Gene:
Gene Symbol:
SPAG1
Gene Name:
sperm associated antigen 1
Original Clone ID:
FLH269787.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297115
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACCACCAAAGATTATCCATCATTGTGGGGCTTTGGAACAACAAAAACATTCAAAATT
CCCATTGAACATCTAGATTTCAAATACATTGAAAAATGTTCAGATGTTAAACATCTGGAA
AAAATTCTTTGCGTGCTCAGATCTGGTGAGGAAGGATATTATCCTGAACTTACAGAATTT
TGTGAAAAGCATCTTCAAGCCTTGGCCCCTGAAAGCAGAGCTTTGAGGAAAGATAAACCA
GCAGCAACAGCAGCCAGTTTTACAGCTGAAGAATGGGAAAAAATTGATGGTGATATAAAG
AGTTGGGTATCAGAAATTAAAAAAGAAGAAGATAAAATGCACTTTCATGAAACTGAGACA
TTTCCAGCAATGAAAGATAATTTGCCTCCAGTTCGTGGTTCAAACAGCTGTCTTCATGTA
GGCAAGGAAAAATATTCTAAAAGACCAACTAAAAAGAAAACTCCAAGGGATTACGCGGAA
TGGGATAAATTTGACGTGGAGAAGGAATGTTTAAAAATTGATGAAGATTACAAAGAAAAG
ACGGTAATAGACAAGTCACACTTGTCTAAAATTGAGACAAGAATAGATACAGCAGGTCTA
ACTGAGAAAGAAAAGGATTTTCTTGCCACTCGTGAAAAGGAGAAAGGAAATGAAGCTTTC
AACTCAGGAGATTATGAAGAAGCAGTGATGTATTATACCAGGAGCATATCAGCGCTTCCC
ACTGTAGTTGCCTATAACAATCGAGCTCAAGCAGAAATCAAATTACAGAACTGGAATAGT
GCTTTTCAGGATTGTGAAAAGGTCTTGGAGTTAGAACCTGGAAACGTAAAGGCTCTTCTG
CGTCGTGCTACTACATATAAACATCAAAACAAGCTCCGGGAAGCTACAGAAGATTTGAGT
AAAGTACTAGATGTTGAGCCTGATAATGATTTGGCCAAGAAAACCTTGTCAGAGGTTGAA
AGAGATCTGAAAAATTCTGAAGCTGCATCTGAGACTCAAACCAAAGGGAAAAGGATGGTT
ATTCAGGAAATAGAAAACTCCGAAGATGAAGAAGGAAAAAGCGGAAGAAAACATGAAGAT
GGCGGTGGAGATAAGAAGCCCGCGGAGCCGGCGGGAGCCGCGCGCGCCGCCCAGCCGTGC
GTCATGGGCAACATCCAGAAGAAGCTGACTGGCAAAGCCGAAGGCGGCAAGCGGCCGGCA
AGGGGCGCGCCGCAGCGGGGCCAGACCCCGGAGGCCGGCGCGGACAAGCGGAGCCCACGG
CGGGCCTCTGCGGCGGCGGCGGCGGGCGGCGGCGCCACCGGGCATCCGGGCGGCGGGCAG
GGCGCGGAGAACCCTGCCGGCCTGAAGAGCCAGGGCAACGAGCTGTTCCGAAGCGGGCAG
TTCGCCGAGGCGGCCGGCAAGTACTCGGCGGCAATCGCGCTCCTGGAGCCAGCAGGAAGT
GAAATTGCAGATGATCTAAGTATCTTATATTCAAATAGAGCAGCATGTTACCTAAAAGAA
GGAAACTGCAGTGGCTGCATTCAAGATTGTAACAGGGCTCTGGAACTTCATCCATTCTCT
ATGAAACCTCTTCTGAGGCGGGCGATGGCCTATGAAACTCTAGAGCAGTATGGGAAAGCT
TATGTGGATTATAAAACAGTGTTGCAGATAGACTGTGGACTCCAGCTAGCAAATGACAGT
GTTAACAGGCTATCAAGAATTTTAATGGAGCTGGATGGACCAAATTGGCGGGAGAAGCTG
TCACCTATTCCTGCTGTGCCTGCTTCTGTGCCACTGCAAGCTTGGCATCCGGCAAAAGAG
ATGATCTCAAAACAAGCAGGAGACTCCAGCAGCCATCGCCAGCAGGGCATCACAGATGAA
AAAACATTTAAAGCCCTTAAGGAAGAAGGAAATCAATGTGTAAATGACAAAAACTATAAA
GACGCCCTCAGTAAATACAGCGAATGCTTAAAGATTAACAATAAGGAATGTGCCATATAT
ACAAACAGAGCTCTCTGTTACTTGAAGCTGTGCCAGTTTGAAGAAGCAAAGCAGGACTGT
GATCAGGCACTTCAGCTAGCTGATGGGAACGTGAAAGCCTTCTATAGACGAGCTCTGGCT
CATAAAGGACTCAAGAATTATCAGAAAAGCTTAATTGATCTCAATAAAGTTATCCTACTA
GATCCAAGTATTATTGAGGCAAAGATGGAACTGGAAGAGGTAACTAGACTCCTTAATCTT
AAGGATAAGACAGCACCATTCAACAAAGAAAAGGAGAGAAGGAAAATTGAGATTCAAGAG
GTGAATGAAGGCAAGGAGGAGCCTGGAAGACCTGCAGGGGAGGTCTCCATGGGATGCCTT
GCTTCTGAGAAGGGAGGCAAAAGCAGCAGGTCACCAGAAGACCCTGAGAAACTTCCGATA
GCCAAGCCTAATAATGCCTATGAATTTGGTCAGATTATAAATGCTCTCAGTACCAGGAAG
GATAAAGAAGCCTGTGCACATCTTTTAGCCATCACTGCACCAAAAGATTTGCCGATGTTT
TTAAGTAACAAACTTGAAGGGGATACATTCCTTCTCCTCATTCAGTCTCTGAAAAATAAT
CTTATTGAAAAAGATCCCTCATTGGTGTATCAGCATCTTTTATACCTGAGTAAAGCAGAA
AGGTTTAAGATGATGTTGACACTAATTAGCAAGGGCCAAAAGGAGCTAATTGAACAGCTG
TTTGAGGACCTTTCGGACACACCAAACAACCATTTTACTTTAGAAGATATACAGGCCCTA
AAAAGGCAGTATGAGCTT
Protein sequence predicted based on nucleotide sequence
MTTKDYPSLWGFGTTKTFKIPIEHLDFKYIEKCSDVKHLEKILCVLRSGEEGYYPELTEF CEKHLQALAPESRALRKDKPAATAASFTAEEWEKIDGDIKSWVSEIKKEEDKMHFHETET FPAMKDNLPPVRGSNSCLHVGKEKYSKRPTKKKTPRDYAEWDKFDVEKECLKIDEDYKEK TVIDKSHLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFNSGDYEEAVMYYTRSISALP TVVAYNNRAQAEIKLQNWNSAFQDCEKVLELEPGNVKALLRRATTYKHQNKLREATEDLS KVLDVEPDNDLAKKTLSEVERDLKNSEAASETQTKGKRMVIQEIENSEDEEGKSGRKHED GGGDKKPAEPAGAARAAQPCVMGNIQKKLTGKAEGGKRPARGAPQRGQTPEAGADKRSPR RASAAAAAGGGATGHPGGGQGAENPAGLKSQGNELFRSGQFAEAAGKYSAAIALLEPAGS EIADDLSILYSNRAACYLKEGNCSGCIQDCNRALELHPFSMKPLLRRAMAYETLEQYGKA YVDYKTVLQIDCGLQLANDSVNRLSRILMELDGPNWREKLSPIPAVPASVPLQAWHPAKE MISKQAGDSSSHRQQGITDEKTFKALKEEGNQCVNDKNYKDALSKYSECLKINNKECAIY TNRALCYLKLCQFEEAKQDCDQALQLADGNVKAFYRRALAHKGLKNYQKSLIDLNKVILL DPSIIEAKMELEEVTRLLNLKDKTAPFNKEKERRKIEIQEVNEGKEEPGRPAGEVSMGCL ASEKGGKSSRSPEDPEKLPIAKPNNAYEFGQIINALSTRKDKEACAHLLAITAPKDLPMF LSNKLEGDTFLLLIQSLKNNLIEKDPSLVYQHLLYLSKAERFKMMLTLISKGQKELIEQL FEDLSDTPNNHFTLEDIQALKRQYEL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2867
Start on reference sequence
87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
