Gene:
Gene Symbol:
FMNL3
Gene Name:
formin-like 3
Original Clone ID:
FLH481802.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295460
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGGCAACCTGGAGAGCGCCGAGGG
GGTCCCGGGAGAGCCCCCCTCTGTCCCGTTGTTGCTGCCGCCCGGCAAGATGCCGATGCC
TGAGCCCTGTGAGCTGGAGGAAAGGTTCGCCCTGGTGCTGAGCTCCATGAACCTGCCTCC
AGACAAGGCCCGGCTCCTGCGGCAGTATGACAATGAGAAGAAATGGGATCTGATCTGTGA
CCAGGAACGATTCCAGGTGAAGAATCCTCCCCACACTTACATTCAGAAACTCCAGAGCTT
CTTGGACCCCAGTGTAACTCGGAAGAAGTTCAGGAGGAGGGTGCAGGAGTCAACCAAAGT
ACTAAGGGAGCTGGAGATCTCTCTTCGCACCAACCACATTGGGTGGGTGCGGGAATTTCT
GAATGATGAAAACAAAGGCCTGGATGTACTGGTGGATTACCTGTCCTTTGCCCAGTGTTC
TGTCATGTTTGACTTTGAGGGTCTGGAAAGTGGTGACGATGGTGCATTTGACAAACTCCG
GTCCTGGAGCAGGTCAATCGAGGACCTGCAGCCACCCAGCGCCCTGTCGGCCCCCTTCAC
CAACAGCCTCGCTCGCTCTGCGCGCCAGTCTGTGCTCCGGTATAGCACTCTCCCTGGGCG
CAGGGCCCTGAAGAACTCCCGCCTAGTGAGCCAGAAGGATGACGTCCACGTCTGTATCCT
TTGTCTCAGAGCCATCATGAACTATCAGTACGGATTCAACCTGGTCATGTCCCACCCCCA
TGCTGTCAATGAGATTGCACTTAGCCTCAATAACAAGAATCCAAGGACCAAAGCCCTTGT
CTTAGAGCTTCTGGCAGCTGTGTGTTTGGTGCGAGGAGGTCACGAAATCATCCTTGCTGC
CTTTGACAATTTCAAAGAGGTATGCAAGGAGCTGCACCGCTTTGAGAAGCTGATGGAGTA
TTTCCGGAATGAGGACAGCAATATTGACTTCATGGTGGCCTGCATGCAGTTCATCAACAT
CGTGGTGCACTCGGTGGAGGACATGAACTTCCGGGTCCACCTGCAGTATGAGTTTACCAA
GCTGGGGCTAGAGGAGTTCCTGCAGAAGTCAAGGCACACAGAGAGCGAGAAGCTGCAGGT
GCAGATTCAGGCATATCTGGACAACGTGTTTGATGTCGGGGGTTTGTTGGAGGATGCTGA
GACCAAGAATGTAGCCCTGGAGAAGGTGGAGGAGTTGGAGGAGCATGTGTCCCATCTCAC
AGAGAAGCTTCTGGACCTAGAGAATGAAAACATGATGCGGGTGGCAGAACTAGAGAAGCA
GCTGCTACAGCGGGAGAAGGAACTAGAGAGCATCAAGGAGACATATGAGAACACAAGCCA
CCAGGTGCACACCCTGCGGAGGCTCATTAAAGAGAAGGAGGAGGCCTTTCAGCGTCGATG
TCATTTGGAGCCAAATGTCCGGGGCCTGGAGTCTGTGGACAGTGAGGCCCTGGCCAGAGT
AGGCCCTGCAGAGCTGAGTGAGGGCATGCCACCCTCCGACCTGGACCTTCTGGCTCCAGC
CCCACCCCCTGAGGAGGTCCTGCCTCTTCCTCCACCACCAGCTCCGCCCTTGCCCCCTCC
ACCTCCCCCATTACCAGACAAGTGTCCCCCAGCCCCACCTCTCCCTGGTGCTGCACCCTC
TGTGGTGTTGACAGTGGGCCTGTCAGCCATTCGAATTAAGAAACCTATCAAGACCAAGTT
CCGGCTGCCTGTCTTCAACTGGACAGCACTGAAACCCAACCAGATCAGTGGCACTGTCTT
CAGCGAACTTGATGATGAGAAGATCTTGGAGGACCTGGATCTTGATAAGTTTGAAGAATT
ATTCAAGACAAAAGCGCAGGGCCCTGCCCTTGACCTCATCTGCTCCAAAAACAAGACAGC
GCAAAAAGCTGCCAGCAAGGTGACTCTGTTGGAAGCCAATCGTGCCAAGAACCTGGCCAT
CACCCTACGCAAGGCTGGCCGCTCGGCTGAGGAGATCTGCAGGGCCATTCATACGTTTGA
CTTGCAGACACTACCTGTGGACTTCGTGGAGTGCCTGATGCGCTTCCTGCCCACAGAGGC
TGAGGTAAAGCTGCTGCGGCAATATGAGCGGGAGCGGCAGCCCCTGGAGGAGTTGGCAGC
TGAGGACCGCTTCATGCTGCTCTTCAGCAAGGTGGAACGGTTGACCCAGCGAATGGCTGG
CATGGCCTTCCTGGGGAACTTCCAGGATAACCTGCAGATGCTCACACCGCAACTCAATGC
CATCATTGCGGCGTCCGCTTCCGTCAAGTCTTCACAGAAGCTGAAGCAGATGTTGGAGAT
CATACTTGCACTGGGGAACTACATGAACAGCAGCAAGCGGGGAGCTGTGTATGGCTTCAA
GCTCCAGAGCCTGGATCTGCTGCTGGATACCAAGTCCACTGACCGGAAGATGACACTGCT
TCATTTCATCGCCTTGACAGTGAAGGAGAAATACCCAGACCTGGCTAACTTCTGGCATGA
GCTGCACTTTGTTGAGAAGGCTGCAGCAGTGTCCCTGGAGAACGTGCTGCTGGACGTGAA
GGAGCTGGGCCGGGGCATGGAGCTGATTCGGCGTGAGTGCAGCATCCATGACAACAGCGT
CCTCCGGAACTTCCTCAGTACCAATGAAGGCAAACTAGACAAGCTCCAGCGGGACGCCAA
GACGGCTGAGGAGGCCTACAATGCAGTTGTGCGCTACTTTGGCGAGAGTCCCAAGACTAC
ACCTCCTTCTGTATTCTTCCCAGTATTTGTCCGATTCATTCGTTCTTACAAGGAAGCAGA
ACAAGAGAATGAAGCCCGCAAGAAGCAGGAGGAGGTAATGCGGGAGAAGCAGCTGGCTCA
GGAAGCCAAGAAACTGGATGCCAAGACCCCATCCCAGCGGAACAAGTGGCAACAGCAGGA
GTTAATAGCAGAGTTGAGGCGGCGCCAGGCCAAGGAACACCGGCCTGTTTATGAGGGGAA
GGATGGTACCATCGAGGACATCATCACAGGCCTCCACTGCCAGCCTATGGTTGTTCGCCA
CCAAGCCAGGAGTGCTGCACCGCCCAGTGGTCCCCCTCGGGCTCCAGGCCCCCACTAGGG
CCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MGNLESAEGVPGEPPSVPLLLPPGKMPMPEPCELEERFALVLSSMNLPPDKARLLRQYDN EKKWDLICDQERFQVKNPPHTYIQKLQSFLDPSVTRKKFRRRVQESTKVLRELEISLRTN HIGWVREFLNDENKGLDVLVDYLSFAQCSVMFDFEGLESGDDGAFDKLRSWSRSIEDLQP PSALSAPFTNSLARSARQSVLRYSTLPGRRALKNSRLVSQKDDVHVCILCLRAIMNYQYG FNLVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHEIILAAFDNFKEVCKEL HRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVEDMNFRVHLQYEFTKLGLEEFLQKSR HTESEKLQVQIQAYLDNVFDVGGLLEDAETKNVALEKVEELEEHVSHLTEKLLDLENENM MRVAELEKQLLQREKELESIKETYENTSHQVHTLRRLIKEKEEAFQRRCHLEPNVRGLES VDSEALARVGPAELSEGMPPSDLDLLAPAPPPEEVLPLPPPPAPPLPPPPPPLPDKCPPA PPLPGAAPSVVLTVGLSAIRIKKPIKTKFRLPVFNWTALKPNQISGTVFSELDDEKILED LDLDKFEELFKTKAQGPALDLICSKNKTAQKAASKVTLLEANRAKNLAITLRKAGRSAEE ICRAIHTFDLQTLPVDFVECLMRFLPTEAEVKLLRQYERERQPLEELAAEDRFMLLFSKV ERLTQRMAGMAFLGNFQDNLQMLTPQLNAIIAASASVKSSQKLKQMLEIILALGNYMNSS KRGAVYGFKLQSLDLLLDTKSTDRKMTLLHFIALTVKEKYPDLANFWHELHFVEKAAAVS LENVLLDVKELGRGMELIRRECSIHDNSVLRNFLSTNEGKLDKLQRDAKTAEEAYNAVVR YFGESPKTTPPSVFFPVFVRFIRSYKEAEQENEARKKQEEVMREKQLAQEAKKLDAKTPS QRNKWQQQELIAELRRRQAKEHRPVYEGKDGTIEDIITGLHCQPMVVRHQARSAAPPSGP PRAPGPH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
235
End on reference sequence
3318
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
