Gene:
Gene Symbol:
Srebf2
Gene Name:
sterol regulatory element binding factor 2
Original Clone ID:
FLH481703.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00295456
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3393nts
Open reading frame: 35 to 3427
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATGAGAGCAGCGAGCTGGGCGT
TCTGGAGACCATGGAGACCCTCACGGAGCTGGGCGATGAGCTGACTCTCGGGGACATCGA
CGAGATGCTACAGTTTGTCAGCAATCAAGTGGGAGAGTTCCCTGATTTGTTTTCAGAGCA
ACTTTGTAGCTCCTTCCCTGGAGGTGGCAGCAATGGTGGCAGTGGCAACAACAGCAGTGG
CAGAGGCAACAATGGTGGAGCCACAGACCCTGCAGTACAGCGGTCATTCAGCCAGGTCCC
ATTATCTACCTTCTCTCCCTCAGCTGCCTCCCCTCAGGCTCCGGCCCTGCAGGTCAAAGT
CTCTCCTACCCCACCAAGGGCAACCCCTGTTCTCCAGCCGCGACCCCAGCCCCAGCCCCA
ACCTCCAGCTCAGCTGCAGCAGCAGACGGTGATGATCACCCCGACGTTCAGCACCGCTCC
GCAGACGAGGATCATCCAGCAGCCTTTGATATACCAGAACGCAGCGACCAGCTTTCAAGT
CCTGCAGCCTCAAGTGCAAAGCCTCGTGACGTCCCCGCAGGTGCAGCCCGTCACCATCCA
GCAGCAGGTGCAGACGGTACAGGCCCAGCGGGTGTTGACGCAGACAGCCAATGGCACACT
GCAGACCCTTGCCCCCGCCACGGTACAGACGGTCGCTGCGCCACAGGTGCAGCAGGTCCC
GGTCCTGGTGCAACCTCAGATCATTAAGACAGATTCCCTTGTGTTGACCACGCTGAAGAC
AGATGGCAGCCCTGTCATGGCTGCAGTCCAGAACCCGGCCCTCACGGCCCTCACTGCCCC
CATCCAGACAGCCGCCCTTCAAGTGCCAACCCTGGTGGGCAGCAACGGGACCATTCTGAC
CACAATGCCTGTGATGATGGGGCAAGAGAAAGTTCCTATCAAGCAAGTGCCTGGCGGCGT
GAAGCAGCTGGATCCTCCCAAAGAAGGAGAGAGGCGGACAACACACAATATCATTGAAAA
GCGCTACCGGTCCTCCATCAACGACAAAATCATAGAGTTGAAGGACTTAGTCATGGGGAC
AGATGCCAAGATGCACAAGTCTGGCGTTCTGAGGAAGGCCATTGATTACATCAAATATCT
GCAGCAGGTCAATCACAAGCTGCGCCAGGAGAACATGGTGCTGAAGCTGGCCAATCAGAA
AAACAAGCTCCTGAAGGGCATCGACCTGGGCAGTCTGGTGGACAGTGATGTGGACTTGAA
AATTGATGACTTTAACCAGAATGTCCTTCTGATGTCTCCGCCGGCCTCCGACTCCGGGTC
CCAGGCCGGCTTCTCTCCCTATTCCATTGACTCTGAGCCGGGCAGCCCTCTGCTGGATGA
CGCAAAGGTCAAGGATGAACCGGACTCTCCTCCTGTGGCACTGGGCATGGTGGACCGCTC
TCGAATCCTCTTATGTGTCCTCACCTTCCTGGGCCTCTCCTTTAACCCCTTGACTTCCTT
GCTGCAGTGGGGAGGGGCCCACAACACTGACCAGCACCCATACTCAGGCTCGGGCCGCAG
TGTACTGTCACTGGAGTCAGGTGCTGGGGGCTGGTTTGACTGGATGGTTCCAACTCTCCT
CCTGTGGCTGGTAAATGGTGTGATTGTCTTGAGCGTCTTTGTGAAGCTGTTGGTCCACGG
GGAGCCGGTGATTCGCCCACACTCACGCCCCTCAGTCACCTTCTGGAGACACCGGAAGCA
GGCAGACCTAGACCTCGCCAAAGGTGATTTCGCAGCTGCTGCTGCCAACCTACAAACCTG
CTTATCCGTGCTGGGCCGGGCGCTGCCCACCTCCCGCCTGGACTTGGCCTGCAGCCTCTC
CTGGAATGTGATCCGCTACAGCCTGCAGAAGCTGCGCCTGGTACGCTGGTTACTCAAGAA
GGTCTTCCAGCGCTGGCGGGCTACTCCCGCCACTGCAGCTGGCTTTGAGGATGAGGCTAA
GAGCAGCGCAAGGGATGCGGCACTGGCCTACCACAGGCTGCACCAGCTGCACATCACAGG
GAAGCTTCCGGCGGGATCTGCCTGTTCAGATGTGCACATGGCTCTGTGTGCTGTGAACCT
GGCCGAGTGTGCGGAGGAGAAAATCCTACCAAGCACACTGATTGAGATCCACCTGACAGC
GGCCATGGGGCTCAAGACCCGCTGTGGAGGCAAGCTGGGCTTCTTGGCTAGCTACTTCTT
AAATCGTGCCCAGAGTCTGTGTGGCCCAGAGCATAGCACTGTCCCCGACTCCCTGCGCTG
GCTATGCCATCCCTTGGGCCAGAAGTTTTTCATGGAGCGAAGCTGGTCGATCAAGTCAGC
AGCCAAGGAGAGCCTGTACTGCGCCCAGAGGAGCCCAGCTGACCCCATTGCCCAGGTACA
CCAGGCGTTCTGCAAAAACCTGCTAGAAAGAGCGGTGGAGTCCTTGGTGAAACCTCAGGC
CAAGAAGAAGGCAGGCGACCAGGAAGAAGAGAGCTGTGAATTTTCCAGTGCTCTAGAGTA
TTTGAAATTACTTCATTCTTTTGTGGACTCTGTGGGATTTGTGACCTCACCATTCTCCAG
CAGTTCCGTGCTCAGGTCGGCCCTTGGCCCCGATGTCATCTGCCGGTGGTGGACGTCCGC
AGTCACCATGGCCATCAGCTGGCTCCAGGGAGACGATGCAGCTGTGCGCTCTCGTTTTAC
TGAAGTAGAGCGGGTCCCCAAGGCCCTGGAAGTGACCGAGAGTCCCTTGGTGAAGGCCGT
CTTCTATACCTGTAGAGCCATGCATGCCTCCCTCTCTGGGAAAGCTGACGGGCAGCAGAA
CTCCTTCTGTCACTGTGAGAGGGCCAGTGGTCACCTGTGGAGCAGTCTCAACGTCAGCGG
CACTACTTCTGATCCTTCACTTAACCATGTGATCCAGCTGTTCACCTGTGACCTGCTACT
GTCGCTGAGGACAGCACTCTGGCAAAAGCAGGCCAGTGCCAGCCAGCTCCTGGGTGAGAC
CTACCATGCATCCGGCACTGAGCTGGCCGGCTTCCAGCGGGACCTGGGCAGCCTTCGAAG
GCTGGCCCATAGCTTCCGCCCAGCCTACCGCAAGGTGTTCCTGCATGAAGCCACTGTGCG
CCTGATGGCAGGAGCCAGCCCTACCCGTACACACCAGCTGCTGGAGCATAGCCTACGGCG
GCGCCCCACTCAGAACACCAAGCATGGAGAGGTAGACACCTGGCCTGGCCAACGAGAGCG
AGCTACTGCCATCCTGCTGGCCTGCCGCCATCTTCCCCTCTCTTTCCTGTCTTCCCCGGG
CCAGCGGGCAGTGCTGCTGGCTGAAGCTGCCCGCACCCTGGAGAAGGTGGGTGACCGCCG
TTCCTGCAGTGACTGCCAGCAGATGATTGTCAAGCTGGGCGGTGGCACTGCCATCGCTGC
CTCCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDESSELGVLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGGGSN GGSGNNSSGRGNNGGATDPAVQRSFSQVPLSTFSPSAASPQAPALQVKVSPTPPRATPVL QPRPQPQPQPPAQLQQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTS PQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTD SLVLTTLKTDGSPVMAAVQNPALTALTAPIQTAALQVPTLVGSNGTILTTMPVMMGQEKV PIKQVPGGVKQLDPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLR KAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDSDVDLKIDDFNQNVLLM SPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLG LSFNPLTSLLQWGGAHNTDQHPYSGSGRSVLSLESGAGGWFDWMVPTLLLWLVNGVIVLS VFVKLLVHGEPVIRPHSRPSVTFWRHRKQADLDLAKGDFAAAAANLQTCLSVLGRALPTS RLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQRWRATPATAAGFEDEAKSSARDAALAYH RLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKILPSTLIEIHLTAAMGLKTRCGGK LGFLASYFLNRAQSLCGPEHSTVPDSLRWLCHPLGQKFFMERSWSIKSAAKESLYCAQRS PADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSV GFVTSPFSSSSVLRSALGPDVICRWWTSAVTMAISWLQGDDAAVRSRFTEVERVPKALEV TESPLVKAVFYTCRAMHASLSGKADGQQNSFCHCERASGHLWSSLNVSGTTSDPSLNHVI QLFTCDLLLSLRTALWQKQASASQLLGETYHASGTELAGFQRDLGSLRRLAHSFRPAYRK VFLHEATVRLMAGASPTRTHQLLEHSLRRRPTQNTKHGEVDTWPGQRERATAILLACRHL PLSFLSSPGQRAVLLAEAARTLEKVGDRRSCSDCQQMIVKLGGGTAIAAS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
135
End on reference sequence
3527
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
