGCATTGCCTTGGCGGCAGTGCCCAGCTGCAAGCTCTGGC
CCACAGGTGTCCCCCTTTGGCTGCATGCCAGGCCAAGTGGCCACCTAAAGACACAAGTCA
GGGCAGGAGCTCCTTAGAGTTTGGTGTGGTGGAACTGGCAGACTCGATGGGCTGGAAGTT
GGCCTCTGTACGGCAGGCTCTCCACCAGCTGAAGTGGGACCCAGAGCCAAAGAAAGGCGC
AGCACAGGGCACCGGAGTGCTTGTGAAGTTCAGCGAGTTGGCCTTTCACCTGCACAGTCG
CGGGGACCTGACAGATGAGGAAAAGGACCAGATCTGTGACTTTCTGTACAACCGTGTGCA
GGCTCGTGAACACAAGGCCCTGGCCCACCTACACCAAATGTCCAAGGCCTTTCGAAGTGT
GGCCTTTCCCAGTTGTGGACCCTGTTTAGAGCAGTCTAATGAGGAGCACAGCAATCAGGT
GAAGACCCTGGTCAGCTACTACTTTGAGGAAGAGGAGGAGGAGGAAGAAACTATGACGGA
CACTCAGGGTCCAAAACCTGGGCAGACTCAGCTTCAGGACTGGGAGGACCAAATACGCCG
GGATGTCCGCCAGCTCCTGTCCCTGAGGCCAGAAGAAAGGTTTTCAGGAAGGGCTGTGGC
CCGCATCTTCCATGGCATTGCGAGTCCATGCTACCCAGCCCAGGTGTATGGGCTGGACCG
GCGCTTCTGGAGGAAGTACCTACACCTGGACTTTCATGCCCTGATGCACCTAGCTACAGA
AGAGCTCCTGCTGAGAGGCCGATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MERLATVRARLQEWERAFARLHGRRPAKGDVEAAPEETRALYREYRNLKQAVRQADDRHR VLEQSLAEAAEEAQEPSCWGPHLSRAATQNTQSMPKQSLLSSVQDYGKRLKANLKNTTQT GPTQSRKLQLQKRSLSTVPAPRPPGSKTESPCPDEADDALPRVPEPRPRLGQLQQLRSSL SRRLTSLDPGWLERCHNRVSDLLEVPGACGLDLSAEESQPQMSGKVNIADPDIQSEVSVQ SPEAIAQQPAQVLSQSPKSINSKGRKRKWNEKGEDFAQDQPSSGAGPLSEGARATVHGQD PPGEPTQVNVPQPCNSSNQARTEKAKGTTHLHASPRPASLDRGNYIRLNMKNKRFVRVGA NRGRLLRKQVWKQKWKKKQAAFGGSGPRATDKDTCFRCGQFGHWASQCSQPGPTLTVQEE GDRDDKQPISTLEEVAQRTGTASCHHSGEETQPAAPELQVPHCPTPMSPLYPPGPLGQVA ETPAEVFQALERLGYRAFRPGQERAIMRILSGISTLLVLPTGAGKSLCYQLPALLYAQRS PCLTLVVSPLLSLMDDQVSDLPSCLKAACLHSGMTKKQRESVLKKVRAAQVHVLIVSPEA LVGCGARGPGSLPQAAQLPPIAFACIDEVHCLSQWSHNFRPCYLRVCKVLREHMGVRCFL GLTATATRSTARDVAQHLGIAGEFELSGSANIPANLHLSVSMDRDSDQALVTLLQGDRFR TLDSVIIYCTRRKDTERVAALLRTCLSMVGDSRPRGCGPEAIAEAYHAGMSSQERRRVQQ AFMRGHLRMVVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAIGRAGRDGKPAHCHLFM HPQGEDLWELRRHAHADSTDFLAVKRLVQRVFPPCTCSQRPVSKSSPEEVKEHSGQQTYP VLGQACLGHERALPVQSTVQALDMTEEAIETLLCYLELHPRHWLELLPWTYAQCHLHCLG GSAQLQALAHRCPPLAACQAKWPPKDTSQGRSSLEFGVVELADSMGWKLASVRQALHQLK WDPEPKKGAAQGTGVLVKFSELAFHLHSRGDLTDEEKDQICDFLYNRVQAREHKALAHLH QMSKAFRSVAFPSCGPCLEQSNEEHSNQVKTLVSYYFEEEEEEEETMTDTQGPKPGQTQL QDWEDQIRRDVRQLLSLRPEERFSGRAVARIFHGIASPCYPAQVYGLDRRFWRKYLHLDF HALMHLATEELLLRGR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3651
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
