Gene:
Gene Symbol:
Ercc6
Gene Name:
excision repair cross-complementing rodent repair deficiency, complementation group 6
Original Clone ID:
FLH481678.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295431
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4446nts
Open reading frame: 35 to 4480
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTTCCACGAGGAAGTTCCCAACTC
CACTCACCCTCAGGAGCAAGACTGTTTACCAAGTCAGCATGCCAATGCTTACAAAGACAT
GCCAGTTGGGCAGGAGAATGGTGGGGTCTCAGAGGCCGGGGAGTGCCTCTCCTCTACTTC
CTGTGAGTATGGGCCATCCACATCTGCTGAAGCTTGTGTGTTGGCAGCCACAAGGCGTGG
GCCGACCCTGCTACACATTGATCGGCATCAGATCCCTGCAGTTGAGCCCAGTGCTCAGGC
CCTGGAGCTGCAGGGCCTGGGTGTGGACGTCTATGACCAGGCTGTCCTGGAACAAGGCGT
GCTTCAGCAGGTGGACAGTGCGATGCATGAGGCCAGCTGTGTGGCCCAGCTTGCTGATGC
GGAGAAGGAGTATCAGTCAGTCCTGGATGACCTCATGTCATGTACAACATCCCTGAGGCA
AATCAATAAAATTATAGAGCAACTTAGCCCTCAAGCTGCCAGCAACAGAGACATCAACAG
GAAACTAGATTCCGTGAAGCGACAGAAGTATAACAAGGAACAACAGCTAAAGAAGATAAC
TGCAAAACAGAAACGTCTCCAGGCCATTCTCGGAGGGGCTGGGGTCCAAGTGGAACTGGA
TCATGCCAGCCTAGAGGAGGACGATGCAGAGCCAGGGCCATCATGTCTTGGCAGTATGCT
CATGCCTGCCCAGGAGACCGCCTGGGAGGAGCTTATCCGCACTGGCCAGATGACACCGTT
TGGTACCCCAGCCCCTCAGAAACAGGAGAAAAAGCCCAGAAAAATAATGCTGAATGAAGC
ATCAGGCTTTGAAAAATATTTGGCAGAGCAAGCACAACTTTCGTTTGAAAGGAAGAAACA
AGCTGCTACTAAAAGAACAGCTAAAAAAGCTATAGTCATATCCGAGTCAAGCCGAGCAGC
AATTGAAACCAAGGCAGACCAGAGAAGCCAAGTGCTCTCTCAGACAGACAAGCGCCTGAA
GAAGCACAGCAGGAAACTCCAGAGGAGGGCGCTGCAGTTCCAGGGCAAAGTGGGATTGCC
GAGCGGGAAGAAACCTTTGGAGCCTGAGGTGAGGCCAGAGGCCGAGGGAGACACTGAGGG
TGAGGAGTCTGGGTCCTCTCCCACCGATGGGGAGGAGGAGGAAGAACAAGAGGAGGAGGA
AGGGGTAGCCAGCCTGTCTTCTGATGACGTTAGCTATGAGCTGAAGCCTCTTCGGAAGAG
GCAGAAATACCAGAAGAAAGTCCCAGTTCAGGAGATCGACGATGACTTCTTTCCAAGTTC
TGAGGAGGAAGATGAAGCTATGGAAGGAAGAGGAGGAGGCCGAAAGGTAGCCAGGCGCCA
AGACGATGGAGATGAGGATTATTATAAGCAGCGGTTAAGGAGGTGGAACAGGCTGAGGCT
GCAGGACAAAGAGAAACGCCTGAAGCTTGAAGATGATTCTGAGGAGAGCGATGCCGAATT
TGACGAAGGTTTCAAAGTGCCGGGTTTTCTGTTCAAAAAGCTCTTTAAGTATCAGCAGAC
AGGTGTTAGGTGGCTGTGGGAATTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGACGA
AATGGGATTGGGCAAGACCATCCAGATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAA
GATCAGGACTCGCGGTTCAAATTACAGGTTCGAGGGACTAGGCCCCACTATAATTGTCTG
CCCAACGACAGTGATGCATCAGTGGGTGAAGGAATTCCACACGTGGTGGCCTCCATTCAG
AGTGGCAGTTCTGCATGAAACAGGTTCCTACACCCACAAAAAGGAGAGGCTGATTCGAGA
TATTGTTTATTGTCATGGAGTTTTGATAACTTCATATTCATACATCCGACTGATGCAAGA
TGACATTAGCAGACATGACTGGCACTACGTCATCTTAGATGAAGGACACAAAATTCGAAA
TCCAAATGCTGCAGTCACCCTTGCTTGCAAACAGTTCCGGACACCTCATCGGATTATCTT
GTCTGGCTCACCGATGCAGAATAACCTCCGAGAGCTGTGGTCACTCTTCGACTTCATCTT
CCCAGGAAAGCTGGGCACATTGCCTGTGTTTATGGAGCAGTTCTCTGTCCCCATCACCAT
GGGAGGCTATTCCAATGCTTCCCCAGTACAGGTTAAAACTGCTTATAAGTGTGCATGTGT
CTTAAGAGACACGATAAACCCGTACTTACTGCGGAGAATGAAATCAGATGTCAAGATGAG
CCTTTCGTTGCCAGATAAAAATGAGCAGGTCTTATTTTGCCGTCTAACTGATGAGCAGCA
TAAAGTCTACCAGAATTTCATTGATTCTAAAGCAGTCTACAGGATTCTCAATGGAGAAAA
TCAGATTTTCTCTGGACTTGTAGCGCTGAGGAAGATCTGCAACCACCCTGACCTCTTTTC
TGGCGGCCCCAAGAACGCCAGTGGTCCTCCAGAGGATGAGCTGGAAGAAGAGCAGTTTGG
ACACTGGAGACGCTCTGGGAAAATGATTGTTGTTGAGTCTTTGCTGAAAATATGGCACAG
GCAGGGTCAGCGGGTGCTGCTGTTTTCCCAGTCCAGGCAGATGCTACACATACTGGAAGT
GTTCCTGAGAGCCCATAAGTATTCCTATCTCAAGATGGATGGGACCACCACCATAGCGTC
AAGACAGCCGCTGATTACAAAATACAATGAGGACACGTCCATCTTTGTCTTTCTTCTGAC
CACACGGGTGGGTGGCTTAGGAGTGAACCTGACCGGTGCCAACAGAGTCATCATCTACGA
CCCAGACTGGAATCCAAGCACCGACACACAGGCCCGGGAGCGAGCATGGAGGATAGGTCA
GAAGAAGCAGGTGACTGTCTACAGGCTTCTGACGGCTGGCACCATTGAGGAAAAGATCTA
CCACCGACAAATCTTCAAGCAATTTTTGACAAACAGAGTGCTAAAAGACCCAAAACAAAG
GCGCTTTTTCAAATCCAACGATCTCTATGAGCTGTTCACTCTGACCAGTCCTGACGCCTC
CCAGGGCACTGAGACAAGTGCTATTTTTGCAGGAACGGGCTCCAGTATTCAGACACCCAA
ATGCCAGTTAAAAAAAAGGACTTCCACAGTCCTGGGAACAGATCCCAAATGCAAGAAGCC
CCCTGTTTCTGACACACCTGCAAATGCTGCCACCCTGATTGGAGAAAAACCTAAGGCGGC
GGGAGCTACAGGAAGAAGTGTCACTTCTGGAGAAAGTGGTCCTTTCAAAGGCGACCATGA
CACAAATGGGAACAGAGCTAGCAGTGTTGCTTTTGGAGAAGAGACAGATGCAGGGTCCAC
ACTGGAGCATCTCTCAGTGATGAGTGGAGATGGGAAGCATTCAGATTCTCCAACAGTTGA
CCACACATCCAGGCCACCAGTGGAGGCAAGCACCAGTGAGAAGCAGGGTTCTTCCTATGC
AGGGGCGCGCTGCCAGGCTCAGACAGAACCTGTTCCCATGAGTGAACAAATGGAAGGTCA
GTTTTCTAAGTACAAATCCAAAAGGAAGCATGATGCATCTGAGGAAGAGACCACAGAGAA
ACGTCCACAGCCCAAGCAAAAGGCCAAGAACTCCAAGCATTGCAGAGATGCTAAGTTTGA
GGGAACTCGAGTCCCGCACCTGGTGAAGAAAAGACGGTACCGCCAGCAAACCAGTGAGCA
GGAGGGCGGAGCCAAGGACCGGAGCAGCGATGATTATGTGTTGGAAAAGCTTTTCAAAAA
ATCAGTGGGCGTGCACAGCGTCGTGAGGCATGATGCCATCATAGATGGGTCCAGTCCAGA
TTATGTGCTGGTGGAAGCAGAAGCCAACCGAGTGGCTCAGGACGCCTTGAAAGCTCTGAG
GCTCTCTCGTCAGCAATGCCTGGGGGCAGCATCTGGTGTTCCCACCTGGACTGGCCACAG
GGGGATTTCTGGTGCACCAACAGGAGTAAAGAATAGGTTCGGTCAGAAAAGGGACTCCAG
CCTCCCTGTGCAGCATCCTTCATCACTAACAGAAAAGACTCAGAACAACATGAAAAAGGA
GGGAAAAGCTCACACCCCTGAGCACTTCAGTGGCAAAGAAGATGGAGCATCTGTGTCTGG
AGCGCCCAGTTCCTCCTCCCTCTTGGCCAGAATGCGAGCAAGAAACCACATGATTTTGCC
GGAGCGCTTAGAGAGTGACAGTGAACATCTTGCTGAGGCTGCTGCCGTGCCTCCCTGCGG
CACCGAACACGACGACCTTCTGGTGGACATGAGGAACTTCATTGCTTTCCAGGCCCAGGT
GGATGGCCAGGCCAGCACTCAGGAAATCCTGCAGGAGTTTGAGTCCAAGCTGTCCGTGGC
ACAGTCCTGTGTCTTCCGAGAACTGCTGAGAAATCTGTGCAATTTCCATAGAACTCCTGG
TGGGGAAGGGATTTGGAAGCTGAAGCCAGAATACTGCTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MFHEEVPNSTHPQEQDCLPSQHANAYKDMPVGQENGGVSEAGECLSSTSCEYGPSTSAEA CVLAATRRGPTLLHIDRHQIPAVEPSAQALELQGLGVDVYDQAVLEQGVLQQVDSAMHEA SCVAQLADAEKEYQSVLDDLMSCTTSLRQINKIIEQLSPQAASNRDINRKLDSVKRQKYN KEQQLKKITAKQKRLQAILGGAGVQVELDHASLEEDDAEPGPSCLGSMLMPAQETAWEEL IRTGQMTPFGTPAPQKQEKKPRKIMLNEASGFEKYLAEQAQLSFERKKQAATKRTAKKAI VISESSRAAIETKADQRSQVLSQTDKRLKKHSRKLQRRALQFQGKVGLPSGKKPLEPEVR PEAEGDTEGEESGSSPTDGEEEEEQEEEEGVASLSSDDVSYELKPLRKRQKYQKKVPVQE IDDDFFPSSEEEDEAMEGRGGGRKVARRQDDGDEDYYKQRLRRWNRLRLQDKEKRLKLED DSEESDAEFDEGFKVPGFLFKKLFKYQQTGVRWLWELHCQQAGGILGDEMGLGKTIQIIA FLAGLSYSKIRTRGSNYRFEGLGPTIIVCPTTVMHQWVKEFHTWWPPFRVAVLHETGSYT HKKERLIRDIVYCHGVLITSYSYIRLMQDDISRHDWHYVILDEGHKIRNPNAAVTLACKQ FRTPHRIILSGSPMQNNLRELWSLFDFIFPGKLGTLPVFMEQFSVPITMGGYSNASPVQV KTAYKCACVLRDTINPYLLRRMKSDVKMSLSLPDKNEQVLFCRLTDEQHKVYQNFIDSKA VYRILNGENQIFSGLVALRKICNHPDLFSGGPKNASGPPEDELEEEQFGHWRRSGKMIVV ESLLKIWHRQGQRVLLFSQSRQMLHILEVFLRAHKYSYLKMDGTTTIASRQPLITKYNED TSIFVFLLTTRVGGLGVNLTGANRVIIYDPDWNPSTDTQARERAWRIGQKKQVTVYRLLT AGTIEEKIYHRQIFKQFLTNRVLKDPKQRRFFKSNDLYELFTLTSPDASQGTETSAIFAG TGSSIQTPKCQLKKRTSTVLGTDPKCKKPPVSDTPANAATLIGEKPKAAGATGRSVTSGE SGPFKGDHDTNGNRASSVAFGEETDAGSTLEHLSVMSGDGKHSDSPTVDHTSRPPVEAST SEKQGSSYAGARCQAQTEPVPMSEQMEGQFSKYKSKRKHDASEEETTEKRPQPKQKAKNS KHCRDAKFEGTRVPHLVKKRRYRQQTSEQEGGAKDRSSDDYVLEKLFKKSVGVHSVVRHD AIIDGSSPDYVLVEAEANRVAQDALKALRLSRQQCLGAASGVPTWTGHRGISGAPTGVKN RFGQKRDSSLPVQHPSSLTEKTQNNMKKEGKAHTPEHFSGKEDGASVSGAPSSSSLLARM RARNHMILPERLESDSEHLAEAAAVPPCGTEHDDLLVDMRNFIAFQAQVDGQASTQEILQ EFESKLSVAQSCVFRELLRNLCNFHRTPGGEGIWKLKPEYC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4535
Start on reference sequence
90
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
