SCUBE1 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  SCUBE1
Gene Name:  signal peptide, CUB domain, EGF-like 1
Original Clone ID:
FLH269953.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 27620967
Title: A Pdx-1-regulated soluble factor activates rat and human islet cell proliferation
Authors:
The ORFeome Collaboration
HsCD00297185
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2964nts
Open reading frame: 1 to 2964

ATGGGCGCGGCGGCCGTGCGCTGGCACTTGTGCGTGCTGCTGGCCCTGGGCACACGCGGG
CGGCTGGCCGGGGGCAGCGGGCTCCCAGGGTCAGTCGACGTGGATGAGTGCTCAGAGGGC
ACAGATGACTGCCACATCGATGCCATCTGTCAGAACACGCCCAAGTCCTACAAATGCCTC
TGCAAGCCAGGCTACAAGGGGGAAGGCAAGCAGTGTGAAGACATTGACGAGTGTGAGAAT
GACTACTACAATGGGGGCTGTGTCCACGAGTGCATCAACATCCCGGGGAACTACAGGTGT
ACCTGCTTTGATGGCTTCATGCTGGCACACGATGGACACAACTGCCTGGATGTGGACGAG
TGTCAGGACAATAATGGTGGCTGCCAGCAGATCTGCGTCAATGCCATGGGCAGCTACGAG
TGTCAGTGCCACAGTGGCTTCTTCCTTAGTGACAACCAGCATACCTGCATCCACCGCTCC
AATGAGGGTATGAACTGCATGAACAAAGACCATGGCTGTGCCCACATCTGCCGGGAGACG
CCCAAAGGTGGGGTGGCCTGCGACTGCAGGCCCGGCTTTGACCTTGCCCAAAACCAGAAG
GACTGCACACTAACCTGTAATTATGGAAACGGAGGCTGCCAGCACAGCTGTGAGGACACA
GACACAGGCCCCACGTGTGGTTGCCACCAGAAGTACGCCCTCCACTCAGACGGTCGCACG
TGCATCGAGACGTGCGCAGTCAATAACGGAGGCTGCGACCGGACATGCAAGGACACAGCC
ACTGGCGTGCGATGCAGCTGCCCCGTTGGATTCACACTGCAGCCGGACGGGAAGACATGC
AAAGACATCAACGAGTGCCTGGTCAACAACGGAGGCTGCGACCACTTCTGCCGCAACACC
GTGGGCAGCTTCGAGTGCGGCTGCCGGAAGGGCTACAAGCTGCTCACCGACGAGCGCACC
TGCCAGGACATCGACGAGTGCTCCTTCGAGCGGACCTGTGACCACATCTGCATCAACTCC
CCGGGCAGCTTCCAGTGCCTGTGTCACCGCGGCTACATCCTCTACGGGACAACCCACTGC
GGAGATGTGGACGAGTGCAGCATGAGCAACGGGAGCTGTGACCAGGGCTGCGTCAACACC
AAGGGCAGCTACGAGTGCGTCTGTCCCCCGGGGAGGCGGCTCCACTGGAACGGGAAGGAT
TGCGTGGAGACAGGCAAGTGTCTTTCTCGCGCCAAGACCTCCCCCCGGGCCCAGCTGTCC
TGCAGCAAGGCAGGCGGTGTGGAGAGCTGCTTCCTTTCCTGCCCGGCTCACACACTCTTC
GTGCCAGACTCGGAAAATAGCTACGTCCTGAGCTGCGGAGTTCCAGGGCCGCAGGGCAAG
GCGCTGCAGAAACGCAACGGCACCAGCTCTGGCCTCGGGCCCAGCTGCTCAGATGCCCCC
ACCACCCCCATCAAACAGAAGGCCCGCTTCAAGATCCGAGATGCCAAGTGCCACCTCCGG
CCCCACAGCCAGGCACGAGCAAAGGAGACCGCCAGGCAGCCGCTGCTGGACCACTGCCAT
GTGACTTTCGTGACCCTCAAGTGTGACTCCTCCAAGAAGAGGCGCCGTGGCCGCAAGTCC
CCATCCAAGGAGGTGTCCCACATCACAGCAGAGTTTGAGATCGAGACAAAGATGGAAGAG
GCCTCAGACACATGCGAAGCGGACTGCTTGCGGAAGCGAGCAGAACAGAGCCTGCAGGCC
GCCATCAAGACCCTGCGCAAGTCCATCGGCCGGCAGCAGTTCTATGTCCAGGTCTCAGGC
ACTGAGTACGAGGTAGCCCAGAGGCCAGCCAAGGCGCTGGAGGGGCAGGGGGCATGTGGC
GCAGGCCAGGTGCTACAGGACAGCAAATGCGTTGCCTGTGGGCCTGGCACCCACTTCGGT
GGTGAGCTCGGCCAGTGTGTGTCATGTATGCCAGGAACATACCAGGACATGGAAGGCCAG
CTCAGTTGCACACCGTGCCCCAGCAGCGACGGGCTTGGTCTGCCTGGTGCCCGCAACGTG
TCGGAATGTGGAGGCCAGTGTTCTCCAGGCTTCTTCTCGGCCGATGGCTTCAAGCCCTGC
CAGGCCTGCCCCGTGGGCACGTACCAGCCTGAGCCCGGGCGCACCGGCTGCTTCCCCTGT
GGAGGGGGTTTGCTCACCAAACACGAAGGCACCACCTCCTTCCAGGACTGCGAGGCTAAA
GTGCACTGCTCCCCCGGCCACCACTACAACACCACCACCCACCGCTGCATCCGCTGCCCC
GTCGGCACCTACCAGCCCGAGTTTGGCCAGAACCACTGCATCACCTGTCCGGGCAACACC
AGCACAGACTTCGATGGCTCCACCAACGTCACACACTGCAAAAACCAGCACTGCGGCGGC
GAGCTTGGTGACTACACCGGCTACATCGAGTCCCCCAACTACCCTGGCGACTACCCAGCC
AACGCTGAATGCGTCTGGCACATCGCGCCTCCCCCAAAGCGCAGGATCCTCATCGTGGTC
CCTGAGATCTTCCTGCCCATCGAGGATGAGTGCGGCGATGTTCTGGTCATGAGGAAGAGT
GCCTCTCCCACGTCCATCACCACCTATGAGACCTGCCAGACCTACGAGAGGCCCATCGCC
TTCACCTCCCGCTCCCGCAAGCTCTGGATCCAGTTCAAATCCAATGAAGGCAACAGCGGC
AAAGGCTTCCAAGTGCCCTATGTCACCTACGATGAGGACTACCAGCAACTCATAGAGGAC
ATCGTGCGCGATGGGCGCCTGTACGCCTCGGAGAACCACCAGGAAATTTTGAAAGACAAG
AAGCTGATCAAGGCCCTCTTCGACGTGCTGGCGCATCCCCAGAACTACTTCAAGTACACA
GCCCAGGAATCCAAGGAGATGTTCCCACGGTCCTTCATCAAACTGCTGCGCTCCAAAGTG
TCTCGGTTCCTGCGGCCCTACAAA

Protein sequence predicted based on nucleotide sequence

MGAAAVRWHLCVLLALGTRGRLAGGSGLPGSVDVDECSEGTDDCHIDAICQNTPKSYKCL
CKPGYKGEGKQCEDIDECENDYYNGGCVHECINIPGNYRCTCFDGFMLAHDGHNCLDVDE
CQDNNGGCQQICVNAMGSYECQCHSGFFLSDNQHTCIHRSNEGMNCMNKDHGCAHICRET
PKGGVACDCRPGFDLAQNQKDCTLTCNYGNGGCQHSCEDTDTGPTCGCHQKYALHSDGRT
CIETCAVNNGGCDRTCKDTATGVRCSCPVGFTLQPDGKTCKDINECLVNNGGCDHFCRNT
VGSFECGCRKGYKLLTDERTCQDIDECSFERTCDHICINSPGSFQCLCHRGYILYGTTHC
GDVDECSMSNGSCDQGCVNTKGSYECVCPPGRRLHWNGKDCVETGKCLSRAKTSPRAQLS
CSKAGGVESCFLSCPAHTLFVPDSENSYVLSCGVPGPQGKALQKRNGTSSGLGPSCSDAP
TTPIKQKARFKIRDAKCHLRPHSQARAKETARQPLLDHCHVTFVTLKCDSSKKRRRGRKS
PSKEVSHITAEFEIETKMEEASDTCEADCLRKRAEQSLQAAIKTLRKSIGRQQFYVQVSG
TEYEVAQRPAKALEGQGACGAGQVLQDSKCVACGPGTHFGGELGQCVSCMPGTYQDMEGQ
LSCTPCPSSDGLGLPGARNVSECGGQCSPGFFSADGFKPCQACPVGTYQPEPGRTGCFPC
GGGLLTKHEGTTSFQDCEAKVHCSPGHHYNTTTHRCIRCPVGTYQPEFGQNHCITCPGNT
STDFDGSTNVTHCKNQHCGGELGDYTGYIESPNYPGDYPANAECVWHIAPPPKRRILIVV
PEIFLPIEDECGDVLVMRKSASPTSITTYETCQTYERPIAFTSRSRKLWIQFKSNEGNSG
KGFQVPYVTYDEDYQQLIEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYT
AQESKEMFPRSFIKLLRSKVSRFLRPYK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3055
Start on reference sequence 89
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable