Gene:
Gene Symbol:
SKOR1
Gene Name:
SKI family transcriptional corepressor 1
Original Clone ID:
FLH481911.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295569
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2763nts
Open reading frame: 1 to 2763
ATGGCTTTGCTGTGTGGCCTTGGGCAAGTCACTCTGCGTATCTGGGTTTCACTTCCTTCC
CAATCCGAAAACGGGATTGGGAGCGGCGGCATGGAGGCTCTCACCACTCAGCTGGGGCCG
GGGCGCGAGGGCAGTTCCTCGCCCAACTCCAAGCAGGAGCTGCAGCCGTACTCGGGCTCC
AGCGCTCTCAAACCCAACCAGGTGGGCGAGACGTCGCTGTACGGGGTGCCCATTGTGTCG
CTGGTCATCGACGGCCAGGAGCGCCTATGCCTGGCGCAGATCTCCAACACCCTCCTCAAG
AACTACAGCTATAATGAGATCCACAACCGCCGCGTGGCCCTGGGCATCACGTGCGTGCAG
TGCACGCCGGTACAGCTGGAGATTCTGCGTCGGGCCGGGGCCATGCCCATCTCGTCGCGC
CGCTGCGGCATGATCACTAAGCGAGAGGCCGAACGCCTGTGCAAGTCGTTCCTGGGCGAG
CACAAACCACCCAAGCTGCCCGAGAACTTCGCCTTCGATGTGGTGCACGAGTGCGCGTGG
GGCTCGCGTGGTAGCTTCATCCCTGCGCGTTACAACAGCTCTCGTGCCAAGTGCATCAAG
TGCGGCTACTGCAGCATGTACTTCTCGCCCAACAAGTTCATCTTCCACTCGCACCGAACA
CCCGACGCCAAGTACACGCAGCCCGATGCCGCCAACTTCAACTCCTGGCGTCGTCACCTC
AAACTCAGTGACAAGTCGGCCACAGACGAACTGAGCCATGCTTGGGAGGACGTCAAGGCC
ATGTTTAATGGCGGCACGCGGTGCGGTGCAGAGATGGCCCCAGGCCCGCCGCCCCACAAA
AGCCTGCGCTGTGGCGAAGATGAGGCTGCCGGGCCTCCGGGGCCACCTCCACCCCACCCG
CAGCGCGGACTTGGCCTGGCGACTGGAGCTAGTGGCCCGGCGGGCCCAGGAGGGCCCGGT
GGCGGCGCCGGCGTACGAAGCTACCCGGTGATCCCGGTGCCCAGCAAAGGCTTTGGGCTC
CTGCAAAAGCTGCCCCCACCACTTTTCCCCCATCCTTACGGCTTCCCTACGGCCTTCGGC
CTATGCCCCAAAAAGGACGACCCGGTTTTAGGCGCGGGCGAGCCAAAGGGCGGTCCTGGC
ACTGGGAGCGGCGGCGGCGGCGCGGGGACAGGCGGGGGTGCGGGGGGCCCGGGAGCCAGC
CACTTGCCCCCGGGGGCAGGGGCGGGCCCGGGCGGCGGCGCCATGTTCTGGGGGCATCAA
CCCTCCGGGGCAGCCAAGGACGCAGCGGCAGTGGCTGCAGCGGCCGCCGCCGCCACTGTG
TACCCGACGTTTCCCATGTTCTGGCCAGCAGCAGGCAGCCTCCCGGTACCGTCCTACCCC
GCTGCTCAGAGCCAAGCCAAGGCCGTGGCGGCAGCCGTGGCGGCGGCAGCGGCGGCGGCA
GCGGCAGCTGCTGGCAGCGGTGCCCCAGAGCCCCTGGACGGTGCCGAGCCAGCCAAAGAG
AGTGGCCTCGGCGCGGAGGAGCGCTGCCCGAGCGCTCTGTCCCGCGGGCCCCTGGACGAA
GACGGCACGGACGAGGCGCTGCCACCGCCCCTGGCCCCGTTGCCCCCGCCGCCCCCGCCG
CCCGCACGCAAAGGCTCCTACGTGTCGGCCTTCCGGCCGGTGGTCAAGGACACCGAGAGC
ATCGCTAAGCTCTACGGGAGCGCCCGGGAGGCGTACGGCGCGGGGCCTGCTCGGGGGCCG
GGACCCGGCGCTGGGAGCGGCGGCTACGTGAGCCCGGACTTTCTGAGCGAGGGCAGCTCC
AGCTACAATTCCGCCTCGCCCGACGTGGACACCGCGGACGAGCCCGAGGTGGACGTGGAA
TCCAACCGCTTCCCCGACGACGAGGACGCCCAAGAGGAGACCGAGCCCAGCGCACCCAGC
GCAGGGGGCGGCCCAGACGGTGAACAGCCCACTGGACCCCCTTCCGCCACCTCCTCTGGC
GCGGACGGTCCCGCAAACTCTCCCGACGGCGGCAGCCCCCGCCCCCGGCGCCGCCTCGGG
CCACCCCCAGCTGGCCGGCCCGCATTTGGGGACTTGGCAGCCGAAGACTTGGTGCGGAGA
CCTGAGAGGAGCCCGCCAAGCGGCGGCGGCGGCTACGAGCTGCGAGAGCCTTGCGGGCCC
CTAGGAGGCCCCGCGCCGGCCAAGGTGTTCGCGCCCGAGAGGGATGAGCACGTGAAGAGC
GCGGCGGTGGCGCTGGGGCCCGCGGCCTCCTACGTCTGCACCCCCGAGGCCCACGAGCCA
GATAAGGAAGACAATCACTCGCCCGCCGATGATTTGGAAACGAGGAAATCCTATCCAGAC
CAAAGGAGTATCTCCCAGCCAAGTCCTGCAAATACAGACAGAGGCGAAGATGGGCTTACC
TTGGATGTCACAGGAACTCATTTGGTGGAGAAAGATATCGAGAACCTGGCCAGAGAGGAA
TTGCAAAAACTGCTCCTGGAACAAATGGAGCTCCGCAAGAAGCTGGAACGGGAATTTCAG
AGTCTCAAAGATAATTTTCAGGATCAAATGAAGAGGGAATTGGCTTATCGAGAAGAAATG
GTGCAACAGCTGCAAATTGTCAGAGATACCCTGTGTAACGAACTCGACCAGGAGCGGAAG
GCGCGCTATGCCATCCAGCAGAAATTGAAAGAAGCCCACGACGCCCTGCACCATTTCTCC
TGCAAGATGCTGACGCCCCGCCACTGCACTGGCAACTGCTCCTTCAAGCCACCGCTGTTG
CCC
Protein sequence predicted based on nucleotide sequence
MALLCGLGQVTLRIWVSLPSQSENGIGSGGMEALTTQLGPGREGSSSPNSKQELQPYSGS SALKPNQVGETSLYGVPIVSLVIDGQERLCLAQISNTLLKNYSYNEIHNRRVALGITCVQ CTPVQLEILRRAGAMPISSRRCGMITKREAERLCKSFLGEHKPPKLPENFAFDVVHECAW GSRGSFIPARYNSSRAKCIKCGYCSMYFSPNKFIFHSHRTPDAKYTQPDAANFNSWRRHL KLSDKSATDELSHAWEDVKAMFNGGTRCGAEMAPGPPPHKSLRCGEDEAAGPPGPPPPHP QRGLGLATGASGPAGPGGPGGGAGVRSYPVIPVPSKGFGLLQKLPPPLFPHPYGFPTAFG LCPKKDDPVLGAGEPKGGPGTGSGGGGAGTGGGAGGPGASHLPPGAGAGPGGGAMFWGHQ PSGAAKDAAAVAAAAAAATVYPTFPMFWPAAGSLPVPSYPAAQSQAKAVAAAVAAAAAAA AAAAGSGAPEPLDGAEPAKESGLGAEERCPSALSRGPLDEDGTDEALPPPLAPLPPPPPP PARKGSYVSAFRPVVKDTESIAKLYGSAREAYGAGPARGPGPGAGSGGYVSPDFLSEGSS SYNSASPDVDTADEPEVDVESNRFPDDEDAQEETEPSAPSAGGGPDGEQPTGPPSATSSG ADGPANSPDGGSPRPRRRLGPPPAGRPAFGDLAAEDLVRRPERSPPSGGGGYELREPCGP LGGPAPAKVFAPERDEHVKSAAVALGPAASYVCTPEAHEPDKEDNHSPADDLETRKSYPD QRSISQPSPANTDRGEDGLTLDVTGTHLVEKDIENLAREELQKLLLEQMELRKKLEREFQ SLKDNFQDQMKRELAYREEMVQQLQIVRDTLCNELDQERKARYAIQQKLKEAHDALHHFS CKMLTPRHCTGNCSFKPPLLP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2766
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
