Gene:
Gene Symbol:
FANCD2
Gene Name:
Fanconi anemia, complementation group D2
Original Clone ID:
FLH269771.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297099
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGTTTCCAAAAGAAGACTGTCAAAATCTGAGGATAAAGAGAGCCTGACAGAAGATGCC
TCCAAAACCAGGAAGCAACCACTTTCCAAAAAGACAAAGAAATCTCATATTGCTAATGAA
GTTGAAGAAAATGACAGCATCTTTGTAAAGCTTCTTAAGATATCAGGAATTATTCTTAAA
ACGGGAGAGAGTCAGAATCAACTAGCTGTGGATCAAATAGCTTTCCAAAAGAAGCTCTTT
CAGACCCTGAGGAGACACCCTTCCTATCCCAAAATAATAGAAGAATTTGTTAGTGGCCTG
GAGTCTTACATTGAGGATGAAGACAGTTTCAGGAACTGCCTTTTGTCTTGTGAGCGTCTG
CAGGATGAGGAAGCCAGTATGGGTGCATCTTATTCTAAGAGTCTCATCAAACTGCTTCTG
GGGATTGACATACTGCAGCCTGCCATTATCAAAACCTTATTTGAGAAGTTGCCAGAATAT
TTTTTTGAAAACAAGAACAGTGATGAAATCAACATACCTCGACTCATTGTCAGTCAACTA
AAATGGCTTGACAGAGTTGTGGATGGCAAGGACCTCACCACCAAGATCATGCAGCTGATC
AGTATTGCTCCAGAGAACCTGCAGCATGACATCATCACCAGCCTACCTGAGATCCTAGGG
GATTCCCAGCACGCTGATGTGGGGAAAGAACTCAGTGACCTACTGATAGAGAATACTTCA
CTCACTGTCCCAATCCTGGATGTCCTTTCAAGCCTCCGACTTGACCCAAACTTCCTATTG
AAGGTTCGCCAGTTGGTGATGGATAAGTTGTCGTCTATTAGATTGGAGGATTTACCTGTG
ATAATAAAGTTCATTCTTCATTCCGTAACAGCCATGGATACACTTGAGGTAATTTCTGAG
CTTCGGGAGAAGTTGGATCTGCAGCATTGTGTTTTGCCATCACGGTTACAGGCTTCCCAA
GTAAAGTTGAAAAGTAAAGGACGAGCAAGTTCCTCAGGAAATCAAGAAAGCAGCGGTCAG
AGCTGTATTATTCTCCTCTTTGATGTAATAAAGTCAGCTATTAGATATGAGAAAACCATT
TCAGAAGCCTGGATTAAGGCAATTGAAAACACTGCCTCAGTATCTGAACACAAGGTGTTT
GACCTGGTGATGCTTTTCATCATCTATAGCACCAATACTCAGACAAAGAAGTACATTGAC
AGGGTGCTAAGAAATAAGATTCGATCAGGCTGCATTCAAGAACAGCTGCTCCAGAGTACA
TTCTCTGTTCATTACTTAGTTCTTAAGGATATGTGTTCATCCATTCTGTCGCTGGCTCAG
AGTTTGCTTCACTCTCTAGACCAGAGTATAATTTCATTTGGCAGTCTCCTATACAAATAT
GCATTTAAGTTTTTTGACACGTACTGCCAGCAGGAAGTGGTTGGTGCCTTAGTGACCCAT
ATCTGCAGTGGGAATGAAGCTGAAGTTGATACTGCCTTAGATGTCCTTCTAGAGTTGGTA
GTGTTAAACCCATCTGCTATGATGATGAATGCTGTCTTTGTAAAGGGCATTTTAGATTAT
CTGGATAACATATCCCCTCAGCAAATACGAAAACTCTTCTATGTTCTCAGCACACTGGCA
TTTAGCAAACAGAATGAAGCCAGCAGCCACATCCAGGATGACATGCACTTGGTGATAAGA
AAGCAGCTCTCTAGCACCGTATTCAAGTACAAGCTCATTGGGATTATTGGTGCTGTGACC
ATGGCTGGCATCATGGCGGCAGACAGAAGTGAATCACCTAGTTTGACCCAAGAGAGAGCC
AACCTGAGCGATGAGCAGTGCACACAGGTGACCTCCTTGTTGCAGTTGGTTCATTCCTGC
AGTGAGCAGTCTCCTCAGGCCTCTGCACTTTACTATGATGAATTTGCCAACCTGATCCAA
CATGAAAAGCTGGATCCAAAAGCCCTGGAATGGGTTGGGCATACCATCTGTAATGATTTC
CAGGATGCCTTCGTAGTGGACTCCTGTGTTGTTCCGGAAGGTGACTTTCCATTTCCTGTG
AAAGCACTGTACGGACTGGAAGAATACGACACTCAGGATGGGATTGCCATAAACCTCCTG
CCGCTGCTGTTTTCTCAGGACTTTGCAAAAGATGGGGGTCCGGTGACCTCACAGGAATCA
GGCCAAAAATTGGTGTCTCCGCTGTGCCTGGCTCCGTATTTCCGGTTACTGAGACTTTGT
GTGGAGAGACAGCATAACGGAAACTTGGAGGAGATTGATGGTCTACTAGATTGTCCTATA
TTCCTAACTGACCTGGAGCCTGGAGAGAAGTTGGAGTCCATGTCTGCTAAAGAGCGTTCA
TTCATGTGTTCTCTCATATTTCTTACTCTCAACTGGTTCCGAGAGATTGTAAATGCCTTC
TGCCAGGAAACATCACCTGAGATGAAGGGGAAGGTGCTCACTCGGTTAAAGCACATTGTA
GAATTGCAAATAATCCTGGAAAAGTACTTGGCAGTCACCCCAGACTATGTCCCTCCTCTT
GGAAACTTTGATGTGGAAACTTTAGATATAACACCTCATACTGTTACTGCTATTTCAGCA
AAAATCAGAAAGAAAGGAAAAATAGAAAGGAAACAAAAAACAGATGGCAGCAAGACATCC
TCCTCTGACACACTTTCAGAAGAGAAAAATTCAGAATGTGACCCTACGCCATCTCATAGA
GGCCAGCTAAACAAGGAGTTCACAGGGAAGGAAGAAAAGACATCATTGTTACTACATAAT
TCCCATGCTTTTTTCCGAGAGCTGGACATTGAGGTCTTCTCTATTCTACATTGTGGACTT
GTGACGAAGTTCATCTTAGATACTGAAATGCACACTGAAGCTACAGAAGTTGTGCAACTT
GGGCCCCCTGAGCTGCTTTTCTTGCTGGAAGATCTCTCCCAGAAGCTGGAGAGTATGCTG
ACACCTCCTATTGCCAGGAGAGTCCCCTTTCTCAAGAACAAAGGAAGCCGGAATATTGGA
TTCTCACATCTCCAACAGAGATCTGCCCAAGAAATTGTTCATTGTGTTTTTCAACTGCTG
ACCCCAATGTGTAACCACCTGGAGAACATTCACAACTATTTTCAGTGTTTAGCTGCTGAG
AATCACGGTGTAGTTGATGGACCAGGAGTGAAAGTTCAGGAGTACCACATAATGTCTTCC
TGCTATCAGAGGCTGCTGCAGATTTTTCATGGGCTTTTTGCTTGGAGTGGATTTTCTCAA
CCTGAAAATCAGAATTTACTGTATTCAGCCCTCCATGTCCTTAGTAGCCGACTGAAACAG
GGAGAACACAGCCAGCCTTTGGAGGAACTACTCAGCCAGAGCGTCCATTACTTGCAGAAT
TTCCATCAAAGCATTCCCAGTTTCCAGTGTGCTCTTTATCTCATCAGACTTTTGATGGTT
ATTTTGGAGAAATCAACAGCTTCTGCTCAGAACAAAGAAAAAATTGCTTCCCTTGCCAGA
CAATTCCTCTGTCGGGTGTGGCCAAGTGGGGATAAAGAGAAGAGCAACATCTCTAATGAC
CAGCTCCATGCTCTGCTCTGTATCTACCTGGAGCACACAGAGAGCATTCTGAAGGCCATA
GAGGAGATTGCTGGTGTTGGTGTCCCAGAACTGATCAACTCTCCTAAAGATGCATCTTCC
TCCACATTCCCTACACTGACCAGGCATACTTTTGTTGTTTTCTTCCGTGTGATGATGGCT
GAACTAGAGAAGACGGTGAAAAAAATTGAGCCTGGCACAGCAGCAGACTCGCAGCAGATT
CATGAAGAGAAACTCCTCTACTGGAACATGGCTGTTCGAGACTTCAGTATCCTCATCAAC
TTGATAAAGGTATTTGATAGTCATCCTGTTCTGCATGTATGTTTGAAGTATGGGCGTCTC
TTTGTGGAAGCATTTCTGAAGCAATGTATGCCGCTCCTAGACTTCAGTTTTAGAAAACAC
CGGGAAGATGTTCTGAGCTTACTGGAAACCTTCCAGTTGGACACAAGGCTGCTTCATCAC
CTGTGTGGGCATTCCAAGATTCACCAGGACACGAGACTCACCCAACATGTGCCTCTGCTC
AAAAAGACCCTGGAACTTTTAGTTTGCAGAGTCAAAGCTATGCTCACTCTCAACAATTGT
AGAGAGGCTTTCTGGCTGGGCAATCTAAAAAACCGGGACTTGCAGGGTGAAGAGATTAAG
TCCCAAAATTCCCAGGAGAGCACAGCAGATGAGAGTGAGGATGACATGTCATCCCAGGCC
TCCAAGAGCAAAGCCACTGAGGTATCTCTACAAAACCCACCAGAGTCTGGCACTGATGGT
TGCATTTTGTTAATTGTTCTAAGTTGGTGGAGCAGAACTTTGCCTACTTATGTTTATTGT
CAAATGCTTCTATGCCCATTTCCATTCCCTCCA
Protein sequence predicted based on nucleotide sequence
MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILK TGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERL QDEEASMGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQL KWLDRVVDGKDLTTKIMQLISIAPENLQHDIITSLPEILGDSQHADVGKELSDLLIENTS LTVPILDVLSSLRLDPNFLLKVRQLVMDKLSSIRLEDLPVIIKFILHSVTAMDTLEVISE LREKLDLQHCVLPSRLQASQVKLKSKGRASSSGNQESSGQSCIILLFDVIKSAIRYEKTI SEAWIKAIENTASVSEHKVFDLVMLFIIYSTNTQTKKYIDRVLRNKIRSGCIQEQLLQST FSVHYLVLKDMCSSILSLAQSLLHSLDQSIISFGSLLYKYAFKFFDTYCQQEVVGALVTH ICSGNEAEVDTALDVLLELVVLNPSAMMMNAVFVKGILDYLDNISPQQIRKLFYVLSTLA FSKQNEASSHIQDDMHLVIRKQLSSTVFKYKLIGIIGAVTMAGIMAADRSESPSLTQERA NLSDEQCTQVTSLLQLVHSCSEQSPQASALYYDEFANLIQHEKLDPKALEWVGHTICNDF QDAFVVDSCVVPEGDFPFPVKALYGLEEYDTQDGIAINLLPLLFSQDFAKDGGPVTSQES GQKLVSPLCLAPYFRLLRLCVERQHNGNLEEIDGLLDCPIFLTDLEPGEKLESMSAKERS FMCSLIFLTLNWFREIVNAFCQETSPEMKGKVLTRLKHIVELQIILEKYLAVTPDYVPPL GNFDVETLDITPHTVTAISAKIRKKGKIERKQKTDGSKTSSSDTLSEEKNSECDPTPSHR GQLNKEFTGKEEKTSLLLHNSHAFFRELDIEVFSILHCGLVTKFILDTEMHTEATEVVQL GPPELLFLLEDLSQKLESMLTPPIARRVPFLKNKGSRNIGFSHLQQRSAQEIVHCVFQLL TPMCNHLENIHNYFQCLAAENHGVVDGPGVKVQEYHIMSSCYQRLLQIFHGLFAWSGFSQ PENQNLLYSALHVLSSRLKQGEHSQPLEELLSQSVHYLQNFHQSIPSFQCALYLIRLLMV ILEKSTASAQNKEKIASLARQFLCRVWPSGDKEKSNISNDQLHALLCIYLEHTESILKAI EEIAGVGVPELINSPKDASSSTFPTLTRHTFVVFFRVMMAELEKTVKKIEPGTAADSQQI HEEKLLYWNMAVRDFSILINLIKVFDSHPVLHVCLKYGRLFVEAFLKQCMPLLDFSFRKH REDVLSLLETFQLDTRLLHHLCGHSKIHQDTRLTQHVPLLKKTLELLVCRVKAMLTLNNC REAFWLGNLKNRDLQGEEIKSQNSQESTADESEDDMSSQASKSKATEVSLQNPPESGTDG CILLIVLSWWSRTLPTYVYCQMLLCPFPFPP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4494
Start on reference sequence
79
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
