MARCH4 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MARCH4
Gene Name:  membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase
Original Clone ID:
FLH482929.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296587
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1230nts
Open reading frame: 1 to 1230

ATGCTGATGCCCCTGTGTGGGCTGCTCTGGTGGTGGTGGTGCTGCTGCTCCGGCTGGTAC
TGCTATGGATTGTGTGCCCCAGCCCCCCAGATGTTGCGCCACCAGGGTCTCCTCAAGTGC
CGCTGCCGCATGCTCTTCAATGACCTGAAGGTTTTCTTACTGCGGCGCCCTCCTCAAGCG
CCCCTGCCCATGCACGGCGACCCCCAGCCCCCCGGTTTGGCGGCCAACAACACCCTTCCG
GCTCTGGGCGCCGGGGGGTGGGCAGGCTGGAGGGGCCCCCGAGAAGTGGTGGGCAGGGAG
CCCCCTCCTGTGCCACCTCCACCCCCCTTGCCACCTTCTTCTGTGGAAGATGACTGGGGT
GGCCCAGCCACAGAGCCACCTGCCTCGCTGCTCAGCAGTGCCTCCTCAGATGACTTCTGT
AAGGAGAAGACCGAGGATCGCTACTCACTGGGCAGCAGCTTGGACAGTGGTATGAGGACC
CCACTCTGCCGCATCTGCTTCCAGGGGCCAGAACAGGGGGAGCTGCTGAGCCCATGCCGC
TGTGATGGCTCTGTCAAGTGCACACACCAGCCTTGCCTCATCAAGTGGATCAGCGAGCGG
GGCTGCTGGAGCTGCGAGCTGTGCTACTACAAGTACCACGTCATCGCCATAAGCACAAAA
AATCCTCTGCAGTGGCAGGCCATCTCTCTGACGGTCATTGAGAAGGTTCAGGTTGCAGCC
GCCATCCTGGGCTCCCTCTTCCTCATCGCCAGTATTTCTTGGCTCATCTGGTCAACTTTC
AGCCCCTCGGCAAGATGGCAGCGCCAAGACCTTCTCTTCCAGATCTGCTACGGGATGTAT
GGCTTCATGGACGTGGTGTGCATAGGTCTCATCATCCATGAAGGACCCTCGGTGTACCGC
ATCTTTAAACGGTGGCAGGCTGTCAACCAGCAGTGGAAAGTGCTGAACTATGACAAGACA
AAAGACCTGGAGGATCAAAAGGCAGGAGGCAGGACCAACCCCCGGACCTCCTCATCCACC
CAGGCCAATATCCCCTCCTCGGAAGAGGAGACCGCAGGCACCCCTGCCCCTGAGCAGGGC
CCTGCCCAGGCTGCCGGCCACCCCTCAGGCCCTCTGTCCCATCACCACTGTGCTTATACC
ATCCTGCACATCCTGAGTCACTTGAGACCTCATGAACAGCGAAGTCCCCCAGGCAGCAGC
CGAGAGCTGGTCATGAGAGTCACGACAGTG

Protein sequence predicted based on nucleotide sequence

MLMPLCGLLWWWWCCCSGWYCYGLCAPAPQMLRHQGLLKCRCRMLFNDLKVFLLRRPPQA
PLPMHGDPQPPGLAANNTLPALGAGGWAGWRGPREVVGREPPPVPPPPPLPPSSVEDDWG
GPATEPPASLLSSASSDDFCKEKTEDRYSLGSSLDSGMRTPLCRICFQGPEQGELLSPCR
CDGSVKCTHQPCLIKWISERGCWSCELCYYKYHVIAISTKNPLQWQAISLTVIEKVQVAA
AILGSLFLIASISWLIWSTFSPSARWQRQDLLFQICYGMYGFMDVVCIGLIIHEGPSVYR
IFKRWQAVNQQWKVLNYDKTKDLEDQKAGGRTNPRTSSSTQANIPSSEEETAGTPAPEQG
PAQAAGHPSGPLSHHHCAYTILHILSHLRPHEQRSPPGSSRELVMRVTTV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1429
Start on reference sequence 197
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable