Gene:
Gene Symbol:
Whsc1l1
Gene Name:
Wolf-Hirschhorn syndrome candidate 1-like 1 (human)
Original Clone ID:
FLH481670.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295423
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4341nts
Open reading frame: 35 to 4375
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATTTCTCTTTCTCTTTCATGCA
AGGGATCATGGGAAACACAATTCAGCAACCACCTCAACTCATTGACTCGGCCAACATCCG
CCAGGAGGATGCCTTTGATAACCACAGTGACATTGTTGAAGATGGTGGCCCGACACCCTT
TGAAGCTACTTTGCAACAAGGTTTTCAATATCCACCTACAACAGAAGACCTTCCTCCGCT
CACAAATGGCTACCCACCATCAATCAGCTTGTATGAAACTCAAACCAAATACCCGCCATA
TAATCAGTATCCCAATGGGTCAGCCAACGGTTTTGGTGCAGTTAGAAACTTTAGCCCTAC
TGACTATTACCATTCAGAAATTCCAAACACAAGACCACATGAAATTCTGGAAAAACCTTC
TCCTCCTCAGCCACCTCCTCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCTTCGGT
ACCACAAACTGTGATTCCAAAGAAGACAGGCTCACCCGAGATTAAACTAAAAATAACCAA
AACTATCCAGAATGGCAGGGAATTGTTTGAGTCTTCCCTTTGTGGAGACCTCTTAAATGA
AGTACAGGCAAGCGAGCACACAAAGTCTAAGCATGAAAGCAGAAAGGAAAAGAGGAAAAA
GAGCAACAGACACGAGTCATCTCGATCCGAAGAGCGGAGGTCACACAAGATTCCCAAGCT
AGAGCCGGAGGGACAGAATAGACCAAATGAGAGGGTGGACACTGCACCAGAGAAGCCAAG
AGAAGAGCCAGTGCTCAAAGAGGCCATCCCGGTTCAGCCAATACTGTCTTCTGTTCCAAC
AACAGAAACATCCACTGGTGTTAAGTTCCAGGTTGGTGATCTTGTTTGGTCCAAGGTGGG
AACCTACCCTTGGTGGCCTTGTATGGTTTCAAGTGATCCCCAGCTTGAGGTCCATTCCAA
AATTAACACAAGAGGTGCCCGGGAATATCATGTCCAATTTTTTAGCAACCAGCCAGAGAG
GGCATGGGTTCATGAGAAACGGGTACGGGAATACAAAGGTCATGAACAGTATGAAGAGTT
ACTAGCCGAGGCAGCCAAGCAAGCCAGCAATCATTCTGAAAAGCAAAAGATTCGGAAACC
CCGACCTCAGAGAGAACGTGCCCAATGGGACATTGGCATTGCTCATGCAGAGAAAGCATT
GAAAATGACTCGGGAGGAAAGAGTAGAGCAGTACACTTTCATCTACATTGATAAGCAGCC
AGAAGAGGCTTCGTCCCAAGCAAAGAAGAATGTTACCTCTAAGACGGAAGTCAAGAAACC
CCGAAGACCAAGATCTGTGCTGAACAGTCAGCCAGAACAGACCAATGCTGGGGAGGTGGC
CTCCTCACAATCAAGTACTGACCTTCGAAGGCAGAGCCAGAGGCGGCATACTAGCTTGGA
AGAGGAAGAGCCACCTCCTGTTAAAATCGCCTGGAAAACAGCAGCCGCAAGGAAGTCCTT
ACCAGCCTCCATCACAATGCACAAAGGGAGCCTAGATTTGCAGAAGTGTAATATGTCTCC
AGTTGTGAAAATTGAACAAGTGTTTGCTCTCCAGAATGCAACAGGAGATGGGAAGTTTAT
TGATCAGTTTGTTTATTCAACGAAGGGTATTGGTAACAAAACAGAAATAAGTGTCAGGGG
GCAAGACAGGCTTATAATCTCTTCACCAAGTCAGAGAAGTGAAAAGCCGGCTCAGAGCGC
GTCATCTCCGGAGGCAACATCTGGTTCTGCAGGCCCAGTAGAGAAGAAGCAGCAGAGAAG
ATCCATCAGGACTCGATCTGAGTCAGAGAAGTCCGCCGAGGTTGTGCCAAAGAAGAAGAT
CAAAAAGGAGCAGGTTGAGACAGCTCCCCAGGCCTCCCTGAAGACCGGGTTACAGAAAGG
TGCCAGCGAGATTTCAGATTCCTGTAAGCCCCTAAAGAAAAGGAGTCGCGCCTCAACGGA
TGTAGAGACGGCTAGTTGCACGTACAGAGACACATCTGACTCCGATTCTAGAGGACTGAG
TGACGGGCAGGTAGGCTTTGGCAAACAAGTAGATAGCCCGTCAGCTACTGCTGACGCAGA
TGCTTCTGACGCGCAGTCGGTGGACTCGAGTCTGTCACGGAGAGGTGTCGGCACGAGTAA
GAAGGACACTGTGTGTCAGGTCTGTGAGAAGGCTGGCGACTGCCTTGTTGCTTGTGAGGG
GGAGTGCTGCCGACACTTTCACGTGGAGTGCTTGGGACTGACTGCAGTCCCTGAGGGCCA
CTTCACCTGTGAGGAGTGTGAGACTGGACAGCACCCATGTTTTTCATGTAAAGTGTCTGG
TAAGGATGTGAAACGTTGTTCTGTTAGTGTCTGTGGAAAGTTTTATCATGAAGCCTGTGT
CCGCAAATTCCCCACTGCCATCTTTGAGTCAAAAGGATTCCGCTGTCCCCAGCACTGTTG
CTCTTCCTGCTCTATGGAGAAAGACATCCACAAAGCAAGTAAAGGCCGCATGATGAGGTG
TCTGAGATGCCCGGTCGCCTATCACGTTGGAGATGCGTGTGTCGCTGCTGGAAGTGTATC
TGTGTCCTCGCACATTCTCATCTGTAGTAACCATTCCAAGCGGAGTAGTCAGTCTGCTGC
TATAAACGTAGGCTTTTGTTTCGTTTGTGCCAGAGGGCTGATAGTTCAGGACCATTCAGA
CCCCATGTTCAGTTCCTATGCCTATAAATCCCACTACCTACTGAGTGAATCAAACCGTGC
TGAGTTGATGAAATTACCTATGATTCCTTCTTCGTCAGCTTCCAAAAAGAGATGTGAGAA
AGGTGGAAGATTGCTCTGCTGTGAATCGTGCCCTGCTTCCTTCCACCCAGAATGCCTGAG
CATAGACATGCCAGAAGGCTGCTGGAATTGCAATGACTGTAAAGCTGGCAAGAAACTGCA
TTACAAACAGATTGTTTGGGTCAAATTGGGAAATTACAGATGGTGGCCTGCAGAGATTTG
CAGTCCCAGGTCTGTGCCGCTGAACATCCAGGGCCTTAAGCATGACTTGGGGGACTTCCC
CGTGTTCTTCTTTGGTTCTCACGACTACTACTGGGTTCACCAAGGCAGAGTGTTCCCTTA
CGTTGAAGGAGACAAACACTTTGCAGAGGGACAGACTAGCATTAACAAGACCTTCAAAAA
AGCACTGGAAGAAGCTGCAAAACGTTTCCAGGAGCTGAAAGCCCAGCGTGAAAGCAAGGA
GGCTTTGGAGATGGAGAGGACCTCCAGGAAACCCCCTCCCTACAAGCACATCAAGGCCAA
CAAAGTCATAGGGAAGGTGCAGGTCCAGGTGGCTGACCTGTCGGAGATCCCCCGCTGTAA
CTGCAAGCCTGGTGATGAGAACCCGTGTGGCCTGGAGTCGCAGTGTCTGAACAGGATGTC
ACAGTATGAGTGTCACCCGCAGGTGTGTCCTGCTGGGGACCGCTGTCAGAACCAGTGCTT
TACAAAGAGGCTGTACCCCGATGCAGAGGTCATCAAGACCGAGCGGCGAGGATGGGGCCT
CAGAACCAAAAGAAGCATCAAAAAGGGGGAATTTGTAAACGAGTATGTCGGTGAATTAAT
TGATGAAGAAGAGTGCAGACTCCGAATCAAGCGAGCCCACGAGAACAGTGTAACTAATTT
TTATATGTTAACTGTTACCAAGGACCGTATAATTGATGCTGGACCAAAGGGAAATTATTC
TCGTTTCATGAACCACAGTTGTAATCCAAACTGTGAGACACAGAAGTGGACTGTAAATGG
AGACGTTCGAGTTGGACTATTTGCTCTTTGTGACATTCCTGCAGGGATGGAGTTAACGTT
TAACTATAACTTGGACTGTCTGGGCAACGGCAGGACAGTGTGCCACTGCGGAGCAGACAA
CTGTAGTGGATTTCTAGGAGTGCGGCCAAAGTCTGCATGCACATCAGCAGTTGACGAGAA
GACAAAAAATGCCAAGTTGAAGAAGAGGCGGAAAGTCAAAGCGGAGGCAAAGCCAATCCA
TGAGGATTATTGTTTTCAGTGTGGGGATGGTGGAGAACTGGTCATGTGTGACAAGAAAGA
CTGTCCCAAAGCTTACCACCTCCTATGCCTTAACCTGACTCAGCCACCCCACGGAAAGTG
GGAGTGCCCGTGGCATCGGTGTGACGAGTGTGGCAGTGTAGCCGTTTCCTTCTGTGAATT
CTGTCCACACTCATTCTGTAAGGCTCATGGAAAAGGGGCTCTGGTGCCCTCTGCACTAGA
AGGTCGTCTCTGCTGCTCCAGCCACGACCCTGCATCTCCTGTGTCACCAGAATACTGGAG
CAAGATCAGATGTAAATGGGAATCCCAAGATAGTGGAGAAGAAGTAAAGGAGTAGGGCCT
CATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNHSDIVEDGGPTPFEATLQQGFQYP PTTEDLPPLTNGYPPSISLYETQTKYPPYNQYPNGSANGFGAVRNFSPTDYYHSEIPNTR PHEILEKPSPPQPPPLPPPPPPPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFES SLCGDLLNEVQASEHTKSKHESRKEKRKKSNRHESSRSEERRSHKIPKLEPEGQNRPNER VDTAPEKPREEPVLKEAIPVQPILSSVPTTETSTGVKFQVGDLVWSKVGTYPWWPCMVSS DPQLEVHSKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHEQYEELLAEAAKQASNH SEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERVEQYTFIYIDKQPEEASSQAKKNV TSKTEVKKPRRPRSVLNSQPEQTNAGEVASSQSSTDLRRQSQRRHTSLEEEEPPPVKIAW KTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIG NKTEISVRGQDRLIISSPSQRSEKPAQSASSPEATSGSAGPVEKKQQRRSIRTRSESEKS AEVVPKKKIKKEQVETAPQASLKTGLQKGASEISDSCKPLKKRSRASTDVETASCTYRDT SDSDSRGLSDGQVGFGKQVDSPSATADADASDAQSVDSSLSRRGVGTSKKDTVCQVCEKA GDCLVACEGECCRHFHVECLGLTAVPEGHFTCEECETGQHPCFSCKVSGKDVKRCSVSVC GKFYHEACVRKFPTAIFESKGFRCPQHCCSSCSMEKDIHKASKGRMMRCLRCPVAYHVGD ACVAAGSVSVSSHILICSNHSKRSSQSAAINVGFCFVCARGLIVQDHSDPMFSSYAYKSH YLLSESNRAELMKLPMIPSSSASKKRCEKGGRLLCCESCPASFHPECLSIDMPEGCWNCN DCKAGKKLHYKQIVWVKLGNYRWWPAEICSPRSVPLNIQGLKHDLGDFPVFFFGSHDYYW VHQGRVFPYVEGDKHFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEMERTSRKP PPYKHIKANKVIGKVQVQVADLSEIPRCNCKPGDENPCGLESQCLNRMSQYECHPQVCPA GDRCQNQCFTKRLYPDAEVIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKR AHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCD IPAGMELTFNYNLDCLGNGRTVCHCGADNCSGFLGVRPKSACTSAVDEKTKNAKLKKRRK VKAEAKPIHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPHGKWECPWHRCDECG SVAVSFCEFCPHSFCKAHGKGALVPSALEGRLCCSSHDPASPVSPEYWSKIRCKWESQDS GEEVKE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
338
End on reference sequence
4678
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
