RAPGEF1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  RAPGEF1
Gene Name:  Rap guanine nucleotide exchange factor (GEF) 1
Original Clone ID:
FLH483124.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296782
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3282nts
Open reading frame: 1 to 3282

ATGAGCGGCGGCCTCGGCCTCCGGCGCAGCCCGGAAATGTCCGGCAAGATCGAGAAAGCA
GACTCTCAGCGTTCTCATCTCTCTTCCTTCACCATGAAGCTGATGGACAAATTCCACTCA
CCCAAAATCAAGAGAACGCCATCAAAGAAGGGAAAACCAGCTGAGGTGTCCGTAAAGATT
CCAGAGAAGCCTGTGAACAAAGAGGCAACAGACAGATTTCTACCAGAGGGCTACCCTCTC
CCCTTGGATCTGGAGCAGCAGGCAGTAGAATTTATGTCCACCAGTGCTGTGGCTTCCAGG
TCTCAAAGGCAGAAGAACCTGAGCTGGCTGGAGGAGAAAGAGAAGGAAGTTGTCAGTGCC
CTGCGCTACTTTAAGACCATTGTGGACAAAATGGCAATTGATAAGAAGGTACTGGAGATG
CTTCCAGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGTGCAGAACGATCCT
CGAATTCAGCACAGCTCAGCCCTCTCTTCCTGCTATAGCCGAGTGTACCAAAGCCTCGCC
AACCTCATTCGCTGGTCTGACCAAGTGATGCTGGAAGGCGTGAACTCAGAAGACAAGGAG
ATGGTGACGACTGTGAAGGGGGTCATCAAGGCTGTGCTGGATGGAGTGAAGGAGCTGGTC
AGGCTCACCATCGAGAAGCAGGGACGTCCGTCTCCGACGAGCCCCGTGAAGCCCAGTTCC
CCTGCCAGCAAGCCTGATGGCCCAGCAGAGCTCCCCCTGACAGACCGCGAGGTAGAGATC
CTAAACAAGACGACTGGGATGTCACAGTCAACTGAGCTCCTCCCAGATGCCACGGATGAA
GAGGTCGCGCCCCCCAAGCCTCCTCTGCCTGGCATTCGGGTGGTTGATAATAGTCCTCCA
CCAGCATTGCCACCCAAGAAAAGACAGTCGGCGCCGTCCCCTACCCGAGTGGCTGTGGTG
GCCCCCATGAGCCGAGCCACCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGAT
TTTGATGTTGACTGTTACGCACAGAGGCGACTGTCAGGAGGCAGCCACTCATATGGTGGA
GAGTCGCCCCGCCTCTCCCCCTGCAGCAGCATAGGCAAGCTCAGCAAGTCAGACGAGCAG
CTGTCCTCTCTGGACAGGGACAGTGGGCAGTGCTCCCGGAACACAAGCTGTGAAACACTA
GACCACTATGATCCCGACTATGAATTCCTCCAGCAAGACCTCTCTAACGCAGACCAGATA
CCTCAGCAGACGGCCTGGAACCTTAGCCCGTTGCCAGAGTCTTTGGGGGAGTCTGGGTCT
CCATTTCTTGGCCCTCCTTTCCAGCTGCCTCTTGGCGGCCATCCCCAGCCAGACGGACCT
CTGGCCCCAGGGCAGCAGACAGATACGCCACCTGCTCTCCCCGAGAAGAAGCGCAGGAGC
GCAGCCTCCCAGACGGCGGACGGCTCTGGCTGCAGGGTGTCCTACGAGCGGCATCCCTCG
CAGTATGACAACATCTCTGGGGAGGACCTGCAGAGCACAGCCCCGATCCCATCCGTCCCC
TACGCGCCCTTTGCTGCTATTCTGCCCTTTCAGCATGGAGGTTCCTCAGCCCCTGTCGAA
TTTGTGGGTGATTTTACTGCTCCTGAGTCAACCGGTGACCCAGAAAAACCACCTCCTCTA
CCAGAGAAGAAAAACAAACACATGCTGGCCTACATGCAGTTGCTGGAGGACTACTCGGAG
CCGCAGCCCTCTATGTTCTACCAGACGCCACAGAACGAGCACATCTACCAGCAGAAGAAC
AAGCTCCTCATGGAGGTATACGGCTTCAGCGACTCCTTCAGTGGGGTGGACTCCGTGCAG
GAGCTGGCCCCGCCGCCCGCCCTACCCCCCAAGCAGCGGCAGCTGGAGCCACCGGCTGGG
AAAGACGGACATCCCAGAGATCCCTCAGCGGTCAGCGGCGTCCCTGGGAAGGACAGCAGA
GACGGCAGTGAGAGGGCCCCAAAGTCACCAGATGCTCTGGAGTCGGCTCAGTCGGAGGAG
GAAGTGGACGAGCTGTCCCTCATTGACCACAACGAAATTATGTCCAGGCTGACGCTCAAG
CAGGAGGGTGATGACGGGCCGGACGTCCGCGGAGGATCTGGGGACATCTTACTGGTCCAT
GCTACTGAGACTGACAGGAAAGATTTGGTGTTGTACTGCGAGGCATTCCTGACCACCTAC
AGGACCTTCATCTCCCCAGAGGAGCTCATCAAGAAGCTGCAGTACAGATATGAGAAATTC
TCTCCCTTTGCCGACACATTCAAGAAGCGCGTCAGCAAGAACACGTTCTTCGTGCTGGTA
CGGGTGGTGGATGAGCTCTGCCTGGTGGAGTTGACAGAAGAGATCCTGAAGCTGCTGATG
GAACTGGTCTTCCGCCTGGTGTGCAATGGGGAGCTGAGCCTGGCCCGTGTGCTCCGGAAG
AACATCCTGGACAAGGTGGACCAGAAGAAGCTACTCAGGTGTGCCACCTCCAGCCAGCCC
CTGGCAGCCCGGGGGGTAGCAGCCAGGCCGGGGACCTTGCACGACTTTCACAGCCATGAG
ATAGCGGAGCAGCTAACGCTGCTGGATGCTGAGCTCTTCTATAAAATAGAGATTCCTGAG
GTTTTGCTTTGGGCAAAAGAGCAGAATGAGGAGAAGAGCCCCAACTTGACCCAGTTCACG
GAGCACTTCAACAACATGTCCTACTGGGTCCGGTCCATAATCATGTTACAGGAAAAGGCC
CAGGACAGGGAACGGCTGCTCTTGAAGTTCATCAAGATCATGAAGCACTTGCGGAAGCTG
AATAACTTCAACTCCTACTTGGCCATCCTCTCTGCCCTGGACTCGGCGCCCATCCGCAGG
CTGGAGTGGCAGAAGCAGACTTCAGAGGGCCTGGCCGAGTACTGCACACTGATCGACAGC
TCGTCCTCCTTCCGAGCCTACCGGGCCGCCCTCTCGGAGGTGGAACCGCCGTGCATCCCG
TACCTGGGGCTGATCCTGCAGGACCTGACCTTCGTTCACCTGGGAAACCCAGACTACATC
GACGGGAAAGTGAACTTCTCCAAGCGGTGGCAGCAGTTCAACATCCTCGACAGCATGCGC
TGCTTCCAGCAGGCGCACTATGACATGCGGAGGAACGACGACATTATAAACTTCTTCAAT
GACTTCAGTGACCACCTGGCTGAGGAGGCCCTATGGGAACTGTCTCTGAAAATTAAACCC
AGGAACATAACAAGGAGAAAAACAGACCGGGAAGAGAAGACC

Protein sequence predicted based on nucleotide sequence

MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKI
PEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSA
LRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLA
NLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSS
PASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPP
PALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGG
ESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQI
PQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRS
AASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVE
FVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKN
KLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSR
DGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVH
ATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLV
RVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQP
LAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFT
EHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRR
LEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYI
DGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKP
RNITRRKTDREEKT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 16
End on reference sequence 3300
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable