NDST4 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NDST4
Gene Name:  N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4
Original Clone ID:
FLH269962.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297194
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2616nts
Open reading frame: 1 to 2616

ATGAATCTTATTGTGAAACTTCGGAGAAGTTTTCGAACATTGATTGTTCTCTTAGCTACC
TTTTGCTTGGTGAGCATTGTCATTTCTGCCTATTTTCTCTACTCTGGCTACAAACAGGAA
ATGACACTTATTGAAACCACTGCAGAAGCAGAATGCACTGACATCAAAATTCTACCATAT
AGGTCAATGGAGCTGAAAACAGTTAAACCTATTGACACATCCAAAACGGACCCTACTGTC
CTTCTCTTCGTGGAGAGCCAATACTCTCAACTCGGTCAAGATATCATAGCTATTTTGGAG
TCCAGCCGATTTCAGTACCACATGGTTATTGCCCCTGGAAAGGGAGATATACCTCCTCTT
ACAGATAATGGCAAAGGGAAATATACTTTAGTTATTTATGAAAATATTCTGAAGTATGTC
AGCATGGACTCATGGAATCGAGAGCTTTTAGAAAAATACTGTGTGGAATACAGTGTTAGT
ATAATCGGTTTTCATAAAGCCAATGAGAACAGCTTACCAAGTACACAATTAAAAGGCTTT
CCGTTAAACCTTTTCAATAATCTAGCTCTAAAGGACTGTTTTGTTAACCCTCAATCTCCT
TTGCTGCATATTACCAAAGCCCCCAAGGTTGAGAAAGGCCCTCTTCCTGGGGAAGACTGG
ACTATTTTCCAATATAATCATTCAACCTACCAGCCTGTACTTTTAACTGAGTTACAGACA
GAAAAATCCCTGTCATCCTTGTCTAGCAAAACACTCTTTGCAACGGTGATTCAGGATCTG
GGGCTTCATGATGGAATTCAGAGAGTACTTTTTGGCAACAACTTGAACTTTTGGCTGCAC
AAGCTCATCTTCATAGATGCCATCTCCTTCTTGTCAGGGAAGAGGCTGACATTGTCCTTG
GACAGGTACATCCTTGTGGACATTGATGATATATTTGTTGGGAAAGAGGGAACAAGGATG
AATGTCAAAGATGTGAAGGCATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCA
AATTTTACCTTCAACCTTGGATTTTCAGGGAAGTTTTACCACACAGGGACTGAAGAGGAA
GATGAAGGAGATGACCTTTTACTTCGGTCTGTGGATGAGTTCTGGTGGTTTCCTCACATG
TGGAGCCACATGCAGCCCCACCTCTTCCACAACGAGTCATCTTTAGTAGAGCAGATGATT
CTCAACAAGGAATTTGCACTGGAACATGGAATACCAATCAACATGGGCTATGCTGTGGCC
CCACATCACTCAGGGGTCTACCCGGTTCACATTCAGCTGTATGCAGCTTGGAAGAAGGTC
TGGGGTATTCAAGTCACCAGCACTGAAGAATATCCACATCTGAAACCTGCCCGGTACAGA
AAGGGCTTCATTCACAATAGCATCATGGTCCTCCCTCGACAGACTTGTGGGTTGTTCACT
CACACTATTTTCTACAAAGAATATCCAGGAGGACCCCAAGAACTGGATAAAAGTATCAGA
GGAGGTGAACTTTTTCTCACAATCCTTCTAAACCCAATCAGCATTTTCATGACCCATTTA
TCAAACTATGGAAATGACCGCCTAGGGTTATATACCTTTGTGAACTTGGTCAACTTTGTG
CAGAGCTGGACCAACCTGAAATTGCAAACTCTGCCTCCAGTGCAACTGGCCCACCAGTAT
TTTGAGCTCTTCCCTGAGCAGAAAGACCCTCTATGGCAGAATCCATGTGATGATAAACGA
CACAAAGACATCTGGTCCAGAGAGAAAACTTGTGACCACTTACCAAAATTTCTAGTAATT
GGTCCACAAAAAACAGGAACAACTGCACTTTATTTATTTCTTCTTATGCATCCTTCAATC
ATCAGCAATCTCCCTAGTCCAAAGACATTTGAGGAAGTTCAGTTCTTTAATGGCAACAAC
TATCACAAAGGAATAGACTGGTATATGGACTTTTTCCCTACACCATCCAATACTACAAGT
GACTTTCTGTTTGAAAAGAGTGCTAATTACTTCCATTCGGAAGAAGCTCCAAGACGAGCC
GCATCTCTTGTCCCCAAAGCCAAGATCATCACCATCCTCATTGACCCCTCAGACAGGGCA
TACTCTTGGTACCAGCACCAACGATCACATGAAGATCCAGCTGCTCTGAGGTTCAATTTC
TATGAAGTTATTTCAACAGGACATTGGGCTCCATCTGACTTAAAAACTTTGCAGAGAAGA
TGCCTAGTACCTGGATGGTATGCAGTCCACATAGAAAGATGGCTAACTTACTTTGCTACT
TCTCAGTTGCTAATTATTGATGGACAGCAGCTGAGATCTGACCCAGCTACTGTGATGGAT
GAAGTCCAGAAGTTTCTGGGAGTTACACCTCGTTATAATTACTCTGAAGCACTAACGTTT
GATCCCCAAAAGGGTTTTTGGTGTCAATTACTGGAAGGAGGAAAGACAAAATGCCTTGGA
AAAAGCAAAGGCCGAAAATATCCACCTATGGATCCAGAGTCTAGAACGTTCCTCTCTAAT
TACTACCGAGATCATAATGTGGAACTATCCAAACTGCTACACAGACTGGGACAGCCTCTG
CCATCGTGGCTGAGACAGGAACTGCAGAAAGTGAGA

Protein sequence predicted based on nucleotide sequence

MNLIVKLRRSFRTLIVLLATFCLVSIVISAYFLYSGYKQEMTLIETTAEAECTDIKILPY
RSMELKTVKPIDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYHMVIAPGKGDIPPL
TDNGKGKYTLVIYENILKYVSMDSWNRELLEKYCVEYSVSIIGFHKANENSLPSTQLKGF
PLNLFNNLALKDCFVNPQSPLLHITKAPKVEKGPLPGEDWTIFQYNHSTYQPVLLTELQT
EKSLSSLSSKTLFATVIQDLGLHDGIQRVLFGNNLNFWLHKLIFIDAISFLSGKRLTLSL
DRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSGKFYHTGTEEE
DEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINMGYAVA
PHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFT
HTIFYKEYPGGPQELDKSIRGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLVNFV
QSWTNLKLQTLPPVQLAHQYFELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVI
GPQKTGTTALYLFLLMHPSIISNLPSPKTFEEVQFFNGNNYHKGIDWYMDFFPTPSNTTS
DFLFEKSANYFHSEEAPRRAASLVPKAKIITILIDPSDRAYSWYQHQRSHEDPAALRFNF
YEVISTGHWAPSDLKTLQRRCLVPGWYAVHIERWLTYFATSQLLIIDGQQLRSDPATVMD
EVQKFLGVTPRYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDPESRTFLSN
YYRDHNVELSKLLHRLGQPLPSWLRQELQKVR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3298
Start on reference sequence 680
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable