PDS5A (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PDS5A
Gene Name:  PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
Original Clone ID:
FLH483048.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296706
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3891nts
Open reading frame: 1 to 3891

ATGATCAAACGCCTGAAGATGGTAGTGAAAACCTTTATGGATATGGATCAGGACTCAGAA
GATGAAAAACAGCAGTATCTCCCACTAGCCTTGCATCTTGCATCTGAATTCTTCCTCAGG
AACCCCAATAAAGATGTGCGTCTCCTTGTAGCATGTTGTTTGGCTGATATCTTTCGTATC
TATGCCCCAGAAGCTCCATATACTTCCCATGATAAACTTAAGGACATATTTTTGTTTATT
ACCAGACAATTAAAAGGTTTGGAGGATACAAAGAGTCCACAGTTTAATAGATACTTTTAT
TTATTAGAGAATTTAGCTTGGGTTAAATCATATAACATCTGCTTTGAATTGGAAGATTGC
AATGAAATTTTTATTCAGCTTTTTAGAACTCTCTTCTCAGTGATCAACAATAGCCACAAT
AAGAAGGTACAAATGCACATGCTAGATTTGATGAGTTCTATCATCATGGAAGGTGATGGA
GTTACTCAAGAATTATTGGACTCCATTCTTATTAACCTCATTCCTGCACATAAGAACTTA
AATAAACAGTCCTTTGACCTTGCAAAAGTGCTATTGAAAAGAACAGTCCAGACTATTGAG
GCATGCATTGCTAATTTTTTCAATCAAGTCCTGGTGCTGGGAAGATCATCAGTAAGTGAT
TTGTCAGAACATGTATTTGATCTGATTCAGGAACTTTTTGCTATAGATCCTCATTTATTA
TTATCCGTCATGCCACAGCTTGAATTCAAACTAAAGAGCAATGATGGAGAAGAGCGATTA
GCTGTTGTTCGACTTCTAGCTAAATTGTTTGGCTCCAAAGATTCTGATTTGGCAACACAG
AATCGTCCTCTTTGGCAATGTTTTCTTGGACGATTTAATGATATTCATGTTCCTGTGAGA
TTAGAAAGTGTGAAATTTGCCAGTCATTGTTTAATGAATCACCCAGATTTAGCGAAGGAT
CTCACAGAATATTTAAAGGTTAGATCACATGATCCAGAAGAAGCTATTCGTCATGATGTC
ATTGTTACTATAATAACAGCTGCCAAGAGGGACCTGGCCTTAGTAAATGATCAGCTGCTT
GGCTTTGTAAGGGAAAGAACACTGGATAAACGGTGGCGAGTAAGAAAAGAAGCTATGATG
GGTCTGGCTCAGCTTTATAAGAAATACTGTCTTCATGGTGAGGCAGGAAAGGAAGCTGCA
GAGAAAGTCAGCTGGATAAAGGACAAACTTCTGCATATTTATTATCAGAACAGCATTGAC
GACAAACTGTTGGTAGAGAAAATCTTTGCTCAGTATCTTGTCCCCCACAACCTGGAAACA
GAAGAGAGAATGAAATGCTTATATTACTTATATGCTAGTTTGGATCCAAATGCTGTAAAA
GCTCTCAACGAAATGTGGAAGTGTCAGAACATGCTTCGGAGCCATGTACGCGAACTATTG
GATTTGCACAAGCAGCCTACATCAGAGGCTAACTGTTCTGCCATGTTTGGAAAACTGATG
ACCATAGCAAAGAATTTGCCTGACCCCGGGAAAGCACAAGATTTTGTGAAGAAATTTAAC
CAGGTTCTCGGCGATGATGAGAAACTTCGGTCTCAGTTGGAGTTATTAATTAGCCCAACC
TGTTCTTGCAAACAAGCAGATATTTGTGTGAGAGAAATAGCCCGGAAACTTGCAAATCCT
AAGCAACCAACAAATCCTTTTCTAGAGATGGTCAAATTTCTGTTGGAAAGAATCGCACCT
GTGCACATTGATTCAGAAGCCATAAGTGCACTAGTGAAATTGATGAATAAGTCAATAGAG
GGGACAGCAGATGATGAAGAGGAGGGTGTAAGTCCAGATACAGCTATCCGTTCAGGACTT
GAACTTCTTAAGGTTCTGTCTTTTACACATCCTACCTCGTTCCACTCTGCAGAGACATAT
GAGTCCTTGTTACAGTGCCTAAGAATGGAGGATGACAAGGTAGCAGAAGCTGCTATTCAA
ATTTTTAGAAATACAGGTCACAAAATAGAAACAGACCTTCCCCAGATACGATCGACCTTA
ATTCCCATTTTACATCAAAAAGCAAAGAGGGGTACTCCACACCAAGCAAAACAGGCTGTG
CACTGTATACACGCCATATTCACAAATAAAGAAGTCCAGCTTGCACAGATTTTTGAGCCA
CTCAGTAGGAGTCTGAATGCTGATGTGCCAGAACAACTTATAACTCCATTAGTTTCATTG
GGCCACATTTCTATGTTAGCACCAGATCAGTTTGCTTCCCCAATGAAATCTGTAGTAGCA
AATTTTATTGTGAAAGATCTGCTAATGAATGACAGGTCAACAGGTGAAAAGAATGGAAAA
CTGTGGTCTCCAGATGAAGAGGTTTCCCCTGAAGTACTAGCAAAGGTACAGGCAATTAAA
CTTCTGGTAAGGTGGCTGTTGGGTATGAAAAACAACCAGTCTAAATCTGCCAATTCAACC
CTTCGGTTATTATCAGCGATGTTGGTTAGTGAGGGTGACCTGACAGAGCAAAAGAGGATC
AGTAAATCTGATATGTCTCGCTTGCGATTAGCTGCTGGTAGTGCCATAATGAAGCTTGCT
CAGGAACCTTGTTACCATGAAATTATTACCCCAGAACAGTTTCAGCTCTGTGCACTTGTT
ATTAATGATGAGTGTTACCAAGTAAGGCAGATATTTGCTCAGAAGCTGCATAAGGCACTT
GTGAAGTTACTGCTCCCATTGGAGTATATGGCGATCTTTGCCTTGTGTGCCAAAGATCCT
GTGAAGGAGAGAAGAGCACACGCACGACAATGTTTACTGAAAAATATCAGTATACGCAGG
GAATACATTAAGCAGAATCCTATGGCTACTGAGAAATTATTATCACTGTTGCCTGAATAT
GTAGTTCCATACATGATTCACCTGCTAGCCCATGATCCAGATTTTACAAGATCACAAGAT
GTTGATCAGCTTCGTGATATCAAAGAGTGCCTATGGTTCATGCTTGAAGTTTTAATGACA
AAGAATGAAAACAATAGCCATGCCTTTATGAAGAAGATGGCAGAGAACATCAAGTTAACC
AGAGATGCCCAGTCTCCAGATGAATCCAAGACAAATGAAAAACTGTATACAGTATGTGAT
GTGGCTCTCTGTGTTATAAATAGTAAAAGTGCTTTGTGCAATGCAGATTCACCAAAGGAC
CCAGTCCTCCCAATGAAATTTTTTACACAACCTGAAAAGGACTTCTGTAACGATAAGAGT
TATATTTCAGAAGAGACAAGAGTACTTCTGTTAACAGGAAAGCCAAAGCCTGCTGGAGTA
CTAGGTGCAGTAAATAAGCCTTTATCAGCAACGGGAAGGAAACCCTATGTTAGAAGCACT
GGCACTGAGACTGGAAGCAATATTAATGTAAATTCAGAGCTGAACCCTTCAACCGGAAAT
CGATCAAGGGAACAGAGTTCAGAGGCAGCAGAAACTGGAGTTAGTGAAAATGAAGAGAAC
CCTGTGAGGATTATTTCAGTCACACCTGTAAAGAATATTGACCCAGTAAAGAATAAGGAA
ATTAATTCTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAGAACA
GTAACAGCAGCTGGTGCAGAGAATATCCAACAAAAAACAGATGAGAAAGTAGATGAATCG
GGACCTCCCGCCCCTTCCAAACCCAGGAGAGGACGTCGACCCAAGTCTGAATCTCAGGGC
AATGCTACCAAAAATGATGATCTAAATAAACCTATTAACAAGGGAAGGAAGAGAGCTGCA
GTGGGTCAGGAGAGCCCTGGGGGTTTGGAAGCAGGTAATGCCAAAGCACCCAAACTGCAA
GATTTAGCCAAAAAGGCAGCACCAGCAGAAAGACAAATTGACTTACAAAGG

Protein sequence predicted based on nucleotide sequence

MIKRLKMVVKTFMDMDQDSEDEKQQYLPLALHLASEFFLRNPNKDVRLLVACCLADIFRI
YAPEAPYTSHDKLKDIFLFITRQLKGLEDTKSPQFNRYFYLLENLAWVKSYNICFELEDC
NEIFIQLFRTLFSVINNSHNKKVQMHMLDLMSSIIMEGDGVTQELLDSILINLIPAHKNL
NKQSFDLAKVLLKRTVQTIEACIANFFNQVLVLGRSSVSDLSEHVFDLIQELFAIDPHLL
LSVMPQLEFKLKSNDGEERLAVVRLLAKLFGSKDSDLATQNRPLWQCFLGRFNDIHVPVR
LESVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVTIITAAKRDLALVNDQLL
GFVRERTLDKRWRVRKEAMMGLAQLYKKYCLHGEAGKEAAEKVSWIKDKLLHIYYQNSID
DKLLVEKIFAQYLVPHNLETEERMKCLYYLYASLDPNAVKALNEMWKCQNMLRSHVRELL
DLHKQPTSEANCSAMFGKLMTIAKNLPDPGKAQDFVKKFNQVLGDDEKLRSQLELLISPT
CSCKQADICVREIARKLANPKQPTNPFLEMVKFLLERIAPVHIDSEAISALVKLMNKSIE
GTADDEEEGVSPDTAIRSGLELLKVLSFTHPTSFHSAETYESLLQCLRMEDDKVAEAAIQ
IFRNTGHKIETDLPQIRSTLIPILHQKAKRGTPHQAKQAVHCIHAIFTNKEVQLAQIFEP
LSRSLNADVPEQLITPLVSLGHISMLAPDQFASPMKSVVANFIVKDLLMNDRSTGEKNGK
LWSPDEEVSPEVLAKVQAIKLLVRWLLGMKNNQSKSANSTLRLLSAMLVSEGDLTEQKRI
SKSDMSRLRLAAGSAIMKLAQEPCYHEIITPEQFQLCALVINDECYQVRQIFAQKLHKAL
VKLLLPLEYMAIFALCAKDPVKERRAHARQCLLKNISIRREYIKQNPMATEKLLSLLPEY
VVPYMIHLLAHDPDFTRSQDVDQLRDIKECLWFMLEVLMTKNENNSHAFMKKMAENIKLT
RDAQSPDESKTNEKLYTVCDVALCVINSKSALCNADSPKDPVLPMKFFTQPEKDFCNDKS
YISEETRVLLLTGKPKPAGVLGAVNKPLSATGRKPYVRSTGTETGSNINVNSELNPSTGN
RSREQSSEAAETGVSENEENPVRIISVTPVKNIDPVKNKEINSDQATQGNISSDRGKKRT
VTAAGAENIQQKTDEKVDESGPPAPSKPRRGRRPKSESQGNATKNDDLNKPINKGRKRAA
VGQESPGGLEAGNAKAPKLQDLAKKAAPAERQIDLQR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 30
End on reference sequence 3923
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable