Gene:
Gene Symbol:
NUP188
Gene Name:
nucleoporin 188kDa
Original Clone ID:
FLH481799.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295457
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGGCGGCCGCCGGCGGGCCGTGTGTGAGGAGCAGTAGAGAACTGTGGACTATTCTG
CTTGGAAGGTCAGCTCTGAGAGAGCTGAGTCAGATTGAGGCAGAACTGAATAAACATTGG
CGGCGATTGTTAGAGGGGCTTTCTTACTACAAACCTCCCAGTCCAAGTTCAGCTGAAAAA
GTGAAAGCTAATAAAGATGTAGCTTCACCATTGAAGGAACTGGGTTTAAGAATCAGCAAG
TTTTTGGGTCTTGATGAAGAACAGAGTGTGCAGTTACTCCAGTGTTACCTGCAAGAGGAC
TACAGGGGTACTCGGGACTCAGTAAAGACAGTACTGCAAGATGAGAGGCAGAGCCAGGCC
TTAATCCTGAAGATTGCAGATTATTATTATGAAGAAAGAACCTGTATTCTTCGTTGTGTC
TTACACCTTCTCACTTACTTCCAAGATGAAAGACACCCCTATAGGGTTGAATATGCAGAC
TGTGTTGATAAATTGGAGAAGGAACTAGTTTCAAAATACAGACAGCAGTTCGAAGAGCTT
TATAAAACTGAAGCACCAACTTGGGAGACACATGGAAATCTCATGACAGAGCGCCAAGTG
TCTCGCTGGTTTGTTCAGTGCCTTCGGGAACAGTCCATGCTGCTAGAAATTATTTTCCTT
TATTATGCATACTTTGAGATGGCACCCAGTGACTTACTTGTATTAACCAAGATGTTTAAA
GAGCAAGGATTTGGTAGTAGGCAGACCAATAGGCACCTGGTGGATGAGACTATGGATCCT
TTTGTAGATCGGATTGGCTACTTCAGTGCCCTCATCCTGGTGGAGGGCATGGATATCGAG
TCCTTGCATAAGTGTGCTTTGGATGACAGAAGAGAACTGCATCAGTTTGCGCAGGATGGG
CTTATTTGTCAGGATATGGACTGTTTAATGTTGACCTTTGGGGACATTCCACATCATGCC
CCAGTGCTTTTGGCCTGGGCTCTCCTCCGTCACACTCTGAACCCAGAAGAGACAAGCAGT
GTGGTCCGGAAGATAGGTGGCACAGCCATCCAGCTGAATGTGTTTCAGTACTTGACCCGA
TTGCTCCAGTCCCTTGCCAGTGGGGGAAATGATTGCACCACCAGCACTGCATGCATGTGT
GTCTATGGACTGCTCTCTTTCGTTCTGACCTCGTTGGAGCTGCACACCCTGGGCAATCAG
CAGGATATAATTGATACAGCATGTGAAGTATTGGCCGACCCTTCTCTTCCGGAACTGTTC
TGGGGAACAGAGCCAACTTCTGGCCTTGGGATCATTCTGGACAGTGTGTGTGGAATGTTT
CCCCACCTTCTCTCCCCACTCCTGCAACTGCTCCGAGCCCTGGTATCAGGGAAGTCCACA
GCCAAAAAGGTGTATAGCTTCTTGGATAAGATGTCTTTCTACAATGAACTTTATAAACAC
AAGCCTCATGATGTGATCTCCCATGAAGATGGAACTCTTTGGCGGAGACAAACACCCAAA
CTCCTTTATCCCCTTGGGGGTCAAACCAACCTTCGCATACCTCAAGGCACTGTGGGCCAA
GTAATGTTGGATGATAGGGCATACCTGGTACGCTGGGAATACTCCTATAGCAGCTGGACC
CTCTTTACCTGCGAGATTGAAATGTTGCTTCATGTTGTTTCAACTGCAGATGTGATTCAG
CACTGCCAGCGAGTCAAACCCATCATTGATCTCGTCCATAAGGTCATCAGTACAGACCTG
TCGATAGCAGACTGTCTCCTGCCCATCACATCTCGCATCTACATGCTGCTGCAGCGGTTA
ACGACAGTGATCTCCCCACCTGTGGATGTCATTGCTTCTTGTGTCAACTGCTTAACTGTT
TTGGCTGCCCGCAATCCAGCAAAGGTCTGGACTGATCTTCGTCACACAGGTTTTTTACCA
TTTGTGGCCCATCCTGTCTCCAGCCTGAGTCAGATGATTAGTGCGGAAGGGATGAATGCT
GGAGGGTACGGAAACCTCTTGATGAACAGTGAACAGCCTCAGGGCGAGTATGGGGTTACT
ATTGCCTTTCTGCGCTTGATCACCACCCTTGTCAAGGGGCAACTTGGTAGTACCCAGAGC
CAAGGACTTGTACCCTGTGTAATGTTTGTGCTGAAGGAGATGCTTCCCAGCTACCATAAG
TGGCGCTACAACTCTCATGGAGTGAGGGAACAGATTGGTTGCCTGATCTTGGAGCTGATT
CATGCGATACTGAACCTGTGCCACGAGACAGACCTGCACAGCAGTCATACTCCCAGCCTG
CAGTTTCTCTGCATCTGCAGCCTGGCATACACAGAAGCAGGACAGACAGTTATCAATATC
ATGGGCATTGGCGTGGACACCATTGACATGGTGATGGCTGCTCAGCCTCGAAGTGATGGG
GCAGAGGGCCAGGGGCAGGGCCAGCTGCTGATCAAGACAGTGAAACTGGCATTCTCCGTC
ACCAACAATGTTATTCGGCTGAAACCTCCTTCTAATGTGGTGTCCCCCCTGGAACAGGCT
CTCTCACAACATGGTGCTCATGGAAACAACCTCATTGCTGTTCTAGCCAAATACATCTAC
CACAAACATGACCCTGCTTTGCCACGTCTTGCCATTCAGCTGCTGAAACGTCTGGCCACG
GTGGCCCCAATGTCAGTGTATGCTTGTCTGGGCAATGATGCGGCTGCCATTCGTGATGCC
TTCCTGACCCGATTGCAGAGCAAAATTGAGGACATGCGCATCAAAGTCATGATTCTAGAG
TTCCTCACTGTTGCAGTAGAGACCCAGCCAGGCCTCATCGAACTGTTTCTGAACCTGGAA
GTTAAGGATGGCAGTGATGGCTCAAAGGAATTCAGCCTTGGGATGTGGAGCTGTCTCCAT
GCAGTGCTGGAGCTGATTGATTCCCAACAGCAAGATCGATACTGGTGCCCACCCCTGCTG
CATCGTGCCGCCATTGCCTTTTTGCATGCTCTGTGGCAGGATCGGAGGGACAGTGCCATG
CTGGTCCTCCGAACCAAACCCAAGTTTTGGGAAAATTTAACCAGTCCGCTGTTTGGAACC
CTTTCTCCTCCCTCTGAAACATCAGAGCCCAGCATCCTGGAAACCTGTGCCCTAATCATG
AAGATAATTTGCTTGGAGATATACTATGTAGTAAAGGGTTCATTAGACCAGTCATTAAAG
GATACACTGAAGAAATTTTCCATCGAGAAACGCTTTGCCTACTGGTCAGGGTATGTCAAG
TCATTGGCAGTTCACGTGGCCGAAACAGAAGGCAGCAGCTGCACCTCCTTGTTAGAGTAC
CAGATGCTGGTGTCCGCCTGGAGGATGCTTCTCATCATTGCCACCACTCATGCAGATATA
ATGCACCTGACTGACTCTGTGGTGCGTCGCCAGCTCTTTCTTGACGTGCTTGATGGAACC
AAAGCATTACTCCTAGTTCCAGCCTCAGTGAACTGCCTTCGCCTTGGCTCCATGAAGTGC
ACTCTGCTGCTTATCCTCCTCCGGCAGTGGAAGAGAGAGTTAGGTTCTGTGGATGAAATC
CTTGGACCCTTGACGGAGATCCTGGAGGGAGTGCTGCAGGCCGACCAGCAACTCATGGAG
AAGACCAAGGCCAAGGTGTTCTCAGCATTCATCACAGTGTTGCAAATGAAGGAGATGAAA
GTAAGTGACATCCCCCAGTACTCCCAGCTGGTGCTGAATGTCTGTGAGACCCTCCAAGAG
GAAGTGATTGCACTCTTCGACCAGACCCGCCACAGTCTGGCATTAGGCAGTGCCACAGAG
GACAAGGACAGCATGGAGACTGACGACTGTTCTCGGTCCCGGCACAGGGACCAGCGTGAT
GGGGTGTGTGTCCTGGGCCTGCACCTGGCCAAGGAGCTGTGTGAGGTAGACGAGGATGGT
GACTCCTGGCTGCAGGTAACCCGCAGGCTCCCCATCCTACCCACCCTCCTCACCACTCTA
GAGGTGAGCCTTCGCATGAAGCAGAACCTGCATTTCACTGAGGCCACATTGCATCTGCTC
CTCACCCTGGCTCGCACTCAGCAGGGAGCCACAGCAGTGGCTGGAGCTGGCATCACCCAG
AGCATTTGTTTGCCCCTTCTGAGTGTGTACCAGCTGAGCACCAACGGCACAGCACAGACA
CCTAGTGCCTCTCGGAAGTCCCTGGATGCCCCCTCTTGGCCAGGAGTCTACCGCCTGTCC
ATGTCCCTGATGGAGCAGCTGCTCAAAACTCTGCGCTACAACTTCCTGCCTGAGGCCCTG
GACTTCGTGGGTGTCCACCAGGAGCGGACCTTACAGTGCCTCAACGCAGTGAGGACAGTG
CAGAGTCTGGCCTGCCTGGAGGAGGCGGACCACACCGTGGGTTTTATTCTGCAGCTCTCT
AACTTCATGAAGGAGTGGCACTTCCACCTGCCTCAGCTCATGCGTGATATCCAGGTCAAC
CTGGGTTACTTGTGCCAGGCATGTACCTCTCTCCTGCACAGTCGAAAGATGCTGCAGCAT
TACTTACAGAACAAAAATGGGGATGGCCTCCCCTCAGCTGTTGCCCAGCGAGTCCAGAGG
CCACCGTCTGCTGCTTCTGCTGCCCCCTCCTCCTCAAAGCAGCCCGCTGCTGACACAGAG
GCATCAGAGCAGCAGGCCTTGCACACAGTCCAGTATGGCCTTCTCAAGATCCTCAGCAAG
ACGCTGGCAGCCCTGCGCCACTTCACCCCAGATGTCTGCCAGATTCTGCTGGATCAGTCC
CTGGACCTTGCTGAATACAACTTCCTGTTTGCCCTGAGCTTTACCACTCCCACCTTTGAC
TCCGAAGTGGCCCCCTCCTTCGGGACCCTTCTGGCCACAGTGAATGTGGCCCTCAACATG
CTTGGAGAGCTGGACAAGAAAAAGGAGCCCCTCACCCAGGCAGTGGGGCTCAGCACACAG
GCAGAAGGGACCAGGACGTTAAAGTCCCTCCTGATGTTTACCATGGAAAACTGCTTCTAC
CTGCTCATCTCTCAGGCGATGCGGTACCTTAGGGACCCGGCTGTGCACCCCCGGGACAAA
CAGCGGATGAAGCAGGAGCTCAGCTCTGAGTTGAGCACGCTGCTGTCCAGCCTCTCGCGC
TACTTCCGCCGGGGAGCCCCCAGCTCCCCTGCCACTGGTGTCCTCCCCTCGCCGCAGGGC
AAGTCCACCTCTCTCTCCAAAGCCAGCCCTGAGAGTCAGGAGCCTCTGATCCAGTTGGTG
CAGGCGTTTGTCCGGCATATGCAAAGA
Protein sequence predicted based on nucleotide sequence
MAAAAGGPCVRSSRELWTILLGRSALRELSQIEAELNKHWRRLLEGLSYYKPPSPSSAEK VKANKDVASPLKELGLRISKFLGLDEEQSVQLLQCYLQEDYRGTRDSVKTVLQDERQSQA LILKIADYYYEERTCILRCVLHLLTYFQDERHPYRVEYADCVDKLEKELVSKYRQQFEEL YKTEAPTWETHGNLMTERQVSRWFVQCLREQSMLLEIIFLYYAYFEMAPSDLLVLTKMFK EQGFGSRQTNRHLVDETMDPFVDRIGYFSALILVEGMDIESLHKCALDDRRELHQFAQDG LICQDMDCLMLTFGDIPHHAPVLLAWALLRHTLNPEETSSVVRKIGGTAIQLNVFQYLTR LLQSLASGGNDCTTSTACMCVYGLLSFVLTSLELHTLGNQQDIIDTACEVLADPSLPELF WGTEPTSGLGIILDSVCGMFPHLLSPLLQLLRALVSGKSTAKKVYSFLDKMSFYNELYKH KPHDVISHEDGTLWRRQTPKLLYPLGGQTNLRIPQGTVGQVMLDDRAYLVRWEYSYSSWT LFTCEIEMLLHVVSTADVIQHCQRVKPIIDLVHKVISTDLSIADCLLPITSRIYMLLQRL TTVISPPVDVIASCVNCLTVLAARNPAKVWTDLRHTGFLPFVAHPVSSLSQMISAEGMNA GGYGNLLMNSEQPQGEYGVTIAFLRLITTLVKGQLGSTQSQGLVPCVMFVLKEMLPSYHK WRYNSHGVREQIGCLILELIHAILNLCHETDLHSSHTPSLQFLCICSLAYTEAGQTVINI MGIGVDTIDMVMAAQPRSDGAEGQGQGQLLIKTVKLAFSVTNNVIRLKPPSNVVSPLEQA LSQHGAHGNNLIAVLAKYIYHKHDPALPRLAIQLLKRLATVAPMSVYACLGNDAAAIRDA FLTRLQSKIEDMRIKVMILEFLTVAVETQPGLIELFLNLEVKDGSDGSKEFSLGMWSCLH AVLELIDSQQQDRYWCPPLLHRAAIAFLHALWQDRRDSAMLVLRTKPKFWENLTSPLFGT LSPPSETSEPSILETCALIMKIICLEIYYVVKGSLDQSLKDTLKKFSIEKRFAYWSGYVK SLAVHVAETEGSSCTSLLEYQMLVSAWRMLLIIATTHADIMHLTDSVVRRQLFLDVLDGT KALLLVPASVNCLRLGSMKCTLLLILLRQWKRELGSVDEILGPLTEILEGVLQADQQLME KTKAKVFSAFITVLQMKEMKVSDIPQYSQLVLNVCETLQEEVIALFDQTRHSLALGSATE DKDSMETDDCSRSRHRDQRDGVCVLGLHLAKELCEVDEDGDSWLQVTRRLPILPTLLTTL EVSLRMKQNLHFTEATLHLLLTLARTQQGATAVAGAGITQSICLPLLSVYQLSTNGTAQT PSASRKSLDAPSWPGVYRLSMSLMEQLLKTLRYNFLPEALDFVGVHQERTLQCLNAVRTV QSLACLEEADHTVGFILQLSNFMKEWHFHLPQLMRDIQVNLGYLCQACTSLLHSRKMLQH YLQNKNGDGLPSAVAQRVQRPPSAASAAPSSSKQPAADTEASEQQALHTVQYGLLKILSK TLAALRHFTPDVCQILLDQSLDLAEYNFLFALSFTTPTFDSEVAPSFGTLLATVNVALNM LGELDKKKEPLTQAVGLSTQAEGTRTLKSLLMFTMENCFYLLISQAMRYLRDPAVHPRDK QRMKQELSSELSTLLSSLSRYFRRGAPSSPATGVLPSPQGKSTSLSKASPESQEPLIQLV QAFVRHMQR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
23
End on reference sequence
5272
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
