MED23 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MED23
Gene Name:  mediator complex subunit 23
Original Clone ID:
FLH482769.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296427
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4077nts
Open reading frame: 1 to 4077

ATGGAGACGCAACTGCAGAGCATTTTCGAAGAGGTGGTGAAAACGGAAGTTATAGAAGAG
GCTTTTCCTGGCATGTTTATGGATACTCCTGAAGATGAGAAAACAAAACTAATTAGCTGT
TTGGGGGCCTTCAGACAGTTTTGGGGTGGTCTTTCTCAGGAGTCTCATGAACAGTGGATC
CAGTGGATTGTTAAGTTTATTCATGGTCAGCATAGTCCTAAAAGAATTTCTTTTCTTTAT
GACTGCTTAGCAATGGCAGTTGAGACTGGTCTCCTTCCACCCAGGCTGGTTTGTGAATCC
CTGATAAACTCTGACACTCTTGAGTGGGAAAGAACACAGCTTTGGGCCTTAACATTTAAA
CTGGTTCGGAAAATAATTGGGGGAGTGGATTACAAGGGTGTTCGAGATCTCTTAAAAGTG
ATTTTGGAGAAGATTTTGACAATTCCTAATACAGTGAGCTCTGCTGTTGTACAGCAGCTT
CTGGCAGCAAGAGAGGTTATAGCATATATCTTGGAAAGAAATGCCTGCTTATTACCAGCC
TATTTTGCAGTCACTGAGATCAGGAAACTGTATCCTGAAGGCAAACTTCCACACTGGTTA
CTTGGAAACCTAGTATCAGACTTTGTGGATACCTTCAGGCCCACAGCAAGGATAAACTCC
ATTTGTGGTCGCTGTAGTCTTCTGCCAGTTGTAAATAATTCGGGTGCCATTTGTAATTCA
TGGAAACTGGATCCTGCTACTCTTCGTTTTCCTTTGAAAGGCCTTTTGCCATATGATAAG
GATCTGTTTGAACCACAGACTGCTTTGTTGAGATATGTATTGGAGCAGCCTTATTCCAGG
GATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTG
GAGGACCAGTTGGTGGATCTGGTTGTTTATGCCATGGAGCGATCTGAGACCGAGGAGAAG
TTTGACGATGGGGGAACAAGCCAACTCCTGTGGCAGCATCTCTCAAGTCAGCTCATTTTC
TTTGTGCTTTTCCAGTTTGCAAGTTTTCCACATATGGTGCTTTCTCTTCATCAGAAGTTA
GCAGGGCGAGGACTGATTAAAGGCAGAGATCATCTTATGTGGGTTCTCCTGCAATTCATT
TCTGGAAGTATTCAGAAAAATGCACTAGCTGATTTTCTCCCTGTGATGAAGCTCTTCGAC
TTGCTATACCCAGAAAAAGAATATATCCCAGTTCCTGATATTAACAAACCCCAGTCAACC
CATGCCTTTGCAATGACCTGTATTTGGATTCATCTCAATAGAAAAGCTCAAAATGACAAC
TCCAAGCTACAGATTCCAATACCTCATTCCCTAAGACTTCACCATGAGTTCCTGCAGCAG
AGTCTAAGAAATAAAAGTTTACAGATGAATGACTATAAGATTGCTCTATTGTGTAATGCA
TACTCTACAAATTCAGAATGTTTTACATTACCCATGGGAGCTCTGGTAGAAACTATTTAT
GGAAATGGAATTATGAGGATACCTCTCCCTGGAACAAACTGTATGGCTTCAGGATCTATT
ACCCCCTTACCTATGAACCTCCTGGATTCACTGACAGTTCATGCCAAAATGAGCCTTATT
CACAGCATTGCAACCAGGGTGATAAAACTTGCTCATGCAAAGTCCAGTGTGGCCTTGGCT
CCAGCCCTAGTGGAAACTTACAGTCGTTTATTGGTCTATATGGAAATAGAGTCTTTGGGC
ATCAAAGGATTTATCAGTCAGCTTTTGCCAACTGTTTTCAAATCACATGCATGGGGGATC
TTACACACACTCCTTGAGATGTTTAGCTACCGGATGCATCATATTCAGCCTCATTACAGA
GTTCAGCTCCTGAGTCATCTTCATACTTTGGCTGCAGTTGCACAAACAAACCAGAACCAG
CTCCATCTTTGTGTCGAGAGCACTGCTCTCAGGCTTATAACAGCATTAGGTAGCTCAGAG
GTACAACCGCAGTTTACACGCTTCCTTAGTGATCCCAAAACAGTGCTCTCAGCAGAATCT
GAAGAACTGAACCGAGCCTTGATATTGACCTTGGCTAGAGCAACTCATGTAACAGATTTT
TTTACAGGCTCTGATTCAATTCAGGGAACTTGGTGTAAAGACATACTTCAGACCATCATG
AGTTTCACTCCTCATAATTGGGCTTCACACACCCTGAGCTGTTTTCCAGGCCCACTACAG
GCATTCTTCAAACAAAATAATGTGCCTCAGGAAAGCCGTTTTAATCTGAAAAAAAATGTG
GAGGAGGAGTATAGGAAGTGGAAGTCAATGAGCAACGAAAACGACATTATTACCCACTTC
TCTATGCAGGGCTCCCCTCCTCTCTTTCTTTGTCTTCTCTGGAAAATGCTCTTGGAAACA
GATCATATTAATCAGATTGGCTATAGAGTATTAGAGAGAATTGGAGCCAGGGCCTTGGTA
GCCCATGTGAGGACATTTGCAGATTTCCTGGTATATGAGTTTTCTACATCAGCAGGGGGT
CAGCAACTCAATAAATGCATTGAAATTCTTAATGACATGGTATGGAAGTATAACATTGTT
ACACTGGACAGATTAATTCTCTGCCTGGCCATGCGTAGTCACGAAGGAAATGAAGCCCAG
GTTTGTTATTTCATAATTCAGTTGCTGTTACTCAAACCAAACGATTTTAGAAATCGAGTA
AGTGACTTTGTGAAGGAAAATTCCCCAGAGCACTGGTTACAGAATGACTGGCACACCAAG
CACATGAATTATCACAAGAAATATCCAGAGAAGTTGTATTTTGAGGGCCTCGCGGAACAG
GTGGATCCTCCTGTACAGATCCAGTCTCCCTATCTGCCCATCTATTTTGGGAATGTGTGT
CTTCGATTCCTTCCAGTATTTGATATAGTAATCCACAGATTTTTAGAGTTGCTTCCGGTA
TCCAAATCACTGGAGACTCTACTGGATCATCTAGGAGGCTTATATAAATTTCATGATCGT
CCAGTGACTTATCTGTATAACACTCTGCACTATTATGAAATGCACCTGAGAGACCGCGCA
TTTCTCAAACGAAAACTCGTCCATGCGATCATTGGCTCTCTGAAGGATAATCGACCGCAG
GGCTGGTGTCTAAGTGACACTTACCTGAAATGCGCTATGAATGCACGAGAGGAAAATCCT
TGGGTTCCAGATGACACCTACTATTGCAGATTGATTGGCAGACTAGTCGATACGATGGCT
GGCAAATCTCCTGGTCCCTTTCCAAACTGTGACTGGAGATTCAATGAGTTTCCCAACCCA
GCTGCCCATGCTCTCCATGTTACTTGTGTGGAGCTCATGGCCTTGGCAGTTTCAGGCAAA
GAAGTTGGGAATGCCCTTCTAAATGTTGTCCTAAAAAGTCAGCCTTTAGTGCCAAGAGAG
AACATTACAGCATGGATGAATGCAATTGGTTTGATCATCACTGCCCTACCAGAGCCATAT
TGGATTGTTCTTCATGATCGAATTGTGAGTGTCATCAGCAGCCCCAGCTTGACGTCTGAA
ACAGAGTGGGTTGGCTATCCATTCCGCCTCTTTGATTTCACTGCCTGTCATCAGTCCTAC
TCTGAGATGAGTTGTAGCTATACGTTAGCTCTTGCACATGCTGTGTGGCACCATTCTAGC
ATCGGACAACTTTCTCTCATTCCAAAGTTTCTTACTGAAGTACTTCTTCCTATAGTGAAG
ACCGAATTCCAGTTGCTTTATGTATACCATCTTGTTGGACCATTTTTACAAAGATTTCAG
CAAGAGAGAACTCGTTGTATGATAGAGATTGGTGTGGCGTTTTATGACATGCTGCTGAAT
GTTGACCAGTGTAGCACCCATTTAAATTACATGGATCCCATCTGTGACTTCCTCTATCAC
ATGAAGTATATGTTTACTGGTGACAGCGTGAAAGAGCAAGTAGAGAAGATTATCTGTAAC
TTAAAACCAGCTTTAAAACTTCGTCTTCGATTCATCACACACATTAGCAAGATGGAGCCA
GCTGCAGTGCCTCCACAAGCCATGAACAGTGGGTCTCCAGCACCTCAGTCTAATCAG

Protein sequence predicted based on nucleotide sequence

METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQWI
QWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFK
LVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPA
YFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNS
WKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVL
EDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKL
AGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQST
HAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNA
YSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLI
HSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGI
LHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE
VQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIM
SFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHF
SMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGG
QQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRV
SDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVC
LRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRA
FLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMA
GKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRE
NITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSY
SEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQ
QERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICN
LKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 135
End on reference sequence 4214
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable