Gene:
Gene Symbol:
TGS1
Gene Name:
trimethylguanosine synthase 1
Original Clone ID:
FLH481808.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295466
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTGCTGCGAGAAGTGGAGCCGCGTGGCGGAAATGTTTCTCTTCATTGAGGAGCGGGAG
GATTGTAAGATACTGTGCCTTTGCTCCAGGGCATTTGTGGAGGATCGAAAATTGTACAAT
TTGGGATTAAAAGGCTATTACATCAGAGACAGTGGCAACAATTCAGGAGACCAGGCGACA
GAAGAAGAGGAAGGTGGTTATTCCTGTGGTACTGCAGAATCACATGACAGCAAAGGCATA
GGCCTGGATGAAAGTGAACTTGATTCTGAGGCTGAACTCATGAGAAGTATGGGATTGCCA
CTTCAATTTGGTAGGATAACTGCACATAAGGATTTTGAGGTATCTATGAATACTAGAAAT
AAAGTTAAAATAAAAAAGAAAAAACATCAAAAGAAATACTTAGATGAAATTGTGCAAGAA
TCTTGGAGAAAAGAATATGAAGAAGACGACATTTTGGCTTCAGATGATCCATCTTCAATT
GAACAGTATGAGAACACCAGAACATATGAACTTCAAAGCAAAAAAGATACTGAGACAGAA
AATCCTCCAGTTGAAAACACATTATCTCCAAAGCTAGAAATTACAGAGAAATGGGAAAAG
TATTGGAATGAATATGGAGGAGGACTATTGTGGCAAAGTTGGCAAGAAAAACATCCGGGT
CAAGCACTATCTTCTGAACCTTGGAACTTTCCTGATACAAAGGAAGAATGGGAGCAACAT
TATAGTCAACTTTATTGGTATTATTTGGAACAATTTCAGTATTGGGAAGCTCAGGGTTGG
ACTTTTGATGCCTCGCAAAGCTGTGATACAGATACTTACACATCTAAAACAGAAGCTGAT
GACAAGAACGATGAAAAATGCATGAAAGTTGACTTAGTATCTTTTCCATCTTCACCTATT
ATGGTTGATAATGATAGCTCTGGTACAAGTGATAAGGATCATAGTGAAATACTTGATGGA
ATTAGTAACATAAAACTGAATTCAGAGGAAGTAACACAGAGCCAATTAGATTCCTGTACA
AGTCATGATGGTCATCAACAGCTAAGTGAAGTTAGTAGCAAAAGAGAGTGCCCTGCTTCC
GGCCAAAGTGAACCACGTAATGGAGGAACCAATGAGGAAAGCAACTCATCGGGGAATACA
AACACAGACCCACCAGCTGAGGATTCACAGAAGTCTTCAGGAGCAAACACAAGCAAAGAC
AGACCACATGCCAGTGGTACTGATGGAGATGAAAGTGAGGAAGACCCACCTGAGCATAAG
CCAAGCAAACTGAAGAGGAGCCATGAACTGGACATTGATGAAAACCCAGCTTCAGACTTT
GATGACAGTGGTTCCCTTCTAGGATTCAAGTATGGCTCAGGACAAAAATATGGTGGAATC
CCAAATTTCAGTCATCGGCAGGTCAGGTATTTAGAGAAGAATGTGAAGCTTAAGTCTAAG
TACCTAGACATGCGCAGACAAATAAAGATGAAAAACAAACACATCTTCTTTACCAAAGAG
TCAGAAAAACCATTTTTCAAGAAAAGCAAAATTCTGAGTAAGGTAGAAAAATTCCTCACA
TGGGTTAATAAACCAATGGATGAAGAAGCATCACAGGAATCATCTTCTCATGACAATGTG
CACGACGCTTCCACAAGTAGTGATTCAGAGGAACAAGACATGTCTGTTAAAAAAGGTGAT
GACCTACTGGAGACTAATAATCCAGAACCTGAAAAGTGTCAGAGCGTATCTTCAGCTGGT
GAACTTGAAACAGAAAACTATGAAAGAGACAGCTTGCTAGCAACTGTTCCAGATGAGCAG
GATTGTGTTACTCAAGAAGTGCCAGACTCCCGCCAGGCAGAAACTGAAGCTGAAGTGAAA
AAGAAGAAGAACAAGAAGAAGAACAAAAAGGTGAATGGTCTGCCTCCTGAAATAGCTGCT
GTTCCTGAGCTGGCAAAATACTGGGCCCAGAGGTACAGGCTCTTCTCCCGTTTTGATGAT
GGGATTAAGTTGGACAGAGAGGGCTGGTTTTCAGTTACACCCGAGAAGATTGCTGAACAC
ATTGCTGGCCGTGTTAGTCAGTCCTTCAAGTGTGACGTTGTAGTAGACGCATTCTGTGGA
GTTGGAGGAAATACCATTCAGTTTGCCTTAACAGGAATGAGAGTGATTGCCATTGATATC
GATCCTGTTAAGATTGCCCTTGCTCGCAATAATGCAGAAGTTTATGGGATAGCAGATAAG
ATAGAGTTCATCTGTGGAGATTTCTTGCTGCTGGCTTCTTTTTTAAAGGCTGATGTTGTG
TTCCTCAGCCCACCTTGGGGAGGGCCAGACTATGCCACTGCAGAGACCTTTGACATTAGA
ACAATGATGTCTCCTGATGGCTTTGAAATTTTCAGACTTTCTAAGAAGATCACTAATAAT
ATTGTTTATTTTCTTCCAAGAAATGCTGATATTGACCAGGTGGCATCCTTAGCTGGGCCT
GGAGGGCAAGTGGAAATAGAACAGAACTTCCTTAACAACAAATTGAAGACAATCACTGCA
TATTTTGGTGACCTAATTCGAAGACCAGCCTCTGAAACC
Protein sequence predicted based on nucleotide sequence
MCCEKWSRVAEMFLFIEEREDCKILCLCSRAFVEDRKLYNLGLKGYYIRDSGNNSGDQAT EEEEGGYSCGTAESHDSKGIGLDESELDSEAELMRSMGLPLQFGRITAHKDFEVSMNTRN KVKIKKKKHQKKYLDEIVQESWRKEYEEDDILASDDPSSIEQYENTRTYELQSKKDTETE NPPVENTLSPKLEITEKWEKYWNEYGGGLLWQSWQEKHPGQALSSEPWNFPDTKEEWEQH YSQLYWYYLEQFQYWEAQGWTFDASQSCDTDTYTSKTEADDKNDEKCMKVDLVSFPSSPI MVDNDSSGTSDKDHSEILDGISNIKLNSEEVTQSQLDSCTSHDGHQQLSEVSSKRECPAS GQSEPRNGGTNEESNSSGNTNTDPPAEDSQKSSGANTSKDRPHASGTDGDESEEDPPEHK PSKLKRSHELDIDENPASDFDDSGSLLGFKYGSGQKYGGIPNFSHRQVRYLEKNVKLKSK YLDMRRQIKMKNKHIFFTKESEKPFFKKSKILSKVEKFLTWVNKPMDEEASQESSSHDNV HDASTSSDSEEQDMSVKKGDDLLETNNPEPEKCQSVSSAGELETENYERDSLLATVPDEQ DCVTQEVPDSRQAETEAEVKKKKNKKKNKKVNGLPPEIAAVPELAKYWAQRYRLFSRFDD GIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGGNTIQFALTGMRVIAIDI DPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIR TMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITA YFGDLIRRPASET
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2949
Start on reference sequence
388
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
