FAM62A (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ESYT1
Gene Name:  extended synaptotagmin-like protein 1
Original Clone ID:
FLH482957.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 26322679
Title: Proteomic mapping of ER_PM junctions identifies STIMATE as a regulator of Ca2+ influx
Authors:
The ORFeome Collaboration
HsCD00296615
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3312nts
Open reading frame: 1 to 3312

ATGGAGCGATCTCCAGGAGAGGGCCCCAGCCCCAGCCCCATGGACCAGCCCTCTGCTCCC
TCCGACCCCACTGACCAGCCCCCCGCTGCTCACGCAAAGCCAGACCCAGGTTCTGGGGGC
CAACCTGCTGGCCCTGGCGCGGCGGGTGAGGCCCTGGCGGTGCTGACTTCATTCGGGAGG
CGGTTGCTGGTGCTGATACCTGTGTATTTGGCCGGGGCAGTGGGACTCAGCGTGGGTTTC
GTGCTCTTCGGCCTCGCCCTCTACCTGGGCTGGCGCCGGGTCCGCGACGAGAAAGAACGG
AGCCTTCGAGCAGCGAGGCAGCTACTGGACGACGAGGAGCAGCTCACTGCGAAAACTCTC
TATATGAGTCATCGAGAGCTACCTGCCTGGGTCAGCTTCCCAGACGTGGAAAAGGCTGAA
TGGCTCAATAAGATTGTGGCCCAGGTCTGGCCCTTCCTGGGCCAGTATATGGAGAAGCTT
CTGGCTGAAACTGTGGCTCCGGCTGTTAGGGGATCTAACCCCCATCTGCAAACATTTACA
TTTACACGAGTGGAACTGGGTGAAAAGCCATTGCGCATCATTGGAGTCAAGGTTCACCCA
GGTCAGAGAAAAGAGCAGATCCTGCTGGACTTGAACATCAGCTATGTAGGTGATGTGCAG
ATTGATGTGGAAGTGAAGAAATATTTTTGCAAAGCAGGAGTCAAGGGCATGCAGCTACAT
GGCGTTTTGCGGGTGATACTGGAGCCACTCATTGGGGACCTTCCCTTCGTGGGGGCTGTG
TCAATGTTCTTCATCCGACGCCCGACCCTAGACATCAACTGGACAGGGATGACCAACCTG
CTGGATATCCCAGGACTTAGCTCACTCTCTGACACCATGATCATGGACTCCATTGCTGCC
TTCCTCGTGTTGCCCAACCGATTACTGGTGCCCCTTGTGCCTGACCTTCAAGATGTGGCT
CAGTTGCGTTCCCCTCTGCCCAGGGGCATTATTCGAATTCACCTGCTGGCTGCTCGAGGG
CTGAGTTCCAAGGACAAATATGTGAAGGGCCTGATTGAGGGCAAGTCAGACCCATATGCA
CTTGTGCGTTTGGGTACCCAGACATTCTGCAGTCGTGTCATTGATGAAGAACTCAACCCA
CAGTGGGGAGAGACTTATGAGGTGATGGTACACGAGGTCCCAGGGCAGGAGATTGAAGTG
GAGGTGTTCGACAAGGATCCAGATAAAGATGACTTTCTGGGCAGAATGAAGCTGGATGTA
GGGAAGGTGTTACAGGCTAGCGTTCTGGATGATTGGTTCCCTCTACAAGGTGGGCAAGGC
CAAGTTCACTTGAGGCTAGAATGGCTGTCACTTTTGTCAGATGCAGAGAAACTGGAGCAG
GTTCTACAGTGGAATTGGGGAGTCTCCTCTCGACCAGATCCCCCGTCAGCTGCCATCTTA
GTTGTCTACCTGGATCGGGCCCAGGATCTTCCTCTGAAGAAGGGGAACAAGGAACCCAAC
CCTATGGTACAACTGTCAATTCAGGATGTGACTCAGGAGAGCAAGGCTGTCTACAGTACC
AACTGCCCAGTGTGGGAGGAAGCGTTCCGGTTCTTCCTACAAGACCCTCAAAGCCAGGAG
CTCGATGTGCAAGTGAAGGATGATTCCAGGGCCCTGACTTTAGGAGCACTGACGCTGCCT
CTGGCCCGCCTGCTGACTGCCCCAGAACTCATCCTGGACCAGTGGTTCCAGCTCAGCAGC
TCTGGTCCAAACTCCAGACTCTATATGAAACTAGTCATGAGGATCCTGTACTTGGATTCA
TCAGAAATATGCTTCCCCACGGTGCCTGGTTGTCCTGGTGCTTGGGACGTGGACAGTGAG
AATCCCCAGAGAGGCAGCAGTGTGGATGCCCCACCTCGACCCTGTCACACGACTCCTGAT
AGCCAGTTTGGGACTGAGCATGTGCTTCGGATCCATGTATTAGAGGCCCAGGACCTGATT
GCCAAAGACCGTTTCTTGGGGGGACTGGTGAAGGGCAAGTCAGACCCCTATGTCAAACTA
AAGTTGGCAGGACGAAGCTTCCGGAGCCATGTTGTTCGGGAAGATCTCAATCCCCGCTGG
AATGAGGTTTTTGAGGTGATCGTCACATCAGTTCCAGGCCAAGAGCTAGAGGTTGAAGTC
TTTGACAAGGACTTGGACAAGGATGATTTTCTGGGCAGGTGTAAAGTGCGTCTCACCACA
GTCTTAAACAGTGGCTTCCTTGATGAGTGGCTGACCCTGGAGGATGTCCCATCTGGCCGC
CTGCACTTGCGCCTGGAGCGTCTCACCCCCCGTCCCACTGCTGCTGAGTTAGAGGAGGTG
CTGCAGGTGAATAGTTTGATCCAGACTCAGAAGAGTGCGGAGCTGGCTGCGGCCCTGCTA
TCCATCTATATGGAGCGGGCAGAGGACCTCCCGCTGCGAAAAGGCACCAAGCACCTCAGC
CCTTATGCTACTCTCACTGTGGGAGATAGTTCTCATAAAACCAAGACTATTTCGCAAACT
TCAGCCCCTGTCTGGGATGAGAGTGCCTCCTTTCTCATCAGGAAACCACACACTGAGAGC
CTAGAGTTGCAGGTTCGGGGTGAGGGCACTGGCGTGCTGGGCTCATTATCCCTGCCCCTC
TCAGAGCTCCTCGTGGCTGACCAGCTCTGCTTGGACCGCTGGTTTACACTCAGCAGTGGT
CAGGGGCAGGTGCTACTGAGAGCACAGCTAGGGATCCTGGTGTCCCAGCACTCGGGAGTG
GAAGCTCATAGCCACAGCTACAGCCACAGCTCCTCATCGCTGAGTGAAGAACCAGAGCTC
TCGGGGGGACCCCCTCACATCACCTCCTCAGCCCCAGAGCTCCGGCAGCGCCTAACACAT
GTTGACAGTCCCCTTGAGGCTCCAGCCGGGCCTCTGGGCCAGGTGAAACTGACTCTGTGG
TACTACAGTGAAGAACGAAAGCTGGTCAGCATTGTTCATGGTTGCCGGTCCCTTCGACAG
AATGGACGTGATCCTCCTGATCCCTATGTGTCACTGTTGCTACTGCCAGACAAGAACCGA
GGCACCAAGAGGAGGACCTCACAGAAGAAGAGGACCCTGAGTCCTGAATTTAATGAACGG
TTTGAGTGGGAACTCCCCCTGGATGAGGCCCAGAGACGAAAGCTGGATGTCTCTGTCAAG
TCTAATTCCTCCTTCATGTCAAGAGAGCGTGAGCTGCTGGGGAAGGTGCAGCTGGACCTA
GCTGAGACAGACCTTTCCCAGGGTGTAGCCCGGTGGTATGACCTGATGGACAACAAGGAC
AAGGGCAGCTCC

Protein sequence predicted based on nucleotide sequence

MERSPGEGPSPSPMDQPSAPSDPTDQPPAAHAKPDPGSGGQPAGPGAAGEALAVLTSFGR
RLLVLIPVYLAGAVGLSVGFVLFGLALYLGWRRVRDEKERSLRAARQLLDDEEQLTAKTL
YMSHRELPAWVSFPDVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAPAVRGSNPHLQTFT
FTRVELGEKPLRIIGVKVHPGQRKEQILLDLNISYVGDVQIDVEVKKYFCKAGVKGMQLH
GVLRVILEPLIGDLPFVGAVSMFFIRRPTLDINWTGMTNLLDIPGLSSLSDTMIMDSIAA
FLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSKDKYVKGLIEGKSDPYA
LVRLGTQTFCSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVFDKDPDKDDFLGRMKLDV
GKVLQASVLDDWFPLQGGQGQVHLRLEWLSLLSDAEKLEQVLQWNWGVSSRPDPPSAAIL
VVYLDRAQDLPLKKGNKEPNPMVQLSIQDVTQESKAVYSTNCPVWEEAFRFFLQDPQSQE
LDVQVKDDSRALTLGALTLPLARLLTAPELILDQWFQLSSSGPNSRLYMKLVMRILYLDS
SEICFPTVPGCPGAWDVDSENPQRGSSVDAPPRPCHTTPDSQFGTEHVLRIHVLEAQDLI
AKDRFLGGLVKGKSDPYVKLKLAGRSFRSHVVREDLNPRWNEVFEVIVTSVPGQELEVEV
FDKDLDKDDFLGRCKVRLTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTPRPTAAELEEV
LQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTKHLSPYATLTVGDSSHKTKTISQT
SAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSLSLPLSELLVADQLCLDRWFTLSSG
QGQVLLRAQLGILVSQHSGVEAHSHSYSHSSSSLSEEPELSGGPPHITSSAPELRQRLTH
VDSPLEAPAGPLGQVKLTLWYYSEERKLVSIVHGCRSLRQNGRDPPDPYVSLLLLPDKNR
GTKRRTSQKKRTLSPEFNERFEWELPLDEAQRRKLDVSVKSNSSFMSRERELLGKVQLDL
AETDLSQGVARWYDLMDNKDKGSS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3365
Start on reference sequence 51
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable