Gene:
Gene Symbol:
CNTNAP1
Gene Name:
contactin associated protein 1
Original Clone ID:
FLH481835.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295493
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATGCATCTCCGGCTCTTCTGCATCCTGCTCGCCGCGGTCTCAGGAGCCGAGGGCTGG
GGCTACTACGGCTGCGACGAGGAGCTGGTGGGTCCCCTGTATGCACGCTCCCTGGGCGCC
TCCTCCTACTACAGTCTCCTTACTGCGCCGCGATTCGCCAGGCTGCACGGCATAAGCGGG
TGGTCACCACGGATTGGGGATCCGAATCCCTGGCTCCAGATAGACTTAATGAAGAAGCAC
CGGATCCGGGCCGTGGCCACACAGGGCTCCTTTAATTCTTGGGACTGGGTCACACGTTAC
ATGCTACTCTACGGCGACCGAGTGGACAGCTGGACACCGTTCTACCAGCGAGGGCACAAC
TCGACCTTCTTTGGTAACGTGAACGAGTCGGCGGTGGTGCGCCATGACCTGCACTTCCAC
TTCACTGCGCGCTACATCCGCATCGTGCCCCTGGCCTGGAACCCACGCGGCAAGATCGGC
CTGAGGCTCGGCCTCTATGGCTGCCCATACAAGGCCGACATACTCTATTTCGACGGCGAC
GATGCCATCTCCTACCGCTTCCCGCGAGGGGTCAGCCGAAGCCTGTGGGACGTGTTCGCC
TTCAGCTTCAAGACCGAGGAGAAGGACGGTCTTCTGCTGCACGCCGAGGGCGCCCAGGGC
GACTACGTGACGCTCGAGCTGGAGGGGGCACACCTGCTGCTGCACATGAGCCTGGGCAGC
AGCCCTATCCAGCCAAGACCAGGTCACACCACCGTGAGCGCAGGCGGAGTCCTCAATGAC
CAGCACTGGCACTATGTGCGGGTGGACCGATTTGGCCGCGATGTAAATTTCACCCTGGAC
GGCTATGTGCAGCGCTTTATTCTCAATGGAGACTTCGAGAGGCTGAACCTGGACACTGAG
ATGTTCATCGGAGGTCTGGTGGGCGCCGCGCGGAAGAACCTGGCCTATCGGCATAACTTC
CGCGGCTGCATAGAAAACGTAATCTTCAACCGCGTCAACATCGCAGACCTGGCCGTGCGG
CGCCATTCCCGGATCACCTTCGAGGGTAAGGTGGCTTTTCGTTGCCTGGACCCGGTACCG
CACCCTATCAACTTCGGAGGCCCTCACAACTTCGTTCAAGTGCCCGGTTTCCCACGCCGT
GGCCGCCTGGCAGTCTCATTTCGCTTCCGCACCTGGGACCTCACCGGGCTTCTCCTTTTC
TCCCGTCTGGGGGACGGGCTGGGCCACGTGGAGCTGACGCTCAGCGAAGGGCAGGTCAAC
GTGTCCATCGCGCAGAGCGGCCGAAAGAAGCTTCAGTTCGCTGCTGGGTACCGACTGAAT
GACGGCTTTTGGCACGAGGTGAATTTTGTGGCACAGGAAAACCATGCAGTTATCAGCATT
GATGATGTGGAAGGGGCAGAGGTCAGGGTCTCATACCCGTTGCTGATCCGGACAGGGACC
TCATATTTCTTTGGGGGTTGTCCCAAGCCAGCCAGTCGATGGGACTGCCACTCCAACCAG
ACGGCATTCCATGGCTGCATGGAGCTGCTCAAGGTGGATGGTCAACTGGTCAACCTGACT
CTGGTGGAGGGCCGGCGGCTTGGATTCTATGCTGAGGTCCTCTTTGATACATGTGGCATC
ACTGATAGGTGCAGCCCTAACATGTGTGAGCATGATGGACGCTGCTACCAGTCTTGGGAT
GACTTCATTTGCTACTGCGAACTGACGGGCTACAAGGGAGAGACCTGCCACACACCTTTG
TATAAGGAATCCTGTGAGGCTTATCGGCTCAGTGGGAAAACTTCTGGAAACTTCACCATT
GATCCTGATGGCAGTGGCCCCCTGAAGCCATTTGTAGTGTACTGTGATATCCGAGAGAAC
CGAGCGTGGACAGTTGTGCGGCATGACAGGCTGTGGACAACTCGAGTGACAGGTTCCAGC
ATGGAGCGGCCATTCCTGGGGGCTATCCAGTACTGGAATGCATCCTGGGAGGAAGTCAGT
GCCCTTGCCAATGCTTCCCAGCATTGTGAACAGTGGATCGAGTTCTCCTGCTACAATTCC
CGGCTGCTCAACACTGCAGGAGGCTACCCCTACAGCTTTTGGATTGGCCGAAATGAGGAG
CAGCACTTCTACTGGGGAGGCTCCCAGCCTGGGATCCAGCGCTGTGCCTGTGGTCTGGAC
CGGAGCTGTGTGGACCCTGCCTTGTACTGCAACTGTGACGCTGACCAGCCCCAGTGGAGA
ACTGACAAGGGACTGCTGACCTTTGTGGACCATCTGCCTGTCACTCAGGTAGTGATAGGG
GATACGAACCGCTCCACTTCTGAGGCCCAGTTCTTCCTGAGGCCTCTGCGCTGCTATGGC
GATCGAAATTCCTGGAACACCATTTCCTTCCACACCGGGGCTGCACTACGCTTCCCCCCA
ATCCGTGCCAACCACAGCCTGGATGTCTCCTTCTACTTCAGGACCTCTGCTCCCTCGGGG
GTCTTCCTAGAGAATATGGGGGGCCCTTACTGCCAGTGGCGCCGACCTTATGTGCGGGTG
GAACTCAACACATCCCGGGATGTGGTCTTCGCCTTTGATGTGGGGAATGGGGATGAGAAC
CTCACAGTACACTCAGACGACTTTGAGTTCAATGATGACGAGTGGCACCTGGTCCGGGCT
GAAATCAACGTGAAGCAGGCCCGGCTCCGAGTGGATCACCGGCCCTGGGTTCTGCGGCCT
ATGCCACTGCAGACCTACATCTGGATGGAGTATGACCAGCCCCTCTATGTGGGATCTGCA
GAGCTTAAGAGACGCCCCTTTGTGGGTTGCTTGAGGGCCATGCGTCTGAACGGAGTGACT
CTGAACCTGGAGGGCCGTGCCAATGCCTCTGAGGGTACCTCACCCAACTGCACAGGCCAC
TGTGCCCACCCTCGGCTCCCCTGTTTCCATGGAGGCCGCTGCGTGGAGCGCTATAGCTAC
TACACGTGTGACTGTGACCTCACGGCTTTTGATGGGCCATACTGCAACCACGATATTGGT
GGTTTCTTTGAGCCGGGCACCTGGATGCGCTATAACCTACAGTCAGCGCTGCGCTCTGCA
GCCAGGGAGTTCTCCCACATGCTGAGCCGGCCAGTGCCAGGCTATGAGCCTGGCTACATC
CCGGGCTATGATACTCCGGGCTATGTGCCTGGCTACCATGGCCCCGGGTACCGCCTGCCC
GACTACCCCCGGCCTGGTCGGCCTGTGCCCGGTTACCGTGGGCCTGTCTACAACGTTACG
GGAGAGGAGGTCTCCTTCAGCTTCAGCACCAGCTCCGCCCCTGCTGTCCTGCTCTACGTC
AGTTCCTTTGTTCGTGACTACATGGCTGTGCTCATCAAGGATGATGGGACCCTTCAGCTG
CGATATCAGCTGGGCACCAGTCCCTACGTGTACCAGCTAACCACTCGACCAGTGACCGAT
GGCCAGCCCCATAGCATCAATATCACCCGTGTTTACCGGAACCTCTTCATCCAGGTGGAC
TACTTCCCACTGACAGAGCAGAAGTTCTCGCTGTTGGTGGACAGCCAGTTGGACTCACCC
AAGGCCTTGTATTTAGGGCGTGTGATGGAGACAGGAGTCATTGACCCGGAGATCCAGCGC
TACAACACCCCAGGTTTCTCAGGCTGCCTGTCTGGTGTTCGATTCAACAACGTGGCTCCC
CTCAAGACCCACTTCCGAACCCCTCGACCCATGACTGCTGAGCTAGCTGAGGCCCTTCGA
GTTCAGGGAGAACTGTCCGAATCTAATTGCGGAGCTATGCCACGTCTTGTTTCAGAGGTG
CCACCTGAGCTTGATCCCTGGTATCTGCCCCCAGACTTCCCCTACTACCATGATGAAGGA
TGGGTTGCCATACTTTTAGGCTTTTTGGTGGCCTTTCTGCTGCTGGGGCTGGTGGGAATG
TTGGTGCTCTTCTATCTGCAAAATCATCGCTATAAGGGCTCCTACCATACCAATGAGCCC
AAGGCTGCCCACGAGTACCATCCTGGCAGCAAACCTCCCCTACCCACTTCAGGCCCTGCC
CAGGTCCCCACCCCTACAGCAGCTCCCAACCAAGCTCCAGCCTCAGCCCCAGCCCCAGCC
CCAACTCCAGCCCCAGCCCCTGGCCCCCGGGATCAGAACCTACCCCAGATCCTGGAGGAG
TCCAGGTCTGAA
Protein sequence predicted based on nucleotide sequence
MMHLRLFCILLAAVSGAEGWGYYGCDEELVGPLYARSLGASSYYSLLTAPRFARLHGISG WSPRIGDPNPWLQIDLMKKHRIRAVATQGSFNSWDWVTRYMLLYGDRVDSWTPFYQRGHN STFFGNVNESAVVRHDLHFHFTARYIRIVPLAWNPRGKIGLRLGLYGCPYKADILYFDGD DAISYRFPRGVSRSLWDVFAFSFKTEEKDGLLLHAEGAQGDYVTLELEGAHLLLHMSLGS SPIQPRPGHTTVSAGGVLNDQHWHYVRVDRFGRDVNFTLDGYVQRFILNGDFERLNLDTE MFIGGLVGAARKNLAYRHNFRGCIENVIFNRVNIADLAVRRHSRITFEGKVAFRCLDPVP HPINFGGPHNFVQVPGFPRRGRLAVSFRFRTWDLTGLLLFSRLGDGLGHVELTLSEGQVN VSIAQSGRKKLQFAAGYRLNDGFWHEVNFVAQENHAVISIDDVEGAEVRVSYPLLIRTGT SYFFGGCPKPASRWDCHSNQTAFHGCMELLKVDGQLVNLTLVEGRRLGFYAEVLFDTCGI TDRCSPNMCEHDGRCYQSWDDFICYCELTGYKGETCHTPLYKESCEAYRLSGKTSGNFTI DPDGSGPLKPFVVYCDIRENRAWTVVRHDRLWTTRVTGSSMERPFLGAIQYWNASWEEVS ALANASQHCEQWIEFSCYNSRLLNTAGGYPYSFWIGRNEEQHFYWGGSQPGIQRCACGLD RSCVDPALYCNCDADQPQWRTDKGLLTFVDHLPVTQVVIGDTNRSTSEAQFFLRPLRCYG DRNSWNTISFHTGAALRFPPIRANHSLDVSFYFRTSAPSGVFLENMGGPYCQWRRPYVRV ELNTSRDVVFAFDVGNGDENLTVHSDDFEFNDDEWHLVRAEINVKQARLRVDHRPWVLRP MPLQTYIWMEYDQPLYVGSAELKRRPFVGCLRAMRLNGVTLNLEGRANASEGTSPNCTGH CAHPRLPCFHGGRCVERYSYYTCDCDLTAFDGPYCNHDIGGFFEPGTWMRYNLQSALRSA AREFSHMLSRPVPGYEPGYIPGYDTPGYVPGYHGPGYRLPDYPRPGRPVPGYRGPVYNVT GEEVSFSFSTSSAPAVLLYVSSFVRDYMAVLIKDDGTLQLRYQLGTSPYVYQLTTRPVTD GQPHSINITRVYRNLFIQVDYFPLTEQKFSLLVDSQLDSPKALYLGRVMETGVIDPEIQR YNTPGFSGCLSGVRFNNVAPLKTHFRTPRPMTAELAEALRVQGELSESNCGAMPRLVSEV PPELDPWYLPPDFPYYHDEGWVAILLGFLVAFLLLGLVGMLVLFYLQNHRYKGSYHTNEP KAAHEYHPGSKPPLPTSGPAQVPTPTAAPNQAPASAPAPAPTPAPAPGPRDQNLPQILEE SRSE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
218
End on reference sequence
4372
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
