IGSF3 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  IGSF3
Gene Name:  immunoglobulin superfamily, member 3
Original Clone ID:
FLH483318.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296976
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3585nts
Open reading frame: 1 to 3585

ATGAAGTGCTTTTTCCCGGTGCTGAGCTGTCTGGCTGTGCTGGGTGTGGTGTCAGCACAG
CGGCAGGTCACCGTTCAGGAAGGACCCTTGTACCGCACGGAGGGCTCCCACATCACTATC
TGGTGCAATGTGAGTGGCTACCAGGGACCTTCTGAGCAGAATTTCCAGTGGTCCATTTAC
CTGCCTTCGTCGCCAGAGCGAGAGGTGCAGATCGTCAGCACCATGGACTCTTCCTTCCCC
TATGCCATCTACACCCAGCGCGTCCGCGGAGGGAAGATCTTCATAGAAAGAGTCCAGGGG
AACTCAACCCTATTGCACATCACAGATCTTCAGGCCCGGGATGCCGGGGAGTATGAATGC
CACACACCCAGCACTGATAAGCAATACTTTGGGAGTTACAGTGCAAAGATGAACCTAGTG
GTGATCCCAGACTCCCTGCAGACCACTGCCATGCCCCAGACTCTGCACAGAGTGGAGCAG
GACCCGCTGGAGCTCACTTGTGAGGTGGCCTCAGAGACCATTCAGCACAGCCACCTGTCT
GTGGCCTGGCTCCGGCAGAAAGTTGGCGAGAAGCCCGTGGAGGTCATCTCCCTGAGCCGA
GATTTCATGCTTCACTCCAGCAGCGAATATGCCCAGAGGCAGAGCCTGGGGGAGGTGCGG
CTGGACAAGCTGGGGAGGACCACCTTCCGCCTCACCATCTTCCACCTGCAGCCTTCTGAC
CAGGGCGAATTCTACTGCGAGGCCGCCGAGTGGATCCAGGATCCGGATGGGTCGTGGTAT
GCTATGACCCGAAAGCGTTCCGAGGGAGCCGTGGTCAACGTCCAGCCAACTGACAAAGAA
TTCACTGTTCGGCTGGAGACAGAGAAGCGGCTGCACACGGTGGGCGAGCCGGTGGAGTTC
AGATGCATCCTGGAGGCTCAGAATGTTCCCGACCGTTACTTTGCTGTCTCCTGGGCCTTC
AACAGCTCGCTCATCGCCACCATGGGTCCTAACGCTGTGCCTGTCCTCAACAGCGAATTT
GCTCACCGGGAAGCCAGGGGACAGCTTAAGGTGGCCAAAGAGAGCGACAGTGTCTTTGTG
CTGAAGATCTACCACCTCCGCCAGGAAGATAGCGGGAAATACAACTGCCGGGTGACTGAG
CGAGAGAAAACCGTGACCGGGGAATTCATTGATAAGGAGAGCAAGCGTCCCAAGAACATC
CCCATCATAGTCCTCCCCCTCAAGAGCAGCATCTCCGTGGAGGTGGCCAGCAATGCCAGC
GTCATCCTTGAGGGCGAGGACCTGCGCTTCTCCTGCAGTGTCCGCACGGCAGGCAGGCCG
CAGGGTCGCTTCTCTGTCATCTGGCAGCTTGTGGACAGGCAGAACCGCCGCAGCAATATC
ATGTGGCTAGACCGGGATGGCACCGTGCAGCCAGGCTCGTCCTACTGGGAGCGCAGCAGC
TTTGGGGGCGTCCAGATGGAGCAGGTGCAGCCCAACTCGTTCAGCCTGGGCATCTTCAAC
AGCAGGAAGGAGGACGAGGGCCAGTATGAATGCCATGTGACTGAATGGGTGCGGGCAGTG
GATGGCGAGTGGCAGATTGTTGGGGAGCGCCGGGCCAGCACTCCCATCTCCATCACAGCT
CTTGAAATGGGCTTCGCAGTCACAGCCATCTCCCGGACACCGGGGGTGACCTACAGCGAC
TCCTTTGACTTGCAGTGTATCATCAAACCCCACTACCCTGCCTGGGTCCCCGTGTCGGTG
ACATGGCGGTTCCAGCCGGTGGGCACGGTGGAGTTCCATGACTTGGTGACCTTCACCCGG
GACGGAGGGGTCCAGTGGGGGGACAGGTCCTCCAGCTTCCGAACCCGAACTGCCATCGAG
AAGGCTGAGTCCAGCAACAACGTCCGCCTAAGCATCAGCCGAGCCAGTGACACGGAAGCA
GGCAAGTACCAGTGTGTGGCAGAGCTGTGGCGGAAGAACTACAACAACACCTGGACGCGA
CTGGCGGAGAGGACCTCCAACCTGCTGGAGATCAGGGTGCTGCAGCCAGTGACAAAGCTG
CAGGTGAGCAAATCGAAGAGGACCCTCACCCTGGTGGAAAACAAGCCCATTCAGTTGAAC
TGCTCAGTCAAGTCTCAGACTAGCCAGAACTCCCACTTTGCGGTGCTCTGGTATGTCCAC
AAGCCCTCGGATGCCGATGGCAAGCTTATCCTGAAGACCACCCACAACTCCGCCTTTGAA
TACGGTACTTACGCCGAGGAGGAGGGCCTGAGAGCCAGGCTCCAGTTTGAGAGGCATGTG
TCGGGGGGCCTGTTCAGCCTCACCGTCCAGAGAGCCGAGGTCAGCGACAGCGGCAGCTAC
TACTGCCACGTGGAGGAGTGGCTGCTGAGCCCCAACTACGCCTGGTACAAGCTGGCAGAG
GAGGTTTCTGGGCGCACAGAAGTCACTGTGAAACAGCCAGACAGCCGCCTGAGGCTCAGC
CAAGCCCAGGGGAACCTGTCGGTTCTGGAGACCCGGCAGGTACAGCTGGAGTGTGTGGTT
CTCAACCGCACCAGCATAACCTCCCAGCTCATGGTGGAATGGTTTGTATGGAAGCCCAAC
CACCCTGAGCGGGAGACTGTGGCCCGCTTGAGCCGTGACGCCACCTTCCACTATGGAGAG
CAGGCAGCCAAGAACAATCTGAAGGGGCGGCTGCATTTGGAGAGTCCTTCCCCCGGCGTG
TACCGTCTCTTCATCCAGAACGTGGCTGTGCAGGACAGCGGGACCTACAGCTGCCATGTG
GAGGAGTGGCTGCCCAGCCCCAGTGGCATGTGGTATAAGCGGGCAGAGGACACCGCTGGG
CAGACAGCTCTGACAGTCATGCGACCAGATGCTTCCCTGCAGGTGGACACAGTGGTCCCC
AATGCCACGGTCTCTGAGAAGGCAGCTTTCCAGCTGGACTGTAGCATCGTGTCCCGCTCC
AGCCAGGACTCCCGCTTCGCTGTGGCCTGGTATTCCCTGAGGACTAAAGCTGGGGGGAAA
AGGAGCAGCCCTGGCCTGGAAGAACAGGAAGAGGAAAGGGAGGAGGAGGAGGAGGAGGAG
GAGGACGACGACGACGACGACCCAACAGAGCGGACGGCCCTGCTGAGCGTGGGCCCAGAT
GCTGTCTTTGGCCCAGAGGGCAGTCCTTGGGAGGGCAGGCTTCGCTTCCAGAGGCTCTCC
CCGGTGCTCTACCGGCTCACAGTGCTGCAGGCAAGCCCCCAAGATACAGGCAATTACTCC
TGCCATGTGGAGGAGTGGCTGCCCAGCCCTCAGAAGGAATGGTACCGGCTGACGGAGGAG
GAGTCAGCCCCCATCGGCATCCGTGTTCTAGATACAAGTCCCACCCTCCAGTCCATCATC
TGCTCCAACGACGCACTCTTCTACTTCGTCTTCTTCTACCCTTTCCCCATCTTTGGCATT
CTTATCATCACCATCCTTCTGGTGCGTTTCAAGAGCCGGAACTCCAGCAAGAACTCTGAT
GGGAAGAATGGGGTGCCTCTGCTGTGGATCAAAGAGCCACACCTCAACTACTCCCCTACT
TGCCTGGAGCCCCCTGTTCTCAGTATCCATCCAGGGGCCATAGAC

Protein sequence predicted based on nucleotide sequence

MKCFFPVLSCLAVLGVVSAQRQVTVQEGPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIY
LPSSPEREVQIVSTMDSSFPYAIYTQRVRGGKIFIERVQGNSTLLHITDLQARDAGEYEC
HTPSTDKQYFGSYSAKMNLVVIPDSLQTTAMPQTLHRVEQDPLELTCEVASETIQHSHLS
VAWLRQKVGEKPVEVISLSRDFMLHSSSEYAQRQSLGEVRLDKLGRTTFRLTIFHLQPSD
QGEFYCEAAEWIQDPDGSWYAMTRKRSEGAVVNVQPTDKEFTVRLETEKRLHTVGEPVEF
RCILEAQNVPDRYFAVSWAFNSSLIATMGPNAVPVLNSEFAHREARGQLKVAKESDSVFV
LKIYHLRQEDSGKYNCRVTEREKTVTGEFIDKESKRPKNIPIIVLPLKSSISVEVASNAS
VILEGEDLRFSCSVRTAGRPQGRFSVIWQLVDRQNRRSNIMWLDRDGTVQPGSSYWERSS
FGGVQMEQVQPNSFSLGIFNSRKEDEGQYECHVTEWVRAVDGEWQIVGERRASTPISITA
LEMGFAVTAISRTPGVTYSDSFDLQCIIKPHYPAWVPVSVTWRFQPVGTVEFHDLVTFTR
DGGVQWGDRSSSFRTRTAIEKAESSNNVRLSISRASDTEAGKYQCVAELWRKNYNNTWTR
LAERTSNLLEIRVLQPVTKLQVSKSKRTLTLVENKPIQLNCSVKSQTSQNSHFAVLWYVH
KPSDADGKLILKTTHNSAFEYGTYAEEEGLRARLQFERHVSGGLFSLTVQRAEVSDSGSY
YCHVEEWLLSPNYAWYKLAEEVSGRTEVTVKQPDSRLRLSQAQGNLSVLETRQVQLECVV
LNRTSITSQLMVEWFVWKPNHPERETVARLSRDATFHYGEQAAKNNLKGRLHLESPSPGV
YRLFIQNVAVQDSGTYSCHVEEWLPSPSGMWYKRAEDTAGQTALTVMRPDASLQVDTVVP
NATVSEKAAFQLDCSIVSRSSQDSRFAVAWYSLRTKAGGKRSSPGLEEQEEEREEEEEEE
EDDDDDDPTERTALLSVGPDAVFGPEGSPWEGRLRFQRLSPVLYRLTVLQASPQDTGNYS
CHVEEWLPSPQKEWYRLTEEESAPIGIRVLDTSPTLQSIICSNDALFYFVFFYPFPIFGI
LIITILLVRFKSRNSSKNSDGKNGVPLLWIKEPHLNYSPTCLEPPVLSIHPGAID

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 4356
Start on reference sequence 769
Mutations:
None
Discrepancy:
a1873g,S625G; t3566c,I1189T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable