ARHGEF1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ARHGEF1
Gene Name:  Rho guanine nucleotide exchange factor (GEF) 1
Original Clone ID:
FLH482887.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296545
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2637nts
Open reading frame: 1 to 2637

ATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCC
GTCAGCATCATCGGGGCTGAGGATGAGGATTTTGAGAACGAGCTGGAGACAAACTCAGAA
GAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGCCCAGCCCACCTCATG
GCCCTCCTGCAGCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGGTTCTCCGGGTGCCG
GTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACTAGGGCTGACCTCATCTCCGAGGAT
GTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAGCCGTGGGCCGGCAG
CTGGAGGACTTCCGTTCCAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCTGGCC
CAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTG
GCGGAGCGGCTGCTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAA
AAGAGTGCTGCCGTGGTCAACGCCATTGGCCTGTACATGCGCCACCTTGGGGTGCGGACC
AAGAGTGGAGACAAGAAGTCGGGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAACCGG
CGGTCGGACGAGCCTGCCAAGACCAAGAAGGGGCTGAGCAGCATCCTGGATGCCGCCCGC
TGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAGCAGAGGTTGATGCC
GAGAAGCCAGGTGCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCGGAAT
ATCGGGGCTCCTGGGCAGGACACCCCTGGAGTCTCTCTGCACCCTCTGTCCCTGGACAGC
CCAGACCGGGAACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGC
CCAATGAGCCTGGAGTCCTTGGCGCCCCCAGAGAGTACCGACGAGGGGGCCGAAACCGAG
AGCCCCGAGCCTGGAGATGAGGGGGAGCCGGGGCGGTCGGGACTGGAGCTTGAACCAGAA
GAGCCTCCCGGCTGGCGGGAACTCGTCCCCCCAGACACCCTGCACAGCCTGCCCAAGAGC
CAGGTGAAGCGGCAGGAGGTCATCAGCGAGCTGCTGGTGACAGAGGCGGCCCACGTGCGC
ATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTTCCCC
TTGGAGGAGCTGCAGAACATCTTCCCCAGCCTGGACGAGCTCATCGAGGTGCATTCCCTG
TTCCTCGATCGCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGA
GACGTGCTGCTGGCCCGGTTTGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCC
CGCTTCTGCAGCCGCCAGTCATTTGCCTTAGAGCAGCTCAAAGCCAAGCAACGCAAGGAC
CCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGCCGCCCGCGGTGCCGCCGCCTGCAG
CTGAAGGACATGATCCCCACGGAGATGCAGCGGCTGACCAAGTACCCCCTGCTCCTGCAG
AGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGCCGAG
TGCTGCCGGGAAATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTG
AGGCTCAAGGACTATCAGCGGCGCCTGGACTTGTCCCACCTTCGGCAGAGCAGCGACCCT
ATGCTGAGCGAGTTCAAGAACCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCA
CTGACGTGGCGGGTGACTAAGGACAAGGCAGTGGAGGTGCATGTGCTGCTGCTGGACGAC
CTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTGCTGCTCAAGTCCCATAGCCGGACA
CTGACGCCCACGCCCGATGGCAAGACCATGCTGCGGCCCGTGCTGCGGCTCACCTCCGCC
ATGACCCGCGAGGTGGCCACCGATCACAAAGCCTTCTACGTCCTTTTTACCTGGGACCAG
GAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCT
CTCATCACTGAGACTGCCGGATCCCTGAAAGTCCCTGCCCCTGCCTCTCGCCCTAAGCCC
CGGCCCAGCCCGAGCAGCACCCGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGT
GGCCGAGAGACGTCTCCAGCTGATGCCCGGACCGAGAGAATCCTCAGTGACCTCCTGCCC
TTCTGCAGACCAGGCCCCGAGGGCCAGCTCGCTGCCACGGCCCTTCGGAAAGTGCTGTCC
CTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGCCTCCTCGAGATGGG
GATGGGGTCCCAGGGGGCGGCCCCCTGAGCCCAGCACGGACCCAGGAAATCCAGGAGAAC
CTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGC
CTGAGACCCCTCCTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACT

Protein sequence predicted based on nucleotide sequence

MEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELETNSEEQNSQFQSLEQVKRRPAHLM
ALLQHVALQFEPGPLVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQ
LEDFRSKRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTISTDEE
KSAAVVNAIGLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAAR
WNRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDS
PDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPE
EPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFP
LEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISS
RFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQ
SIGQNTEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDP
MLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRT
LTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCA
LITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGNGGRETSPADARTERILSDLLP
FCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQEN
LLSLEETMKQLEELEEEFCRLRPLLSQLGGNSVPQPGCT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2724
Start on reference sequence 85
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable