Gene:
Gene Symbol:
Col28a1
Gene Name:
collagen, type XXVIII, alpha 1
Original Clone ID:
FLH481689.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295442
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3426nts
Open reading frame: 35 to 3460
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGGAGAAGAGACGTTGCCTTCTG
TCTTCTGCTTTTGCCAGCTTTTATGACTCAAGCAGTGTATGGACAAAGAAAGAAAGGACC
AAAGCCAAACACACTTGCAAGGAAAAATGACTTCCAGGATGCCATTTGCTTCATAGATGT
TGTCTTTATCTTGGACAGCTCAGAAAGCTCCAAAATTGTACTCTTTGATAATCAGAAAGA
TTTTGTGGACAGCTTGAGTGAGAAGATTTTCCAGTTGACTCCTGGCCGTTCATTAAAATA
TGACATCAAGCTGGCAGCCCTTCAGTTTAGCAGCTCGGTCCAGATTGATCCACCCTTGTC
TTCTTGGAAGGATCTCCGGACATTTAAGCAGCGGGTCAAGTCTCTGAATTTAATTGGGCA
AGGTACCTTTTCTTATTATGCCATCTCCAATGCCACAAGGCTACTTAAAAGAGAAGGGCG
TAAAGATGGCGTGAAGGTGGCTTTGCTGATGACTGATGGCATCGATCATCCCAAGAGCCC
AGATGTCCAAAGTATTTCTGAAGATGCTAGAATTTTGGGAATATCATTTATCACCGTTGG
ACTTTCCACTGTGGTCAATGAAGCCAAACTTCGTTTGATATCTGGGGATCCTTCCAATGA
ACCCGTTCTACTATTGAGTGACCCAACACTTGTAGACAGAATTCAGGAACGCCTGGGTGT
CTTGTTTGAAAGGAAGTGTGAACACAAGATCTGTGAATGTGAAAAGGGCGAGCCAGGTGA
CCCAGGGCCTCCTGGTACCCATGGAAACCCTGGTATCAAAGGTGAGCGAGGACCAAAAGG
AAACCCGGGTGATGCTCAGAAAGGAGAAACTGGAGAAAGAGGTCCTGTGGGAATCCCTGG
GTACAAGGGTGACAAGGGTGAACGAGGAGAATGTGGGAAACCAGGAATGAAAGGTGACAA
AGGACCTGAAGGGCCATATGGACCAAAGGGACCAAGAGGAATTCAGGGCATTGGTGGACC
TCCAGGGGATCCAGGTCCAAAGGGATTTCAAGGAAATAAGGGTGAGCCAGGTCCTCCTGG
TCCTTATGGTCCTCCAGGAGCCCCTGGTATTGGACAGCAAGGTGTTAAGGGTGAGAGGGG
CCAGGAAGGAAGAATGGGAGCTCCCGGACCGATTGGAATTGGTGAACCCGGACAGCCAGG
TCCCCGTGGTCCTGAGGGAGCACCAGGAGAGCGGGGCCTACCAGGAGAAGGATTTCCTGG
ACCTAAGGGTGAAAAGGGATCTGAAGGACCAATCGGCCCACAAGGATTACAAGGCCTGTC
AATCAAAGGGGATAAGGGGGATTTGGGACCTGTGGGACCGCAGGGGCCAGCGGGAATTCC
TGGCATCGGAAGCCAAGGCGAGCAGGGGATCCAAGGTCCATCTGGTCCACCTGGTCCTCA
AGGACCCCCAGGACAAGGCTCACCTGGTCCTAAGGGAGAAGTAGGTCAGATGGGACCCAC
AGGGCCTAGAGGACCAATGGGCATTGGAGTACAGGGTCCAAAGGGGGAGCCAGGCACAGT
GGGGCTCCCAGGACAGCCTGGAGTCCCAGGGGAAGATGGGGCCTCTGGAAAGAAGGGAGA
AGCTGGACTTCCAGGAACAAGAGGCCCAGAAGGAATGCCTGGGAAAGGACAGCCCGGCCC
CAAGGGTGATGAAGGAAAGAAAGGGAGCAAAGGAAATCAAGGACAGAGGGGGTTTCCAGG
ACCCGAAGGGCCAAAGGGAGAGCCAGGTGTTATGGGCCCCTTCGGAATGCCCGGAGCCTC
AATTCCTGGACCATCTGGACCAAAGGGAGACCGAGGAGGACCTGGCATGCCTGGTCTTAA
AGGAGAACCTGGACTTCCTGTTCGAGGACCAAAGGGTGCCCAGGGTCCCCGAGGACCAGT
GGGGGCTCCAGGACTCAAAGGTGATGGCTATCCTGGTGTGGCTGGACCGCGAGGATTGCC
AGGACCCCCTGGACCAATGGGCTTACGTGGTGTGGGAGACACTGGAGCAAAGGGAGAACC
AGGAGTCAGAGGCCCTCCAGGTCCCTCTGGGCCTCGGGGAATAGGAACCCAAGGACCAAA
GGGTGATACTGGGCAAAAGGGCCTGCCTGGCCCTCCTGGGCCCCCTGGCTATGGATCACA
AGGAATTAAAGGGGAGCAAGGACCTCAAGGTTTCCCAGGGTCAAAGGGCACAGTGGGCCT
TGGCCTCCCTGGTCAGAAGGGAGAGCATGGAGACCGGGGTGATGTGGGAAGGAAAGGCGA
AAAGGGAGAAACTGGAGAGCCTGGGTCTCCAGGAAAACAGGGGTTACAAGGACCTAAAGG
AGACCTGGGACTCACAAAAGAAGAAATTATCAAACTTATCATTGAAATATGTGGTTGTGG
GCCCAAATGCAAAGAGACTCCACTGGAGTTGGTGTTTGTGATTGACAGTTCAGAAAGTGT
GGGACCAGAGAACTTTCAGATCATCCAAAGTTTTGTGAAGACTTTGGCTGACCGGGTGGC
CTTGGACCTTGGCACAGCCCGGATAGGCATAATCAACTATAGCCACAAGGTGGAGAAGGT
GGCTAGCCTGAAGCAGTTTTCCAGCAAGGATGACTTCAAGCTGGTGGTAGACAACATGCA
GTATCTGGGGGAAGGCACCTATACAGCCACTGCTCTTCAAGCAGCTAATGACATGTTCAA
AGAGGCAAGGCCAGGTGTAAAGAAGGTGGCCTTGGTGATCACGGATGGACAGACAGATTC
CCGAGATAAAAAGAAGCTGGCAGATGTGGTGAAGGATGCCAATGACTCCAACGTGGAAAT
ATTTGTCATTGGAGTGGTGAAGAAGGATGACCCCAACTTTGAAATTTTTCACAAAGAAAT
GAATCTCATTGCTACTGATGCAGAACATGTGTATCAGTTTGACGACTTCTTTACCCTGCA
AGATACTCTGAAGCAAAAGTTGTCTAAAAAAATTTGTGAAGATTTTGACTCCTATCTCAT
TCAAGTTTTTGGATCACCATCTTTTCAACCTGAATTTGGGGTATCAGAACGAGAGGTCAG
CGTATCCACTCCAAAGCCTGCAAAAGAAATGTCTAAGTCGTTTAATGTTTCTAGAGGCCA
AAATGAAGAGACTGAATCGTATGTGTTGACTGAGGCTGGTATCTTGGCCATCCCAACCCC
ACCTGAAGCCACCAATACACTTGAGCCATTACTCAGCAGCCGTGAAGGAGTGGAAACAAG
AACTCCAAATCCCAATTTGTTACAGTCAGAAAAATCTCTGTACAAAGATCCAAGATGCGA
GGAAGCCCTGAAGCCCGGGGAATGTGGCGACTACGTGGTTCGCTGGTATTACGACAAACA
GGTCAACTCTTGCGCTCGCTTTTGGTTCAGTGGCTGCAATGGCTCAGGAAACAGATTCCA
CAGTGAAAAAGAATGTCGAGAAACCTGCATTAAACAATAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MRRRDVAFCLLLLPAFMTQAVYGQRKKGPKPNTLARKNDFQDAICFIDVVFILDSSESSK IVLFDNQKDFVDSLSEKIFQLTPGRSLKYDIKLAALQFSSSVQIDPPLSSWKDLRTFKQR VKSLNLIGQGTFSYYAISNATRLLKREGRKDGVKVALLMTDGIDHPKSPDVQSISEDARI LGISFITVGLSTVVNEAKLRLISGDPSNEPVLLLSDPTLVDRIQERLGVLFERKCEHKIC ECEKGEPGDPGPPGTHGNPGIKGERGPKGNPGDAQKGETGERGPVGIPGYKGDKGERGEC GKPGMKGDKGPEGPYGPKGPRGIQGIGGPPGDPGPKGFQGNKGEPGPPGPYGPPGAPGIG QQGVKGERGQEGRMGAPGPIGIGEPGQPGPRGPEGAPGERGLPGEGFPGPKGEKGSEGPI GPQGLQGLSIKGDKGDLGPVGPQGPAGIPGIGSQGEQGIQGPSGPPGPQGPPGQGSPGPK GEVGQMGPTGPRGPMGIGVQGPKGEPGTVGLPGQPGVPGEDGASGKKGEAGLPGTRGPEG MPGKGQPGPKGDEGKKGSKGNQGQRGFPGPEGPKGEPGVMGPFGMPGASIPGPSGPKGDR GGPGMPGLKGEPGLPVRGPKGAQGPRGPVGAPGLKGDGYPGVAGPRGLPGPPGPMGLRGV GDTGAKGEPGVRGPPGPSGPRGIGTQGPKGDTGQKGLPGPPGPPGYGSQGIKGEQGPQGF PGSKGTVGLGLPGQKGEHGDRGDVGRKGEKGETGEPGSPGKQGLQGPKGDLGLTKEEIIK LIIEICGCGPKCKETPLELVFVIDSSESVGPENFQIIQSFVKTLADRVALDLGTARIGII NYSHKVEKVASLKQFSSKDDFKLVVDNMQYLGEGTYTATALQAANDMFKEARPGVKKVAL VITDGQTDSRDKKKLADVVKDANDSNVEIFVIGVVKKDDPNFEIFHKEMNLIATDAEHVY QFDDFFTLQDTLKQKLSKKICEDFDSYLIQVFGSPSFQPEFGVSEREVSVSTPKPAKEMS KSFNVSRGQNEETESYVLTEAGILAIPTPPEATNTLEPLLSSREGVETRTPNPNLLQSEK SLYKDPRCEEALKPGECGDYVVRWYYDKQVNSCARFWFSGCNGSGNRFHSEKECRETCIK Q*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
131
End on reference sequence
3556
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
