Gene:
Gene Symbol:
ATRNL1
Gene Name:
attractin-like 1
Original Clone ID:
FLH481917.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295575
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGACTGGGGGCCGGGCCCGCACTGGTACCCCGCAGCCAGCGGCCCCGGGGGTGTGG
AGGGCTCGGCCGGCGGGCGGCGGCGGCGGGGGCGCCTCCTCCTGGCTGCTGGACGGGAAC
AGCTGGCTGCTGTGCTATGGCTTCCTCTACCTGGCGCTCTACGCGCAGGTGTCCCAGTCC
AAGCCGTGCGAGAGGACCGGCTCCTGCTTCTCGGGCCGCTGTGTCAACTCCACCTGCCTC
TGCGACCCGGGCTGGGTGGGGGACCAGTGCCAGCACTGCCAGGGCAGGTTCAAGTTAACA
GAACCTTCTGGATATTTAACAGATGGCCCAATTAACTATAAATATAAAACTAAATGTACT
TGGCTCATTGAAGGCTATCCAAATGCAGTGTTAAGATTAAGATTCAATCATTTTGCTACA
GAATGTAGCTGGGATCATATGTATGTTTATGATGGAGATTCAATATATGCACCTTTAATA
GCTGTACTTAGTGGTTTGATAGTCCCTGAAATAAGGGGCAATGAAACTGTGCCTGAAGTT
GTTACTACATCTGGCTATGCACTGTTACATTTTTTTAGTGATGCTGCGTATAATCTAACT
GGTTTCAACATTTTCTATTCAATCAATTCTTGTCCTAACAATTGCTCTGGTCATGGGAAG
TGTACAACTAGTGTCTCTGTTCCAAGTCAAGTATATTGTGAATGTGATAAATACTGGAAG
GGTGAAGCTTGTGATATTCCTTACTGTAAAGCCAATTGCGGCAGTCCAGATCACGGTTAC
TGTGACCTGACTGGAGAAAAATTATGTGTCTGCAATGATAGTTGGCAAGGTCCTGATTGT
TCTTTGAATGTTCCCTCTACTGAGTCTTACTGGATTCTGCCAAACGTTAAACCCTTCAGT
CCTTCTGTAGGTCGGGCTTCACATAAAGCAGTTTTACACGGGAAATTTATGTGGGTGATT
GGTGGATATACTTTTAACTACAGTTCTTTTCAAATGGTCCTAAATTACAATTTAGAAAGC
AGTATATGGAATGTAGGAACTCCATCAAGGGGACCTCTCCAGAGATATGGACACTCTCTT
GCTTTATATCAGGAAAACATCTTTATGTATGGAGGCAGAATTGAAACAAATGATGGCAAT
GTCACAGATGAATTATGGGTTTTTAACATACATAGTCAGTCATGGAGTACAAAAACTCCT
ACTGTTCTTGGACATGGTCAGCAGTATGCTGTGGAGGGACATTCAGCACATATTATGGAG
TTGGATAGTAGAGATGTTGTCATGATCATAATATTTGGATATTCTGCAATATATGGTTAT
ACAAGCAGCATACAGGAATACCATATCTCATCAAACACTTGGCTTGTTCCAGAAACTAAA
GGAGCTATTGTACAAGGTGGATATGGCCATACTAGTGTGTATGATGAAATAACAAAGTCC
ATTTATGTTCATGGAGGGTATAAAGCATTGCCAGGGAACAAATATGGATTGGTTGATGAT
CTTTATAAATATGAAGTTAACACTAAGACTTGGACTATTTTGAAAGAAAGTGGGTTTGCC
AGATACCTTCATTCAGCTGTTCTTATCAATGGAGCTATGCTTATTTTTGGAGGAAATACC
CATAATGACACTTCCTTGAGTAACGGTGCAAAATGTTTTTCTGCCGATTTCCTGGCATAT
GACATAGCTTGTGATGAATGGAAAATACTACCAAAACCAAATCTTCATAGAGATGTCAAC
AGATTTGGACACTCTGCAGTAGTCATTAACGGGTCCATGTATATATTTGGGGGATTTTCT
AGTGTACTCCTTAATGATATCCTTGTATACAAGCCTCCAAATTGCAAGGCTTTCAGAGAT
GAAGAACTTTGTAAAAATGCTGGTCCAGGGATAAAATGTGTTTGGAATAAAAATCACTGT
GAATCTTGGGAATCTGGGAATACTAATAATATTCTTAGAGCAAAGTGCCCTCCTAAAACA
GCTGCTTCTGATGACAGATGTTACAGATATGCAGATTGTGCCAGCTGTACTGCCAATACA
AATGGGTGCCAATGGTGTGATGACAAGAAATGCATTTCGGCAAATAGTAACTGCAGTATG
TCTGTCAAGAACTACACCAAATGTCATGTGAGAAATGAGCAGATTTGTAACAAACTTACC
AGCTGTAAAAGCTGTTCACTAAACTTGAATTGCCAGTGGGATCAGAGACAGCAAGAATGC
CAGGCTTTACCAGCTCATCTTTGTGGAGAAGGATGGAGTCATATTGGGGATGCTTGTCTT
AGAGTCAATTCCAGTAGAGAAAACTATGACAATGCAAAACTTTATTGCTATAATCTTAGT
GGAAATCTTGCTTCATTAACAACCTCAAAAGAAGTAGAATTTGTTCTGGATGAAATACAG
AAGTATACACAACAGAAAGTATCACCTTGGGTAGGCTTGCGCAAGATCAATATATCCTAT
TGGGGATGGGAAGACATGTCTCCTTTTACAAACACAACACTACAGTGGCTTCCTGGCGAA
CCCAATGATTCTGGGTTTTGTGCATATCTGGAAAGGGCTGCAGTGGCAGGCTTAAAAGCT
AATCCTTGTACATCTATGGCAAATGGCCTTGTCTGTGAAAAACCTGTTGTTAGTCCAAAT
CAAAATGCGAGGCCGTGCAAAAAGCCATGCTCTCTGAGGACATCATGTTCCAACTGTACA
AGCAATGGCATGGAGTGTATGTGGTGCAGCAGTACGAAACGATGTGTTGACTCTAATGCC
TATATCATCTCTTTTCCATATGGACAATGTCTAGAGTGGCAAACTGCCACCTGCTCCCCT
CAAAATTGTTCTGGATTGAGAACCTGTGGACAGTGTTTGGAACAGCCTGGATGTGGCTGG
TGCAATGATCCTAGTAATACAGGAAGAGGACATTGCATTGAAGGTTCTTCACGGGGACCA
ATGAAGCTTATTGGAATGCACCACAGTGAGATGGTTCTTGACACCAATCTTTGCCCCAAA
GAAAAGAACTATGAGTGGTCCTTTATCCAGTGTCCAGCTTGCCAGTGTAATGGACATAGC
ACTTGCATCAATAATAATGTGTGCGAACAGTGTAAAAATCTCACCACAGGAAAGCAGTGT
CAAGATTGTATGCCAGGTTATTATGGAGATCCAACCAATGGTGGACAGTGCACAGCTTGT
ACATGCAGTGGCCATGCAAATATCTGTCATCTGCACACAGGAAAATGTTTCTGCACAACT
AAAGGAATAAAAGGTGACCAATGCCAATTATGTGACTCTGAAAATCGCTATGTTGGTAAT
CCACTTAGAGGAACATGTTATTACAGCCTTTTGATTGATTATCAATTTACCTTCAGCTTA
TTACAGGAAGATGATCGCCACCATACTGCCATAAACTTTATAGCAAACCCAGAACAGTCG
AACAAAAATCTGGATATATCAATTAATGCATCAAACAACTTTAATCTCAACATTACGTGG
TCTGTCGGTTCAACAGCTGGAACAATATCTGGGGAAGAGACTTCTATAGTTTCCAAGAAT
AATATAAAGGAATACAGAGATAGTTTTTCCTATGAAAAATTTAACTTTAGAAGCAATCCT
AACATTACATTCTATGTGTACGTCAGCAACTTTTCCTGGCCTATTAAAATACAGATTGCA
TTCTCACAACACAATACAATCATGGACCTTGTGCAGTTTTTTGTCACCTTCTTCAGTTGT
TTCCTATCCTTATTGCTGGTGGCTGCTGTGGTATGGAAGATCAAACAAACTTGTTGGGCT
TCTCGACGGAGAGAGCAACTGCTTCGAGAACGACAGCAGATGGCCAGCCGTCCCTTTGCT
TCTGTTGATGTAGCTCTGGAAGTGGGAGCTGAACAAACAGAGTTTCTGCGAGGGCCATTA
GAGGGGGCACCCAAGCCAATTGCCATTGAACCATGTGCTGGGAACAGAGCTGCTGTTCTG
ACTGTGTTTCTTTGTCTACCACGAGGATCATCAGGTGCCCCTCCCCCTGGGCAGTCAGGC
CTTGCAATTGCCAGTGCCCTAATAGATATTTCACAACAGAAAGCTTCAGATAGTAAAGAT
AAGACTTCTGGAGTCCGGAATCGAAAACACCTTTCAACACGTCAAGGAACTTGTGTC
Protein sequence predicted based on nucleotide sequence
METGGRARTGTPQPAAPGVWRARPAGGGGGGASSWLLDGNSWLLCYGFLYLALYAQVSQS KPCERTGSCFSGRCVNSTCLCDPGWVGDQCQHCQGRFKLTEPSGYLTDGPINYKYKTKCT WLIEGYPNAVLRLRFNHFATECSWDHMYVYDGDSIYAPLIAVLSGLIVPEIRGNETVPEV VTTSGYALLHFFSDAAYNLTGFNIFYSINSCPNNCSGHGKCTTSVSVPSQVYCECDKYWK GEACDIPYCKANCGSPDHGYCDLTGEKLCVCNDSWQGPDCSLNVPSTESYWILPNVKPFS PSVGRASHKAVLHGKFMWVIGGYTFNYSSFQMVLNYNLESSIWNVGTPSRGPLQRYGHSL ALYQENIFMYGGRIETNDGNVTDELWVFNIHSQSWSTKTPTVLGHGQQYAVEGHSAHIME LDSRDVVMIIIFGYSAIYGYTSSIQEYHISSNTWLVPETKGAIVQGGYGHTSVYDEITKS IYVHGGYKALPGNKYGLVDDLYKYEVNTKTWTILKESGFARYLHSAVLINGAMLIFGGNT HNDTSLSNGAKCFSADFLAYDIACDEWKILPKPNLHRDVNRFGHSAVVINGSMYIFGGFS SVLLNDILVYKPPNCKAFRDEELCKNAGPGIKCVWNKNHCESWESGNTNNILRAKCPPKT AASDDRCYRYADCASCTANTNGCQWCDDKKCISANSNCSMSVKNYTKCHVRNEQICNKLT SCKSCSLNLNCQWDQRQQECQALPAHLCGEGWSHIGDACLRVNSSRENYDNAKLYCYNLS GNLASLTTSKEVEFVLDEIQKYTQQKVSPWVGLRKINISYWGWEDMSPFTNTTLQWLPGE PNDSGFCAYLERAAVAGLKANPCTSMANGLVCEKPVVSPNQNARPCKKPCSLRTSCSNCT SNGMECMWCSSTKRCVDSNAYIISFPYGQCLEWQTATCSPQNCSGLRTCGQCLEQPGCGW CNDPSNTGRGHCIEGSSRGPMKLIGMHHSEMVLDTNLCPKEKNYEWSFIQCPACQCNGHS TCINNNVCEQCKNLTTGKQCQDCMPGYYGDPTNGGQCTACTCSGHANICHLHTGKCFCTT KGIKGDQCQLCDSENRYVGNPLRGTCYYSLLIDYQFTFSLLQEDDRHHTAINFIANPEQS NKNLDISINASNNFNLNITWSVGSTAGTISGEETSIVSKNNIKEYRDSFSYEKFNFRSNP NITFYVYVSNFSWPIKIQIAFSQHNTIMDLVQFFVTFFSCFLSLLLVAAVVWKIKQTCWA SRRREQLLRERQQMASRPFASVDVALEVGAEQTEFLRGPLEGAPKPIAIEPCAGNRAAVL TVFLCLPRGSSGAPPPGQSGLAIASALIDISQQKASDSKDKTSGVRNRKHLSTRQGTCV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
387
End on reference sequence
4526
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
