Gene:
Gene Symbol:
ABI3BP
Gene Name:
ABI family, member 3 (NESH) binding protein
Original Clone ID:
FLH483304.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296962
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCGAGGTGGCAAATGCAACATGCTCTCCAGTTTGGGGTGTCTACTTCTCTGTGGAAGT
ATTACACTAGCCCTGGGAAATGCACAGAAATTGCCAAAAGGTAAAAGGCCAAACCTCAAA
GTCCACATCAATACCACAAGTGACTCCATCCTCTTGAAGTTCTTGCGTCCAAGTCCAAAT
GTAAAGCTTGAAGGTCTTCTCCTGGGATATGGCAGCAATGTATCACCAAACCAGTACTTC
CCTCTTCCCGCTGAAGGGAAATTCACAGAAGCTATAGTTGATGCAGAGCCGAAATATCTG
ATAGTTGTGCGACCTGCTCCACCTCCAAGTCAAAAGAAGTCATGTTCAGGTAAAACTCGT
TCTCGCAAACCTCTGCAGCTGGTGGTTGGCACTCTGACACCGAGCTCAGTCTTCCTGTCC
TGGGGTTTCCTCATCAACCCACACCATGACTGGACATTGCCAAGTCACTGTCCCAATGAC
AGATTTTATACAATTCGCTATCGAGAAAAGGATAAAGAAAAGAAGTGGATTTTTCAAATC
TGTCCAGCCACTGAAACAATTGTGGAAAACCTAAAGCCCAACACAGTTTATGAATTTGGA
GTGAAAGACAATGTGGAAGGTGGAATTTGGAGTAAGATTTTCAATCACAAGACTGTTGTT
GGAAGTAAAAAAGTAAATGGGAAAATCCAAAGTACCTATGACCAAGACCACACAGTGCCA
GCATATGTCCCAAGGAAACTAATCCCAATAACAATCATCAAGCAAGTGATTCAGAATGTT
ACTCACAAGGATTCAGCTAAATCCCCAGAAAAAGCTCCACTGGGAGGAGTGATACTAGTC
CACCTTATTATTCCAGGTCTTAATGAAACTACTGTAAAACTTCCTGCATCCCTAATGTTT
GAGATTTCAGATGCACTCAAGACACAATTAGCTAAGAATGAAACCTTGGCATTACCTGCC
GAATCTAAAACACCAGAGGTTGAAAAAATCTCAGCACGACCCACAACAGTGACTCCTGAA
ACAGTTCCAAGAAGCACTAAACCCACTACGTCTAGTGCATTAGATGTTTCAGAAACAACA
CTGGCTTCAAGTGAAAAGCCATGGATTGTGCCTACAGCTAAAATATCTGAAGATTCCAAA
GTTCTGCAGCCTCAAACTGCAACTTATGATGTTTTCTCAAGCCCTACAACATCAGATGAG
CCTGAGATATCAGATTCCTACACAGCAACAAGTGATCGTATTCTGGATTCTATCCCACCT
AAAACTTCTAGAACTCTTGAACAGCCAAGGGCAACACTGGCTCCAAGTGAAACACCATTT
GTTCCTCAAAAACTGGAAATCTTTACCAGTCCAGAAATGCAGCCTACGACACCTGCTCCC
CAGCAAACTACATCTATCCCTTCTACACCTAAACGACGCCCCCGGCCCAAACCGCCAAGA
ACCAAACCTGAAAGAACCACAAGTGCCGGAACAATTACACCTAAAATTTCTAAAAGCCCT
GAACCTACATGGACAACACCGGCTCCCGGTAAAACACAATTTATTTCTCTGAAACCTAAA
ATCCCTCTCAGCCCAGAAGTGACACACACCAAACCTGCTCCCAAGCAGACACCACGTGCT
CCTCCTAAGCCAAAAACATCACCACGCCCAAGAATCCCACAAACACAACCAGTTCCTAAG
GTGCCCCAGCGTGTTACTGCAAAACCAAAAACGTCACCAAGTCCAGAAGTGTCATACACC
ACACCTGCTCCAAAAGATGTGCTCCTTCCTCATAAACCATACCCTGAGGTCTCTCAGAGC
GAACCTGCTCCTCTAGAGACACGAGGCATCCCTTTTATACCCATGATTTCCCCAAGTCCT
AGTCAAGAGGAACTACAGACCACTCTGGAAGAAACAGACCAATCCACCCAAGAACCTTTC
ACAACTAAGATTCCACGAACAACTGAACTAGCAAAGACAACTCAGGCGCCACACAGATTT
TATACTACTGTGAGGCCCAGAACATCTGACAAGCCACACATCAGACCTGGGGTCAAGCAA
GCACCCAGGCCATCAGGTGCTGATAGAAATGTATCAGTGGACTCTACCCACCCCACTAAA
AAGCCAGGGACTCGCCGCCCACCCTTGCCACCCAGACCTACACACCCACGAAGAAAACCT
TTACCACCAAATAATGTCACTGGAAAGCCAGGAAGTGCAGGAATCATTTCATCAGGCCCA
ATAACTACACCACCCCTGAGGTCAACACCCAGGCCTACTGGAACTCCCTTGGAGAGAATA
GAGACAGATATAAAGCAACCAACAGTTCCTGCCTCTGGAGAAGAACTGGAAAATATAACT
GACTTTAGCTCAAGCCCAACAAGAGAAACTGATCCTCTTGGGAAGCCAAGATTCAAAGGA
CCTCATGTGCGATACATCCAAAAGCCTGACAACAGTCCCTGCTCCATTACTGACTCTGTC
AAACGGTTCCCCAAAGAGGAGGCCACAGAGGGGAATGCCACCAGCCCACCACAGAACCCA
CCCACCAACCTCACTGTGGTCACCGTGGAAGGGTGCCCCTCATTTGTCATCTTGGACTGG
GAAAAGCCACTAAATGACACTGTCACTGAATATGAAGTTATATCCAGAGAAAATGGGTCA
TTCAGTGGGAAGAACAAGTCCATTCAAATGACAAATCAGACATTTTCCACAGTAGAAAAT
CTGAAACCAAACACGAGTTATGAATTCCAGGTGAAACCCAAAAACCCGCTTGGTGAAGGC
CCGGTCAGCAACACAGTGGCATTCAGTACTGAATCAGCGGACCCAAGAGTGAGTGAGCCA
GTTTCTGCAGGAAGAGATGCCATCTGGACTGAAAGACCCTTTAATTCAGACTCTTACTCA
GAGTGTAAGGGCAAACAATATGTCAAAAGGACATGGTATAAAAAATTTGTAGGAGTGCAG
CTGTGCAACTCTCTCAGATACAAGATTTACTTGAGCGACTCCCTCACAGGAAAATTTTAT
AACATAGGTGATCAGAGGGGCCATGGAGAAGATCACTGCCAGTTTGTGGATTCATTTTTA
GATGGACGCACTGGGCAGCAACTCACTTCTGACCAGTTACCAATCAAAGAAGGTTATTTC
AGAGCAGTTCGCCAGGAACCTGTCCAATTTGGAGAAATAGGTGGTCACACCCAAATCAAT
TATGTTCAGTGGTATGAATGTGGGACTACAATTCCTGGAAAATGG
Protein sequence predicted based on nucleotide sequence
MRGGKCNMLSSLGCLLLCGSITLALGNAQKLPKGKRPNLKVHINTTSDSILLKFLRPSPN VKLEGLLLGYGSNVSPNQYFPLPAEGKFTEAIVDAEPKYLIVVRPAPPPSQKKSCSGKTR SRKPLQLVVGTLTPSSVFLSWGFLINPHHDWTLPSHCPNDRFYTIRYREKDKEKKWIFQI CPATETIVENLKPNTVYEFGVKDNVEGGIWSKIFNHKTVVGSKKVNGKIQSTYDQDHTVP AYVPRKLIPITIIKQVIQNVTHKDSAKSPEKAPLGGVILVHLIIPGLNETTVKLPASLMF EISDALKTQLAKNETLALPAESKTPEVEKISARPTTVTPETVPRSTKPTTSSALDVSETT LASSEKPWIVPTAKISEDSKVLQPQTATYDVFSSPTTSDEPEISDSYTATSDRILDSIPP KTSRTLEQPRATLAPSETPFVPQKLEIFTSPEMQPTTPAPQQTTSIPSTPKRRPRPKPPR TKPERTTSAGTITPKISKSPEPTWTTPAPGKTQFISLKPKIPLSPEVTHTKPAPKQTPRA PPKPKTSPRPRIPQTQPVPKVPQRVTAKPKTSPSPEVSYTTPAPKDVLLPHKPYPEVSQS EPAPLETRGIPFIPMISPSPSQEELQTTLEETDQSTQEPFTTKIPRTTELAKTTQAPHRF YTTVRPRTSDKPHIRPGVKQAPRPSGADRNVSVDSTHPTKKPGTRRPPLPPRPTHPRRKP LPPNNVTGKPGSAGIISSGPITTPPLRSTPRPTGTPLERIETDIKQPTVPASGEELENIT DFSSSPTRETDPLGKPRFKGPHVRYIQKPDNSPCSITDSVKRFPKEEATEGNATSPPQNP PTNLTVVTVEGCPSFVILDWEKPLNDTVTEYEVISRENGSFSGKNKSIQMTNQTFSTVEN LKPNTSYEFQVKPKNPLGEGPVSNTVAFSTESADPRVSEPVSAGRDAIWTERPFNSDSYS ECKGKQYVKRTWYKKFVGVQLCNSLRYKIYLSDSLTGKFYNIGDQRGHGEDHCQFVDSFL DGRTGQQLTSDQLPIKEGYFRAVRQEPVQFGEIGGHTQINYVQWYECGTTIPGKW
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3293
Start on reference sequence
66
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
