Gene:
Gene Symbol:
KIF20B
Gene Name:
kinesin family member 20B
Original Clone ID:
FLH269772.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297100
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAATCTAATTTTAATCAAGAGGGAGTACCTCGACCATCTTATGTTTTTAGTGCTGAC
CCAATTGCAAGGCCTTCAGAAATAAATTTCGATGGCATTAAGCTTGATCTGTCTCATGAA
TTTTCCTTAGTTGCTCCAAATACTGAGGCAAACAGTTTCGAATCTAAAGATTATCTCCAG
GTTTGTCTTCGAATAAGACCATTTACACAGTCAGAAAAAGAACTTGAGTCTGAGGGCTGT
GTGCATATTCTGGATTCACAGACTGTTGTGCTGAAAGAGCCTCAATGCATCCTTGGTCGG
TTAAGTGAAAAAAGCTCAGGGCAGATGGCACAGAAATTCAGTTTTTCCAAGGTTTTTGGC
CCAGCAACTACACAGAAGGAATTCTTTCAGGGTTGCATTATGCAACCAGTAAAAGACCTC
TTGAAAGGACAGAGTCGTCTGATTTTTACTTACGGGCTAACCAATTCAGGAAAAACATAT
ACATTTCAAGGGACAGAAGAAAATATTGGCATTCTGCCTCGAACTTTGAATGTATTATTT
GATAGTCTTCAAGAAAGACTGTATACAAAGATGAACCTTAAACCACATAGATCCAGAGAA
TACTTAAGGTTATCATCAGAACAAGAGAAAGAAGAAATTGCTAGCAAAAGTGCATTGCTT
CGGCAAATTAAAGAGGTTACTGTGCATAATGATAGTGATGATACTCTTTATGGAAGTTTA
ACTAACTCTTTGAATATCTCAGAGTTTGAAGAATCCATAAAAGATTATGAACAAGCCAAC
TTGAATATGGCTAATAGTATAAAATTTTCTGTGTGGGTTTCTTTCTTTGAAATTTACAAT
GAATATATTTATGACTTATTTGTTCCTGTATCATCTAAATTCCAAAAGAGAAAGATGCTG
CGCCTTTCCCAAGACGTAAAGGGCTATTCTTTTATAAAAGATCTACAATGGATTCAAGTA
TCTGATTCCAAAGAAGCCTATAGACTTTTAAAACTAGGAATAAAGCACCAGAGTGTTGCC
TTCACAAAATTGAATAATGCTTCCAGTAGAAGTCACAGCATATTCACTGTTAAAATATTA
CAGATTGAAGATTCTGAAATGTCTCGTGTAATTCGAGTCAGTGAATTATCTTTATGTGAT
CTTGCTGGTTCAGAACGAACTATGAAGACACAGAATGAAGGTGAAAGGTTAAGAGAGACT
GGGAATATCAACACTTCTTTATTGACTCTGGGAAAGTGTATTAACGTCTTGAAGAATAGT
GAAAAGTCAAAGTTTCAACAGCATGTGCCTTTCCGGGAAAGTAAACTGACTCACTATTTT
CAAAGTTTTTTTAATGGTAAAGGGAAAATTTGTATGATTGTCAATATCAGCCAATGTTAT
TTAGCCTATGATGAAACACTCAATGTATTGAAGTTCTCCGCCATTGCACAAAAAGTTTGT
GTCCCAGACACTTTAAATTCCTCTCAAGAGAAATTATTTGGACCTGTCAAATCTTCTCAA
GATGTATCACTAGACAGTAATTCAAACAGTAAAATATTAAATGTAAAAAGAGCCACCATT
TCATGGGAAAATAGTCTAGAAGATTTGATGGAAGACGAGGATTTGGTTGAGGAGCTAGAA
AACGCTGAAGAAACTCAAAATGTGGAAACTAAACTTCTTGATGAAGATCTAGATAAAACA
TTAGAGGAAAATAAGGCTTTCATTAGCCACGAGGAGAAAAGAAAACTGTTGGACTTAATA
GAAGACTTGAAAAAAAAACTGATAAATGAAAAAAAGGAAAAATTAACCTTGGAATTTAAA
ATTCGAGAAGAAGTTACACAGGAGTTTACTCAGTATTGGGCTCAACGGGAAGCTGACTTT
AAGGAGACTCTGCTTCAAGAACGAGAGATATTAGAAGAAAATGCTGAACGTCGTTTGGCT
ATCTTCAAGGATTTGGTTGGTAAATGTGACACTCGAGAAGAAGCAGCGAAAGACATTTGT
GCCACAAAAGTTGAAACTGAAGAAGCTACTGCTTGTTTAGAACTAAAGTTTAATCAAATT
AAAGCTGAATTAGCTAAAACCAAAGGAGAATTAATCAAAACCAAAGAAGAGTTAAAAAAG
AGAGAAAATGAATCAGATTCATTGATTCAAGAGCTTGAGACATCTAATAAGAAAATAATT
ACACAGAATCAAAGAATTAAAGAATTGATAAATATAATTGATCAAAAAGAAGATACTATC
AACGAATTTCAGAACCTAAAGTCTCATATGGAAAACACATTTAAATGCAATGACAAGGCT
GATACATCTTCTTTAATAATAAACAATAAATTGATTTGTAATGAAACAGTTGAAGTACCT
AAGGACAGCAAATCTAAAATCTGTTCAGAAAGAAAAAGAGTAAATGAAAATGAACTTCAG
CAAGATGAACCACCAGCAAAGAAAGGGTCTATCCATGTTAGTTCAGCTATCACTGAAGAC
CAAAAGAAAAGTGAAGAAGTGCGACCGAACATTGCAGAAATTGAAGACATCAGAGTTTTA
CAAGAAAATAATGAAGGACTGAGAGCATTTTTACTCACTATTGAGAATGAACTTAAAAAT
GAAAAGGAAGAAAAAGCAGAATTAAATAAACAGATTGTTCATTTTCAGCAGGAACTTTCT
CTTTCTGAAAAAAAGAATTTAACTTTAAGTAAAGAGGTCCAACAAATTCAGTCAAATTAT
GATATTGCAATTGCTGAATTACATGTGCAGAAAAGTAAAAATCAAGAACAGGAGGAAAAG
ATCATGAAATTGTCAAATGAGATAGAAACTGCTACAAGAAGCATTACAAATAATGTTTCA
CAAATAAAATTAATGCACACGAAAATAGACGAACTACGTACTCTTGATTCAGTTTCTCAG
ATTTCAAACATAGATTTGCTCAATCTCAGGGATCTGTCAAATGGTTCTGAGGAGGATAAT
TTGCCAAATACACAGTTAGACCTTTTAGGTAATGATTATTTGGTAAGTAAGCAAGTTAAA
GAATATCGAATTCAAGAACCCAATAGGGAAAATTCTTTCCACTCTAGTATTGAAGCTATT
TGGGAAGAATGTAAAGAGATTGTGAAGGCCTCTTCCAAAAAAAGTCATCAGATTGAGGAA
CTGGAACAACAAATTGAAAAATTGCAGGCAGAAGTAAAAGGCTATAAGGATGAAAACAAT
AGACTAAAGGAGAAGGAGCATAAAAACCAAGATGACCTACTAAAAGAAAAAGAAACTCTT
ATACAGCAGCTGAAAGAAGAATTGCAAGAAAAAAATGTTACTCTTGATGTTCAAATACAG
CATGTAGTTGAAGGAAAGAGAGCGCTTTCAGAACTTACACAAGGTGTTACTTGCTATAAG
GCAAAAATAAAGGAACTTGAAACAATTTTAGAGACTCAGAAAGTTGAATGTAGTCATTCA
GCCAAGTTAGAACAAGACATTTTGGAAAAGGAATCTATCATCTTAAAGCTAGAAAGAAAT
TTGAAGGAATTTCAAGAACATCTTCAGGATTCTGTCAAAAACACCAAAGATTTAAATGTA
AAGGAACTCAAGCTGAAAGAAGAAATCACACAGTTAACAAATAATTTGCAAGATATGAAA
CATTTACTTCAATTAAAAGAAGAAGAAGAAGAAACCAACAGGCAAGAAACAGAAAAATTG
AAAGAGGAACTCTCTGCAAGCTCTGCTCGTACCCAGAATCTGAAAGCAGATCTTCAGAGG
AAGGAAGAAGATTATGCTGACCTGAAAGAGAAACTGACTGATGCCAAAAAGCAGATTAAG
CAAGTACAGAAAGAGGTATCTGTAATGCGTGATGAGGATAAATTACTGAGGATTAAAATT
AATGAACTGGAGAAAAAGAAAAACCAGTGTTCTCAGGAATTAGATATGAAACAGCGAACC
ATTCAGCAACTCAAGGAGCAGTTAAATAATCAGAAAGTGGAAGAAGCTATACAACAGTAT
GAGAGAGCATGCAAAGATCTAAATGTTAAAGAGAAAATAATTGAAGACATGCGAATGACA
CTAGAAGAACAGGAACAAACTCAGGTAGAACAGGATCAAGTGCTTGAGGCTAAATTAGAG
GAAGTTGAAAGGCTGGCCACAGAATTGGAAAAATGGAAGGAAAAATGCAATGATTTGGAA
ACCAAAAACAATCAAAGGTCAAATAAAGAACATGAGAACAACACAGATGTGCTTGGAAAG
CTCACTAATCTTCAAGATGAGTTACAGGAGTCTGAACAGAAATATAATGCTGATAGAAAG
AAATGGTTAGAAGAAAAAATGATGCTTATCACTCAAGCGAAAGAAGCAGAGAATATACGA
AATAAAGAGATGAAAAAATATGCTGAGGACAGGGAGCGTTTTTTTAAGCAACAGAATGAA
ATGGAAATACTGACAGCCCAGCTGACAGAGAAAGATAGTGACCTTCAAAAGTGGCGAGAA
GAACGAGATCAACTGGTTGCAGCTTTAGAAATACAGCTAAAAGCACTGATATCCAGTAAT
GTACAGAAAGATAATGAAATTGAACAACTAAAAAGGATCATATCAGAGACTTCTAAAATA
GAAACACAAATCATGGATATCAAGCCCAAACGTATTAGTTCAGCAGATCCTGACAAACTT
CAAACTGAACCTCTATCGACAAGTTTTGAAATTTCCAGAAATAAAATAGAGGATGGATCT
GTAGTCCTTGACTCTTGTGAAGTGTCAACAGAAAATGATCAAAGCACTCGATTTCCAAAA
CCTGAGTTAGAGATTCAATTTACACCTTTACAGCCAAACAAAATGGCAGTGAAACACCCT
GGTTGTACCACACCAGTGACAGTTAAGATTCCCAAGGCTCGGAAGAGGAAGAGTAATGAA
ATGGAGGAGGACTTGGTGAAATGTGAAAATAAGAAGAATGCTACACCCAGAACTAATTTG
AAATTTCCTATTTCAGATGATAGAAATTCTTCTGTCAAAAAGGAACAAAAGGTTGCCATA
CGTCCATCATCTAAGAAAACATATTCTTTACGGAGTCAGGCATCCATAATTGGTGTAAAC
CTGGCCACTAAGAAAAAAGAAGGAACACTACAGAAATTTGGAGACTTCTTACAACATTCT
CCCTCAATTCTTCAATCAAAAGCAAAGAAGATAATTGAAACAATGAGCTCTTCAAAGCTC
TCAAATGTAGAAGCAAGTAAAGAAAATGTGTCTCAACCAAAACGAGCCAAACGGAAATTA
TACACAAGTGAAATTTCATCTCCTATTGATATATCAGGCCAAGTGATTTTAATGGACCAG
AAAATGAAGGAGAGTGATCACCAGATTATCAAACGACGACTTCGAACAAAAACAGCCAAA
Protein sequence predicted based on nucleotide sequence
MESNFNQEGVPRPSYVFSADPIARPSEINFDGIKLDLSHEFSLVAPNTEANSFESKDYLQ VCLRIRPFTQSEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKVFG PATTQKEFFQGCIMQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLF DSLQERLYTKMNLKPHRSREYLRLSSEQEKEEIASKSALLRQIKEVTVHNDSDDTLYGSL TNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYNEYIYDLFVPVSSKFQKRKML RLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSRSHSIFTVKIL QIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSLLTLGKCINVLKNS EKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVC VPDTLNSSQEKLFGPVKSSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELE NAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFK IREEVTQEFTQYWAQREADFKETLLQEREILEENAERRLAIFKDLVGKCDTREEAAKDIC ATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRENESDSLIQELETSNKKII TQNQRIKELINIIDQKEDTINEFQNLKSHMENTFKCNDKADTSSLIINNKLICNETVEVP KDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIRVL QENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEKKNLTLSKEVQQIQSNY DIAIAELHVQKSKNQEQEEKIMKLSNEIETATRSITNNVSQIKLMHTKIDELRTLDSVSQ ISNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRENSFHSSIEAI WEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETL IQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVECSHS AKLEQDILEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMK HLLQLKEEEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIK QVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQY ERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLE TKNNQRSNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIR NKEMKKYAEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSN VQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGS VVLDSCEVSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRKSNE MEEDLVKCENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQASIIGVN LATKKKEGTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKL YTSEISSPIDISGQVILMDQKMKESDHQIIKRRLRTKTAK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
5415
Start on reference sequence
73
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
