Gene:
Gene Symbol:
LPHN1
Gene Name:
latrophilin 1
Original Clone ID:
FLH481913.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295571
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCCGCCTAGCCGCAGTGCTCTGGAATCTGTGTGTCACCGCCGTCCTGGTCACCTCG
GCCACCCAAGGCCTGAGCCGGGCCGGGCTCCCGTTCGGGCTGATGCGCCGGGAGCTGGCG
TGTGAAGGCTACCCCATCGAGCTGCGGTGCCCCGGCAGCGACGTCATCATGGTGGAGAAT
GCCAACTACGGGCGCACGGACGACAAGATTTGCGATGCTGACCCTTTCCAGATGGAGAAT
GTGCAGTGCTACCTGCCGGACGCCTTCAAGATCATGTCACAGAGGTGTAACAACCGCACC
CAGTGCGTGGTGGTCGCCGGCTCGGATGCCTTTCCTGACCCCTGTCCTGGGACCTACAAG
TACCTGGAGGTGCAGTACGACTGTGTCCCCTACATCTTCGTGTGCCCAGGGACCCTGCAG
AAGGTGCTGGAGCCCACCTCGACACACGAGTCAGAGCACCAGTCTGGCGCATGGTGCAAG
GACCCGCTGCAGGCGGGTGACCGCATCTACGTGATGCCCTGGATCCCCTACCGCACGGAC
ACACTGACTGAGTATGCCTCGTGGGAGGACTACGTGGCCGCCCGCCACACCACCACCTAC
CGCCTGCCCAACCGCGTGGATGGCACAGGCTTTGTGGTCTACGATGGTGCCGTCTTCTAC
AACAAGGAGCGCACGCGCAACATCGTCAAGTATGACCTACGGACGCGCATCAAGAGCGGG
GAGACGGTCATCAATACCGCCAACTACCATGACACCTCGCCCTACCGCTGGGGCGGAAAG
ACCGACATTGACCTGGCGGTGGACGAGAACGGGCTGTGGGTCATCTACGCCACTGAGGGC
AACAACGGGCGGCTGGTGGTGAGCCAGCTGAACCCCTACACACTGCGCTTTGAGGGCACG
TGGGAGACGGGTTACGACAAGCGCTCGGCATCCAACGCCTTCATGGTGTGTGGGGTCCTG
TACGTCCTGCGTTCCGTGTACGTGGATGATGACAGCGAGGCGGCTGGCAACCGCGTGGAC
TATGCCTTCAACACCAATGCCAACCGCGAGGAGCCTGTCAGCCTCACCTTCCCCAACCCC
TACCAGTTCATCTCCTCCGTTGACTACAACCCTCGCGACAACCAGCTGTACGTCTGGAAC
AACTATTTCGTGGTGCGCTACAGCCTGGAGTTCGGGCCGCCCGACCCCAGTGCTGGCCCA
GCCACTTCCCCACCCCTCAGCACGACCACCACAGCCAGGCCCACGCCCCTCACCAGCACA
GCCTCGCCCGCAGCCACCACCCCGCTCCGCCGGGCACCCCTCACCACGCACCCAGTGGGT
GCCATCAACCAGCTGGGACCTGATCTGCCTCCAGCCACAGCCCCAGTCCCCAGCACCCGG
CGGCCCCCAGCCCCGAATCTACACGTGTCCCCTGAGCTCTTCTGCGAGCCCCGAGAGGTA
CGGCGGGTCCAGTGGCCGGCCACCCAGCAGGGCATGCTGGTGGAGAGGCCCTGCCCCAAG
GGGACTCGAGGAATTGCCTCCTTCCAGTGTCTACCAGCCTTGGGGCTCTGGAACCCCCGG
GGCCCTGACCTCAGCAACTGCACCTCCCCCTGGGTCAACCAGGTGGCCCAGAAGATCAAG
AGTGGGGAGAACGCGGCCAACATCGCCAGCGAGCTGGCCCGACACACCCGGGGCTCCATC
TACGCGGGGGACGTCTCCTCCTCTGTGAAGCTGATGGAGCAGCTGCTGGACATCCTGGAT
GCCCAGCTGCAGGCCCTGCGGCCCATCGAGCGCGAGTCAGCCGGCAAGAACTACAACAAG
ATGCACAAGCGAGAGAGAACTTGTAAGGATTATATCAAGGCCGTGGTGGAGACAGTGGAC
AATCTGCTCCGGCCAGAAGCTCTGGAGTCCTGGAAGGACATGAATGCCACGGAGCAGGTG
CACACGGCCACCATGCTCCTCGACGTCCTGGAGGAGGGCGCCTTCCTGCTGGCCGACAAT
GTCAGGGAGCCTGCCCGCTTCCTGGCTGCCAAGGAGAACGTGGTCCTGGAGGTCACAGTC
CTGAACACAGAGGGCCAGGTGCAGGAGCTGGTGTTCCCCCAGGAGGAGTACCCGAGAAAG
AACTCCATCCAGCTGTCTGCCAAAACCATCAAGCAGAACAGCCGCAATGGGGTGGTCAAA
GTTGTCTTCATCCTCTACAACAACCTGGGCCTCTTCCTGTCCACGGAGAATGCCACAGTG
AAGCTGGCCGGCGAAGCAGGCCCGGGTGGCCCTGGGGGCGCCTCTCTAGTGGTGAACTCA
CAGGTCATCGCAGCATCCATCAACAAGGAGTCCAGCCGCGTCTTCCTCATGGACCCTGTC
ATCTTCACCGTGGCCCACCTGGAGGACAAGAACCACTTCAATGCTAACTGCTCCTTCTGG
AACTACTCGGAGCGTTCCATGCTGGGCTACTGGTCGACCCAAGGCTGCCGCCTGGTGGAG
TCCAACAAGACCCATACCACGTGTGCCTGCAGCCACCTCACCAACTTCGCTGTGCTCATG
GCTCACCGTGAGATCTACCAGGGCCGCATCAACGAGCTGCTGCTGTCGGTCATCACCTGG
GTGGGCATTGTGATCTCCCTGGTCTGCTTGGCCATCTGCATCTCCACCTTCTGCTTCCTG
CGGGGGCTGCAGACCGACCGCAACACCATCCACAAGAACCTGTGCATCAACCTCTTCCTG
GCTGAGCTGCTCTTCCTGGTCGGGATCGACAAGACTCAGTATGAGATTGCCTGCCCCATC
TTCGCCGGCCTGCTGCACTATTTCTTCCTGGCTGCCTTCTCCTGGCTGTGCCTGGAGGGC
GTGCACCTCTACCTGCTACTAGTGGAGGTGTTTGAGAGCGAGTATTCCCGCACCAAGTAC
TACTACCTGGGTGGCTACTGCTTCCCGGCCCTGGTGGTGGGCATCGCGGCTGCCATTGAC
TACCGCAGCTACGGCACCGAGAAGGCCTGCTGGCTCCGAGTGGACAATTACTTCATCTGG
AGTTTCATCGGGCCAGTCTCCTTCGTTATCGTGGTCAACCTGGTGTTCCTCATGGTGACC
CTGCACAAGATGATCCGAAGCTCATCTGTGCTCAAGCCCGACTCCAGCCGCCTGGACAAC
ATTAAATCCTGGGCGCTGGGGGCCATCGCGCTGCTGTTCCTGCTGGGCCTCACCTGGGCT
TTCGGCCTCCTCTTCATCAACAAGGAGTCGGTGGTCATGGCCTATCTCTTCACCACCTTC
AACGCCTTCCAGGGGGTCTTCATCTTCGTCTTTCACTGCGCCTTACAGAAGAAGGTGCAC
AAGGAGTACAGCAAGTGCCTGCGTCACTCCTACTGCTGCATCCGCTCCCCACCCGGGGGC
ACTCACGGATCCCTCAAGACCTCAGCCATGCGAAGCAACACCCGCTACTACACAGGGACC
CAGAGCCGAATTCGGAGGATGTGGAATGACACTGTGAGGAAACAGACGGAGTCCTCCTTC
ATGGCGGGTGACATCAACAGCACCCCCACCCTGAACCGAGGTACCATGGGGAACCACCTG
CTGACCAACCCCGTGCTGCAGCCCCGTGGGGGCACCAGTCCCTACAACACCCTCATCGCC
GAGTCAGTGGGCTTCAATCCCTCCTCGCCCCCTGTCTTCAACTCCCCAGGGAGCTACCGG
GAACCCAAGCACCCCTTGGGAGGCCGGGAAGCCTGTGGCATGGACACCCTGCCCCTGAAC
GGCAACTTCAATAACAGTTACTCCTTGCGAAGTGGGGATTTCCCTCCCGGGGATGGGGGC
CCTGAGCCGCCCCGAGGCCGGAACCTAGCCGATGCGGCGGCCTTTGAGAAGATGATCATC
TCAGAGCTGGTGCACAACAACCTGCGGGGGAGCAGCAGCGCGGCCAAGGGCCCTCCACCG
CCTGAGCCCCCTGTGCCACCTGTGCCAGGGGGCGGGGGCGAGGAAGAGGCGGGCGGGCCC
GGGGGTGCTGACCGGGCCGAGATTGAACTTCTCTATAAGGCCCTGGAGGAGCCTCTGCTG
CTGCCCCGGGCCCAGTCGGTGCTGTACCAGAGCGATCTGGACGAGTCGGAGAGCTGCACG
GCCGAGGACGGCGCCACCAGCCGGCCCCTCTCCTCCCCTCCTGGCCGGGACTCCCTCTAT
GCCAGCGGGGCCAACCTGCGGGACTCACCCTCCTACCCGGACAGCAGCCCTGAGGGGCCC
AGTGAGGCCCTGCCCCCACCCCCTCCCGCACCCCCCGGCCCCCCCGAAATCTACTACACC
TCGCGCCCGCCAGCCCTGGTGGCCCGGAATCCCCTGCAGGGCTACTACCAGGTGCGGCGT
CCTAGCCACGAGGGCTACCTGGCAGCCCCAGGCCTTGAGGGGCCAGGGCCCGATGGGGAC
GGGCAGATGCAGCTGGTCACCAGTCTC
Protein sequence predicted based on nucleotide sequence
MARLAAVLWNLCVTAVLVTSATQGLSRAGLPFGLMRRELACEGYPIELRCPGSDVIMVEN ANYGRTDDKICDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVVVAGSDAFPDPCPGTYK YLEVQYDCVPYIFVCPGTLQKVLEPTSTHESEHQSGAWCKDPLQAGDRIYVMPWIPYRTD TLTEYASWEDYVAARHTTTYRLPNRVDGTGFVVYDGAVFYNKERTRNIVKYDLRTRIKSG ETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVIYATEGNNGRLVVSQLNPYTLRFEGT WETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVDYAFNTNANREEPVSLTFPNP YQFISSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAGPATSPPLSTTTTARPTPLTST ASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPSTRRPPAPNLHVSPELFCEPREV RRVQWPATQQGMLVERPCPKGTRGIASFQCLPALGLWNPRGPDLSNCTSPWVNQVAQKIK SGENAANIASELARHTRGSIYAGDVSSSVKLMEQLLDILDAQLQALRPIERESAGKNYNK MHKRERTCKDYIKAVVETVDNLLRPEALESWKDMNATEQVHTATMLLDVLEEGAFLLADN VREPARFLAAKENVVLEVTVLNTEGQVQELVFPQEEYPRKNSIQLSAKTIKQNSRNGVVK VVFILYNNLGLFLSTENATVKLAGEAGPGGPGGASLVVNSQVIAASINKESSRVFLMDPV IFTVAHLEDKNHFNANCSFWNYSERSMLGYWSTQGCRLVESNKTHTTCACSHLTNFAVLM AHREIYQGRINELLLSVITWVGIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFL AELLFLVGIDKTQYEIACPIFAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKY YYLGGYCFPALVVGIAAAIDYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVT LHKMIRSSSVLKPDSSRLDNIKSWALGAIALLFLLGLTWAFGLLFINKESVVMAYLFTTF NAFQGVFIFVFHCALQKKVHKEYSKCLRHSYCCIRSPPGGTHGSLKTSAMRSNTRYYTGT QSRIRRMWNDTVRKQTESSFMAGDINSTPTLNRGTMGNHLLTNPVLQPRGGTSPYNTLIA ESVGFNPSSPPVFNSPGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFPPGDGG PEPPRGRNLADAAAFEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEEEAGGP GGADRAEIELLYKALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLY ASGANLRDSPSYPDSSPEGPSEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRR PSHEGYLAAPGLEGPGPDGDGQMQLVTSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
297
End on reference sequence
4706
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
