Gene:
Gene Symbol:
ARHGEF4
Gene Name:
Rho guanine nucleotide exchange factor (GEF) 4
Original Clone ID:
FLH481892.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295550
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCCCTGGGAAGAACCAGCAGGTGA
GAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTCCACATGGAGCCTGCCCAGAAGCCCTG
CTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGACTGTGCGTGG
GGAGGCTCCTTCACAGCCTAGGGGCATCCCTCACCGCTCGCCCGTCAGTGTGGATGACCT
GTGGCTGGAGAAGACACAGAGAAAGAAGTTGCAGAAGCAGGCCCACGTCGAAAGGAGGCT
GCACATAGGGGCAGTGCACAAAGATGGAGTCAAGTGCTGGAGAAAGACGATCATTACCTC
TCCAGAGTCTTTGAATCTCCCTAGAAGAAGCCATCCACTCTCCCAGAGTGCTCCAACGGG
ACTGAACCACATGGGCTGGCCAGAGCACACACCAGGCACTGCCATGCCTGATGGAGCTCT
GGACACAGCTGTCTGCGCTGACGAAGTGGGGAGCGAGGAGGACCTGTATGATGACCTGCA
CAGCTCCAGCCACCACTACAGCCACCCTGGAGGGGGTGGGGAGCAGCTGGCTATCAATGA
GCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAAGCACTCTGGGACCATGTCACCATGGA
CGACCAGGAGCTGGGCTTCAAAGCTGGGGACGTCATCGAAGTGATGGATGCCACCAACAG
AGAGTGGTGGTGGGGCCGGGTCGCCGATGGCGAGGGCTGGTTTCCAGCCAGCTTCGTTCG
GCTGAGGGTGAATCAGGACGAGCCCGCGGATGACGACGCCCCTCTGGCCGGGAACAGCGG
AGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGCAAGGACCAGATGCGGACCAACGTCAT
CAACGAGATCCTCAGCACTGAGCGGGACTACATCAAGCACCTGCGCGACATCTGCGAGGG
CTACGTCCGGCAGTGCCGCAAGCGCGCAGACATGTTCAGCGAGGAGCAGCTGCGTACCAT
CTTCGGGAACATCGAGGACATCTACCGCTGCCAGAAGGCCTTCGTGAAGGCCCTGGAGCA
GAGGTTCAACCGCGAGCGCCCACACCTGAGCGAGCTGGGTGCCTGCTTCCTGGAGCATCA
AGCCGACTTCCAGATCTACTCGGAGTACTGCAATAACCACCCCAACGCCTGCGTGGAGCT
CTCCCGGCTCACCAAGCTCAGCAAGTACGTGTACTTCTTCGAGGCCTGCCGGCTGCTGCA
GAAGATGATTGACATCTCCCTGGATGGCTTCCTGCTGACTCCGGTGCAGAAGATCTGCAA
GTACCCTCTGCAGCTGGCCGAGCTGCTCAAATACACGCACCCCCAGCACAGGGACTTCAA
GGATGTTGAAGCCGCCTTGCATGCCATGAAGAACGTGGCCCAGCTCATCAACGAGCGGAA
GCGGAGACTTGAGAACATCGACAAGATTGCTCAGTGGCAGAGCTCCATAGAGGACTGGGA
GGGAGAAGATCTCTTGGTCAGGAGCTCAGAACTCATCTACTCGGGGGAGCTGACTCGAGT
TACACAGCCTCAAGCCAAAAGCCAGCAGCGAATGTTCTTTCTCTTTGACCACCAGCTCAT
CTACTGTAAGAAGGACCTGCTCCGCCGCGACGTGTTGTACTACAAGGGCCGGCTGGACAT
GGACGGCCTGGAGGTGGTGGACCTGGAGGACGGGAAGGACAGAGACCTCCATGTGAGCAT
CAAGAACGCCTTCCGGCTGCACCGTGGCGCCACAGGGGACAGCCACCTGCTGTGCACCAG
GAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCCTTTGCCAGGGAGAGGGAGCAGGTGCA
GCTGGACCAGGAGACAGGCTTCTCCATCACTGAACTGCAGAGGAAGCAGGCCATGCTGAA
TGCCAGCAAGCAGCAGGTCACAGGGAAGCCCAAAGCTGTTGGCCGGCCCTGCTACCTGAC
GCGCCAGAAGCACCCAGCCCTGCCCAGCAACCGGCCCCAGCAGCAGGTCCTGGTGCTGGC
GGAGCCCAGGCGCAAGCCATCTACCTTCTGGCACAGCATCAGCCGGCTGGCACCCTTCCG
CAAGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPH RSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSH PLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGG GGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGE GWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYI KHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSE LGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQ WQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDV LYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKA FAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNR PQQQVLVLAEPRRKPSTFWHSISRLAPFRK*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2593
Start on reference sequence
521
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
