Gene:
Gene Symbol:
ADAMTS17
Gene Name:
ADAM metallopeptidase with thrombospondin type 1 motif, 17
Original Clone ID:
FLH269715.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297043
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTGTGACGGCGCCCTGCTGCCTCCGCTCGTCCTGCCCGTGCTGCTGCTGCTGGTTTGG
GGACTGGACCCGGGCACAGCTGTCGGCGACGCGGCGGCCGACGTGGAGGTGGTGCTCCCG
TGGCGGGTGCGCCCCGACGACGTGCACCTGCCGCCGCTGCCCGCAGCCCCCGGGCCCCGA
CGGCGGCGACGCCCCCGCACGCCCCCAGCCGCCCCGCGCGCCCGGCCCGGAGAGCGCGCC
CTGCTGCTGCACCTGCCGGCCTTCGGGCGCGACCTGTACCTTCAGCTGCGCCGCGACCTG
CGCTTCCTGTCCCGAGGCTTCGAGGTGGAGGAGGCGGGCGCGGCCCGGCGCCGCGGCCGC
CCCGCCGAGCTGTGCTTCTACTCGGGCCGTGTGCTCGGCCACCCCGGCTCCCTCGTCTCG
CTCAGCGCCTGCGGCGCCGCCGGCGGCCTGGTTGGCCTCATTCAGCTTGGGCAGGAGCAG
GTGCTAATCCAGCCCCTCAACAACTCCCAGGGCCCATTCAGTGGACGAGAACATCTGATC
AGGCGCAAATGGTCCTTGACCCCCAGCCCTTCTGCTGAGGCCCAGAGACCTGAGCAGCTC
TGCAAGGTTCTAACAGAAAAGAAGAAGCCGACGTGGGGCAGGCCTTCGCGGGACTGGCGG
GAGCGGAGGAACGCTATCCGGCTCACCAGCGAGCACACGGTGGAGACCCTGGTGGTGGCC
GACGCCGACATGGTGCAGTACCACGGGGCCGAGGCCGCCCAGAGGTTCATCCTGACCGTC
ATGAACATGGTATACAATATGTTTCAGCACCAGAGCCTGGGGATTAAAATTAACATTCAA
GTGACCAAGCTTGTCCTGCTACGACAACGTCCCGCTAAGTTGTCCATTGGGCACCATGGT
GAGCGGTCCCTGGAGAGCTTCTGTCACTGGCAGAACGAGGAGTATGGAGGAGCGCGATAC
CTCGGCAATAACCAGGTTCCCGGCGGGAAGGACGACCCGCCCCTGGTGGATGCTGCCGTG
TTTGTGACCAGGACAGATTTCTGTGTACACAAGGATGAACCGTGTGACACTGTTGGAATT
GCTTACTTAGGAGGTGTGTGCAGTGCTAAGAGGAAGTGTGTGCTTGCCGAAGACAATGGT
CTCAATTTGGCCTTTACCATCGCCCATGAGCTGGGCCACAACTTGGGCATGAACCACGAC
GATGACCACTCATCTTGCGCTGGCAGGTCCCACATCATGTCAGGAGAGTGGGTGAAAGGC
CGGAACCCAAGTGACCTCTCTTGGTCCTCCTGCAGCCGAGATGACCTTGAAAACTTCCTC
AAGTCAAAAGTCAGCACCTGCTTGCTAGTCACGGACCCCAGAAGCCAGCACACAGTACGC
CTCCCGCACAAGCTGCCGGGCATGCACTACAGTGCCAACGAGCAGTGCCAGATCCTGTTT
GGCATGAATGCCACCTTCTGCAGAAACATGGAGCATCTAATGTGTGCTGGACTGTGGTGC
CTGGTAGAAGGAGACACATCCTGCAAGACCAAGCTGGACCCTCCCCTGGATGGCACCGAG
TGTGGGGCAGACAAGTGGTGCCGCGCGGGGGAGTGCGTGAGCAAGACGCCCATCCCGGAG
CATGTGGACGGAGACTGGAGCCCGTGGGGCGCCTGGAGCATGTGCAGCCGAACATGTGGG
ACGGGAGCCCGCTTCAGGCAGAGGAAATGTGACAACCCCCCCCCTGGGCCTGGAGGCACA
CACTGCCCGGGTGCCAGTGTAGAACATGCGGTCTGCGAGAACCTGCCCTGCCCCAAGGGT
CTGCCCAGCTTCCGGGACCAGCAGTGCCAGGCACACGACCGGCTGAGCCCCAAGAAGAAA
GGCCTGCTGACAGCCGTGGTGGTTGACGATAAGCCATGTGAACTCTACTGCTCGCCCCTC
GGGAAGGAGTCCCCACTGCTGGTGGCCGACAGGGTCCTGGACGGTACACCCTGCGGGCCC
TACGAGACTGATCTCTGCGTGCACGGCAAGTGCCAGAAAATCGGCTGTGACGGCATCATC
GGGTCTGCAGCCAAAGAGGACAGATGCGGGGTCTGCAGCGGGGACGGCAAGACCTGCCAC
TTGGTGAAGGGCGACTTCAGCCACGCCCGGGGGACAGCTCTCAAAGACTCGGGTAAGGGG
TCCATCAACAGTGACTGGAAGATAGAGCTCCCCGGAGAGTTCCAGATTGCAGGCACAACT
GTTCGCTATGTGAGAAGGGGGCTGTGGGAGAAGATCTCTGCCAAGGGACCAACCAAACTA
CCGCTGCACTTGATGGTGTTGTTATTTCACGACCAAGATTATGGAATTCATTATGAATAC
ACTGTTCCTGTAAACCGCACTGCGGAAAATCAAAGCGAACCAGAAAAACCGCAGGACTCT
TTGTTCATCTGGACCCACAGCGGCTGGGAAGGGTGCAGTGTGCAGTGCGGCGGAGGGGAG
CGCAGAACCATCGTCTCGTGTACACGGATTGTCAACAAGACCACAACTCTGGTGAACGAC
AGTGACTGCCCTCAAGCAAGCCGCCCAGAGCCCCAGGTCCGAAGGTGCAACTTGCACCCC
TGCCAGTCACGGTGGGTGGCAGGCCCGTGGAGCCCCTGCTCGGCGACCTGTGAGAAAGGC
TTCCAGCACCGGGAGGTGACCTGCGTGTACCAGCTGCAGAACGGCACACACGTCGCTACG
CGGCCCCTCTACTGCCCGGGCCCCCGGCCGGCGGCAGTGCAGAGCTGTGAAGGCCAGGAC
TGCCTGTCCATCTGGGAGGCGTCTGAGTGGTCACAGTGCTCTGCCAGCTGTGGTAAAGGG
GTGTGGAAACGGACCGTGGCGTGCACCAACTCACAAGGGAAATGCGACGCATCCACGAGG
CCGAGAGCCGAGGAGGCCTGCGAGGACTACTCAGGCTGCTACGAGTGGAAAACTGGGGAC
TGGTCTACGTGCTCGTCGACCTGCGGGAAGGGCCTGCAGTCCCGGGTGGTGCAGTGCATG
CACAAGGTCACAGGGCGCCACGGCAGCGAGTGCCCCGCCCTCTCGAAGCCTGCCCCCTAC
AGACAGTGCTACCAGGAGGTCTGCAACGACAGGATCAACGCCAACACCATCACCTCCCCC
CGCCTTGCTGCTCTGACCTACAAATGCACACGAGACCAGTGGACGGTATATTGCCGGGTC
ATCCGAGAAAAGAACCTCTGCCAGGACATGCGGTGGTACCAGCGCTGCTGCCAGACCTGC
AGGGACTTCTATGCAAACAAGATGCGCCAGCCACCGCCGAACTCG
Protein sequence predicted based on nucleotide sequence
MCDGALLPPLVLPVLLLLVWGLDPGTAVGDAAADVEVVLPWRVRPDDVHLPPLPAAPGPR RRRRPRTPPAAPRARPGERALLLHLPAFGRDLYLQLRRDLRFLSRGFEVEEAGAARRRGR PAELCFYSGRVLGHPGSLVSLSACGAAGGLVGLIQLGQEQVLIQPLNNSQGPFSGREHLI RRKWSLTPSPSAEAQRPEQLCKVLTEKKKPTWGRPSRDWRERRNAIRLTSEHTVETLVVA DADMVQYHGAEAAQRFILTVMNMVYNMFQHQSLGIKINIQVTKLVLLRQRPAKLSIGHHG ERSLESFCHWQNEEYGGARYLGNNQVPGGKDDPPLVDAAVFVTRTDFCVHKDEPCDTVGI AYLGGVCSAKRKCVLAEDNGLNLAFTIAHELGHNLGMNHDDDHSSCAGRSHIMSGEWVKG RNPSDLSWSSCSRDDLENFLKSKVSTCLLVTDPRSQHTVRLPHKLPGMHYSANEQCQILF GMNATFCRNMEHLMCAGLWCLVEGDTSCKTKLDPPLDGTECGADKWCRAGECVSKTPIPE HVDGDWSPWGAWSMCSRTCGTGARFRQRKCDNPPPGPGGTHCPGASVEHAVCENLPCPKG LPSFRDQQCQAHDRLSPKKKGLLTAVVVDDKPCELYCSPLGKESPLLVADRVLDGTPCGP YETDLCVHGKCQKIGCDGIIGSAAKEDRCGVCSGDGKTCHLVKGDFSHARGTALKDSGKG SINSDWKIELPGEFQIAGTTVRYVRRGLWEKISAKGPTKLPLHLMVLLFHDQDYGIHYEY TVPVNRTAENQSEPEKPQDSLFIWTHSGWEGCSVQCGGGERRTIVSCTRIVNKTTTLVND SDCPQASRPEPQVRRCNLHPCQSRWVAGPWSPCSATCEKGFQHREVTCVYQLQNGTHVAT RPLYCPGPRPAAVQSCEGQDCLSIWEASEWSQCSASCGKGVWKRTVACTNSQGKCDASTR PRAEEACEDYSGCYEWKTGDWSTCSSTCGKGLQSRVVQCMHKVTGRHGSECPALSKPAPY RQCYQEVCNDRINANTITSPRLAALTYKCTRDQWTVYCRVIREKNLCQDMRWYQRCCQTC RDFYANKMRQPPPNS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3367
Start on reference sequence
80
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
