Gene:
Gene Symbol:
ATP13A5
Gene Name:
ATPase type 13A5
Original Clone ID:
FLH269897.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297129
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAAGAGAACAGTAAGAAGGACCATCGGGCTTTGCTCAACCAGGGAGAGGAGGATGAA
CTGGAGGTGTTTGGTTACCGGGACCACAATGTACGGAAAGCCTTCTGCCTTGTCGCATCC
GTGCTGACCTGTGGGGGCCTTCTGCTGGTGTTCTACTGGAGACCCCAGTGGAGAGTGTGG
GCCAACTGCATCCCATGCCCCTTGCAAGAAGCAGACACTGTTTTGCTGAGGACAACAGAC
GAATTTCAAAGATATATGAGGAAGAAGGTATTCTGCCTCTACTTATCCACACTGAAGTTT
CCTGTAAGCAAGAAGTGGGAAGAATCCCTGGTGGCTGACCGCCACTCTGTCATAAACCAA
GCCTTAATAAAGCCAGAATTAAAACTGCGGTGCATGGAAGTGCAGAAAATCAGGTATGTT
TGGAACGACCTGGAGAAGCGGTTTCAGAAAGTTGGGTTGCTAGAAGACAGCAATTCCTGC
TCTGACATCCATCAGACATTTGGATTGGGTCTGACCAGTGAAGAGCAAGAGGTCAGAAGA
TTAGTGTGTGGGCCCAACGCCATTGAGGTTGAAATCCAACCCATATGGAAGCTGCTTGTT
AAACAGGTTTTAAATCCATTCTATGTGTTCCAAGCCTTCACCCTAACTTTGTGGCTGTCT
CAAGGTTACATAGAATACTCTGTGGCCATCATCATTTTGACTGTTATCTCCATTGTCTTA
AGTGTGTATGATTTGCGACAGCAATCAGTTAAGCTGCATAACCTCGTGGAGGACCACAAC
AAAGTCCAGGTTACAATCATTGTAAAAGACAAAGGTTTGGAGGAGCTGGAATCCCGTCTC
TTGGTTCCCGGAGACATTCTTATTCTTCCAGGAAAATTTTCATTGCCATGTGATGCTGTT
TTGATTGATGGAAGCTGCGTGGTGAATGAAGGCATGCTTACAGGAGAAAGTATACCTGTT
ACAAAGACACCATTGCCCCAGATGGAGAACACTATGCCTTGGAAATGTCACAGTTTGGAG
GATTATAGGAAACACGTCCTTTTCTGTGGAACAGAAGTTATCCAGGTCAAGCCCTCTGGG
CAGGGGCCTGTACGAGCAGTCGTTTTGCAAACAGGTTACAATACAGCCAAAGGGGACTTA
GTGAGATCCATCCTGTACCCCCGGCCTCTGAACTTCAAACTATACAGCGATGCCTTCAAG
TTCATCGTGTTCCTGGCCTGCCTTGGTGTCATGGGTTTTTTCTATGCCCTAGGGGTATAT
ATGTACCATGGAGTTCCTCCAAAAGATACTGTGACCATGGCCCTGATCCTCCTCACCGTG
ACTGTCCCTCCAGTGCTGCCAGCTGCCCTGACCATAGGCAACGTGTATGCTCAGAAGAGA
CTGAAGAAAAAGAAAATCTTCTGTATCTCCCCACAGAGAATCAACATGTGTGGGCAAATA
AACCTCGTGTGCTTTGACAAAACTGGCACTCTCACTGAAGATGGGCTGGACCTCTGGGGG
ACTGTCCCTACTGCTGACAACTGCTTCCAGGAAGCCCACAGCTTTGCCTCAGGCCAGGCT
GTGCCATGGAGCCCACTGTGTGCGGCCATGGCCAGCTGCCACTCTCTGATCCTTCTCAAT
GGGACCATCCAGGGAGACCCTCTGGACCTCAAAATGTTTGAGGGCACTGCCTGGAAAATG
GAAGATTGCATTGTAGACTCCTGCAAATTTGGGACGTCAGTTTCAAACATCATAAAACCA
GGACCAAAAGCCAGTAAGAGTCCAGTGGAAGCCATCATCACCTTGTGCCAGTTTCCATTT
TCCTCGAGCCTGCAGAGGATGTCCGTGATCGCTCAGCTAGCTGGGGAGAATCATTTCCAT
GTCTACATGAAAGGTGCCCCAGAAATGGTGGCCAGGTTCTGCAGATCTGAAACAGTGCCC
AAGAATTTCCCACAGGAACTGAGGAGTTACACGGTGCAAGGCTTCCGTGTCATTGCTCTT
GCCCACAAAACCTTAAAGATGGGGAATCTTTCAGAAGTCGAGCACTTAGCCAGAGAAAAA
GTGGAGTCAGAGTTAACATTTCTGGGACTTCTCATCATGGAGAATCGCTTGAAAAAAGAA
ACCAAACTGGTCTTGAAGGAACTGAGTGAGGCCCGTATCAGGACTGTGATGATTACAGGT
GATAACCTTCAAACGGCCATTACTGTTGCAAAGAATTCTGAAATGATCCCTCCAGGCAGC
CAAGTGATCATTGTTGAGGCCGATGAACCAGAAGAATTTGTTCCTGCCTCTGTGACCTGG
CAGCTGGTGGAGAACCAAGAGACTGGACCTGGGAAGAAAGAAATCTACATGCATACTGGA
AACAGTTCAACCCCTCGTGGGGAAGGAGGAAGCTGTTACCATTTTGCAATGAGTGGGAAA
TCATACCAAGTGATATTTCAGCATTTCAACAGCTTGCTTCCAAAAATTCTGGTGAATGGA
ACAGTTTTTGCAAGAATGTCTCCTGGGCAGAAATCAAGCCTTATTGAAGAATTTCAGAAA
TTAAATTATTATGTGGGCATGTGTGGAGATGGAGCTAACGACTGTGGGGCTTTGAAAGCG
GCTCATGCAGGCATTTCATTATCAGAGCAGGAAGCATCTGTGGCATCCCCTTTTACCTCT
AAAACAACCAACATCCAGTGTGTGCCTCATCTCATCAGAGAAGGCCGAGCTGCTCTGGTT
TCATCCTTTGGAGTATTTAAATACTTGACCATGTACGGCATAATCCAGTTTATCAGTGCA
TTACTGCTCTATTGGCAACTACAACTCTTTGGAAATTACCAGTATCTCATGCAAGATGTA
GCCATTACTTTGATGGTCTGTTTAACAATGAGTTCAACTCATGCCTACCCAAAGCTGGCT
CCATATAGACCAGCAGGACAGCTCCTTTCTCCCCCTTTACTGCTTTCAATATTTTTGAAT
TCCTGTTTCTCCTGCATTGTGCAGATCAGTGCATTTCTCTATGTGAAGCAGCAGCCTTGG
TATTGTGAGGTCTACCAATACAGTGAGTGTTTTCTGGCCAACCAAAGTAATTTTTCAACA
AATGTGAGTTTGGAAAGAAACTGGACTGGAAATGCAACTCTGATTCCTGGTTCAATTTTA
AGTTTTGAGACCACCACACTGTGGCCCATCACCACCATCAACTATATCACAGTAGCATTC
ATCTTTTCTAAGGGAAAGCCATTTCGAAAACCCATCTATACAAACTATATATTTTCATTT
CTGCTGCTAGCTGCCTTGGGCCTCACAATTTTCATTCTGTTTTCTGACTTTCAAGTTATA
TACCGTGGAATGGAGTTGATCCCAACCATAACATCGTGGAGGGTTTTAATTTTGGTGGTA
GCCCTCACCCAATTCTGTGTGGCTTTCTTTGTAGAGGATTCCATCCTTCAAAATCATGAA
CTCTGGCTGTTGATCAAAAGAGAATTTGGATTCTACTCTAAAAGTCAATATAGGACTTGG
CAAAAGAAGCTAGCAGAAGACTCAACCTGGCCTCCCATAAACAGGACAGATTATTCAGGT
GATGGCAAAAATGGATTCTACATCAACGGAGGCTATGAAAGCCATGAACAGATTCCAAAA
AGAAAACTCAAATTGGGAGGCCAACCCACAGAACAGCATTTCTGGGCCAGGCTG
Protein sequence predicted based on nucleotide sequence
MEENSKKDHRALLNQGEEDELEVFGYRDHNVRKAFCLVASVLTCGGLLLVFYWRPQWRVW ANCIPCPLQEADTVLLRTTDEFQRYMRKKVFCLYLSTLKFPVSKKWEESLVADRHSVINQ ALIKPELKLRCMEVQKIRYVWNDLEKRFQKVGLLEDSNSCSDIHQTFGLGLTSEEQEVRR LVCGPNAIEVEIQPIWKLLVKQVLNPFYVFQAFTLTLWLSQGYIEYSVAIIILTVISIVL SVYDLRQQSVKLHNLVEDHNKVQVTIIVKDKGLEELESRLLVPGDILILPGKFSLPCDAV LIDGSCVVNEGMLTGESIPVTKTPLPQMENTMPWKCHSLEDYRKHVLFCGTEVIQVKPSG QGPVRAVVLQTGYNTAKGDLVRSILYPRPLNFKLYSDAFKFIVFLACLGVMGFFYALGVY MYHGVPPKDTVTMALILLTVTVPPVLPAALTIGNVYAQKRLKKKKIFCISPQRINMCGQI NLVCFDKTGTLTEDGLDLWGTVPTADNCFQEAHSFASGQAVPWSPLCAAMASCHSLILLN GTIQGDPLDLKMFEGTAWKMEDCIVDSCKFGTSVSNIIKPGPKASKSPVEAIITLCQFPF SSSLQRMSVIAQLAGENHFHVYMKGAPEMVARFCRSETVPKNFPQELRSYTVQGFRVIAL AHKTLKMGNLSEVEHLAREKVESELTFLGLLIMENRLKKETKLVLKELSEARIRTVMITG DNLQTAITVAKNSEMIPPGSQVIIVEADEPEEFVPASVTWQLVENQETGPGKKEIYMHTG NSSTPRGEGGSCYHFAMSGKSYQVIFQHFNSLLPKILVNGTVFARMSPGQKSSLIEEFQK LNYYVGMCGDGANDCGALKAAHAGISLSEQEASVASPFTSKTTNIQCVPHLIREGRAALV SSFGVFKYLTMYGIIQFISALLLYWQLQLFGNYQYLMQDVAITLMVCLTMSSTHAYPKLA PYRPAGQLLSPPLLLSIFLNSCFSCIVQISAFLYVKQQPWYCEVYQYSECFLANQSNFST NVSLERNWTGNATLIPGSILSFETTTLWPITTINYITVAFIFSKGKPFRKPIYTNYIFSF LLLAALGLTIFILFSDFQVIYRGMELIPTITSWRVLILVVALTQFCVAFFVEDSILQNHE LWLLIKREFGFYSKSQYRTWQKKLAEDSTWPPINRTDYSGDGKNGFYINGGYESHEQIPK RKLKLGGQPTEQHFWARL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3657
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
