Gene:
Gene Symbol:
GPR125
Gene Name:
G protein-coupled receptor 125
Original Clone ID:
FLH269896.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297128
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGCCACCCGGACGCCGGCGGGGCCGCGCGCAGCCGCCGCTGTTGCTGCCGCTCTCG
CTGTTAGCGCTGCTCGCGCTGCTGGGAGGCGGCGGCGGCGGCGGCGCCGCGGCGCTGCCC
GCCGGCTGCAAGCACGATGGGCGGCCCCGAGGGGCTGGCAGGGCGGCGGGCGCCGCCGAG
GGCAAGGTGGTGTGCAGCAGCCTGGAACTCGCGCAGGTCCTGCCCCCAGATACTCTGCCC
AACCGCACGGTCACCCTGATTCTGAGTAACAATAAGATATCCGAGCTGAAGAATGGCTCA
TTTTCTGGGTTAAGTCTCCTTGAAAGATTGGACCTCCGAAACAATCTTATTAGTAGTATA
GATCCAGGTGCCTTCTGGGGACTGTCATCTCTAAAAAGATTGGATCTGACAAACAATCGA
ATAGGATGTCTGAATGCAGACATATTTCGAGGACTCACCAATCTGGTTCGGCTAAACCTT
TCGGGGAATTTGTTTTCTTCATTATCTCAAGGAACTTTTGATTATCTTGCGTCATTACGG
TCTTTGGAATTCCAGACTGAGTATCTTTTGTGTGACTGTAACATACTGTGGATGCATCGC
TGGGTAAAGGAGAAGAACATCACGGTACGGGATACCAGGTGTGTTTATCCTAAGTCACTG
CAGGCCCAACCAGTCACAGGCGTGAAGCAGGAGCTGTTGACATGCGACCCTCCGCTTGAA
TTGCCGTCTTTCTACATGACTCCATCTCATCGCCAAGTTGTGTTTGAAGGAGACAGCCTT
CCTTTCCAGTGCATGGCTTCATATATTGATCAGGACATGCAAGTGTTGTGGTATCAGGAT
GGGAGAATAGTTGAAACCGATGAATCGCAAGGTATTTTTGTTGAAAAGAACATGATTCAC
AACTGCTCCTTGATTGCAAGTGCCCTAACCATTTCTAATATTCAGGCTGGATCTACTGGA
AATTGGGGCTGTCATGTCCAGACCAAACGTGGGAATAATACGAGGACTGTGGATATTGTG
GTATTAGAGAGTTCTGCACAGTACTGTCCTCCAGAGAGGGTGGTAAACAACAAAGGTGAC
TTCAGATGGCCCAGAACATTGGCAGGCATTACTGCATATCTGCAGTGTACGCGGAACACC
CATGGCAGTGGGATATATCCCGGAAACCCACAGGATGAGAGAAAAGCTTGGCGCAGATGT
GATAGAGGTGGCTTTTGGGCAGATGATGATTATTCTCGCTGTCAGTATGCAAATGATGTC
ACTAGAGTTCTTTATATGTTTAATCAGATGCCCCTCAATCTTACCAATGCCGTGGCAACA
GCTCGACAGTTACTGGCTTACACTGTGGAAGCAGCCAACTTTTCTGACAAAATGGATGTT
ATATTTGTGGCAGAAATGATTGAAAAATTTGGAAGATTTACCAAGGAGGAAAAATCAAAA
GAGCTAGGTGACGTGATGGTTGACATTGCAAGTAACATCATGTTGGCTGATGAACGTGTC
CTGTGGCTGGCGCAGAGGGAAGCTAAAGCCTGCAGTAGGATTGTGCAGTGTCTTCAGCGC
ATTGCTACCTACCGGCTAGCCGGTGGAGCTCACGTTTATTCAACATATTCACCCAATATT
GCTCTGGAAGCTTATGTCATCAAGTCTACTGGCTTCACGGGGATGACCTGTACCGTGTTC
CAGAAAGTGGCAGCCTCTGATCGTACAGGACTTTCGGATTATGGGAGGCGGGATCCAGAG
GGAAACCTGGATAAGCAGCTGAGCTTTAAGTGCAATGTTTCAAATACATTTTCGAGTCTG
GCACTAAAGAATACTATTGTGGAGGCTTCTATTCAGCTTCCTCCTTCCCTTTTCTCACCA
AAGCAAAAAAGAGAACTCAGACCAACTGATGACTCTCTTTACAAGCTTCAACTCATTGCA
TTCCGCAATGGAAAGCTTTTTCCAGCCACTGGAAATTCAACAAATTTGGCTGATGATGGA
AAACGACGTACTGTGGTTACCCCTGTGATTCTCACCAAAATAGATGGTGTGAATGTAGAT
ACCCACCACATCCCTGTTAATGTGACACTGCGTCGAATTGCACATGGAGCAGATGCTGTT
GCAGCCCGGTGGGATTTCGATTTGCTGAACGGACAAGGAGGCTGGAAGTCAGATGGGTGC
CATATACTCTATTCAGATGAAAATATCACTACGATTCAGTGCTACTCCCTTAGTAACTAT
GCAGTTTTAATGGATTTGACGGGATCTGAACTATACACCCAGGCGGCCAGCCTCCTGCAT
CCTGTGGTTTATACTACCGCTATCATTCTCCTCTTATGTCTCTTAGCCGTCATTGTCAGT
TACATATACCATCACAGTTTGATTAGAATCAGCCTCAAGAGCTGGCACATGCTTGTGAAC
TTGTGCTTTCATATTTTCCTAACCTGTGTGGTCTTTGTGGGAGGAATAACCCAGACTAGG
AATGCCAGCATCTGCCAAGCAGTTGGGATAATTCTTCACTATTCCACCCTTGCCACAGTA
CTATGGGTAGGAGTGACAGCTCGAAATATCTACAAACAAGTCACTAAAAAAGCTAAAAGA
TGCCAGGATCCTGATGAACCACCACCTCCACCAAGACCAATGCTCAGATTTTACCTGATT
GGTGGTGGTATCCCCATCATTGTTTGCGGCATAACTGCAGCAGCGAACATTAAGAATTAC
GGCAGTCGGCCAAACGCACCCTATTGCTGGATGGCATGGGAACCCTCCTTGGGAGCCTTC
TATGGGCCAGCCAGCTTCATCACTTTTGTAAACTGCATGTACTTTCTGAGCATATTTATT
CAGTTGAAAAGACACCCTGAGCGCAAATATGAGCTTAAGGAGCCCACGGAGGAGCAACAG
AGATTGGCAGCCAATGAAAATGGCGAAATAAATCATCAGGATTCAATGTCTTTGTCTCTG
ATTTCTACATCAGCCTTGGAAAATGAGCACACTTTTCATTCTCAGCTCTTGGGGGCCAGC
CTTACTTTGCTCTTATATGTTGCACTGTGGATGTTTGGGGCTTTGGCTGTTTCTTTGTAT
TACCCTTTGGACTTGGTTTTTAGCTTCGTTTTTGGAGCCACAAGTTTAAGCTTCAGTGCG
TTCTTCGTGGTCCACCATTGTGTTAATAGGGAGGATGTTAGACTTGCGTGGATCATGACT
TGCTGCCCAGGACGGAGCTCGTATTCAGTGCAAGTCAACGTCCAGCCCCCCAACTCTAAT
GGGACGAATGGAGAGGCACCCAAATGCCCCAATAGCAGTGCGGAGTCTTCATGCACAAAC
AAAAGTGCTTCAAGCTTCAAAAATTCCTCCCAGGGCTGCAAATTAACAAACTTGCAGGCG
GCTGCAGCTCAGTGCCATGCCAATTCTTTACCTTTGAACTCCACCCCTCAGCTTGATAAT
AGTCTGACAGAACATTCAATGGACAATGATATTAAAATGCACGTGGCGCCTTTAGAAGTT
CAGTTTCGAACAAATGTGCACTCAAGCCGCCACCATAAAAACAGAAGTAAAGGACACCGG
GCAAGCCGACTCACAGTCCTGAGAGAATATGCCTACGATGTCCCAACGAGCGTGGAAGGA
AGCGTGCAGAACGGCTTACCTAAAAGCCGGCTGGGCAATAACGAAGGACACTCGAGGAGC
CGAAGAGCTTATTTAGCCTACAGAGAGAGACAGTACAACCCACCCCAGCAAGACAGCAGC
GATGCTTGTAGCACACTTCCCAAAAGTAGCAGAAATTTTGAAAAGCCAGTTTCAACCACT
AGTAAAAAAGATGCGTTAAGGAAGCCAGCTGTGGTTGAACTTGAAAATCAGCAAAAATCT
TATGGCCTCAACTTGGCCATTCAGAATGGACCAATTAAAAGCAATGGGCAGGAGGGACCC
TTGCTCGGTACCGATAGCACTGGCAATGTTAGGACTGGATTATGGAAACACGAAACTACT
GTG
Protein sequence predicted based on nucleotide sequence
MEPPGRRRGRAQPPLLLPLSLLALLALLGGGGGGGAAALPAGCKHDGRPRGAGRAAGAAE GKVVCSSLELAQVLPPDTLPNRTVTLILSNNKISELKNGSFSGLSLLERLDLRNNLISSI DPGAFWGLSSLKRLDLTNNRIGCLNADIFRGLTNLVRLNLSGNLFSSLSQGTFDYLASLR SLEFQTEYLLCDCNILWMHRWVKEKNITVRDTRCVYPKSLQAQPVTGVKQELLTCDPPLE LPSFYMTPSHRQVVFEGDSLPFQCMASYIDQDMQVLWYQDGRIVETDESQGIFVEKNMIH NCSLIASALTISNIQAGSTGNWGCHVQTKRGNNTRTVDIVVLESSAQYCPPERVVNNKGD FRWPRTLAGITAYLQCTRNTHGSGIYPGNPQDERKAWRRCDRGGFWADDDYSRCQYANDV TRVLYMFNQMPLNLTNAVATARQLLAYTVEAANFSDKMDVIFVAEMIEKFGRFTKEEKSK ELGDVMVDIASNIMLADERVLWLAQREAKACSRIVQCLQRIATYRLAGGAHVYSTYSPNI ALEAYVIKSTGFTGMTCTVFQKVAASDRTGLSDYGRRDPEGNLDKQLSFKCNVSNTFSSL ALKNTIVEASIQLPPSLFSPKQKRELRPTDDSLYKLQLIAFRNGKLFPATGNSTNLADDG KRRTVVTPVILTKIDGVNVDTHHIPVNVTLRRIAHGADAVAARWDFDLLNGQGGWKSDGC HILYSDENITTIQCYSLSNYAVLMDLTGSELYTQAASLLHPVVYTTAIILLLCLLAVIVS YIYHHSLIRISLKSWHMLVNLCFHIFLTCVVFVGGITQTRNASICQAVGIILHYSTLATV LWVGVTARNIYKQVTKKAKRCQDPDEPPPPPRPMLRFYLIGGGIPIIVCGITAAANIKNY GSRPNAPYCWMAWEPSLGAFYGPASFITFVNCMYFLSIFIQLKRHPERKYELKEPTEEQQ RLAANENGEINHQDSMSLSLISTSALENEHTFHSQLLGASLTLLLYVALWMFGALAVSLY YPLDLVFSFVFGATSLSFSAFFVVHHCVNREDVRLAWIMTCCPGRSSYSVQVNVQPPNSN GTNGEAPKCPNSSAESSCTNKSASSFKNSSQGCKLTNLQAAAAQCHANSLPLNSTPQLDN SLTEHSMDNDIKMHVAPLEVQFRTNVHSSRHHKNRSKGHRASRLTVLREYAYDVPTSVEG SVQNGLPKSRLGNNEGHSRSRRAYLAYRERQYNPPQQDSSDACSTLPKSSRNFEKPVSTT SKKDALRKPAVVELENQQKSYGLNLAIQNGPIKSNGQEGPLLGTDSTGNVRTGLWKHETT V
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
266
End on reference sequence
4231
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
