Gene:
Gene Symbol:
Itga1
Gene Name:
integrin alpha 1
Original Clone ID:
FLH481681.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295434
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3540nts
Open reading frame: 35 to 3574
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGTCCCCAGGCGTCCTGCTAGCCT
AGAGGTCACTGTAGCCTGCATCTGGCTTCTCACCGTTATCCTAGGCGTCTGCATCTCCTT
CAACGTTGATGTCAAAAACTCCATGAGCTTCAGTGGTCCAGTGGAGGACATGTTTGGATA
CACCGTTCAACAGTATGAAAACGAAGAAGGCAAATGGGTTCTTATTGGTTCTCCTTTAGT
TGGCCAACCCAAAGCAAGAACTGGAGATGTCTATAAGTGTCCAGTTGGGAGAGAGAGATC
AATGCCTTGTGTGAAGTTGGATTTACCAGTTAACACATCGATTCCTAATGTCACAGAAAT
AAAGGAAAACATGACATTTGGATCAACTTTAGTCACCAATCCGAAGGGAGGATTTCTGGC
ATGTGGGCCCTTGTATGCCTATAGATGTGGACATTTGCATTATACGACTGGCATATGTTC
TGATGTCAGCCCTACATTTCAAGTTGTGAACTCGTTCGCCCCTGTACAAGAATGTAGCAC
ACAGCTTGACATAGTCATCGTCCTAGATGGCTCCAACAGCATCTACCCCTGGGAAAGTGT
CACTGCCTTTTTAAACGACCTTCTTAAGAGGATGGACATTGGCCCCAAACAGACACAGGT
TGGAATTGTACAATATGGAGCAAATGTAACACATGAGTTCAACCTCAATAAGTATTCATC
CACAGAAGAGGTCCTGGTGGCAGCAAACAAAATAGGCCGCAGGGGTGGTCTCCAGACGAT
GACAGCCCTTGGAATAGACACAGCCAGGAAAGAGGCATTCACTGAAGCTCGGGGGGCCAG
AAGGGGAGTGAAAAAAGTCATGGTTATTGTGACTGATGGAGAATCGCATGACAACTATCG
CCTGAAACAGGTCATCCAAGACTGTGAGGATGAAAACATTCAGCGATTTTCCATAGCTAT
CCTTGGCCACTATAACAGGGGGAACTTAAGCACCGAAAAATTTGTGGAGGAGATAAAATC
AATCGCAAGTGAGCCCACCGAAAAGCACTTCTTCAACGTCTCAGATGAGTTGGCCCTGGT
CACTATTGTTAAAGCTCTGGGAGAAAGGATATTTGCTTTGGAAGCTACAGCTGACCAGTC
AGCAGCTTCGTTTGAAATGGAGATGTCTCAGACCGGCTTCAGTGCTCATTATTCACAGGA
CTGGGTCATGCTTGGAGCTGTTGGAGCCTATGACTGGAACGGAACTGTGGTCATGCAGAA
GGCTAACCAGATTGTCATCCCTCATAACACCACCTTTCAAACTGAGCCCACCAAGATGAA
CGAGCCTCTTGCTTCTTACTTGGGTTACACAGTGAACTCTGCCACCATCCCGGGAGATGT
GCTCTATATCGCTGGGCAGCCTCGGTACAATCACACCGGCCAGGTCGTCATCTACAAGAT
GGAGGATGGGGACGTCAACATTCTGCAGACACTCAGTGGAGAGCAGATCGGATCCTACTT
TGGTAGCGTCTTAACCACAATTGACATCGACAAAGATTCTTACACTGATCTGCTTCTCGT
CGGGGCTCCCATGTATATGGGGACAGAGAAAGAGGAACAGGGCAAGGTGTACGTGTACGC
TGTGAATCAGACGAGGTTTGAATATCAAATGAGCCTGGAACCAATTAAGCAGACCTGCTG
TTCATCCCTGAAGGATAATTCATGCACAAAAGAAAACAAGAATGAGCCCTGTGGGGCCCG
TTTTGGAACAGCAGTTGCTGCTGTAAAAGACCTCAACGTGGATGGCTTTAATGACGTCGT
GATTGGAGCTCCCCTGGAAGACGACCACGCAGGAGCTGTGTACATTTATCATGGCAGTGG
CAAGACCATAAGGAAAGAGTATGCGCAACGCATTCCCTCAGGTGGGGATGGGAAGACGCT
GAAATTTTTTGGCCAGTCTATCCACGGAGAGATGGATTTAAATGGCGATGGTCTGACTGA
CGTGACCATTGGAGGCCTTGGAGGAGCTGCCCTCTTCTGGGCCAGAGATGTGGCTGTAGT
TAAAGTGACCATGAATTTTGAACCCAACAAAGTGAATATTCAAAAGAAAAACTGCCGTGT
GGAGGGCAAAGAAACAGTATGCATAAATGCTACAATGTGTTTTCATGTGAAATTGAAGTC
TAAAGAAGACTCAGTTTACGAGGCTGATCTGCAGTACCGTGTCACCCTTGATTCGCTGAG
GCAGATATCACGGAGCTTTTTTTCTGGAACTCAGGAAAGGAGGATTCAAAGAAACCTCAC
CGTTCGAGAATCCGAATGCATCAGGCACTCCTTCTACATGTTGGATAAGCACGACTTTCA
GGACTCCGTGAGAGTGACTTTGGATTTTAACCTCACTGATCCAGAAAATGGGCCCGTGCT
CGATGACGCTCTGCCAAACTCAGTCCATGGACATATTCCTTTTGCCAAAGACTGTGGGAA
CAAGGAAAGATGCGTTTCAGACCTCACCCTGGATGTGTCCACAACAGAAAAGAACCTGCT
GATTGTCAGATCCCAGAATGACAAGTTCAATGTCAGCCTCACCGTCAAAAACAAGGGAGA
CAGTGCGTACAACACCCGGACAGTGGTTCAGTATTCTCCAAATCTGATTTTTTCAGGAAT
TGAGGAGATCCAAAAAGATAGCTGCGAATCCAATCAAAATATCACGTGTAGAGTTGGATA
TCCTTTCCTGAGGACAGGAGACGTGGTTAACTTCAAAATAATATTCCAGTTTAATACATC
ACATCTCTCAGAAAATGCAATTATTCATTTAAGTGCAACAAGTGACAGTGAAGAGCCCCT
GGAATCTCTTTACGATAATGAAGTAAATATTTCCATCCCCGTAAAATATGAAGTCGGACT
GCAGTTTTACAGTTCTGCGAGTGAACACCACATTTCAGTTGCTGCCAATGAGACTGTTCC
TGAGCTGATTAATTCCACCAAGGACATTGGGGATGAAATTAATGTCTTCTACACGATTAG
AAAAAGAGGGCATTTCCCAATGCCAGAACTTCGGCTGGCAATTTCATTTCCCAATCTGAC
GTCAGATGGCTATCCTGTACTGTACCCAACTGGATGGTCATCTTCTGATAATGTAAACTG
CAGACCTCGAAGCCTTGAGGACCCTTTGGGTATCAACTCTGGGAAGAAAATGACAATATC
AAAGTCTGAGGTTCTCAAAAGAGGCACAATCCAGGACTGCAGTACCTGCAAGATTGCTAC
CATCACGTGTCATCTCCTTCCCTCGGATGTGAGTCAAGTGAATGTCTCACTCATCTTGTG
GAAACCAACTTTCATAAAAGCACATTTTTCCAGCTTAAATCTTACCATACGGGGAGAACT
TCAGAGTGAAAACTCATCGCTGACTTTAAGCAGCAGCAACCGGAAACGAGAGCTGGCTAT
TCAGATATCCAAAGACGGGCTCCCAGGCCGAGTGCCGCTGTGGGTTATCCTCCTGAGCGC
CTTTGCGGGACTCCTGCTGTTAATGCTGCTTATATTAGCTCTGTGGAAGATTGGATTCTT
CAAAAGGCCACTAAAGAAGAAAATGGAGAAATAGGGCCTCATGGGCCCAGCTTTCTTGTA
C
Protein sequence predicted based on nucleotide sequence
MVPRRPASLEVTVACIWLLTVILGVCISFNVDVKNSMSFSGPVEDMFGYTVQQYENEEGK WVLIGSPLVGQPKARTGDVYKCPVGRERSMPCVKLDLPVNTSIPNVTEIKENMTFGSTLV TNPKGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSFAPVQECSTQLDIVIVLDGS NSIYPWESVTAFLNDLLKRMDIGPKQTQVGIVQYGANVTHEFNLNKYSSTEEVLVAANKI GRRGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNYRLKQVIQDCEDE NIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKALGERIF ALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKANQIVIPHNTT FQTEPTKMNEPLASYLGYTVNSATIPGDVLYIAGQPRYNHTGQVVIYKMEDGDVNILQTL SGEQIGSYFGSVLTTIDIDKDSYTDLLLVGAPMYMGTEKEEQGKVYVYAVNQTRFEYQMS LEPIKQTCCSSLKDNSCTKENKNEPCGARFGTAVAAVKDLNVDGFNDVVIGAPLEDDHAG AVYIYHGSGKTIRKEYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAAL FWARDVAVVKVTMNFEPNKVNIQKKNCRVEGKETVCINATMCFHVKLKSKEDSVYEADLQ YRVTLDSLRQISRSFFSGTQERRIQRNLTVRESECIRHSFYMLDKHDFQDSVRVTLDFNL TDPENGPVLDDALPNSVHGHIPFAKDCGNKERCVSDLTLDVSTTEKNLLIVRSQNDKFNV SLTVKNKGDSAYNTRTVVQYSPNLIFSGIEEIQKDSCESNQNITCRVGYPFLRTGDVVNF KIIFQFNTSHLSENAIIHLSATSDSEEPLESLYDNEVNISIPVKYEVGLQFYSSASEHHI SVAANETVPELINSTKDIGDEINVFYTIRKRGHFPMPELRLAISFPNLTSDGYPVLYPTG WSSSDNVNCRPRSLEDPLGINSGKKMTISKSEVLKRGTIQDCSTCKIATITCHLLPSDVS QVNVSLILWKPTFIKAHFSSLNLTIRGELQSENSSLTLSSSNRKRELAIQISKDGLPGRV PLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3973
Start on reference sequence
434
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
