PHC3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PHC3
Gene Name:  polyhomeotic homolog 3 (Drosophila)
Original Clone ID:
FLH483142.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296800
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2952nts
Open reading frame: 23 to 2974

GTACAAAAAAGCAGGCTCCACCATGGATACTGAACCAAACCCGGGAACATCTTCTGTGTC
AACAACAACCAGCAGTACCACCACCACCACCATCACCACTTCCTCCTCTCGAATGCAGCA
GCCACAGATCTCTGTCTACAGTGGTTCAGACCGACATGCTGTACAGGTAATTCAACAGGC
ATTGCATCGGCCCCCCAGCTCAGCTGCTCAGTACCTTCAGCAAATGTATGCAGCCCAACA
ACAGCACTTGATGCTGCATACTGCAGCTCTTCAGCAGCAGCATTTAAGCAGCTCCCAGCT
TCAGAGCCTTGCTGCTGTTCAGGCAAGTTTGTCCAGTGGAAGACCATCTACATCTCCCAC
AGGAAGTGTCACACAGCAGTCAAGTATGTCCCAAACGTCTATCAACCTCTCCACTTCTCC
TACACCTGCACAGTTAATAAGCCGTTCCCAGGCTTCCAGTTCTACCAGCGGCAGTATTAC
CCAACAGACTATGTTACTAGGGAGTACTTCCCCTACCCTAACGGCAAGCCAAGCTCAAAT
GTATCTCCGAGCTCAAATGCTGATTTTCACACCCGCTACCACTGTGGCTGCTGTACAGTC
TGACATTCCTGTTGTCTCGTCGTCATCGTCATCTTCCTGTCAGTCTGCAGCTACTCAGGT
TCAGAATTTAACATTACGCAGCCAGAAGTTGGGTGTATTATCTAGCTCACAGAATGGTCC
ACCAAAAAGCACTAGTCAAACTCAGTCATTGACAATTTGTCATAACAAAACAACAGTGAC
CAGTTCTAAAATCAGCCAACGAGATCCTTCTCCAGAAAGTAATAAGAAAGGAGAGAGCCC
AAGCCTGGAATCACGAAGCACAGCTGTCACCCGGACATCAAGTATTCACCAGTTAATAGC
ACCAGCTTCATATTCTCCAATTCAGCCTCATTCTCTAATAAAACATCAGCGGATTCCTCT
TCATTCACCACCTTCCAAAGTTTCCCATCATCAGCTGATATTACAACAGCAGCAACAGCA
AATTCAGCCAATCACACTTCAGAATTCAACTCAAGACCCACCCCCATCCCAGCACTGTAT
ACCACTCCAGAACCATGGCCTTCCTCCAGCTCCCAGTAATGCCCAGTCACAGCATTGTTC
ACCGATTCAGAGTCATCCCTCTCCTTTAACAGTGTCTCCTAATCAGTCACAGTCAGCACA
GCAGTCTGTAGTGGTGTCTCCTCCACCACCTCATTCACCAAGTCAGTCTCCTACTATAAT
TATTCATCCACAAGCACTTATTCAGCCACACCCTCTTGTGTCATCAGCTCTCCAGCCAGG
GCCAGATTTGCAGCAGTCCACTGCTAATCAGGTGCAAGCTACAGCACAGTTGAATCTTCC
ATCCCATCTTCCACTTCCAGCTTCCCCTGTTGTACACATTGGCCCAGTTCAGCAGTCTGC
CTTGGTATCCCCAGGCCAGCAGATTGTCTCTCCATCACACCAGCAATATTCATCCCTGCA
GTCCTCTCCAATCCCAATTGCAAGTCCTCCACAGATGTCGACATCTCCTCCAGCTCAGAT
TCCACCACTGCCCTTGCAGTCTATGCAGTCTTTACAAGTGCAGCCTGAAATTCTGTCCCA
GGGCCAGGTTTTGGTGCAGAATGCTTTGGTGTCAGAAGAGGAACTTCCAGCTGCAGAAGC
TTTGGTCCAGTTGCCATTTCAGACTCTTCCTCCTCCACAGACTGTTGCGGTAAACCTACA
AGTGCAACCACCAGCACCTGTTGATCCACCAGTGGTTTATCAGGTAGAAGATGTGTGTGA
AGAAGAAATGCCAGAAGAGTCAGATGAATGTGTCCGGATGGATAGAACCCCACCACCACC
CACTTTGTCTCCAGCAGCTATAACAGTGGGGAGAGGAGAAGATTTGACTTCTGAACATCC
TTTGTTAGAGCAAGTGGAATTACCTGCTGTGGCATCAGTCAGTGCTTCAGTAATTAAATC
TCCATCAGATCCCTCACATGTTTCTGTTCCACCACCTCCATTGTTACTTCCAGCTGCCAC
CACAAGGAGTAACAGTACATCTATGCACAGTAGCATTCCCAGTATAGAGAACAAACCTCC
ACAGGCTATTGTTAAACCACAGATCCTAACCCATGTTATTGAAGGCTTTGTGATTCAGGA
GGGATTGGAGCCATTTCCTGTGAGTCGTTCCTCTTTGCTAATAGAACAGCCTGTGAAAAA
ACGGCCTCTTTTGGATAATCAGGTGATAAATTCAGTGTGTGTTCAGCCAGAGCTACAGAA
TAATACAAAACATGCGGATAATTCATCTGACACAGAGATGGAAGACATGATTGCTGAAGA
GACATTAGAAGAAATGGACAGTGAGTTGCTCAAGTGTGAATTCTGTGGGAAAATGGGATA
TGCTAATGAATTTTTGCGGTCAAAACGATTCTGCACTATGTCATGTGCCAAAAGGTACAA
TGTTAGCTGTTCTAAAAAATTTGCACTTAGTCGTTGGAATCGTAAGCCTGATAACCAAAG
TCTTGGGCATCGTGGCCGTCGTCCAAGTGGCCCTGATGGGGCAGCGAGAGAACATATCCT
TAGGCAGCTTCCAATTACTTATCCATCTGCAGAAGAAGACTTGGCTTCTCATGAAGATTC
TGTGCCATCTGCTATGACAACTCGTCTGCGCAGGCAGAGCGAGCGGGAAAGAGAACGTGA
GCTTCGGGATGTGAGAATTCGGAAAATGCCTGAGAACAGTGACTTGCTACCAGTTGCACA
AACAGAGCCATCTATATGGACAGTTGATGATGTCTGGGCCTTCATCCATTCTTTGCCTGG
CTGCCAGGATATCGCAGATGAATTCAGAGCACAGGAGATTGATGGACAGGCCCTTCTCTT
GCTGAAAGAAGACCATCTCATGAGTGCAATGAATATCAAGCTAGGCCCAGCCCTGAAGAT
CTGTGCACGCATCAACTCTCTGAAGGAATCTTGAATCCACCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MDTEPNPGTSSVSTTTSSTTTTTITTSSSRMQQPQISVYSGSDRHAVQVIQQALHRPPSS
AAQYLQQMYAAQQQHLMLHTAALQQQHLSSSQLQSLAAVQASLSSGRPSTSPTGSVTQQS
SMSQTSINLSTSPTPAQLISRSQASSSTSGSITQQTMLLGSTSPTLTASQAQMYLRAQML
IFTPATTVAAVQSDIPVVSSSSSSSCQSAATQVQNLTLRSQKLGVLSSSQNGPPKSTSQT
QSLTICHNKTTVTSSKISQRDPSPESNKKGESPSLESRSTAVTRTSSIHQLIAPASYSPI
QPHSLIKHQRIPLHSPPSKVSHHQLILQQQQQQIQPITLQNSTQDPPPSQHCIPLQNHGL
PPAPSNAQSQHCSPIQSHPSPLTVSPNQSQSAQQSVVVSPPPPHSPSQSPTIIIHPQALI
QPHPLVSSALQPGPDLQQSTANQVQATAQLNLPSHLPLPASPVVHIGPVQQSALVSPGQQ
IVSPSHQQYSSLQSSPIPIASPPQMSTSPPAQIPPLPLQSMQSLQVQPEILSQGQVLVQN
ALVSEEELPAAEALVQLPFQTLPPPQTVAVNLQVQPPAPVDPPVVYQVEDVCEEEMPEES
DECVRMDRTPPPPTLSPAAITVGRGEDLTSEHPLLEQVELPAVASVSASVIKSPSDPSHV
SVPPPPLLLPAATTRSNSTSMHSSIPSIENKPPQAIVKPQILTHVIEGFVIQEGLEPFPV
SRSSLLIEQPVKKRPLLDNQVINSVCVQPELQNNTKHADNSSDTEMEDMIAEETLEEMDS
ELLKCEFCGKMGYANEFLRSKRFCTMSCAKRYNVSCSKKFALSRWNRKPDNQSLGHRGRR
PSGPDGAAREHILRQLPITYPSAEEDLASHEDSVPSAMTTRLRRQSERERERELRDVRIR
KMPENSDLLPVAQTEPSIWTVDDVWAFIHSLPGCQDIADEFRAQEIDGQALLLLKEDHLM
SAMNIKLGPALKICARINSLKES*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3001
Start on reference sequence 50
Mutations:
None
Discrepancy:
a929g,Q310R; a1303g,N435D;
 t2493c
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable