DNASU Plasmid | ZBTB34 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZBTB34

Gene Name: zinc finger and BTB domain containing 34

Original Clone ID:

FLH481880.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00295538

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1500nts

Open reading frame: 1 to 1500

Reference Sequence Alignment

ATGGACAGCAGCAGTTTTATTCAGTTTGATGTGCCCGAGTACAGCAGCACCGTTCTGAGC
CAGCTAAACGAACTCCGCCTGCAGGGGAAACTATGTGACATCATTGTACACATTCAGGGT
CAGCCATTCCGAGCCCACAAAGCAGTCCTTGCTGCCAGCTCCCCATATTTCCGGGACCAT
TCAGCGTTAAGTACCATGAGTGGCTTGTCAATATCAGTGATTAAAAATCCCAATGTGTTT
GAGCAGTTGCTTTCTTTTTGTTACACTGGAAGAATGTCCTTGCAGCTGAAGGATGTTGTC
AGTTTTCTGACTGCAGCCAGCTTTCTTCAGATGCAGTGTGTCATTGACAAGTGCACGCAG
ATCCTAGAGAGCATCCATTCCAAAATCAGCGTTGGAGATGTTGACTCTGTTACCGTCGGT
GCTGAAGAGAATCCCGAGAGTCGAAACGGAGTGAAAGACAGCAGCTTCTTTGCCAACCCA
GTGGAGATCTCTCCTCCATATTGCTCTCAGGGACGGCAGCCCACCGCAAGCAGTGACCTC
CGGATGGAGACGACCCCCAGCAAAGCTTTGCGCAGCCGCTTACAGGAGGAGGGGCACTCA
GACCGCGGGAGCAGTGGGAGCGTTTCTGAATATGAGATTCAGATAGAGGGAGACCATGAG
CAAGGAGACCTATTGGTGAGGGAGAGCCAGATCACCGAGGTGAAAGTGAAGATGGAGAAG
TCCGACCGGCCCAGCTGTTCCGACAGCTCCTCCCTGGGTGACGATGGGTACCACACCGAG
ATGGTTGATGGGGAACAAGTTGTGGCAGTGAATGTGGGCTCCTATGGTTCTGTGCTCCAG
CACGCATACTCCTATTCCCAAGCAGCCTCACAGCCAACCAATGTATCAGAAGCTTTTGGA
AGTTTGAGTAATTCCAGCCCATCCAGGTCCATGCTGAGCTGTTTCCGAGGAGGGCGTGCC
CGCCAGAAGCGGGCTTTGTCTGTCCACCTGCACAGTGACCTGCAGGGCCTGGTGCAGGGC
TCTGACAGTGAAGCCATGATGAACAACCCCGGGTATGAGAGCAGTCCCCGGGAGAGGAGT
GCGAGAGGGCATTGGTACCCGTACAATGAGAGGTTGATCTGTATTTACTGTGGAAAGTCC
TTCAACCAGAAAGGAAGCCTTGATAGGCACATGCGACTCCATATGGGAATCACCCCCTTT
GTGTGCAAGTTCTGTGGGAAGAAGTACACACGGAAGGACCAACTGGAGTACCACATCCGG
GGCCATACAGATGATAAACCATTCCGCTGTGAGATCTGCGGGAAGTGCTTTCCATTCCAA
GGTACCCTCAACCAGCACTTGCGGAAAAACCACCCAGGCGTTGCTGAAGTCAGGAGTCGC
ATTGAGTCCCCCGAGAGAACAGATGTGTACGTGGAACAGAAACTAGAAAATGACGCATCG
GCCTCAGAGATGGGCCTAGATTCCCGGATGGAAATTCACACAGTGTCTGATGCTCCCGAT

Protein sequence predicted based on nucleotide sequence

MDSSSFIQFDVPEYSSTVLSQLNELRLQGKLCDIIVHIQGQPFRAHKAVLAASSPYFRDH
SALSTMSGLSISVIKNPNVFEQLLSFCYTGRMSLQLKDVVSFLTAASFLQMQCVIDKCTQ
ILESIHSKISVGDVDSVTVGAEENPESRNGVKDSSFFANPVEISPPYCSQGRQPTASSDL
RMETTPSKALRSRLQEEGHSDRGSSGSVSEYEIQIEGDHEQGDLLVRESQITEVKVKMEK
SDRPSCSDSSSLGDDGYHTEMVDGEQVVAVNVGSYGSVLQHAYSYSQAASQPTNVSEAFG
SLSNSSPSRSMLSCFRGGRARQKRALSVHLHSDLQGLVQGSDSEAMMNNPGYESSPRERS
ARGHWYPYNERLICIYCGKSFNQKGSLDRHMRLHMGITPFVCKFCGKKYTRKDQLEYHIR
GHTDDKPFRCEICGKCFPFQGTLNQHLRKNHPGVAEVRSRIESPERTDVYVEQKLENDAS
ASEMGLDSRMEIHTVSDAPD

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1600

Start on reference sequence 98

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

ZBTB34

Symbol Nomenclature :

ZBTB34

SYNONYM :

RP11-106H5.1; ZNF918

Designation :

zinc finger and BTB domain-containing protein 34

Full Nomenclature :

zinc finger and BTB domain containing 34

GENEID :

403341

GI :

182888236

GenBank Accession :

BC160177

HGNC :

31446

MIM :

611692

Vega :

OTTHUMG00000020694

Target GenBank:

NM_001099270.1

Reference Sequence Alignment

  

HsCD00295538        GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACAGCAGCAGTTTTATTCAGTT
NM_001099270.1      ----------------------------------ATGGACAGCAGCAGTTTTATTCAGTT
                                                      **************************

HsCD00295538        TGATGTGCCCGAGTACAGCAGCACCGTTCTGAGCCAGCTAAACGAACTCCGCCTGCAGGG
NM_001099270.1      TGATGTGCCCGAGTACAGCAGCACCGTTCTGAGCCAGCTAAACGAACTCCGCCTGCAGGG
                    ************************************************************

HsCD00295538        GAAACTATGTGACATCATTGTACACATTCAGGGTCAGCCATTCCGAGCCCACAAAGCAGT
NM_001099270.1      GAAACTATGTGACATCATTGTACACATTCAGGGTCAGCCATTCCGAGCCCACAAAGCAGT
                    ************************************************************

HsCD00295538        CCTTGCTGCCAGCTCCCCATATTTCCGGGACCATTCAGCGTTAAGTACCATGAGTGGCTT
NM_001099270.1      CCTTGCTGCCAGCTCCCCATATTTCCGGGACCATTCAGCGTTAAGTACCATGAGTGGCTT
                    ************************************************************

HsCD00295538        GTCAATATCAGTGATTAAAAATCCCAATGTGTTTGAGCAGTTGCTTTCTTTTTGTTACAC
NM_001099270.1      GTCAATATCAGTGATTAAAAATCCCAATGTGTTTGAGCAGTTGCTTTCTTTTTGTTACAC
                    ************************************************************

HsCD00295538        TGGAAGAATGTCCTTGCAGCTGAAGGATGTTGTCAGTTTTCTGACTGCAGCCAGCTTTCT
NM_001099270.1      TGGAAGAATGTCCTTGCAGCTGAAGGATGTTGTCAGTTTTCTGACTGCAGCCAGCTTTCT
                    ************************************************************

HsCD00295538        TCAGATGCAGTGTGTCATTGACAAGTGCACGCAGATCCTAGAGAGCATCCATTCCAAAAT
NM_001099270.1      TCAGATGCAGTGTGTCATTGACAAGTGCACGCAGATCCTAGAGAGCATCCATTCCAAAAT
                    ************************************************************

HsCD00295538        CAGCGTTGGAGATGTTGACTCTGTTACCGTCGGTGCTGAAGAGAATCCCGAGAGTCGAAA
NM_001099270.1      CAGCGTTGGAGATGTTGACTCTGTTACCGTCGGTGCTGAAGAGAATCCCGAGAGTCGAAA
                    ************************************************************

HsCD00295538        CGGAGTGAAAGACAGCAGCTTCTTTGCCAACCCAGTGGAGATCTCTCCTCCATATTGCTC
NM_001099270.1      CGGAGTGAAAGACAGCAGCTTCTTTGCCAACCCAGTGGAGATCTCTCCTCCATATTGCTC
                    ************************************************************

HsCD00295538        TCAGGGACGGCAGCCCACCGCAAGCAGTGACCTCCGGATGGAGACGACCCCCAGCAAAGC
NM_001099270.1      TCAGGGACGGCAGCCCACCGCAAGCAGTGACCTCCGGATGGAGACGACCCCCAGCAAAGC
                    ************************************************************

HsCD00295538        TTTGCGCAGCCGCTTACAGGAGGAGGGGCACTCAGACCGCGGGAGCAGTGGGAGCGTTTC
NM_001099270.1      TTTGCGCAGCCGCTTACAGGAGGAGGGGCACTCAGACCGCGGGAGCAGTGGGAGCGTTTC
                    ************************************************************

HsCD00295538        TGAATATGAGATTCAGATAGAGGGAGACCATGAGCAAGGAGACCTATTGGTGAGGGAGAG
NM_001099270.1      TGAATATGAGATTCAGATAGAGGGAGACCATGAGCAAGGAGACCTATTGGTGAGGGAGAG
                    ************************************************************

HsCD00295538        CCAGATCACCGAGGTGAAAGTGAAGATGGAGAAGTCCGACCGGCCCAGCTGTTCCGACAG
NM_001099270.1      CCAGATCACCGAGGTGAAAGTGAAGATGGAGAAGTCCGACCGGCCCAGCTGTTCCGACAG
                    ************************************************************

HsCD00295538        CTCCTCCCTGGGTGACGATGGGTACCACACCGAGATGGTTGATGGGGAACAAGTTGTGGC
NM_001099270.1      CTCCTCCCTGGGTGACGATGGGTACCACACCGAGATGGTTGATGGGGAACAAGTTGTGGC
                    ************************************************************

HsCD00295538        AGTGAATGTGGGCTCCTATGGTTCTGTGCTCCAGCACGCATACTCCTATTCCCAAGCAGC
NM_001099270.1      AGTGAATGTGGGCTCCTATGGTTCTGTGCTCCAGCACGCATACTCCTATTCCCAAGCAGC
                    ************************************************************

HsCD00295538        CTCACAGCCAACCAATGTATCAGAAGCTTTTGGAAGTTTGAGTAATTCCAGCCCATCCAG
NM_001099270.1      CTCACAGCCAACCAATGTATCAGAAGCTTTTGGAAGTTTGAGTAATTCCAGCCCATCCAG
                    ************************************************************

HsCD00295538        GTCCATGCTGAGCTGTTTCCGAGGAGGGCGTGCCCGCCAGAAGCGGGCTTTGTCTGTCCA
NM_001099270.1      GTCCATGCTGAGCTGTTTCCGAGGAGGGCGTGCCCGCCAGAAGCGGGCTTTGTCTGTCCA
                    ************************************************************

HsCD00295538        CCTGCACAGTGACCTGCAGGGCCTGGTGCAGGGCTCTGACAGTGAAGCCATGATGAACAA
NM_001099270.1      CCTGCACAGTGACCTGCAGGGCCTGGTGCAGGGCTCTGACAGTGAAGCCATGATGAACAA
                    ************************************************************

HsCD00295538        CCCCGGGTATGAGAGCAGTCCCCGGGAGAGGAGTGCGAGAGGGCATTGGTACCCGTACAA
NM_001099270.1      CCCCGGGTATGAGAGCAGTCCCCGGGAGAGGAGTGCGAGAGGGCATTGGTACCCGTACAA
                    ************************************************************

HsCD00295538        TGAGAGGTTGATCTGTATTTACTGTGGAAAGTCCTTCAACCAGAAAGGAAGCCTTGATAG
NM_001099270.1      TGAGAGGTTGATCTGTATTTACTGTGGAAAGTCCTTCAACCAGAAAGGAAGCCTTGATAG
                    ************************************************************

HsCD00295538        GCACATGCGACTCCATATGGGAATCACCCCCTTTGTGTGCAAGTTCTGTGGGAAGAAGTA
NM_001099270.1      GCACATGCGACTCCATATGGGAATCACCCCCTTTGTGTGCAAGTTCTGTGGGAAGAAGTA
                    ************************************************************

HsCD00295538        CACACGGAAGGACCAACTGGAGTACCACATCCGGGGCCATACAGATGATAAACCATTCCG
NM_001099270.1      CACACGGAAGGACCAACTGGAGTACCACATCCGGGGCCATACAGATGATAAACCATTCCG
                    ************************************************************

HsCD00295538        CTGTGAGATCTGCGGGAAGTGCTTTCCATTCCAAGGTACCCTCAACCAGCACTTGCGGAA
NM_001099270.1      CTGTGAGATCTGCGGGAAGTGCTTTCCATTCCAAGGTACCCTCAACCAGCACTTGCGGAA
                    ************************************************************

HsCD00295538        AAACCACCCAGGCGTTGCTGAAGTCAGGAGTCGCATTGAGTCCCCCGAGAGAACAGATGT
NM_001099270.1      AAACCACCCAGGCGTTGCTGAAGTCAGGAGTCGCATTGAGTCCCCCGAGAGAACAGATGT
                    ************************************************************

HsCD00295538        GTACGTGGAACAGAAACTAGAAAATGACGCATCGGCCTCAGAGATGGGCCTAGATTCCCG
NM_001099270.1      GTACGTGGAACAGAAACTAGAAAATGACGCATCGGCCTCAGAGATGGGCCTAGATTCCCG
                    ************************************************************

HsCD00295538        GATGGAAATTCACACAGTGTCTGATGCTCCCGATTAGGGCCTCATGGGCCCAGCTTTCTT
NM_001099270.1      GATGGAAATTCACACAGTGTCTGATGCTCCCGATTAA-----------------------
                    ************************************.                       

HsCD00295538        GTAC
NM_001099270.1      ----