DNASU Plasmid | MMP20 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: MMP20

Gene Name: matrix metallopeptidase 20

Original Clone ID:

FLH269754.01L

Keyword:

STOP codon removed based on OC annotation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00297082

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1449nts

Open reading frame: 1 to 1449

Reference Sequence Alignment

ATGAAGGTGCTCCCTGCATCTGGCCTTGCTGTCTTCCTCATCATGGCTTTGAAGTTTTCC
ACTGCAGCCCCCTCCCTAGTTGCAGCCTCCCCCAGGACCTGGAGGAACAACTACCGCCTC
GCACAGGCGTATCTTGACAAATATTACACAAATAAAGAAGGACACCAGATTGGTGAGATG
GTTGCAAGAGGAAGCAATTCCATGATAAGGAAGATTAAGGAGCTACAAGCGTTCTTTGGC
CTCCAAGTCACCGGGAAGTTAGACCAGACCACAATGAACGTGATCAAGAAGCCTCGCTGT
GGAGTTCCTGATGTGGCCAATTATCGCCTCTTCCCTGGTGAACCCAAATGGAAAAAAAAT
ACTTTGACATACAGAATATCTAAATACACACCTTCCATGAGTTCTGTCGAGGTGGACAAA
GCAGTGGAGATGGCCTTGCAGGCCTGGAGTAGCGCCGTCCCTCTGAGCTTTGTCAGAATA
AACTCAGGAGAAGCGGATATTATGATATCTTTTGAAAATGGAGATCACGGGGATTCCTAT
CCATTCGATGGGCCTCGGGGGACTCTAGCCCATGCATTTGCTCCTGGAGAAGGCCTGGGA
GGAGATACACATTTCGACAATGCTGAGAAGTGGACTATGGGAACGAATGGTTTTAATTTG
TTTACCGTTGCTGCTCATGAATTTGGCCATGCCCTGGGCCTGGCCCATTCCACAGACCCA
TCAGCACTGATGTACCCAACTTATAAGTACAAGAATCCCTATGGATTCCACCTCCCCAAA
GATGATGTGAAAGGGATCCAGGCATTATACGGACCTCGGAAAGTATTCCTGGGGAAGCCC
ACTCTGCCCCATGCCCCCCATCACAAGCCATCCATCCCTGACCTCTGTGACTCCAGCTCA
TCCTTTGACGCTGTGACAATGCTGGGGAAGGAGCTCCTGCTCTTCAAGGACCGGATTTTC
TGGAGACGGCAGGTTCACTTGCGGACAGGAATTCGGCCCAGCACTATTACCAGCTCCTTC
CCCCAGCTCATGTCCAATGTGGATGCAGCTTACGAAGTGGCTGAGAGGGGCACTGCTTAC
TTCTTCAAAGGTCCCCACTACTGGATAACAAGAGGATTCCAAATGCAAGGTCCTCCTCGG
ACTATTTATGACTTTGGATTTCCAAGGCACGTGCAGCAAATAGATGCTGCTGTCTACCTC
AGGGAGCCACAGAAGACCCTTTTCTTTGTGGGAGATGAATACTACAGCTACGACGAAAGG
AAAAGGAAAATGGAAAAAGACTATCCAAAGAATACTGAAGAAGAATTTTCAGGAGTAAAT
GGCCAAATCGATGCTGCTGTAGAATTAAATGGCTACATTTACTTCTTTTCAGGACCAAAA
ACATACAAGTATGACACAGAGAAGGAAGATGTGGTTAGTGTGGTGAAATCTAGTTCCTGG
ATTGGTTGC

Protein sequence predicted based on nucleotide sequence

MKVLPASGLAVFLIMALKFSTAAPSLVAASPRTWRNNYRLAQAYLDKYYTNKEGHQIGEM
VARGSNSMIRKIKELQAFFGLQVTGKLDQTTMNVIKKPRCGVPDVANYRLFPGEPKWKKN
TLTYRISKYTPSMSSVEVDKAVEMALQAWSSAVPLSFVRINSGEADIMISFENGDHGDSY
PFDGPRGTLAHAFAPGEGLGGDTHFDNAEKWTMGTNGFNLFTVAAHEFGHALGLAHSTDP
SALMYPTYKYKNPYGFHLPKDDVKGIQALYGPRKVFLGKPTLPHAPHHKPSIPDLCDSSS
SFDAVTMLGKELLLFKDRIFWRRQVHLRTGIRPSTITSSFPQLMSNVDAAYEVAERGTAY
FFKGPHYWITRGFQMQGPPRTIYDFGFPRHVQQIDAAVYLREPQKTLFFVGDEYYSYDER
KRKMEKDYPKNTEEEFSGVNGQIDAAVELNGYIYFFSGPKTYKYDTEKEDVVSVVKSSSW
IGC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 14

End on reference sequence 1465

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

MMP20

Symbol Nomenclature :

MMP20

SYNONYM :

AI2A2; MMP-20

Designation :

enamel metalloproteinase|matrix metalloproteinase 20 (enamelysin)|matrix metalloproteinase-20

Full Nomenclature :

matrix metallopeptidase 20

GENEID :

9313

GI :

157170227

GenBank Accession :

BC152741

HGNC :

7167

MIM :

604629

Vega :

OTTHUMG00000168081

Target GenBank:

NM_004771.3

Reference Sequence Alignment

  

HsCD00297082      GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAAGGTGCTCCCTGCATCTGGCCT
NM_004771.3       ----------------------------------ATGAAGGTGCTCCCTGCATCTGGCCT
                                                    **************************

HsCD00297082      TGCTGTCTTCCTCATCATGGCTTTGAAGTTTTCCACTGCAGCCCCCTCCCTAGTTGCAGC
NM_004771.3       TGCTGTCTTCCTCATCATGGCTTTGAAGTTTTCCACTGCAGCCCCCTCCCTAGTTGCAGC
                  ************************************************************

HsCD00297082      CTCCCCCAGGACCTGGAGGAACAACTACCGCCTCGCACAGGCGTATCTTGACAAATATTA
NM_004771.3       CTCCCCCAGGACCTGGAGGAACAACTACCGCCTCGCACAGGCGTATCTTGACAAATATTA
                  ************************************************************

HsCD00297082      CACAAATAAAGAAGGACACCAGATTGGTGAGATGGTTGCAAGAGGAAGCAATTCCATGAT
NM_004771.3       CACAAATAAAGAAGGACACCAGATTGGTGAGATGGTTGCAAGAGGAAGCAATTCCATGAT
                  ************************************************************

HsCD00297082      AAGGAAGATTAAGGAGCTACAAGCGTTCTTTGGCCTCCAAGTCACCGGGAAGTTAGACCA
NM_004771.3       AAGGAAGATTAAGGAGCTACAAGCGTTCTTTGGCCTCCAAGTCACCGGGAAGTTAGACCA
                  ************************************************************

HsCD00297082      GACCACAATGAACGTGATCAAGAAGCCTCGCTGTGGAGTTCCTGATGTGGCCAATTATCG
NM_004771.3       GACCACAATGAACGTGATCAAGAAGCCTCGCTGTGGAGTTCCTGATGTGGCCAATTATCG
                  ************************************************************

HsCD00297082      CCTCTTCCCTGGTGAACCCAAATGGAAAAAAAATACTTTGACATACAGAATATCTAAATA
NM_004771.3       CCTCTTCCCTGGTGAACCCAAATGGAAAAAAAATACTTTGACATACAGAATATCTAAATA
                  ************************************************************

HsCD00297082      CACACCTTCCATGAGTTCTGTCGAGGTGGACAAAGCAGTGGAGATGGCCTTGCAGGCCTG
NM_004771.3       CACACCTTCCATGAGTTCTGTCGAGGTGGACAAAGCAGTGGAGATGGCCTTGCAGGCCTG
                  ************************************************************

HsCD00297082      GAGTAGCGCCGTCCCTCTGAGCTTTGTCAGAATAAACTCAGGAGAAGCGGATATTATGAT
NM_004771.3       GAGTAGCGCCGTCCCTCTGAGCTTTGTCAGAATAAACTCAGGAGAAGCGGATATTATGAT
                  ************************************************************

HsCD00297082      ATCTTTTGAAAATGGAGATCACGGGGATTCCTATCCATTCGATGGGCCTCGGGGGACTCT
NM_004771.3       ATCTTTTGAAAATGGAGATCACGGGGATTCCTATCCATTCGATGGGCCTCGGGGGACTCT
                  ************************************************************

HsCD00297082      AGCCCATGCATTTGCTCCTGGAGAAGGCCTGGGAGGAGATACACATTTCGACAATGCTGA
NM_004771.3       AGCCCATGCATTTGCTCCTGGAGAAGGCCTGGGAGGAGATACACATTTCGACAATGCTGA
                  ************************************************************

HsCD00297082      GAAGTGGACTATGGGAACGAATGGTTTTAATTTGTTTACCGTTGCTGCTCATGAATTTGG
NM_004771.3       GAAGTGGACTATGGGAACGAATGGTTTTAATTTGTTTACCGTTGCTGCTCATGAATTTGG
                  ************************************************************

HsCD00297082      CCATGCCCTGGGCCTGGCCCATTCCACAGACCCATCAGCACTGATGTACCCAACTTATAA
NM_004771.3       CCATGCCCTGGGCCTGGCCCATTCCACAGACCCATCAGCACTGATGTACCCAACTTATAA
                  ************************************************************

HsCD00297082      GTACAAGAATCCCTATGGATTCCACCTCCCCAAAGATGATGTGAAAGGGATCCAGGCATT
NM_004771.3       GTACAAGAATCCCTATGGATTCCACCTCCCCAAAGATGATGTGAAAGGGATCCAGGCATT
                  ************************************************************

HsCD00297082      ATACGGACCTCGGAAAGTATTCCTGGGGAAGCCCACTCTGCCCCATGCCCCCCATCACAA
NM_004771.3       ATACGGACCTCGGAAAGTATTCCTGGGGAAGCCCACTCTGCCCCATGCCCCCCATCACAA
                  ************************************************************

HsCD00297082      GCCATCCATCCCTGACCTCTGTGACTCCAGCTCATCCTTTGACGCTGTGACAATGCTGGG
NM_004771.3       GCCATCCATCCCTGACCTCTGTGACTCCAGCTCATCCTTTGACGCTGTGACAATGCTGGG
                  ************************************************************

HsCD00297082      GAAGGAGCTCCTGCTCTTCAAGGACCGGATTTTCTGGAGACGGCAGGTTCACTTGCGGAC
NM_004771.3       GAAGGAGCTCCTGCTCTTCAAGGACCGGATTTTCTGGAGACGGCAGGTTCACTTGCGGAC
                  ************************************************************

HsCD00297082      AGGAATTCGGCCCAGCACTATTACCAGCTCCTTCCCCCAGCTCATGTCCAATGTGGATGC
NM_004771.3       AGGAATTCGGCCCAGCACTATTACCAGCTCCTTCCCCCAGCTCATGTCCAATGTGGATGC
                  ************************************************************

HsCD00297082      AGCTTACGAAGTGGCTGAGAGGGGCACTGCTTACTTCTTCAAAGGTCCCCACTACTGGAT
NM_004771.3       AGCTTACGAAGTGGCTGAGAGGGGCACTGCTTACTTCTTCAAAGGTCCCCACTACTGGAT
                  ************************************************************

HsCD00297082      AACAAGAGGATTCCAAATGCAAGGTCCTCCTCGGACTATTTATGACTTTGGATTTCCAAG
NM_004771.3       AACAAGAGGATTCCAAATGCAAGGTCCTCCTCGGACTATTTATGACTTTGGATTTCCAAG
                  ************************************************************

HsCD00297082      GCACGTGCAGCAAATAGATGCTGCTGTCTACCTCAGGGAGCCACAGAAGACCCTTTTCTT
NM_004771.3       GCACGTGCAGCAAATAGATGCTGCTGTCTACCTCAGGGAGCCACAGAAGACCCTTTTCTT
                  ************************************************************

HsCD00297082      TGTGGGAGATGAATACTACAGCTACGACGAAAGGAAAAGGAAAATGGAAAAAGACTATCC
NM_004771.3       TGTGGGAGATGAATACTACAGCTACGACGAAAGGAAAAGGAAAATGGAAAAAGACTATCC
                  ************************************************************

HsCD00297082      AAAGAATACTGAAGAAGAATTTTCAGGAGTAAATGGCCAAATCGATGCTGCTGTAGAATT
NM_004771.3       AAAGAATACTGAAGAAGAATTTTCAGGAGTAAATGGCCAAATCGATGCTGCTGTAGAATT
                  ************************************************************

HsCD00297082      AAATGGCTACATTTACTTCTTTTCAGGACCAAAAACATACAAGTATGACACAGAGAAGGA
NM_004771.3       AAATGGCTACATTTACTTCTTTTCAGGACCAAAAACATACAAGTATGACACAGAGAAGGA
                  ************************************************************

HsCD00297082      AGATGTGGTTAGTGTGGTGAAATCTAGTTCCTGGATTGGTTGCTCAGGCCTCATGGGCCC
NM_004771.3       AGATGTGGTTAGTGTGGTGAAATCTAGTTCCTGGATTGGTTGCTAA--------------
                  ********************************************.*              

HsCD00297082      AGCTTTCTTGTAC
NM_004771.3       -------------