Gene:
Gene Symbol:
BCORL1
Gene Name:
BCL6 corepressor-like 1
Original Clone ID:
FLH269926.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297158
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATCTCTACAGCACCGCTCTACAGCGGCGTGCACAACTGGACCAGTTCTGACCGGATT
CGCATGTGTGGCATCAACGAGGAGAGAAGAGCACCTCTTTCTGATGAGGAGTCAACGACA
GGCGACTGCCAGCACTTTGGATCTCAGGAGTTTTGTGTCAGCAGCAGTTTTTCCAAGGTG
GAGCTCACGGCAGTTGGAAGTGGCAGCAATGCCCGGGGGGCAGACCCAGATGGCAGTGCT
ACAGAAAAACTTGGGCACAAGTCAGAAGACAAGCCTGACGATCCCCAGCCAAAAATGGAC
TACGCTGGGAACGTGGCAGAGGCTGAGGGCTTCTTGGTGCCCCTGAGCAGCCCAGGAGAC
GGGCTCAAGCTTCCCGCATCTGACAGCGCCGAGGCCAGCAACAGCAGGGCCGACTGCTCC
TGGACTCCACTCAACACCCAAATGAGCAAACAGGTTGACTGCTCACCCGCCGGAGTAAAG
GCTTTGGACTCTCGGCAAGGTGTTGGAGAGAAGAATACTTTCATTTTGGCAACTCTGGGA
ACTGGAGTCCCTGTGGAGGGGACCCTGCCCCTGGTTACCACTAACTTCAGTCCTCTGCCA
GCCCCTATCTGTCCCCCTGCTCCCGGTTCGGCCTCTGTGCCCCACTCTGTTCCAGATGCA
TTCCAGGTTCCCCTCTCCGTCCCTGCCCCAGTCCCCCATTCAGGGCTTGTTCCAGTCCAA
GTTGCCACTTCGGTTCCAGCTCCTTCCCCTCCCTTAGCACCTGTCCCGGCTCTGGCTCCA
GCGCCACCGTCAGTGCCCACGCTCATCTCTGACTCGAACCCCCTTTCTGTTTCGGCCTCA
GTCTTGGTGCCTGTGCCAGCTTCTGCTCCCCCTTCAGGCCCGGTTCCCTTGTCGGCTCCA
GCTCCTGCCCCGCTTTCAGTCCCAGTTTCAGCTCCTCCCTTGGCTCTCATCCAGGCTCCT
GTGCCCCCTTCAGCTCCGACCTTGGTTCTCGCTCCCGTCCCCACTCCGGTTCTGGCTCCC
ATGCCAGCATCCACGCCTCCAGCGGCCCCTGCCCCTCCGTCTGTGCCCATGCCCACTCCA
ACCCCATCTTCCGGCCCACCTTCTACCCCCACCCTCATCCCCGCCTTTGCTCCTACACCG
GTGCCTGCACCCACCCCAGCCCCCATCTTTACTCCAGCCCCTACACCCATGCCTGCTGCC
ACGCCAGCTGCCATTCCCACCTCTGCACCCATCCCGGCCTCCTTCAGTTTGAGTAGAGTG
TGCTTTCCTGCAGCTCAGGCACCAGCTATGCAAAAAGTCCCCCTGTCCTTTCAGCCAGGG
ACAGTGCTGACCCCGAGCCAGCCGCTGGTATATATCCCGCCTCCAAGCTGTGGGCAGCCA
CTCAGTGTGGCCACACTGCCAACCACTCTAGGGGTTTCCTCCACTCTTACGCTCCCTGTC
CTGCCGTCCTACCTGCAGGACAGGTGTCTCCCAGGCGTGCTAGCCTCCCCCGAGCTCCGT
TCTTACCCGTATGCATTTTCTGTGGCCCGGCCTCTGACTTCGGATTCCAAGCTGGTATCT
CTGGAGGTGAACAGGCTCCCCTGCACTTCCCCATCCGGTAGCACCACCACCCAGCCTGCA
CCCGATGGGGTCCCTGGGCCTTTGGCAGATACCTCCCTTGTTACTGCTTCTGCCAAGGTG
CTTCCAACTCCACAGCCTCTGCTGCCAGCCCCCAGTGGGAGCTCAGCCCCACCGCACCCC
GCCAAGATGCCCAGTGGCACCGAGCAGCAAACAGAAGGGACTTCCGTTACCTTCTCTCCT
CTTAAGTCACCGCCACAGCTGGAACGAGAGATGGCCTCTCCACCTGAGTGCAGCGAGATG
CCCCTTGATCTGTCCTCCAAGTCCAACCGCCAGAAGCTTCCATTGCCGAACCAGCGCAAG
ACACCCCCCATGCCTGTGTTGACCCCCGTGCACACCAGCAGCAAGGCCCTCCTCTCCACA
GTCCTGTCTAGGTCTCAGCGCACAACCCAGGCTGCCGGTGGCAATGTCACCTCCTGCCTG
GGCTCCACTTCCTCGCCCTTTGTCATCTTTCCCGAGATCGTGAGGAATGGGGACCCGAGC
ACCTGGGTGAAGAACTCAACTGCACTGATCAGCACCATTCCTGGCACCTACGTGGGAGTG
GCCAACCCAGTGCCTGCATCCCTGCTGCTGAACAAAGACCCCAACCTGGGCCTCAACCGT
GACCCCCGCCATCTCCCCAAGCAGGAGCCCATCTCCATCATTGATCAAGGAGAGCCTAAG
GGCACTGGTGCCACGTGTGGCAAAAAGGGCAGCCAGGCTGGTGCTGAGGGACAGCCAAGC
ACAGTGAAACGATATACTCCAGCCCGCATTGCCCCTGGGCTGCCAGGGTGCCAAACCAAG
GAACTCTCTTTGTGGAAACCCACGGGGCCGGCAAATATTTATCCCCGGTGTTCAGTCAAT
GGGAAACCTACCAGCACCCAGGTCCTGCCTGTTGGCTGGTCCCCGTACCACCAGGCGTCT
CTGCTTTCCATTGGCATTTCCAGTGCCGGGCAGCTGACCCCCAGTCAGGGGGCGCCCATC
AGGCCCACCAGCGTTGTTTCGGAGTTTTCTGGTGTGCCATCTCTCAGCTCCAGCGAAGCC
GTGCACGGACTTCCTGAGGGGCAACCACGGCCTGGGGGCTCCTTCGTTCCAGAGCAGGAC
CCTGTTACAAAGAACAAAACTTGCCGGATTGCTGCCAAGCCTTATGAAGAACAAGTCAAT
CCTGTCCTCTTGACCCTCAGCCCTCAGACTGGGACCCTGGCACTGTCTGTTCAGCCTAGC
GGTGGGGACATTCGAATGAATCAGGGGCCTGAGGAATCAGAGAGCCACCTCTGCTCTGAC
AGCACTCCTAAGATGGAAGGCCCCCAGGGGGCTTGTGGCCTGAAGCTGGCAGGAGACACG
AAGCCTAAGAACCAAGTGCTGGCCACCTACATGTCCCATGAGCTGGTCCTGGCCACCCCC
CAGAACCTGCCTAAGATGCCTGAGCTGCCTTTGCTACCTCACGACAGCCACCCCAAGGAA
CTTATATTGGACGTGGTTCCGAGCAGCAGGAGGGGCTCCAGCACAGAGCGCCCACAGCTT
GGAAGCCAGGTGGATCTGGGGCGAGTGAAAATGGAGAAGGTGGATGGTGATGTGGTCTTC
AATTTAGCCACCTGCTTCCGGGCTGATGGCCTCCCAGTGGCTCCCCAGAGGGGCCAAGCT
GAAGTTCGGGCTAAGGCCGGGCAGGCTCGAGTGAAACAGGAAAGCGTAGGGGTCTTTGCT
TGCAAGAACAAGTGGCAGCCAGATGATGTGACGGAATCTCTGCCGCCCAAGAAGATGAAG
TGCGGCAAAGAGAAGGACAGTGAAGAGCAGCAGCTCCAGCCACAAGCCAAGGCCGTGGTC
CGGAGTTCCCACAGACCCAAGTGCCGGAAGCTGCCCAGTGACCCCCAGGAATCCACCAAG
AAAAGCCCCAGGGGGGCTTCAGATTCAGGAAAAGAGCACAATGGAGTCAGGGGAAAGCAC
AAGCACCGGAAGCCGACAAAGCCGGAGTCCCAGTCTCCAGGAAAACGAGCCGACAGCCAC
GAGGAAGGTTCCTTGGAAAAGAAAGCAAAGAGCAGTTTCCGTGACTTTATTCCTGTGGTT
CTGAGCACCCGCACGCGCAGTCAGTCTGGAAGCATCTGTAGCTCCTTTGCTGGCATGGCA
GACAGTGACATGGGAAGCCAGGAAGTCTTCCCCACAGAAGAAGAAGAGGAGGTAACCCCC
ACCCCAGCTAAGCGTCGAAAGGTGAGAAAGACCCAACGGGACACCCAGTATCGCAGCCAC
CATGCCCAGGACAAGTCTCTGCTGAGCCAGGGCCGAAGGCACCTGTGGCGAGCCCGAGAA
ATGCCCTGGAGGACAGAGGCTGCCCGGCAAATGTGGGACACCAATGAGGAGGAGGAGGAA
GAAGAGGAGGAGGGCCTGCTGAAGAGGAAGAAACGAAGACGGCAGAAGAGCCGAAAATAT
CAGACTGGGGAGTACCTGACAGAGCAAGAAGACGAGCAGCGGCGGAAAGGGAGAGCAGAT
TTAAAGGCCCGTAAGCAGAAGACTTCCTCCTCCCAAAGTTTGGAGCACCGCCTCAGGAAC
AGGAACCTTCTCTTGCCCAACAAAGTCCAGGGGATCTCGGATTCACCAAACGGTTTCCTC
CCAAATAACCTGGAAGAGCCAGCCTGCCTTGAAAATTCAGAAAAGCCATCAGGAAAACGA
AAGTGCAAGACCAAGCACATGGCAACCGTCTCAGAAGAGGCAAAGGATGTTGTTCTCTAC
TGCCTCCAGAAAGACAGTGAAGATGTGAATCACCGTGACAATGCTGGCTACACAGCCCTG
CATGAGGCTTGTTCCCGGGGCTGGACCGACATCCTGAACATCCTGCTGGAGCACGGGGCC
AACGTGAACTGCAGTGCGCAGGACGGCACGAGGCCAGTTCATGATGCGGTGGTCAATGAC
AACCTGGAGACCATCTGGCTCCTGCTGTCCTATGGGGCCGATCCCACACTGGCTACCTAC
TCGGGTCAGACAGCCATGAAGCTGGCCAGCAGCGACACCATGAAGCGCTTTCTCAGTGAT
CACCTCTCGGATCTTCAGGGCCGGGCAGAGGGTGATCCCGGTGTATCCTGGGATTTTTAC
AGCAGTTCTGTGTTGGAGGAAAAAGACGGGTTTGCCTGTGACCTCCTACATAATCCTCCT
GGGAGCTCAGATCAAGAAGGAGACGATCCGATGGAGGAGGATGATTTCATGTTTGAACTC
TCAGACAAGCCTCTTCTCCCTTGCTACAACCTCCAAGTGTCAGTGTCCCGCGGGCCCTGC
AACTGGTTCCTCTTTTCCGATGTCTTGAAGAGGCTGAAGCTTTCCTCGAGGATCTTTCAG
GCCCGGTTCCCGCACTTTGAAATCACCACCATGCCCAAGGCCGAGTTCTACAGGCAGGTG
GCCTCCAGTCAGCTGCTGACCCCTGCCGAGAGGCCTGGAGGCTTGGACGACAGATCCCCC
CCAGGCTCCTCTGAGACTGTGGAGCTGGTGCGGTACGAGCCAGACCTACTTCGGCTCCTA
GGGTCCGAGGTGGAATTCCAGTCTTGCAACAGT
Protein sequence predicted based on nucleotide sequence
MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKV ELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGD GLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLG TGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQ VATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAP APAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAAPAPPSVPMPTP TPSSGPPSTPTLIPAFAPTPVPAPTPAPIFTPAPTPMPAATPAAIPTSAPIPASFSLSRV CFPAAQAPAMQKVPLSFQPGTVLTPSQPLVYIPPPSCGQPLSVATLPTTLGVSSTLTLPV LPSYLQDRCLPGVLASPELRSYPYAFSVARPLTSDSKLVSLEVNRLPCTSPSGSTTTQPA PDGVPGPLADTSLVTASAKVLPTPQPLLPAPSGSSAPPHPAKMPSGTEQQTEGTSVTFSP LKSPPQLEREMASPPECSEMPLDLSSKSNRQKLPLPNQRKTPPMPVLTPVHTSSKALLST VLSRSQRTTQAAGGNVTSCLGSTSSPFVIFPEIVRNGDPSTWVKNSTALISTIPGTYVGV ANPVPASLLLNKDPNLGLNRDPRHLPKQEPISIIDQGEPKGTGATCGKKGSQAGAEGQPS TVKRYTPARIAPGLPGCQTKELSLWKPTGPANIYPRCSVNGKPTSTQVLPVGWSPYHQAS LLSIGISSAGQLTPSQGAPIRPTSVVSEFSGVPSLSSSEAVHGLPEGQPRPGGSFVPEQD PVTKNKTCRIAAKPYEEQVNPVLLTLSPQTGTLALSVQPSGGDIRMNQGPEESESHLCSD STPKMEGPQGACGLKLAGDTKPKNQVLATYMSHELVLATPQNLPKMPELPLLPHDSHPKE LILDVVPSSRRGSSTERPQLGSQVDLGRVKMEKVDGDVVFNLATCFRADGLPVAPQRGQA EVRAKAGQARVKQESVGVFACKNKWQPDDVTESLPPKKMKCGKEKDSEEQQLQPQAKAVV RSSHRPKCRKLPSDPQESTKKSPRGASDSGKEHNGVRGKHKHRKPTKPESQSPGKRADSH EEGSLEKKAKSSFRDFIPVVLSTRTRSQSGSICSSFAGMADSDMGSQEVFPTEEEEEVTP TPAKRRKVRKTQRDTQYRSHHAQDKSLLSQGRRHLWRAREMPWRTEAARQMWDTNEEEEE EEEEGLLKRKKRRRQKSRKYQTGEYLTEQEDEQRRKGRADLKARKQKTSSSQSLEHRLRN RNLLLPNKVQGISDSPNGFLPNNLEEPACLENSEKPSGKRKCKTKHMATVSEEAKDVVLY CLQKDSEDVNHRDNAGYTALHEACSRGWTDILNILLEHGANVNCSAQDGTRPVHDAVVND NLETIWLLLSYGADPTLATYSGQTAMKLASSDTMKRFLSDHLSDLQGRAEGDPGVSWDFY SSSVLEEKDGFACDLLHNPPGSSDQEGDDPMEEDDFMFELSDKPLLPCYNLQVSVSRGPC NWFLFSDVLKRLKLSSRIFQARFPHFEITTMPKAEFYRQVASSQLLTPAERPGGLDDRSP PGSSETVELVRYEPDLLRLLGSEVEFQSCNS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
115
End on reference sequence
5250
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
