Gene:
Gene Symbol:
FBXO43
Gene Name:
F-box protein 43
Original Clone ID:
FLH269932.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297164
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGTTTTAAAGACAAAGATGAGAGAATTTCTTGTTTGGAAGCCTACGTAACTTTGACA
TCTAAGAGCTCAAGATTTACTGATGAAACAGAGATTTTGAAGATGTCGCAAAGGCACTCA
GGTCAAGCTGGCACTGAAGCAGGAAATGGGGCGGACTCTCCTCCAATTGTCAACTCCAAG
TACTCCACCTTCAGAGATTTTTGTTCCACATCTTCATTTCAAGATAGTGGCTACAATGAG
TTAAAATCTTGTAGCTTTGATAATATAGATAAAGAATATCTTGGAAAGAAAGAAAAAGGC
CCAACATTACTCTATGAGCACCCTGAAACTTCAGGCCTGGGCTTAACACATCCTTTAGAA
TCTCCCACTCAAAAAAAGAAATGTATCTTGCCTAGAAAGGAAAAGGATAAAACCCCAGAA
CTTTGTGAAACACCTAAAATCAGTGGGAAAAAATGTTTACCTCGCAGAAGGTTGAATGTA
TCTTTCGCTCTTCTAAAAGGGGACTTTGAATCACAAAATAGTTCTTTAGAAAGTAGTATA
AGCCAAGTTATCAACTTAGAAAAAAATATTCCAAGCAGTGCTTCAGGTTTTTCCAGGGCA
AATAATTTTAGCCCTTTAGTTACTAGCACTTTAAAAACAGAAGAAGTGACTTCATGCAGT
CAAAAATTGAGGCTTAATTTTTCTCAGCAAAAGACTTCCACAATTGATGATTCCAAAGAT
GATTGTAGCCTATTTGAAGTTGAATGTATATCTCCAATTCAGGGCAATAATTTTAAAGAC
TCTATCACACATGACTTTAGTGATAGCAGTTTATGCATTAATGATGAGAATGCATGTCCA
GAGCTCCTGGGCTCCTCTGTTAGTGGAACAACTTGTGGAACAGATGAGGACATATTTGTG
ACTCCGATAAGTAATCTTGTGGCAAACATTAGATTTAACGCAAGTCAAATACTTTCTCCT
TCACCTGAAGTGAGAGGCAGTATTTCAACGCCTGAAGACAGTGGTTTTAACTCACTTAGC
TTGGAGAAATCAGAAGATTCCCTGTCTGACCAGGAGGGTTCTTTTCAAGAACTACTGCAG
AAACATAAGGGGACTCCCAAAGTTGGGGACACCATAAGAAAGACAAGACATCTTGGAAGG
TCGAGAAGACTGTCCACCCTTCGGGAACAAAGCTCGCAGTCAGAGACAGAAGAGGAAAAG
CAGATTGTCCACCCTGACTCTGAAAAAAGAGCAGCAGCTGCTTCTGCCATCTCAGAGGGT
CAGCTGAGTAGTGATGAGAGTGGGGATTTGACCTTTAGCTTAAAGAATTTATCAAAGACC
CCAGCCTTGCAATTGGTACATGAGCTGTTCATGAAAAGCAAGAGGAAAAGATTACAGGAA
AATAGTGGACATGAATTCTTAGAGCAAGGGGATGGGGAGAAAATAGCTGTACTGCAGTGT
ATACTTGCAGGACTGATCGGCAAGAAAATGGGTATAGAAAAACTGGACATCTTAACAGAA
TTAAAATATAGAAATTTAAAGCATATTCTTGCTATGGTTTTAGAGTCCTTGACCGCAGAG
AGCCTATGCAGTGTTTGGAAAGTAAGCAGAAATTGGCGTGAAATTGTTGTTCAAGATAAA
AATGCAAATCGGAGGAGGAAATTTTATATCACACAACTGAAAACAGATTCTGAGGGGGCT
GTATTAAATGTCGAGGATGCTGCCACTCGGCTCCAGCTTTTAAATCGCTCAGCTTTAAGA
TCTGTGCAGGCACAGGCTAGGATACCTGGTTCTCAGAGAGAGCAAGGGTCAACATTATCT
CCCTGGGGAGAAGTTTTGACACCTCTAGCAAGCTCTTCTGTTACTCACTTAAGTAGTAAA
CAGGAAGAATATGTTAAGGTTGCCAAAACACTTTTTACTGATGAAGCATTAAAACCTTGC
CCAAGGTGCCAGTCCCCTGCTAAGTACCAGCCATATAAGAAAAGGGGACTGTGTAGCCGA
ACAGCCTGTGGTTTTGACTTTTGTGTGTTATGTCTGTGTGCTTATCATGGGTCTGAAGAA
TGTAGTAGAGGAGCAGCAAAGCCAAGAAATAGAAAAGATGCTCTCCCAGGAAGTGCCCAG
AGTAAGCGGAATTTAAAACGCCTC
Protein sequence predicted based on nucleotide sequence
MSFKDKDERISCLEAYVTLTSKSSRFTDETEILKMSQRHSGQAGTEAGNGADSPPIVNSK YSTFRDFCSTSSFQDSGYNELKSCSFDNIDKEYLGKKEKGPTLLYEHPETSGLGLTHPLE SPTQKKKCILPRKEKDKTPELCETPKISGKKCLPRRRLNVSFALLKGDFESQNSSLESSI SQVINLEKNIPSSASGFSRANNFSPLVTSTLKTEEVTSCSQKLRLNFSQQKTSTIDDSKD DCSLFEVECISPIQGNNFKDSITHDFSDSSLCINDENACPELLGSSVSGTTCGTDEDIFV TPISNLVANIRFNASQILSPSPEVRGSISTPEDSGFNSLSLEKSEDSLSDQEGSFQELLQ KHKGTPKVGDTIRKTRHLGRSRRLSTLREQSSQSETEEEKQIVHPDSEKRAAAASAISEG QLSSDESGDLTFSLKNLSKTPALQLVHELFMKSKRKRLQENSGHEFLEQGDGEKIAVLQC ILAGLIGKKMGIEKLDILTELKYRNLKHILAMVLESLTAESLCSVWKVSRNWREIVVQDK NANRRRKFYITQLKTDSEGAVLNVEDAATRLQLLNRSALRSVQAQARIPGSQREQGSTLS PWGEVLTPLASSSVTHLSSKQEEYVKVAKTLFTDEALKPCPRCQSPAKYQPYKKRGLCSR TACGFDFCVLCLCAYHGSEECSRGAAKPRNRKDALPGSAQSKRNLKRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2840
Start on reference sequence
714
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
