DLC1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DLC1
Gene Name:  deleted in liver cancer 1
Original Clone ID:
FLH483321.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00296979
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 4584nts
Open reading frame: 1 to 4584

ATGTCTGTAGCTATCAGAAAGAGAAGCTGGGAAGAACATGTGACCCACTGGATGGGACAG
CCTTTTAATTCTGATGATCGTAACACAGCATGTCATCATGGACTAGTAGCTGACAGCTTG
CAGGCAAGTATGGAAAAAGATGCAACTCTAAATGTGGACCGCAAAGAGAAGTGTGTTTCA
CTACCTGACTGCTGTCATGGATCAGAGCTGAGAGATTTTCCTGGGAGGCCAATGGGTCAT
CTTTCAAAGGATGTGGACGAAAATGACAGCCATGAAGGTGAAGATCAGTTTCTTTCTCTG
GAAGCCAGCACAGAAACACTAGTGCATGTTTCTGATGAGGATAACAATGCTGATTTATGC
CTTACAGATGATAAACAGGTTTTAAATACCCAAGGGCAGAAAACATCAGGCCAACATATG
ATCCAAGGAGCAGGCTCCTTAGAAAAGGCACTGCCCATCATACAAAGTAACCAAGTTTCT
TCTAACTCCTGGGGAATAGCTGGTGAAACTGAATTAGCACTGGTAAAAGAAAGTGGGGAG
AGAAAAGTTACTGACTCTATAAGTAAAAGCCTGGAGCTTTGCAATGAAATAAGCTTAAGT
GAAATAAAAGATGCACCCAAAGTAAATGCAGTGGATACTTTGAACGTGAAAGATATTGCA
CCTGAGAAACAATTGCTTAACTCTGCTGTAATTGCTCAGCAACGAAGGAAACCTGACCCC
CCTAAAGATGAAAATGAAAGAAGCACCTGCAATGTAGTACAAGATGAGTTCTTGGATACT
CCTTGCACAAACAGAGGACTGCCATTATTAAAAACAGATTTTGGAAGCTGCCTTCTGCAG
CCTCCTTCCTGCCCCAATGGAATGTCAGCTGAAAATGGCCTGGAGAAGAGTGGTTTTTCA
CAACATCAAAACAAAAGTCCACCAAAGGTCAAGGCAGAAGATGGCATGCAGTGTTTACAA
TTAAAGGAGACCCTGGCCACCCAGGAACCCACAGATAACCAAGTCAGACTTCGTAAGAGA
AAGGAAATAAGAGAAGATCGAGATAGGGCGCGGCTGGACTCCATGGTGCTGCTGATTATG
AAACTGGACCAGCTTGATCAGGACATAGAAAATGCCCTCAGCACCAGCTCCTCTCCATCA
GGCACACCAACAAACCTGCGGCGGCACGTTCCTGATCTGGAATCAGGATCTGAAAGTGGA
GCAGATACCATTTCAGTAAATCAGACACGAGTAAATTTGTCTTCTGACACTGAGTCCACG
GACCTCCCATCTTCCACTCCAGTAGCCAATTCTGGAACCAAACCCAAGACTACGGCTATT
CAAGGTATTTCAGAGAAGGAAAAGGCTGAAATTGAAGCCAAGGAAGCTTGTGATTGGCTA
CGGGCAACTGGTTTCCCCCAGTATGCACAGCTTTATGAAGATTTCCTGTTCCCCATCGAT
ATTTCCTTGGTCAAGAGAGAGCATGATTTTTTGGACAGAGATGCCATTGAGGCTCTATGC
AGGCGTCTAAATACTTTAAACAAATGTGCGGTGATGAAGCTAGAAATTAGTCCTCATCGG
AAACGAAGTGACGATTCAGACGAGGATGAGCCTTGTGCCATCAGTGGCAAATGGACTTTC
CAAAGGGACAGCAAGAGGTGGTCCCGGCTTGAAGAGTTTGATGTCTTTTCTCCAAAACAA
GACCTGGTCCCTGGGTCCCCAGACGACTCCCACCCGAAGGACGGCCCCAGCCCCGGAGGC
ACGCTGATGGACCTCAGCGAGCGCCAGGAGGTGTCTTCCGTCCGCAGCCTCAGCAGCACT
GGCAGCCTCCCCAGCCACGCTCCCCCCAGCGAGGATGCTGCCACCCCCCGGACTAACTCC
GTCATCAGCGTTTGCTCCTCCAGCAACTTGGCAGGCAATGACGACTCTTTCGGCAGCCTG
CCCTCTCCCAAGGAACTGTCCAGCTTCAGCTTCAGCATGAAAGGCCACGAAAAAACTGCC
AAGTCCAAGACGCGCAGTCTGCTGAAACGGATGGAGAGCCTGAAGCTCAAGAGCTCCCAT
CACAGCAAGCACAAAGCGCCCTCAAAGCTGGGGTTGATCATCAGCGGGCCCATCTTGCAA
GAGGGGATGGATGAGGAGAAGCTGAAGCAGCTCAACTGCGTGGAGATCTCCGCCCTCAAT
GGCAACCGCATCAACGTCCCCATGGTACGAAAGAGGAGCGTTTCCAACTCCACGCAGACC
AGCAGCAGCAGCAGCCAGTCGGAGACCAGCAGCGCGGTCAGCACGCCCAGCCCTGTTACG
AGGACCCGGAGCCTCAGTGCGTGCAACAAGCGGGTGGGCATGTACTTAGAGGGCTTCGAT
CCTTTCAATCAGTCAACATTTAACAACGTGGTGGAGCAGAACTTTAAGAACCGCGAGAGC
TACCCGGAGGACACGGTGTTCTACATCCCTGAAGATCACAAGCCTGGCACTTTCCCCAAA
GCTCTCACCAATGGCAGTTTCTCCCCCTCGGGGAATAACGGCTCTGTGAACTGGAGGACG
GGAAGCTTCCACGGCCCTGGCCACATCAGCCTCAGGAGGGAAAACAGTAGCGACAGCCCC
AAGGAACTGAAGAGACGCAATTCTTCCAGCTCCATGAGCAGCCGCCTGAGCATCTACGAC
AACGTGCCGGGCTCCATCCTCTACTCCAGTTCAGGGGACCTGGCGGATCTGGAGAACGAG
GACATCTTCCCCGAGCTGGACGACATCCTCTACCACGTGAAGGGGATGCAGCGGATAGTC
AATCAGTGGTCGGAGAAGTTTTCTGATGAGGGAGATTCGGACTCAGCCCTGGACTCGGTC
TCTCCCTGCCCGTCCTCTCCAAAACAGATACACCTGGATGTGGACAACGACCGAACCACA
CCCAGCGACCTGGACAGCACAGGCAACTCCCTGAATGAACCGGAAGAGCCCTCCGAGATC
CCGGAAAGAAGGGATTCTGGGGTTGGGGCTTCCCTAACCAGGTCCAACAGGCACCGACTG
AGATGGCACAGTTTCCAGAGCTCACATCGGCCAAGCCTCAACTCTGTATCACTACAGATT
AACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCACTCCTAAAGCTAACG
GCCCTGCTGGAGAAATACACACCTTCTAACAAGCATGGTTTTAGCTGGGCCGTGCCCAAG
TTCATGAAGAGGATCAAGGTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTG
ACGGTCAACGTGCAGCGCACAGGACAACCGTTGCCTCAGAGCATCCAGCAGGCCATGCGA
TACCTCCGGAACCATTGTTTGGATCAGGTTGGGCTCTTCAGAAAATCGGGGGTCAAGTCC
CGGATTCAGGCTCTGCGCCAGATGAATGAAGGTGCCATAGACTGTGTCAACTACGAAGGA
CAGTCTGCTTATGACGTGGCAGACATGCTGAAGCAGTATTTTCGAGATCTTCCTGAGCCA
CTAATGACGAACAAACTCTCGGAAACCTTTCTACAGATCTACCAATATGTGCCCAAGGAC
CAGCGCCTGCAGGCCATCAAGGCTGCCATCATGCTGCTGCCTGACGAGAACCGGGAGGTT
CTGCAGACCCTGCTTTATTTCCTGAGCGATGTCACAGCAGCCGTAAAAGAAAACCAGATG
ACCCCAACCAACCTGGCCGTGTGCTTAGCGCCTTCCCTCTTCCATCTCAACACCCTGAAG
AGAGAGAATTCCTCTCCCAGGGTAATGCAAAGAAAACAAAGTTTGGGCAAACCAGATCAG
AAAGATTTGAATGAAAACCTAGCTGCCACTCAAGGGCTGGCCCATATGATCGCCGAGTGC
AAGAAGCTTTTCCAGGTTCCCGAGGAAATGAGCCGATGTCGTAATTCCTATACCGAACAA
GAGCTGAAGCCCCTCACTCTGGAAGCACTCGGGCACCTGGGTAATGATGACTCAGCTGAC
TACCAACACTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAAGAAGTCAAAGAGAAGTTT
AAAGGCTGGGTCAGCTACTCCACTTCGGAGCAGGCTGAGCTGTCCTATAAGAAGGTGAGC
GAAGGACCCCCTCTGAGGCTTTGGAGGTCAGTCATTGAAGTCCCTGCTGTGCCAGAGGAA
ATCTTAAAGCGCCTACTTAAAGAACAGCACCTCTGGGATGTAGACCTGTTGGATTCAAAA
GTGATCGAAATTCTGGACAGCCAAACTGAAATTTACCAGTATGTCCAAAACAGTATGGCA
CCTCATCCTGCTCGAGACTACGTTGTTTTAAGAACCTGGAGGACTAATTTACCCAAAGGA
GCCTGTGCCCTTTTACTAACCTCTGTGGATCACGATCGCGCACCTGTGGTGGGTGTGAGG
GTTAATGTGCTCTTGTCCAGGTATTTGATTGAACCCTGTGGGCCAGGAAAATCCAAACTC
ACCTACATGTGCAGAGTTGACTTAAGGGGCCACATGCCAGAATGGTACACAAAATCTTTT
GGACATTTGTGTGCAGCTGAAGTTGTAAAGATCCGGGATTCCTTCAGTAACCAGAACACT
GAAACCAAAGACACCAAATCTAGG

Protein sequence predicted based on nucleotide sequence

MSVAIRKRSWEEHVTHWMGQPFNSDDRNTACHHGLVADSLQASMEKDATLNVDRKEKCVS
LPDCCHGSELRDFPGRPMGHLSKDVDENDSHEGEDQFLSLEASTETLVHVSDEDNNADLC
LTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGE
RKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKDIAPEKQLLNSAVIAQQRRKPDP
PKDENERSTCNVVQDEFLDTPCTNRGLPLLKTDFGSCLLQPPSCPNGMSAENGLEKSGFS
QHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIM
KLDQLDQDIENALSTSSSPSGTPTNLRRHVPDLESGSESGADTISVNQTRVNLSSDTEST
DLPSSTPVANSGTKPKTTAIQGISEKEKAEIEAKEACDWLRATGFPQYAQLYEDFLFPID
ISLVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSDDSDEDEPCAISGKWTF
QRDSKRWSRLEEFDVFSPKQDLVPGSPDDSHPKDGPSPGGTLMDLSERQEVSSVRSLSST
GSLPSHAPPSEDAATPRTNSVISVCSSSNLAGNDDSFGSLPSPKELSSFSFSMKGHEKTA
KSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEISALN
GNRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFD
PFNQSTFNNVVEQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRT
GSFHGPGHISLRRENSSDSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENE
DIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTT
PSDLDSTGNSLNEPEEPSEIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQI
NCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPL
TVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEG
QSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREV
LQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQ
KDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSAD
YQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEE
ILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKG
ACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPEWYTKSF
GHLCAAEVVKIRDSFSNQNTETKDTKSR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 200
End on reference sequence 4786
Mutations:
None
Discrepancy:
a3193g,I1065V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable