Gene:
Gene Symbol:
IQGAP2
Gene Name:
IQ motif containing GTPase activating protein 2
Original Clone ID:
FLH269921.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297153
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGAT
GAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATAT
CTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCA
CCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAG
TTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTAT
AAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATG
GAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAA
AACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGA
ATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGT
AATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGT
GGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCC
ATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCA
AATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGG
GATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAA
GAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAA
GTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAG
AATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCT
GCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTG
GCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAG
GCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGC
TTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAA
CTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATT
GATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATC
AATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACT
GCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCAT
GCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAG
ATACAGCAAGCCGTCGATGATGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACT
CTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTA
TTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTAT
GATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGG
CTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGT
TCCTGGGTCACACCTGAATCATGCTTGTATAAAGAATCATGGCTCACAGGAAAAGAAATC
GAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCA
GAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTT
GAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTT
TGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAAT
ACTGATTCTATTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTAT
CTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTG
TTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCA
TTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAG
GAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAG
CTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATC
ACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATG
GAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAA
ACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCT
AAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTC
ACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAA
ACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGT
AACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACC
CTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATC
AACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGA
GAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTG
AAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAAT
TCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTG
AAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTT
GGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGAT
ATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCA
GTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCAT
CTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAA
GAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTG
GTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCA
CTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTG
CTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGAC
CCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTT
GTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATG
ATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACA
GAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTG
AGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATT
GAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACG
TTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATT
GCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAA
CTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAAT
TATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGA
TCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAG
TACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACA
AACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGAT
GTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTA
CTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAAT
GTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAG
Protein sequence predicted based on nucleotide sequence
MPHEELPSLQRPRYGSIVDDERLSAEEMDERRRQNIAYEYLCHLEEAKRWMEVCLVEELP PTTELEEGLRNGVYLAKLAKFFAPKMVSEKKIYDVEQTRYKKSGLHFRHTDNTVQWLRAM ESIGLPKIFYPETTDVYDRKNIPRMIYCIHALSLYLFKLGIAPQIQDLLGKVDFTEEEIS NMRKELEKYGIQMPSFSKIGGILANELSVDEAALHAAVIAINEAVEKGIAEQTVVTLRNP NAVLTLVDDNLAPEYQKELWDAKKKKEENARLKNSCISEEERDAYEELLTQAEIQGNINK VNRQAAVDHINAVIPEGDPENTLLALKKPEAQLPAVYPFAAAMYQNELFNLQKQNTMNYL AHEELLIAVEMLSAVALLNQALESNDLVSVQNQLRSPAIGLNNLDKAYVERYANTLLSVK LEVLSQGQDNLSWNEIQNCIDMVNAQIQEENDRVVAVGYINEAIDEGNPLRTLETLLLPT ANISDVDPAHAQHYQDVLYHAKSQKLGDSESVSKVLWLDEIQQAVDDANVDEDRAKQWVT LVVDVNQCLEGKKSSDILSVLKSSTSNANDIIPECADKYYDALVKAKELKSERVSSDGSW LKLNLHKKYDYYYNTDSKESSWVTPESCLYKESWLTGKEIEDIIEEVTVGYIRENIWSAS EELLLRFQATSSGPILREEFEARKSFLHEQEENVVKIQAFWKGYKQRKEYMHRRQTFIDN TDSIVKIQSWFRMATARKSYLSRLQYFRDHNNEIVKIQSLLRANKARDDYKTLVGSENPP LTVIRKFVYLLDQSDLDFQEELEVARLREEVVTKIRANQQLEKDLNLMDIKIGLLVKNRI TLEDVISHSKKLNKKKGGEMEILNNTDNQGIKSLSKERRKTLETYQQLFYLLQTNPLYLA KLIFQMPQNKSTKFMDTVIFTLYNYASNQREEYLLLKLFKTALEEEIKSKVDQVQDIVTG NPTVIKMVVSFNRGARGQNTLRQLLAPVVKEIIDDKSLIINTNPVEVYKAWVNQLETQTG EASKLPYDVTTEQALTYPEVKNKLEASIENLRRVTDKVLNSIISSLDLLPYGLRYIAKVL KNSIHEKFPDATEDELLKIVGNLLYYRYMNPAIVAPDGFDIIDMTAGGQINSDQRRNLGS VAKVLQHAASNKLFEGENEHLSSMNNYLSETYQEFRKYFKEACNVPEPEEKFNMDKYTDL VTVSKPVIYISIEEIISTHSLLLEHQDAIAPEKNDLLSELLGSLGEVPTVESFLGEGAVD PNDPNKANTLSQLSKTEISLVLTSKYDIEDGEAIDSRSLMIKTKKLIIDVIRNQPGNTLT EILETPATAQQEVDHATDMVSRAMIDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRT LEQTGHVSSENKYQDILNEIAKDIRNQRIYRKLRKAELAKLQQTLNALNKKAAFYEEQIN YYDTYIKTCLDNLKRKNTRRSIKLDGKGEPKGAKRAKPVKYTAAKLHEKGVLLDIDDLQT NQFKNVTFDIIATEDVGIFDVRSKFLGVEMEKVQLNIQDLLQMQYEGVAVMKMFDKVKVN VNLLIYLLNKKFYGK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
223
End on reference sequence
4950
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
