Robo1 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Robo1
Gene Name:  roundabout homolog 1 (Drosophila)
Original Clone ID:
FLH481663.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295416
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4839nts
Open reading frame: 35 to 4873

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGATCGCGGAGCCTGCTCGCTTTTA
CCTCTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGAAGATTTTCCACC
CCGCATTGTTGAACACCCTTCAGACCTGATCGTCTCCAAAGGAGAACCTGCCACTCTGAA
CTGTAAAGCCGAAGGCCGCCCCACCCCGACGATAGAATGGTACAAAGGTGGGGAAAGAGT
GGAGACGGACAAAGATGACCCGCGCTCACACCGGATGCTGCTGCCAAGCGGGTCTTTATT
TTTCTTACGCATAGTCCATGGACGGAAGAGTAGGCCCGACGAAGGGGTCTACATCTGTGT
GGCTAGGAATTACCTCGGAGAGGCTGTGAGCCACAACGCATCGCTGGAGGTAGCTATACT
ACGGGATGACTTCAGACAAAACCCTTCAGATGTCATGGTGGCAGTAGGCGAACCTGCAGT
CATGGAATGCCAGCCTCCACGGGGCCATCCAGAGCCCACCATCTCCTGGAAAAAGGATGG
CTCACCCCTGGATGATAAAGATGAAAGAATCACTATACGAGGAGGAAAACTCATGATCAC
TTATACGCGTAAAAGCGATGCTGGCAAGTATGTTTGCGTTGGCACCAACATGGTGGGGGA
ACGGGAGAGTGAAGTCGCAGAGCTAACCGTTTTAGAGAGACCATCGTTTGTGAAGAGACC
CAGTAATTTGGCAGTGACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCGAGGAGA
CCCTGTGCCTACAGTTCGATGGAGGAAAGATGACGGAGAGCTGCCCAAATCCAGATATGA
AATCCGAGATGATCATACTTTGAAAATTCGGAAGGTGACTGCTGGCGACATGGGATCATA
CACTTGTGTGGCAGAAAATATGGTCGGGAAAGCTGAAGCTTCCGCTACTTTGACAGTTCA
AGAGCCGCCACATTTCGTTGTAAAACCTCGGGACCAGGTGGTTGCCTTGGGAAGAACTGT
GACGTTTCAGTGCGAAGCAACTGGAAATCCTCAACCAGCTATCTTTTGGAGGAGAGAGGG
GAGTCAGAATCTACTTTTCTCCTACCAGCCTCCACAGTCCTCTAGCCGATTTTCAGTTTC
GCAGACAGGAGACCTCACCATTACGAACGTCCAGCGGTCTGATGTCGGCTACTACATCTG
TCAGACTTTAAATGTTGCTGGAAGTATCATCACAAAGGCCTATTTGGAAGTTACTGATGT
GATTGCAGACCGGCCTCCCCCAGTCATTCGGCAAGGTCCTGTGAATCAGACTGTAGCAGT
GGATGGCACTTTAATCCTCAGCTGTGTGGCCACAGGCAGTCCAGCACCCACAATTCTGTG
GAGGAAGGATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGAGAGTGG
CGTGCTGCAGATCCGCTACGCTAAGCTGGGTGACACGGGTCGGTACACCTGCACCGCATC
GACCCCCAGCGGTGAGGCCACGTGGAGCGCTTACATCGAAGTCCAGGAATTTGGGGTTCC
AGTTCAACCCCCAAGACCCACCGACCCCAACTTAATCCCCAGCGCCCCCTCAAAGCCCGA
AGTTACAGATGTCAGCAAAAACACGGTCACTTTATCGTGGCAGCCAAACTTGAACTCAGG
AGCAACCCCAACATCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTGGCA
GACGGCGGCAGAAAATGTGAAAACAGAAACATTTGCCATCAAAGGACTCAAACCTAACGC
CATTTACCTTTTCCTGGTGAGGGCAGCAAATGCCTATGGAATTAGTGATCCAAGCCAAAT
ATCAGATCCAGTGAAAACGCAAGATGTCCCACCAACCAGCCAAGGGGTGGACCACAAGCA
GGTCCAAAGAGAACTGGGGAATGTTGTCTTACATCTGCACAACCCGACCATCCTTTCTTC
CTCCTCAGTCGAAGTGCACTGGACGGTGGACCAACAATCTCAATATATTCAAGGCTATAA
AATTCTCTACCGCCCATCTGGAGCCAGCCATGGTGAATCGGAGTGGTTAGTTTTTGAAGT
GAGGACGCCAACGAAAAACAGCGTGGTGATCCCCGATCTCAGAAAAGGCGTCAACTACGA
GATTAAGGCTCGCCCATTTTTTAATGAGTTTCAAGGAGCAGACAGTGAAATCAAATTTGC
CAAAACCTTAGAAGAAGCACCAAGTGCCCCTCCCCGAAGTGTAACTGTATCCAAGAATGA
TGGAAATGGGACTGCAATCCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAAAATGG
AATGGTCCAAGAATATAAGGTTTGGTGTCTCGGTAATGAAACGAAGTATCACATAAACAA
GACAGTCGATGGCTCGACCTTCTCGGTGGTCATCCCCTCTCTGGTTCCTGGGATCCGATA
CAGTGTGGAGGTGGCAGCGAGCACTGGGGCGGGTCCCGGGGTGAAGAGCGAACCTCAGTT
CATCCAGTTAGATTCTCACGGAAACCCCGTGTCTCCCGAGGACCAAGTCAGCCTTGCTCA
GCAGATCTCCGACGTGGTGAGGCAGCCGGCGTTCATTGCAGGCATTGGGGCAGCCTGTTG
GATTATCCTCATGGTCTTCAGCATCTGGCTTTACAGACACCGCAAGAAGAGAAACGGACT
CACCAGCACGTATGCGGGTATCCGGAAAGTCCCGTCCTTTACCTTCACGCCAACAGTAAC
TTATCAAAGAGGAGGCGAAGCCGTCAGCAGTGGAGGGAGGCCGGGACTCCTCAACATTAG
TGAACCTGCCACGCAGCCATGGCTGGCAGACACGTGGCCCAACACAGGCAACAACCACAA
TGACTGCTCCATCAACTGCTGCACGGCCGGCAATGGCAACAGCGACAGCAACCTGACAAC
CTACAGTCGCCCAGCTGATTGTATAGCCAATTATAACAACCAACTGGATAACAAACAAAC
AAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAATCAA
TGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGACGGGCGTTTTGTCAATCCATCAGG
GCAGCCCACTCCCTATGCCACCACTCAACTCATCCAGGCGAACCTCAGCAACAATATGAA
TAATGGCGCCGGGGACTCCAGCGAGAAGCACTGGAAGCCTCCGGGACAGCAGAAACCGGA
AGTGGCACCAATTCAGTATAACATCATGGAGCAAAACAAGCTGAACAAAGATTATCGGGC
AAATGACACGATCCCTCCAACCATCCCATACAACCAGTCCTATGACCAGAACACAGGAGG
GTCTTATAACAGCTCAGATCGGGGCAGTAGTACATCCGGGAGTCAAGGCCACAAGAAAGG
GGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGGCAGACTTGCTGCCCCC
TCCTCCGGCGCATCCTCCCCCACATAGCAACAGTGAAGAATACAACATGTCCGTAGATGA
AAGCTATGATCAAGAAATGCCCTGTCCAGTGCCACCTGCGCGGATGTATTTGCAACAGGA
TGAATTAGAGGAAGAGGAAGATGAAAGAGGCCCCACTCCCCCTGTCCGGGGGGCAGCTTC
TTCTCCAGCTGCTGTGTCCTACAGCCACCAATCCACGGCCACTCTCACCCCTTCTCCCCA
GGAAGAGCTCCAGCCCATGTTACAGGATTGTCCGGAGGACCTCGGGCACATGCCCCACCC
ACCAGACAGGAGGCGGCAGCCCGTGAGTCCTCCTCCACCGCCACGGCCAATTTCCCCACC
TCATACTTACGGCTACATTTCAGGACCCCTTGTCTCAGATATGGATACCGATGCCCCAGA
AGAGGAGGAGGACGAAGCTGACATGGAAGTTGCCAAAATGCAGACCCGAAGGCTGTTGTT
ACGTGGGCTGGAGCAGACCCCAGCATCCAGTGTTGGGGACCTTGAGAGCTCCGTCACTGG
GTCCATGATCAATGGCTGGGGCTCAGCCTCGGAAGAGGACAACATTTCCAGTGGGCGCTC
CAGTGTCAGTTCATCCGATGGCTCCTTTTTCACTGACGCTGATTTTGCCCAGGCAGTTGC
TGCAGCTGCGGAGTATGCGGGCCTGAAAGTGGCTCGCCGCCAAATGCAAGATGCTGCTGG
CCGCCGCCACTTCCATGCCTCTCAGTGCCCAAGGCCCACGAGTCCTGTGTCCACAGACAG
CAACATGAGTGCTGTTGTGATCCAGAAAGCCAGACCCGCCAAGAAGCAGAAACACCAGCC
AGGACATCTGCGCAGGGAAGCCTACGCAGATGATCTTCCACCCCCTCCAGTGCCACCACC
TGCTATAAAATCGCCCACTGTCCAGTCCAAGGCACAGCTGGAGGTACGGCCTGTCATGGT
GCCAAAACTCGCGTCTATAGAAGCAAGGACAGATAGATCGTCAGACAGAAAAGGAGGCAG
TTACAAGGGGAGAGAAGCTCTGGATGGAAGACAAGTCACTGACCTGCGAACAAATCCAAG
TGACCCCAGAGAGGCACAGGAACAGCCAAATGACGGGAAAGGCCGAGGAACAAAGGCAGC
CAAGCGAGACCTTCCACCAGCAAAGACGCACCTCGGCCAAGAGGATATTCTTCCGTACTG
TAGACCTACTTTCCCAACGTCAAACAACCCTAGAGACCCCAGTTCCTCCAGCTCTATGTC
ATCAAGAGGATCAGGGAGCCGACAAAGAGAGCAAGCAAATGTGGGCCGAAGGAATATGGC
AGAAATGCAAGTACTTGGGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGA
AACTGAAAGCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MIAEPARFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTI
EWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSH
NASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERIT
IRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSA
EFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKA
EASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPP
QSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ
GPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGD
TGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTL
SWQPNLNSGATPTSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANA
YGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQ
QSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQ
GADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLG
NETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVS
PEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVP
SFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGN
GNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLK
DGRFVNPSGQPTPYATTQLIQANLSNNMNNGAGDSSEKHWKPPGQQKPEVAPIQYNIMEQ
NKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGG
MNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDELEEEEDERGP
TPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPP
PPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSV
GDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVA
RRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADD
LPPPPVPPPAIKSPTVQSKAQLEVRPVMVPKLASIEARTDRSSDRKGGSYKGREALDGRQ
VTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHLGQEDILPYCRPTFPTSNNPR
DPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 5830
Start on reference sequence 992
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable