Gene:
Gene Symbol:
SHANK2
Gene Name:
SH3 and multiple ankyrin repeat domains 2
Original Clone ID:
FLH481907.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295565
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGATGATGAACGTCCCCGGCGGAGG
AGCGGCCGCGGTGATGATGACGGGCTACAATAATGGTCGCTGTCCCCGGAATTCTCTCTA
CAGTGACTGCATTATTGAGGAGAAGACGGTGGTCCTGCAGAAAAAAGACAATGAGGGCTT
TGGATTCGTGCTTCGAGGGGCCAAAGCTGACACACCCATTGAAGAATTCACACCAACACC
GGCTTTCCCAGCCCTACAGTACCTGGAGTCCGTGGATGAAGGTGGGGTGGCGTGGCAAGC
CGGACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAATGAGAATGTTGTCAAAGTCGG
CCACAGGCAGGTGGTGAACATGATCCGGCAGGGAGGGAATCACCTGGTCCTTAAGGTGGT
CACGGTGACCAGGAATCTGGACCCCGACGACACCGCCAGGAAGAAAGCTCCCCCGCCTCC
AAAGCGGGCACCGACCACAGCCCTCACCCTGCGCTCCAAGTCCATGACCTCGGAGCTGGA
GGAGCTCGTGGATAAAGATAAACCCGAGGAGATAGTCCCGGCCTCCAAGCCCTCCCGCGC
TGCTGAGAACATGGCTGTGGAACCGAGGGTGGCGACCATCAAGCAGCGGCCCAGCAGCCG
GTGCTTCCCGGCGGGCTCAGACATGAACTCTGTGTACGAACGCCAAGGAATCGCCGTGAT
GACGCCCACTGTTCCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCTCGAGGTACGAT
GCGAAGGCAGAAATCAATAGACAGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGCG
GCAGTTTCTGGCTCCTCCAATGCTGAAGTTCACCAGAAGCCTGTCCATGCCGGACACCTC
TGAGGACATCCCCCCTCCACCGCAGTCTGTGCCCCCGTCCCCACCACCACCTTCCCCAAC
CACTTACAACTGCCCCAAGTCCCCAACTCCAAGAGTCTACGGGACGATTAAGCCTGCGTT
CAATCAGAATTCTGCCGCCAAGGTGTCCCCCGCCACCAGGTCCGACACCGTGGCCACCAT
GATGAGGGAGAAGGGGATGTACTTCAGGAGAGAGCTGGACCGCTACTCCTTGGACTCTGA
AGACCTCTACAGTCGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAGAGAGGCCAGAT
GCCAGAAAACCCATACTCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCGC
CAAGCCCGCCAGGCGGAAGGGGATGCTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGA
GAAGACGTGCTCCATCCCTATCCCGACCATCATCGTGAAGGAGCCGTCCACCAGCAGCAG
CGGCAAGAGCAGCCAGGGCAGCAGCATGGAGATCGACCCCCAGGCCCCGGAGCCACCGAG
CCAGCTGCGGCCTGACGAAAGCCTGACCGTCAGCAGCCCCTTTGCCGCCGCCATCGCCGG
AGCCGTCCGCGACCGTGAGAAGCGGCTGGAAGCCAGGAGGAACTCCCCGGCCTTCCTCTC
CACAGACCTGGGGGATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGGACGCGGCCCTC
CATGTTCCCCGAGGAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCCC
CATGCCGAGTGCCACGCCCAGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAG
TGCTCCGGGTGAGGCTGGGAGGCCGCTGAATTCCACGTCCAAAGCCCAGGGGCCCGAGAG
CAGCCCAGCAGTGCCCTCCGCGAGCAGCGGCACAGCCGGCCCCGGGAATTATGTCCACCC
ACTCACAGGGCGGCTGCTTGATCCCAGCTCCCCGCTGGCCCTGGCACTCTCCGCAAGGGA
CCGAGCCATGAAGGAGTCTCAACAGGGACCCAAAGGGGAGGCCCCCAAGGCCGACCTCAA
CAAACCTCTTTACATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGCTTCCCTACGGT
CACCAGGCAGAACACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGAC
CGACCTGGGCCGAGACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGA
CACGTCCCAGCAGAAGTCGGCTGGCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCT
GGACAACGCCCTGCAGGAAGAGGACGAGAAGGCAGAGGTGGAGATGAAGCCAGACAGCTC
GCCGTCCGAGGTGCCAGAAGGTGTTTCCGAAACCGAAGGTGCTTTACAGATCTCCGCTGC
CCCCGAGCCCACCACCGTGCCCGGCAGAACCATCGTCGCGGTGGGCTCCATGGAAGAGGC
GGTGATTTTGCCATTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGACTTGGATGAGGA
TTTTATTTTTACAGAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCCC
CGATGACCGGGCAGCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGA
CACCCCTCAGTCCCCTTCGTTGAACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAA
GCCAGCCAGTCTTTCAAACTGTCTGCCTGCCTCATTCCTGCCACCCCCTGAAAGCTTTGA
CGCCGTCGCCGACTCTGGGATCGAGGAGGTGGACAGCCGGAGTAGCAGCGACCACCACCT
CGAGACGACCAGCACTATCTCCACCGTGTCTAGCATCTCCACCCTGTCTTCCGAAGGTGG
AGAGAATGTGGACACCTGCACAGTCTATGCAGATGGGCAAGCATTTATGGTTGACAAACC
CCCAGTACCTCCTAAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATCA
AGACGCGCTCGTGGAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCC
CCCGCCGGGCAGTGCCCAGCCTGGGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTT
GTGGGGCGACGTCACAGAGATCAAAAGCCCGATTCTCTCAGGCCCAAAGGCAAACGTTAT
TAGTGAATTGAACTCTATCCTACAGCAAATGAACCGAGAGAAATTGGCAAAGCCGGGGGA
AGGACTGGATTCACCAATGGGAGCCAAGTCCGCCAGCCTCGCTCCAAGAAGCCCGGAGAT
CATGAGCACCATCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTTCGCCCCGGCAC
CTCCCAGCCCATCACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCAGGAAC
AAGACGTGCCCCAAGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGC
CCCCCTGTCTGCTGCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCC
AAGCCAGCCCCCTTCTGGGGATCTATTTGGCTTGAACCCAGCGGGACGCAGTAGGTCGCC
ATCCCCCTCGATACTGCAACAGCCAATCTCAAATAAGCCTTTTACAACTAAACCTGTCCA
CCTGTGGACTAAACCAGATGTGGCCGATTGGCTGGAAAGTCTAAACTTGGGTGAACATAA
AGAGGCCTTCATGGACAATGAGATCGATGGCAGTCACTTACCAAACCTGCAGAAGGAGGA
CCTCATCGATCTTGGGGTAACTCGAGTCGGGCACAGAATGAACATAGAAAGGGCTTTGAA
ACAGCTGCTGGACAGATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MMMNVPGGGAAAVMMTGYNNGRCPRNSLYSDCIIEEKTVVLQKKDNEGFGFVLRGAKADT PIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQG GNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTSELEELVDKDKPEEI VPASKPSRAAENMAVEPRVATIKQRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAP FLGIPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVP PSPPPPSPTTYNCPKSPTPRVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRE LDRYSLDSEDLYSRNAGPQANFRNKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVK QSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQAPEPPSQLRPDESLTVS SPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADE DSAEQLSSPMPSATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGT AGPGNYVHPLTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRP SLDAGFPTVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLM VHTVDATKLDNALQEEDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTI VAVGSMEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALS DLVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVD SRSSSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPI IHKSNALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPI LSGPKANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTT VTFTVRPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSP ALSDVFSLPSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWL ESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3765
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
