LIN54 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  LIN54
Gene Name:  lin-54 homolog (C. elegans)
Original Clone ID:
FLH482318.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00295976
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2247nts
Open reading frame: 1 to 2247

ATGGAGGTGGTGCCAGCTGAGGTGAGTAGTTTGCTTCCAGAGGAAATAATGGACACTGGT
ATAACTTTAGTGGATGATGATAGTATTGAGGCTGTTATTGTTTCATCCCCAATTCCCATG
GAGACAGAACTGGAAGAAATTGTCAACATAAATTCTACTGGTGACTCTACAGCCACGCCC
ATTTCCACGGAACCAATCACAGTGTACAGTAACCACACTAACCAAGTTGCAGTGAATACC
ACAATTACTAAAGCAGATTCTAATACCACAGTGAAACCAGCTTTTCCAAGTGGCCTTCAA
AAACTTGGTGCTCAGACTCCTGTGACTATATCAGCCAATCAGATTATTTTAAACAAAGTA
TCACAGACATCTGATCTTAAACTTGGCAATCAGACCCTTAAACCAGATGGACAGAAGTTA
ATTTTAACAACTTTGGGCAAGTCTGGTTCACCAATTGTTTTAGCACTACCCCATAGCCAA
CTACCCCAGGCTCAGAAAGTTACAACTCAGGCCCAGTCAGGAGATGCTAAGTTACCACCG
CAGCAAATTAAAGTAGTTACCATTGGAGGGAGGCCAGAGGTGAAACCTGTCATTGGTGTC
TCAGCATTGACCCCAGGAAGTCAACTGATTAATACTACAACTCAGCCCTCTGTGTTACAG
ACCCAACAGTTAAAAACAGTACAGATTGCTAAGAAGCCTCGAACGCCAACCTCTGGTCCA
GTAATCACGAAGCTGATCTTTGCAAAACCAATTAATAGTAAAGCAGTTACAGGACAGACA
ACTCAAGTTTCACCACCAGTCATTGCAGGTAGGGTTCTTTCACAGTCTACTCCCGGAACT
CCATCAAAGACCATAACAATATCTGAAAGTGGTGTTATTGGATCAACTTTAAATTCTACA
ACACAGACACCAAATAAAATAGCCATCTCACCTTTGAAATCGCCAAATAAGGCAGTGAAA
TCAACTGTGCAGACCATCACTGTTGGAGGAGTGAGCACATCACAGTTTAAGACAATTATT
CCTCTGGCAACTGCTCCCAATGTCCAGCAGATTCAAGTGCCTGGAAGCAAGTTTCATTAT
GTCCGACTTGTTACTGCCACATCAGCCAGTAGCTCAACCCAGCCAGTTAGTCAGAATCCC
AGTACAAACACTCAGCCTCTTCAGCAAGCAAAGCCAGTGGTTGTTAATACAACCCCAGTG
CGGATGTCAGTTCCAATTGTCTCAGCTCAGGCTGTCAAACAAGTTGTTCCAAAACCAATC
AATCCAACTTCACAAATAGTAACTACTAGCCAGCCACAGCAACGGCTTATCATGCCCGCC
ACACCACTGCCACAGATCCAGCCCAACCTCACTAACCTGCCACCAGGCACTGTCCTGGCA
CCAGCTCCGGGAACAGGGAATGTGGGTTATGCAGTGCTTCCAGCTCAGTATGTTACTCAG
CTACAGCAGTCTTCATATGTATCAATAGCAAGCAACTCTACCTTTACTGGAACATCTGGT
ATCCAGACCCAGGCACGGCTTCCATTCAATGGCATAATCCCATCAGAGTCGGCCAGTCGG
CCCCGAAAGCCCTGTAATTGTACAAAATCACTGTGTTTGAAATTGTATTGTGATTGCTTT
GCAAATGGTGAATTTTGCAACAACTGCAATTGTACTAATTGTTACAACAATTTGGAACAT
GAAAATGAAAGGCAAAAAGCAATAAAGGCATGCCTTGACAGAAATCCAGAAGCCTTTAAG
CCTAAGATAGGGAAAGGAAAGGAGGGAGAATCTGATCGACGTCATAGCAAAGGGTGTAAT
TGCAAACGATCAGGATGTCTTAAAAACTACTGTGAATGCTATGAGGCAAAAATAATGTGT
TCCTCAATATGCAAATGTATTGGCTGTAAGAATTTTGAAGAAAGCCCGGAAAGGAAGACA
TTGATGCATTTGGCAGATGCAGCTGAAGTAAGGGTACAGCAACAAACAGCAGCCAAGACG
AAGTTATCCTCTCAAATTTCAGACTTGCTTACTAGGCCAACACCAGCTTTAAATAGTGGA
GGCGGAAAATTGCCATTTACATTTGTAACTAAGGAAGTAGCTGAAGCCACATGTAATTGC
CTCCTTGCCCAGGCAGAGCAGGCAGACAAGAAGGGAAAATCAAAGGCAGCAGCGGAACGG
ATGATACTTGAGGAATTCGGACGATGTTTGATGAGTGTCATCAACTCTGCAGGAAAGGCA
AAAAGTGCCCCTTGTGCCATGAATTGC

Protein sequence predicted based on nucleotide sequence

MEVVPAEVSSLLPEEIMDTGITLVDDDSIEAVIVSSPIPMETELEEIVNINSTGDSTATP
ISTEPITVYSNHTNQVAVNTTITKADSNTTVKPAFPSGLQKLGAQTPVTISANQIILNKV
SQTSDLKLGNQTLKPDGQKLILTTLGKSGSPIVLALPHSQLPQAQKVTTQAQSGDAKLPP
QQIKVVTIGGRPEVKPVIGVSALTPGSQLINTTTQPSVLQTQQLKTVQIAKKPRTPTSGP
VITKLIFAKPINSKAVTGQTTQVSPPVIAGRVLSQSTPGTPSKTITISESGVIGSTLNST
TQTPNKIAISPLKSPNKAVKSTVQTITVGGVSTSQFKTIIPLATAPNVQQIQVPGSKFHY
VRLVTATSASSSTQPVSQNPSTNTQPLQQAKPVVVNTTPVRMSVPIVSAQAVKQVVPKPI
NPTSQIVTTSQPQQRLIMPATPLPQIQPNLTNLPPGTVLAPAPGTGNVGYAVLPAQYVTQ
LQQSSYVSIASNSTFTGTSGIQTQARLPFNGIIPSESASRPRKPCNCTKSLCLKLYCDCF
ANGEFCNNCNCTNCYNNLEHENERQKAIKACLDRNPEAFKPKIGKGKEGESDRRHSKGCN
CKRSGCLKNYCECYEAKIMCSSICKCIGCKNFEESPERKTLMHLADAAEVRVQQQTAAKT
KLSSQISDLLTRPTPALNSGGGKLPFTFVTKEVAEATCNCLLAQAEQADKKGKSKAAAER
MILEEFGRCLMSVINSAGKAKSAPCAMNC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2628
Start on reference sequence 379
Mutations:
None
Discrepancy:
a1086g; a1697g,K566R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable